Objective To explore the correlation between insulin resistance,inflammation factors and cognitive dysfunction in patients with metabolic syndrome (MS).Methods 150 cases of patients with MS were chosen,and they were divided into group A1 (n=68) and group A2 (n=82) according to whether they were with mild cognitive dysfunction (MCI).50 healthy cases chosen from physical examination were set as group B.The general data,laboratory examination results and inflammatory factor levels were compared between each group.MCI was set as dependent variable,insulin sensitivity index(ISI),high sensitive C-reactive protein(hs-CRP),and tumor necrosis factor-α(TNF-α) were set as independent variables.The relationship between inflammatory factor,insulin resistance and MCI was analyzed with multiple factors logistic regression analysis.Results The difference of body mass index(BMI),total cholesterol (TC),triglyceride (TG),low density lipoprotein (LDL),highdensity lipoprotein (HDL),fasting blood glucose(FBG),hemoglobin A1c (HbA1c),fasting insulin (FINS),and ISI between the three groups had statistical significance (P＜0.05).The levels of HbAlc in group A1 and group A2 were significantly higher than those in group B (P＜0.05).FBG and FINS showed a trend of reduce among the three groups,while ISI showed a trend of increase.The difference had statistical significance between each group (P＜0.05).The difference of hs-CRP,TNF-α between the three groups had statistical significance (P＜0.05).Levels of hs-CRP and TNF-α in group A1 were significantly higher than those in group A2 and group B,while the levels of hs-CRP and TNF-α in A2 group was significantly higher than those in group B (P＜0.05).Point biserial correlation analysis showed that the risk of MCI for MS patients had positive correlation with FBG,FINS,hs-CRP and TNF-α(r=0.219,0.231,0.309 and 0.287,P＜0.05),while it had negative correlation(r=-0.294,P＜0.05) with ISI.Multiariable logistic regression analysis showed that the more severe of insulin resistance (the lower ISI),the higher of inflammatory factor hs-CRP and TN-o,the greater the risk of MCI in patients with MS.Conclusion Insulin resistance and inflammation degree are more obvious in patients with MS combined with MCI,which can predict risk of cognitive dysfunction in patients with MS.

The human proinsulin gene was introduced a cleanage site for furin with two point mutation by polymerase chain reaction. The mutated gene was subcloned to the retroviral vector, and transferred into HepG2 hepatocarcinoma cells. Mature insulin can be expressed and secreted into supernatant by transferred HepG2 cells. The site mutation method is concise and with no misproduction.

Objectives To understand the current situation and evaluation about the employer's demand of knowledge level and comprehensive ability of professional undergraduate talents in the health management. Methods Using self-designed questionnaire, the investigation was carried out on the company executives and teachers from 12 health management-related enterprises where health management graduates of Hangzhou Normal University practice internships . A total of 31 valid questionnaires were recovered . SPSS 19.0 software was used to conduct descriptive analysis, hierarchical analysis and rank-sum test of one-way ordinal data. Results Employers believe that students need to strengthen the knowledge of health man-agement and medical knowledge, and the top three skills that students are most likely to improve are common disease management skills, health risk factors intervention skills and health monitoring skills. Amongst those areas of professional abilities that need improving, three of the most important skill sets include suffi-cient fundamental knowledge in medicine and pharmacology, analytical and problem-solving abilities and teamwork as well as communication skills. There is a significant difference among the level of students' pro-fessional knowledge, the level of their professional skills and the level of their comprehensive ability by the rank-sum test (P<0.05). Conclusion According to the results of the demand-side's survey, training of health management undergraduate talents should increase the proportion of teaching about medical theory knowledge, redesign practical teaching modules, carry out foreign language learning as well as promoting the training method of health talents by the means of information, in order to optimize the training of profes-sional health talents through multi-angle as well as multi-level.

Objective: To investigate the status of hair loss and analyze the influencing factors among university students in Hangzhou City, so as to provide insights into the management of hair loss among university students. Methods: University students were recruited using a convenient sampling method from 4 universities in Hangzhou City in June 2021. The basic characteristics and life styles were collected using online questionnaire surveys. Self-reported hair loss was evaluated using the grading scales for loss of hair (Hamilton-Norwood scale for males and modified Ludwig scale for females), and factors affecting self-reported hair loss were identified among university students using the multivariable logistic regression model. Results: A total of 1 060 questionnaires were allocated, and 1 038 valid questionnaires were recovered, with an effective recovery rate of 97.92%. The respondents included 391 males ( 37.67% ) and 647 females ( 62.33% ), and 463 respondents ( 44.61% ) reported hair loss, including 431 students with mild hair loss ( 93.09% ). Multivariable logistic regression analysis showed that university students in their fourth or fifth years ( OR=1.721, 95%CI: 1.126-2.630 ), art specialty ( OR=0.411, 95%CI: 0.207-0.816 ), overweight or obesity (OR=1.685, 95%CI: 1.050-2.704), diet taste ( sweet: OR=2.131, 95%CI: 1.370-3.316; spicy: OR=1.510, 95%CI: 1.028-2.218; greasy: OR=3.023, 95%CI: 2.015-4.537 ), feeling nervous/anxious (occasionally: OR=1.891, 95%CI: 1.087-3.289; frequently: OR=2.487, 95%CI: 1.337-4.626 ), smoking ( occasionally: OR=1.906, 95%CI: 1.067-3.405; frequently: OR=1.983, 95%CI: 1.050-3.746), family history of hair loss ( OR=1.506, 95%CI: 1.075-2.110 ), perming/dyeing hair ( occasionally: OR=1.795, 95%CI: 1.280-2.517; frequently: OR=3.282, 95%CI: 1.736-6.204), self-perceived oily hair/scalp in the past three months (slightly increased: OR=1.980, 95%CI: 1.477-2.653; significantly increased: OR=5.347, 95%CI: 2.956-9.670) were factors affecting self-reported hair loss among university students. Conclusion The proportion of self-reported hair loss was 44.61% among university students in Hangzhou City, and hair loss was predominantly mild. A family history of hair loss, nervousness/anxiety, diet habits, smoking and frequency of perm/dyeing hair may affect hair loss among university students.

Objective:To compare the differences of lung function parameters of the patients with congenital scoliosis (CS)or idiopathic scoliosis (IS),and to clarify their influence in respiratory function.Methods:Sixty-one patients of scoliosis undergoing operation were selected.According to the cause they were divided into 25 patients with CS and 36 patients with IS.The changes of lung function parameters and the differences in the patients with scoliosis were analyzed. Results:The ages,body weights, Cobb angles of the patients in two groups had no significant differences.There were 6 cases and 12 cases of ventilation disturbance in CS and IS groups,and 15 cases and 25 cases of ventilation dysfunction.The levels of residual volume (RV),ratio of residual volume to total lung capacity (RV/TLC),RV/TLC percent predicted (RV/TLC% pred )in two groups were higher than the normal. The percentage of forced vital capacity predicted (FVC%pred)and FEV1/FVC in CS group were both lower than those in IS group (P<0.05).Some pulmonary function parameters such as vital capacity (VC%pred),FVC%pred,forced expiratory volume in one second (FEV1%pred), maximal mid expiratory flow (MMEF%pred), maximal voluntary ventilation (MVV%pred),TLC%pred and diffusion capacity for carbon monoxide of the lung (DLCO%pred)were negtively correlated with Cobb angle (r=-0.54--0.35,P<0.05)in IS group and the TLC%pred was negtively correlated with Cobb angle (r=-0.047,P<0.05)in CS group.Conclusion:Different types of scoliosis have influence in the pulmonary function of the patients.The degree of pulmonary function injury of the patients with IS is worse than that of the CS patients.The IS patients need treatment in time.

Objective To detect the expression levels of adipose-specific phospholipase A2 (AdPLA) mRNA in orbital adipose tissue of thepatients with thyroid associated ophthalmopathy and the normals.Methods Sixteen patients with TAO of m level and stationary phase underwent orbital decompression,and 29 normals underwent ocular plastic surgery in Shenzhen Eye Hospital between August,2015 and October,2016.Orbital fat samples were collected from one eyes of these patients during surgery.The age,gender,height,weight,body mass index (BMI),exophthalmos degree,orbital fat of the patients with TAO and the normals were recorded and calculated.Using real time PCR,the AdPLA mRNA were detected from these orbital fat samples.Results There was no significant difference between the patients with TAO and the normals in age,gender,and BMI (all P ＞ 0.05).TAO group had more exophthalmos degree (20.406 ± 1.369)mm than the normals (14.207 ± 1.146) mm.TAO group had more orbital fat (32.162 ± 1.923) mL than the normals (24.279 ± 1.070) mL.The average expression level of AdPLA in patients with TAO was 0.039 42 ± 0.009 85,and 0.004 42 ± 0.001 36 in the normal.There was significant difference between two groups (P ＜ 0.05).Conclusion The patients with TAO of Ⅲ level and stationary phase have more exophthalmos degree and orbital fat than the normals.AdPLA mRNA is higher expressed in orbital adipose tissue of the patients with TAO of Ⅲ level and stationary phase than the normals.The high expression of AdPLA may reduce lipolysis in the orbital adipose tissue,lead to fat accumulation in orbits,and aggravate exophthalmos of patients with TAO.

We used four strains (Y1, Y2, M1 and M2) to screen out the high lipid production strains. We first adopted cell morphology and cytochemical methods (Sudan III dyeing technique) to observe intracellular characteristics. Observation results indicated that M2 strain had the potential lipid accumulation capacity. To prove this, lipid content of these strains was determined by soxhlet extraction. One strain (M2) was found to produce lipids up to 53.09%. In order to increase the production of oleaginous microorganism, the effects of hydrolysate concentration, nitrogen source, pH, fermentation temperature and time on cell growth and lipid accumulation were studied. The optimal fermentation conditions were obtained as follows: corn starch byproduct hydrolysate concentration at 10(o) Bx as carbon source; NaNO3 as nitrogen source at 0.2%; initial pH of 6.0; temperature at 28 degrees C, cultivated for 6 d. Under these conditions, M2 strain accumulated lipids up to 75.21% on a cellular biomass basis with biomass yield of 30.40 g/L, and the corresponding lipid production reached 22.86 g/L. GC analysis demonstrated that the fatty acid composition of the lipid was similar to that of vegetable oil, which mainly contained 16-and 18-carbon fatty acids. Thus, microbial lipid is a promising material for biodiesel production, and its unsaturated fatty acid content reached around 68%. These unsaturated fatty acids show great potential applications in food, medicine and cosmetics industries.
Biomass ; Culture Media ; Fatty Acids ; analysis ; biosynthesis ; Fermentation ; Hydrogen-Ion Concentration ; Industrial Microbiology ; Starch ; metabolism ; Temperature ; Yeasts ; growth & development ; metabolism

Objective: To analyze the awereness and beliefs on sedentary behavior among college students and associated factors, so as to provide a reference for improving sedentary behavior. Methods: A total of 1 261 students from six universities in Hangzhou City, were selected using a convenience sampling method. Sedentary behavior and its influencing factors were analyzed using the Chi square test and binary Logistic regression, respectively. Results: The pass rate of the knowledge and belief scores were 33.2% and 55.5%, respectively. The average duration of sedentary behavior on weekdays and weedends were (6.73±3.03) and (6.22±3.41)h/d, respectively. The results of the binary Logistic regression analysis showed that the influencing factors of health related sedentary behavior included gender, grade, subject major, sports performance and belief score ( P <0.01). Female students were 2.18 times more likely to exhibit unhealthy sedentary behaviors than male students (95% CI =1.58-3.01); senior college students were less likely to exhibit unhealthy static behaviors than freshmen ( OR =0.38,95% CI =0.23-0.61); and sports majors were less likely to exhibit unhealthy sedentary behaviors than medical majors ( OR =0.10, 95% CI =0.03-0.34). Students with high sports performance were less likely to exhibit unhealthy sedentary behavior ( OR =0.63, 95% CI =0.48-0.83). In terms of belief scores, students who obtained a pass rate were less likely to exhibit unhealthy static behavior ( OR =0.67, 95% CI =0.52-0.87). Conclusion Sedentary behavior is common among college students, and is influenced by several factors influencing factors. Colleges and universities should pay attention to improving the knowledge and belief level of students’ sedentary behavior, promote healthy exercise habits, reduce the duration of static behavior, and maintain the health level of college students.

OBJECTIVETo provide the basis for improving utilization of Artemisia annua germplasm resources and breeding variety, the interrelations between artemisinin content, artemisinin yield and agronomic traits of A. annua were studied.

METHODThe artemisinin content and each agronomic trait of 63 A. annua germplasm resources were measured by the visual observation and measurement methods. And the correlation analysis, regression analysis and path analysis were adopted.

RESULTThe result showed that there were significant differences in the artemisinin content and yield of 63 germplasm resources from the main production region of A. annua. Correlation analysis showed that there were significantly positive correlation between leaf weight and artemisinin yield with stem and branch characters, but there were negative correlation between artemisinin content with leaf characters of A. annua plant. The artemisinin content of A. annua increased with the increasing of primary branch number, bottom secondary branch number, and bottom stem diameter, etc. On the other hand, it decreased with the increasing of top secondary branch number, secondary leaf axis length, and bottom branch diameter, etc. The artemisinin yield of A. annua increased with the increasing of artemisinin content, leaf weight, and bottom secondary branch number, etc., and decreased with the increasing of bottom branch diameter, middle secondary branch number, and stem weight, etc. Path analysis showed that the primary branch number and bottom secondary branch number had a direct positive effect on the artemisinin content of A. annua. But the top secondary branch number had a direct negative effect on the artemisinin content of A. annua. The leaf weight and artemisinin content had a direct positive effect on the artemisinin yield and the ratio of leaf/stem, branch weight and stem weight had a direct negative effect.

CONCLUSIONOn the breeding A. annua variety, it can take into account both high leaf yield and high artemisinin content. And it was strongly recommend that the plant with moderate plant height and crown, shortness pinnae and secondary leaf axis, less middle and top secondary branch, strong stem, higher primary branch number and bottom secondary branch number, and higher ratio leaf/stem could be selected for breeding new varieties with high leaf yield and high artemisinin content.

Artemisia annua ; chemistry ; growth & development ; Artemisinins ; analysis ; Biomass ; Plant Extracts ; analysis ; Plant Leaves ; chemistry ; growth & development ; Plant Stems ; chemistry ; growth & development

Objective The effect and application value of the treatment of Graves eye disease were discussed.Methods 82 patients with Graves ophthalmopathy were divided into observation group and control group by random number method . The control group was given hormone therapy , the observation group was given methylprednisolone combined with methotrexate therapy .The clinical effect was compared between the two groups . Results The total effective rate of the observation group was 87.80％,which was significantly higher than 68.29％of the control group(χ2 =4.555,P<0.05).After treatment,the thyroid volume,eye level of the observation group were (24.68 ±1.25) g,(17.13 ±1.01) mm,respectively,which of the control group were (28.84 ±2.89) g, (18.79 ±2.12)mm,respectively,and the differences between the two groups were statistically significant (t=8.459, 4.526,all P<0.05).In the observation group,there were 2 cases of diabetes,1 case of hypertension,4 cases of insomnia,4 cases with digestive tract.In the control group,1 case of diabetes,2 cases of hypertension,5 cases of insomnia,3 cases of gastrointestinal tract ,and the differences between the two groups were not statistically significant (χ2 =0.346,0.346,0.124,0.156,all P>0.05).Conclusion Methylprednisolone combined with methotrexate in the treatment of Graves ophthalmopathy can improve the treatment effect , reduce the degree of exophthalmus and thyroid volume,and without increase in adverse reactions ,it is worthy of popularization and application in clinic .