OBJECTIVETo explore the relationship of TCM type with serum level of soluble CD44v6 (sCD44v6) and different histologic parameters in gastric carcinoma patients and to observe the influence of Weitai capsule (WTC) on the sCD44v6 expression.

METHODSTCM typing and sCD44v6 expression were determined in all the enrolled patients (30 in the control and 32 in the trial group) before operation, and 3-4 courses of chemotherapy was applied to them from 3-4 weeks after operation. To the patients of trial group, oral administration of WTC was given additionally with 4 capsules, 3 times a day for consecutive 3 months.

RESULTSsCD44v6 was significantly positive correlated with the degree of cancer cell differentiation, infiltration and lymph node metastasis; (2) Level of sCD44v6 was the highest in patients of blood stasis type, as compared with that in the patients of Pi-deficiency type or of damp-heat type, the difference was significant; (3) After ending treatment, level of sCD44v6 in the trial group was significantly lower than that in the control group.

CONCLUSION(1) Serum level of sCD44v6 could be taken as the criterion for evaluating the development and prognosis of gastric cancer, as well as the therapeutic target for anti-metastasis treatment; (2) Serum level of sCD44v6 is related to some extent with TCM type of blood stasis and Pi-deficiency; (3) WTC combined with chemotherapy could further inhibit the expression of serum sCD44v6 in gastric carcinoma patients.

Adenocarcinoma ; drug therapy ; immunology ; Adult ; Aged ; Antineoplastic Agents, Phytogenic ; therapeutic use ; Capsules ; Cell Adhesion ; Diagnosis, Differential ; Drugs, Chinese Herbal ; therapeutic use ; Glycoproteins ; blood ; Humans ; Hyaluronan Receptors ; blood ; Medicine, Chinese Traditional ; Middle Aged ; Phytotherapy ; Stomach Neoplasms ; drug therapy ; immunology

Objective To observe the signal transducers and activator of transcriptions (STATs) protein expression changes and investigate the functional role of STATs pathway in case of high glucoseinduced cardiac fibroblasts (CFs) proliferation and collagen deposition in vitro.Methods Rat cardiac fibroblasts were isolated from 1-to 3-day-old SD rats,cells from the second to fourth passages were used for the experiment.CFs were cultured in Dulbecco's modified Eagle's medium,supplemented with 5.5 mmol/L glucose(NG),5.5 mmol/L glucose plus 19.4 mmol/L mannose(OC) or 25 mmol/L glucose(HG) in the presence of absence of STAT1 inhibitor (fludarabine,FLU) and STAT3 inhibitor (S3I-201).After 24 h and 48 h culture in vitro,the proliferation of CFs was measured by 3-(4,5-dimethyl-2 thiazoyl)-2,5-diphenyl2H-tetrazolium bromide (MTT) assay.After 12 h and 24 h culture in vitro,the production of type Ⅰ and Ⅲ collagen was evaluated using real-time quantitative PCR and ELISA.After 0,30,60 and 120 min culture in vitro,the phosphorylated expression of STAT1 and STAT3 was analyzed by Western blot.Results CFs proliferation was significantly enhanced post 24 h and 48 h HG stimulation,and procollagen Ⅰ and Ⅲ mRNA expression was significantly upregulated post 12 h and 24 h HG stimulation.Deposition of collagen Ⅰ and Ⅲ was also significantly increased post 24 h and 72 h HG stimulation.STAT1 phosphorylation in CFs was increased after 120 min HG stimulation and STAT3 phosphorylation in CFs was increased post 60 min and 120 min HG stimulation.FLU and S3I-201 could inhibit HG-induced CFs proliferation and suppress of which was stimulated by FLU and S3I-201 could both suppress upregulated procollagen Ⅰ and Ⅲ mRNA expression and the deposition of collagen types Ⅰ and Ⅲ post HG stimulation.STAT1 phosphorylation inhibition resulted in less mRNA downregulation of procollagen type Ⅱ than that of procollagen type Ⅰ post 12 h HG stimulation.The STAT3 phosphorylation inhibition resulted in more significantly upregulated procollagen type Ⅲ mRNA expression than procollagen type Ⅰ mRNA expression at 12 h post HG stimulation.Conclusion HG could enhance the protein expression of phosphorylated STAT1 and STAT3 in CFs,which are responsible for HG-induced increased CFs proliferation and collagen deposition in vitro.

Objective To study influence of the mothers' accompany on pediatric anesthesia during venous revive recovery.Methods 58 cases with intravenous anesthesia pediatric surgery were enrolled and randomly divided into observation group and control group (29 cases in each group), the observation group was accompanied with mother in the anesthesia awaking room, and children in control group were not accompanied with their mothers.Children's restless, psychological status and revive period were observed in the two groups.Results Restless during revive period in observation group was significantly lower than control group (20.7% vs 69.0% ,x2 = 13.66,P ＜0.01) ;Fear,anxiety rate was lower than the control group(20.7% vs 89.7% ,x2 =27.88,P ＜0.01); BP、HR between two groups were significant difference (P ＜ 0.01).Conclusions Mother can significantly reduce anxiety, fear and restless incident rate of pediatric anesthesia.

Objective To discuss the effect of mind-body relaxing therapy on the eye pressure and physiological status of patients with glaucoma after surgery.Methods 86 patients with glaucoma were randomly divided into the research group and the control group, each with 43 cases. The control group received conventional medication treatment,while the research group received mind-body relaxing therapy in addition.SCL-90 was used to test two groups' physiological status,and their eye pressure was also measured and compared.Results The total score,average score,positive item quantity,body,press,depression,anxiety,opposition,horror,crankiness and lunacy score of SCL-90 of the research group were respectively (128.98 ±37.08),(1.59 ±0.41),(23.93 ±17.12),(1.35 ±0.81),(1.79 ±0.68),(2.25 ±0.91),(2.23 ±0.92),( 1.68 ± 0.82),(2.32 ± 0.92),( 1.88 ± 0.90),( 1.96 ± 0.92 ),all of which were lower than those of the control group [ ( 154.35 ± 37.82),( 1.88 ± 0.42 ),( 35.92 ± 17.56 ),( 1.90 ± 0.88 ),( 2.25 ± 0.71 ),( 2.88 ±0.95 ),(2.96 ± 0.96),( 2.26 ± 0.88 ),(2.98 ± 0.98 ),( 2.59 ± 0.93 ),(2.66 ± 0.95 ) ],and the differences were statistically significant ( u =3.14,3.24,3.21,3.01,3.07,3.14,3.60,3.16,3.22,3.60,3.47,respectively;P ＜0.01 ).The eye pressure of the research group was (18.10± 2.56)mm Hg,obviously lower than that of the control group which was ( 22.20 ± 2.68 ) mm Hg,and the difference was statistically significant ( u =7.25,P ＜ 0.01 ).Conclusions The mind-body relaxing therapy can evidently eliminate patients 'psychological barrier to recover their eye pressure and increase their confidence in the treatment,so as to promote their recovery and maintain their health to the maximum extend.

OBJECTIVETo study the expression and function of zinc ribbon gene ZNRD1 in drug-resistant cells of gastric cancer.

METHODSTwo tumor cell lines were used in this study: gastric cancer SGC7901 and its drug-resistant counterpart SGC7901/VCR stepwise-selected by vincristine. The expression of ZNRD1 in SGC7901 and SGC7901/VCR was detected by northern blot and semiquantitative RT-PCR. ZNRD1 antisense nucleic acid was transfected into SGC7901/VCR cells by lipofectamine. The expression of protein in SGC7901/VCR cells and the transfectants was detected by immunochemical method. Fluorescence activated cell scan (FACS) was applied to observe the cell cycle alteration. Growth curve and drug sensitization of cells for vincristine (VCR) and adriamycin (ADM) were analyzed by MTT assay.

RESULTSThe expression of ZNRD1 was higher in SGC7901/VCR than in SGC7901 cells. Immunochemical results showed that the expression level of ZNRD1 protein was lower in anti ZNRD1-SGC7901/VCR cells than in non-transfectants. The anti ZNRD1-SGC7901/VCR cells were gradually accumulated in G(1) phase, with a concomitant decrease of cell population in S phase. MTT assay showed that transfectant cell proliferation was lagged and more sensitive to VCR and ADM than non-transfectants.

CONCLUSIONZNRD1 gene displays high expression in VCR resistant gastric cancer cells. Expression of ZNRD1 protein is effectively blocked in anti ZNRD1-SGC7901/VCR cells by gene transfection. ZNRD1 antisense nucleic acid could reverse, to some degree, the MDR of human drug-resistant gastric cancer cell SGC7901/VCR.

Cell Cycle ; drug effects ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; DNA-Binding Proteins ; analysis ; genetics ; physiology ; Doxorubicin ; pharmacology ; Drug Resistance, Neoplasm ; Humans ; Stomach Neoplasms ; chemistry ; drug therapy ; pathology ; Vincristine ; pharmacology

OBJECTIVETo observe the effect of acupuncture on the expression of extracellular signal-regulated protein kinases 1/2 (ERK1/2) in the subcutaneous fascia of SD rats.

METHODSEighteen SD rats were randomly divided into 6 groups (n=3) including 5 acupuncture groups and a control group. The rats in the 5 acupuncture groups received electro-acupuncture therapy in the regions of the inguinal groove, and at 0, 1, 6, 12, and 36 h after the last therapy, the superfacial fascia surrounding the acupuncture point (about 1.5 cm in diameter) were collected. The fascia tissues at the corresponding sites and at the acupoint Zusanli (ST36) were obtained from the control rats. The expression of ERK1/2 and phosphorylated ERK1/2 (p-ERK1/2) in the tissues were detected by Western blotting.

RESULTSERK1/2 and p-ERK1/2 expressions were detected in the tissues harvested from both the acupoint and the non-acupoint in the control rats with similar expression intensities. In the rats of each acupuncture group, ERK1/2 expression was significantly increased on the acupuncture side in comparison with the control side.

CONCLUSIONThe normal loose connective tissue may participate in tissue proliferation and differentiation possibly via phosphorylation of ERK. Acupuncture can promote the signal transduction pathway of ERK, which can be a possible mechanism for the effect of acupuncture in modulating the physiopathological conditions.

Acupuncture Points ; Acupuncture Therapy ; Animals ; Blotting, Western ; Fascia ; enzymology ; Female ; Gene Expression Regulation, Enzymologic ; Mitogen-Activated Protein Kinase 1 ; metabolism ; Mitogen-Activated Protein Kinase 3 ; metabolism ; Rats ; Rats, Sprague-Dawley ; Skin ; Time Factors

Human infections with Lophomonas blattarum are rare. However, the majority of the infections occurred in China, 94.4% (136 cases) of all cases in the world. This infection is difficult to differentiate from other pulmonary infections with similar symptoms. Here we reported a case of L. blattarum infection confirmed by bronchoalveolar lavage fluid smear on the microscopic observations. The patient was a 21-year-old female college student. The previous case which occurred in Chongqing was 20 years ago. We briefly reviewed on this infection reported in the world during the recent 20 years. The epidemiological characteristics, possible diagnostic basis, and treatment of this disease is discussed in order to provide a better understanding of recognition, diagnosis, and treatment of L. blattarum infection.
Female ; Humans ; Lung Diseases, Parasitic/diagnosis/*parasitology ; Parabasalidea/*isolation & purification ; Protozoan Infections/*parasitology ; Young Adult

OBJECTIVETo investigate the relationship between 8 endocrine-disrupting chemicals in the serum and insulin resistance in patients with polycystic ovary syndrome (PCOS).

METHODSThis study was conducted among 60 patients with PCOS, including 23 with insulin resistance (PCOS-IR) and 37 without insulin resistance (PCOS-NIR), and 29 non-PCOS women seeking medical attention for infertility or menstrual disorder (control group). The serum levels of 6 phthalic acid esters (PEAs), bisphenol A (BPA) and octylphenol (OP) were measured in all the subjects.

RESULTSThe levels of PAEs, BPA and OP showed no significant differences between PCOS patients and the control group (P>0.05). The serum level of OP was significantly lower in patients PCOS-IR than in those with PCOS-NIR (47.89 ng/ml vs 60.24 ng/ml, P<0.05).

CONCLUSIONPEAs and BPA do not produce obvious effect on the pathogenesis of PCOS or contribute to insulin resistance, but OP may play a role in insulin resistance in PCOS patients.

Adult ; Benzhydryl Compounds ; adverse effects ; blood ; Case-Control Studies ; Endocrine Disruptors ; adverse effects ; blood ; Environmental Pollutants ; adverse effects ; blood ; Female ; Humans ; Insulin Resistance ; Phenols ; adverse effects ; blood ; Phthalic Acids ; adverse effects ; blood ; Polycystic Ovary Syndrome ; blood ; physiopathology ; Young Adult

OBJECTIVETo investigate the effect of gene Af116609 on gastric cancer multi-drug resistance (MDR) by introducing it into gastric cancer multi-drug resistant (MDR) cell line SGC7901/VCR.

METHODSGene Af116609 was cloned from SGC7901/VCR by RT-PCR and its differential expression between gastric cancer MDR cells and its parental cells was displayed by Northern blot. The gene was introduced to gastric cancer cells by transfection of recombinant eukaryotic expression vector by electroporation. MTT assay in vitro was applied to investigate its effect on multi-drug resistance phenotype of gastric cancer cells.

RESULTSThe full length CDS of gene Af116609, as long as 327 bp, was cloned from gastric cancer MDR cell line SGC7901/VCR and its sequence was coincident with the hypothetical gene Af116609 in GenBank. It was overexpressed in MDR cells than its parental cells at mRNA level. In the MTT assay in vitro, the drug sensitive cells transfected with sense eukaryotic expression vector showed upregulated targeted gene, with increased resistance to vincristine, 5-fliorouracil and arabinoside, and decreased resistance to adriamycin, but no influence on resistance to methotrexate. However, the drug resistant cells transfected with anti-sense eukaryotic expression vector, showed down regulated targeted gene, with less resistance to all the five anticancer drugs to different degrees.

CONCLUSIONGene Af116609 is related to MDR phenotype of gastric cancer cells and may become a candidate molecular target to reverse the MDR of gastric cancer.

Antineoplastic Agents, Phytogenic ; pharmacology ; Autoantigens ; genetics ; Cell Line, Tumor ; Drug Resistance, Multiple ; genetics ; Drug Resistance, Neoplasm ; genetics ; Humans ; RNA, Small Cytoplasmic ; genetics ; Ribonucleoproteins ; genetics ; Stomach Neoplasms ; genetics ; pathology ; Vascular Endothelial Growth Factor A ; biosynthesis ; Vincristine ; pharmacology

OBJECTIVETo study the epidemiological characteristics and molecular phenotypes of Salmonella by pulsed-field gel electrophoresis (PFGE) in Beijing from 2008 to 2009.

METHODSA total of one hundred thirty-seven isolates recovered from the WHO Global Salmonella Surveillance system and entero clinic surveillance system were identified by biochemical tests and serotyping. The related epidemiological informations were also analyzed. The isolates were further typed by PFGE.

RESULTSThe prevalence of Salmonella from 2008 to 2009 showed obvious seasonal character. High incidence occurred from June to September, and 64.1% (84/131) isolates were recovered in this period. Patients of 18 - 40 year-old were 46.1% (58/128) and 80 patients were male and 40 patients were female with the ratio of 1.57:1. These 137 Salmonella isolates belonged to 20 serotypes, including Enteritidis (46.7%, 64/137) and Typhimurium (17.5%, 24/137) as the dominant serotype. In total, 71 PFGE profiles were identified. Four PFGE patterns of S. Enteritidis isolates (JEGX01.CN0001, JEGX01.CN0003, JEGX01.CN0002, JEGX01.CN0019) and S. Typhimurium pattern of JPXX01.CN0001 were dominant patterns.

CONCLUSIONThe prevalence of Salmonella from 2008 to 2009 showed distribution characteristics of sex, age and seasons. The numerous PFGE patterns of Salmonella showed diversity of these isolates and different clones existed in Beijing.

Adolescent ; Adult ; Bacterial Typing Techniques ; China ; epidemiology ; DNA, Bacterial ; isolation & purification ; Electrophoresis, Gel, Pulsed-Field ; Female ; Food Microbiology ; Humans ; Male ; Molecular Typing ; Salmonella ; classification ; genetics ; isolation & purification ; Salmonella Infections ; epidemiology ; microbiology ; Serotyping ; Young Adult