Objective To explore the correlation between insulin resistance,inflammation factors and cognitive dysfunction in patients with metabolic syndrome (MS).Methods 150 cases of patients with MS were chosen,and they were divided into group A1 (n=68) and group A2 (n=82) according to whether they were with mild cognitive dysfunction (MCI).50 healthy cases chosen from physical examination were set as group B.The general data,laboratory examination results and inflammatory factor levels were compared between each group.MCI was set as dependent variable,insulin sensitivity index(ISI),high sensitive C-reactive protein(hs-CRP),and tumor necrosis factor-α(TNF-α) were set as independent variables.The relationship between inflammatory factor,insulin resistance and MCI was analyzed with multiple factors logistic regression analysis.Results The difference of body mass index(BMI),total cholesterol (TC),triglyceride (TG),low density lipoprotein (LDL),highdensity lipoprotein (HDL),fasting blood glucose(FBG),hemoglobin A1c (HbA1c),fasting insulin (FINS),and ISI between the three groups had statistical significance (P＜0.05).The levels of HbAlc in group A1 and group A2 were significantly higher than those in group B (P＜0.05).FBG and FINS showed a trend of reduce among the three groups,while ISI showed a trend of increase.The difference had statistical significance between each group (P＜0.05).The difference of hs-CRP,TNF-α between the three groups had statistical significance (P＜0.05).Levels of hs-CRP and TNF-α in group A1 were significantly higher than those in group A2 and group B,while the levels of hs-CRP and TNF-α in A2 group was significantly higher than those in group B (P＜0.05).Point biserial correlation analysis showed that the risk of MCI for MS patients had positive correlation with FBG,FINS,hs-CRP and TNF-α(r=0.219,0.231,0.309 and 0.287,P＜0.05),while it had negative correlation(r=-0.294,P＜0.05) with ISI.Multiariable logistic regression analysis showed that the more severe of insulin resistance (the lower ISI),the higher of inflammatory factor hs-CRP and TN-o,the greater the risk of MCI in patients with MS.Conclusion Insulin resistance and inflammation degree are more obvious in patients with MS combined with MCI,which can predict risk of cognitive dysfunction in patients with MS.

Clinical medical workers should understand the pathology,and the cultivation of the clinical pathological quality needs to start from the undergraduate course of medical students.In the past 6 years,based on the platform of undergraduate pathology science and technology innovation,and histology research platform of Department of Pathology,and through the creation of the clinical pathology elective courses and other forms,the researchers have given medical undergraduates the systematical and standardized training of the concept,knowledge,skills and application of pathology to develop their clinical and pathological quality during basic courses,professional courses and internship stage of a medical undergraduate.Practice shows that the relevant teaching measures enable students to fully understand the importance of clinical pathology,and improve their ability to apply clinical and pathological knowledge to solve clinical problems.

Objective To investigate the curative effect of arthroscopic treatment for lateral dis-cord meniscus tear in children. Methods The study involved 229 child patients with lateral discord meniscal tear treated arthroscopically from March 1996 to March 2008. There were 133 males and 96 fe-males, at age of (9±3.5) years (range 4-14 years). The treatment methods included meniscus-plasty in 219 patients (including two children with postoperative recurrence), meniscus-plasty-suture in eight and partial meniscectomy in two. The function of the knees was assessed pre-operationally and post-operation-ally by using Lysholm score. Results Of all, 221 patients were followed up for a period of (65±48.9) months (range 5-144 months). The pain and swelling were alleviated, with obvious improvement in range of motion and knee function in all patients six months after operation. There were no complica-tions including intra-articular infection, iatrogenic injury, fibroarthritis or loss of range of motion. Ly-sholm score was (61.85±7.65) points (range 45-73) before operation, which was improved to (95.30±3.50) points (range 87-100 points) six months after operation (t= 26.778, P < 0.01). Conclusion Treatment of lateral discord meniscus tear under arthroscopic guidance in children has the advantages of minor trauma, satisfactory results, can maintain good shape of the meniscus and good func-tion of knee and reduce incidence rate of osteoarthritis.

Objective To identify the expression of transcription factor Sp1 in NK/T-cell lymphoma (NK/TCL) cell lines and to investigate the role of Sp1 in regulation of cell invasion. Methods Real-time PCR, immunofluorescence and Western blot were performed to detect the expression of Sp1 in NK/TCL cell lines SNK-1 and SNK-6 and normal NK cells. Expression levels of IGF-1R and MMP-2 were measured by real-time PCR and Western blot, respectively. Transwell assay was applied to observe the effects of mythramycin A(MIT) on cell invasion. Results Sp1 expression in mRNA and protein were over-expression in NK/TCL cell lines SNK-1 and SNK-6 when compared with normal NK cells. Inhibition of Sp1 by MIT remarkably reduced expression of IGF-1R and MMP-2 in SNK-1, SNK-6 and as a result, or significantly suppressed cell invasion. Expression levels of Sp1 mRNA in SNK-1 and SNK-6 were (9.4±0.3) and (10.6±0.3) foldsincrease as compared with that of control group, respectively (P=0.005 2, P=0.003 7). Levels of Sp1 protein were (5.4±0.3) and (8.6±0.5) foldsincrease times than control groups, respectively (P=0.008 3, P=0.006 9). Inhibition of Sp1 by MIT (100 nmol/L) remarkably reduced expression levels of IGF-1R mRNA by (83.9±3.7) % and (65.8±4.2) % (P = 0.008 2, P = 0.009 7) as compared with controls. Meanwhile, levels of IGF-1R protein were reduced by (51.5±7.1) % and (49.6±9.1) % (P = 0.017 8, P = 0.015 5) as compared with control group. Inhibition of Sp1 by MIT (100 nmol/L) significantly reduced cell invasion and MMP-2 expression in the two cell lines,the cell invasion rates were reduced by (29.6±6.4) % and (37.2±7.6) % (P =0.041 8, P = 0.037 2) in SNK-1 and SNK-6 as compared with control group. The MMP-2 protein levels were found to be (52.7±4.7) % and (29.7±5.6) % (P = 0.028 6, P = 0.020 2) of control group. Conclusion Sp1 is over-expressed in NK/TCL cell lines, and it promotes NK/TCL cell invasion by up-regulating IGF-1R and further increasing MMP-2 expression.

It was discovered in this experiment that 3 days after inoculation of the ascitic fluid from P_(388) leukemic mice, the positive percentages of AcP and ?-Gase of the lymphocytes within the TNC of the recipients were increased significantly, while the positive percentage of ANAE was decreased. All the positive percentages of the three enzymes of the thymocytes within the thymus were increased significantly, but no abnormal structural changes were seen in the thymus. No histological and cytochemical changes were found in the lymph nodes and the spleen. Nine days after inoculation, the leukemia cell infiltration, hemorrhage and cell degeneration occurred in all the thymus, lymph nodes and spleen of the mouse. The normal structures of the organs were destroyed. The positive percentages of the cytochemical reactions of the three enzymes in the lymphocytes were all increased significantly. At this stage, TNC was very difficult to be isolated from the thymus. The results indicate that thymus and TNC may have a close relationship with the development of this type of leukemia.

Objective:To compare the differences of lung function parameters of the patients with congenital scoliosis (CS)or idiopathic scoliosis (IS),and to clarify their influence in respiratory function.Methods:Sixty-one patients of scoliosis undergoing operation were selected.According to the cause they were divided into 25 patients with CS and 36 patients with IS.The changes of lung function parameters and the differences in the patients with scoliosis were analyzed. Results:The ages,body weights, Cobb angles of the patients in two groups had no significant differences.There were 6 cases and 12 cases of ventilation disturbance in CS and IS groups,and 15 cases and 25 cases of ventilation dysfunction.The levels of residual volume (RV),ratio of residual volume to total lung capacity (RV/TLC),RV/TLC percent predicted (RV/TLC% pred )in two groups were higher than the normal. The percentage of forced vital capacity predicted (FVC%pred)and FEV1/FVC in CS group were both lower than those in IS group (P<0.05).Some pulmonary function parameters such as vital capacity (VC%pred),FVC%pred,forced expiratory volume in one second (FEV1%pred), maximal mid expiratory flow (MMEF%pred), maximal voluntary ventilation (MVV%pred),TLC%pred and diffusion capacity for carbon monoxide of the lung (DLCO%pred)were negtively correlated with Cobb angle (r=-0.54--0.35,P<0.05)in IS group and the TLC%pred was negtively correlated with Cobb angle (r=-0.047,P<0.05)in CS group.Conclusion:Different types of scoliosis have influence in the pulmonary function of the patients.The degree of pulmonary function injury of the patients with IS is worse than that of the CS patients.The IS patients need treatment in time.

Objective: To investigate the mode of cell death caused by NDV strain Changchun and NDV strain Siping. Methods: Plaque formation, cell suppression test, gel electrophoresis, and TUNEL assay were used after the cells were infected by NDV. Results: The apparently pathological changes were observed in chicken embryo fibroblasts, BHK, Hela, Hep 2, HCT and OS 732 tumor cells, but not in Wish cells. The higher suppressed effect on tumor cells was found in the NDV strain Changchun than that in the NDV strain Siping. There was no dose effect relationship between NDV and tumor cell suppression, only optimum dose NDV could cause maximal tumor cells inhibitory effect. Conclusion: The mode of cell death might be different after infection of NDV. The NDV strain Changchun killed tumor cells mainly through apoptosis, while the NDV strain Siping killed tumor cells mainly through necrosis. \[

Objective: To analyze the awereness and beliefs on sedentary behavior among college students and associated factors, so as to provide a reference for improving sedentary behavior. Methods: A total of 1 261 students from six universities in Hangzhou City, were selected using a convenience sampling method. Sedentary behavior and its influencing factors were analyzed using the Chi square test and binary Logistic regression, respectively. Results: The pass rate of the knowledge and belief scores were 33.2% and 55.5%, respectively. The average duration of sedentary behavior on weekdays and weedends were (6.73±3.03) and (6.22±3.41)h/d, respectively. The results of the binary Logistic regression analysis showed that the influencing factors of health related sedentary behavior included gender, grade, subject major, sports performance and belief score ( P <0.01). Female students were 2.18 times more likely to exhibit unhealthy sedentary behaviors than male students (95% CI =1.58-3.01); senior college students were less likely to exhibit unhealthy static behaviors than freshmen ( OR =0.38,95% CI =0.23-0.61); and sports majors were less likely to exhibit unhealthy sedentary behaviors than medical majors ( OR =0.10, 95% CI =0.03-0.34). Students with high sports performance were less likely to exhibit unhealthy sedentary behavior ( OR =0.63, 95% CI =0.48-0.83). In terms of belief scores, students who obtained a pass rate were less likely to exhibit unhealthy static behavior ( OR =0.67, 95% CI =0.52-0.87). Conclusion Sedentary behavior is common among college students, and is influenced by several factors influencing factors. Colleges and universities should pay attention to improving the knowledge and belief level of students’ sedentary behavior, promote healthy exercise habits, reduce the duration of static behavior, and maintain the health level of college students.

OBJECTIVEIn order to provide a scientific fertilizer application for the standardized cultivation, the effects of phosphate (P) fertilizer on the active ingredients and antioxidant activities of Chrysanthemum morifolium were studied.

METHODPot experiment was adopted to study the effects of P supply on the yield and the content of flavonoids, chlorogenic acid, soluble sugar, soluble amino acids and crude protein of C. morifolium flower. And effects of P supply on the hydroxyl radical scavenging activity, superoxide anion radical scavenging activity, and 2, 2-diphenyl-1-picrylhydrazyl hydrate (DPPH) free radical scavenging activity of flower were researched too.

RESULTThe yield of C. morifolium dry flower increased 129. 94% when P fertilizer was applied. Appropriate application of P fertilizer could also significantly improve the content and accumulation of total flavonoids, chlorogenic acid and soluble sugar in C. morifolium. Thus, the inhibition rates of hydroxyl radical, superoxide anion radical and DPPH free radical of C. morifolium was increased. When the level of P supply exceeded 0.20 g P2O5 per plant, P had also negative influence on the yield and the content of active ingredients and the scavenging activity of hydroxyl radical, superoxide anion radical and DPPH free radical of C. morifolium. Furthermore, there were significant positive correlations between the content of total flavonoids and chlorogenic acid and the inhibition rate of hydroxyl radical, superoxide anion radical and DPPH free radical, respectively.

CONCLUSIONAppropriate application of P fertilizer could be beneficial to the increase the active components and antioxidant activity of C. morifolium. And recommended level of P fertilizer is 0.26-0.28 g x kg(-1).

Antioxidants ; analysis ; metabolism ; Chrysanthemum ; chemistry ; metabolism ; Fertilizers ; analysis ; Phosphates ; metabolism ; Plant Extracts ; analysis ; metabolism

Objective:To observe the clinical effect of prolonged photodynamic therapy (PDT) irradiation time combined with intravitreal injection of ranibizumab in the treatment of circumscribed choroidal hemangioma (CCH).Methods:A retrospective clinical study. From March 2012 to March 2018, 51 eyes of 51 patients diagnosed in Shenzhen Eye Hospital were included in the study. Among the patients, the tumor of 36 eyes were located in macular area, of 15 eyes were located outside macular area (near center or around optic disc). All patients underwent BCVA, color fundus photography, FFA, ocular B-scan ultrasonography and OCT examinations. The BCVA examination was performed using the international standard visual acuity chart, which was converted into logMAR visual acuity. OCT showed 48 eyes with macular serous retinal detachment. of 36 eyes with tumor located in macular area, the logMAR BCVA was 0.05±0.05, the tumor thickness was 4.5± 2.2 mm, the diameter of tumor was 9.7±3.6 mm. Of 15 eyes with tumor located outside macular area, the logMAR BCVA was 0.32±0.15, the tumor thickness was 3.8±1.4 mm, the diameter of tumor was 7.7± 1.9 mm. PDT was performed for all eyes with the irradiation time of 123 s. After 48 h, all patients received intravitreal injections of 0.5 mg ranibizumab (0.05 ml). At 1, 3 and 6 months after treatment, the same equipment and methods before treatment were used for related examination. BCVA, subretinal effusion (SRF), tumor leakage and size changes were observed. BCVA, tumor thickness and diameter before and after treatment were compared by t test. Results:At 6 months after treatment, the tumor was becoming smaller without scar formation. FFA showed that the blood vessels in the tumor were sparse compared with those before treatment, and the fluorescence leakage domain was reduced. OCT showed 43 eyes of macular serous detachment were treated after the combined treatment. The logMAR BCVA were 0.16±0.15 and 0.55±0.21 of the eyes with tumor located in or outside macular area, respectively. The difference of logMAR BCVA between before and after treatment was significant ( t=-2.511, -2.676; P=0.036, 0.040). Both the tumor thickness ( t=3.416, 3.055; P=0.011, 0.028) and diameter ( t=4.385, 4.171; P=0.002, 0.009) of CCH patients were significantly reduced compared with that before treatment. Conclusion:The tumor of CCH can be reduced by prolonged PDT irradiation time combined with intravitreal injection of ranibizumab.