Objective To investigate and analyze the antimicrobial unreasonable use of antibacterial drugs in the com-munity center and to explore the corresponding countermeasures according to the reasons in order to accumulate experi-ence for promoting the rational use of drugs. Methods A total of 27 community health centers in the city were randomly selected as the research object and 50 antibiotic prescriptions, randomly selected among the antibiotic prescriptions is-sued in every community health center in the year 2012, were checked. The unreasonable use of antibacterial drug was classified, summarized and analyzed in the audit process. The corresponding countermeasures were explored according to the causes of the irrational use of antibiotics and changes of the irrational use of antibiotics were compared before and after the implementation of countermeasures. Results 293 copies of antibiotic prescriptions among the 1350 copies of antibiotic prescriptions randomly selected in 2012 were found unreasonable after the review, which accounted for the entire21.7% of the prescribed doses. Among the 293 copies of antibiotic prescriptions, the unreasonable choice of an-tibacterial drug accounted for 13%, the irrational drug use combined with other drugs accounted for 20.8% and drugs repeatedly used accounted for 9.2%, unreasonable solvents accounted for 18.8% and the usage and amount of drugs, which were unreasonable, accounted for 38.2%. After the implementation of standard medication strategy in 2013, the rate of irrational drug use was significantly lower than that in 2012 (9.3% vs 21.7%,χ2=78.9,P<0.05). Conclusion Through the analysis of the statistics of irrational drug use in the community health center, the appropriate regulatory measures have been planed, which has laid the foundation for the choice of suitable antibiotics and adopt suitable medication method and has guaranteed the rationality and the scientific nature of clinical use of drugs.