Objective To analyse the clinical characteristics of primary extra-nodal malignant lymphoma of the ear, nose and pharynx in order to improve the diagnosis of the disease. Methods Clinical characteristics of primary extra-nodal malignant lymphoma of the ear, nose and pharynx were analysed retrospectively in 16 patients. Especially that of the middle ear and paranasal sinuses, which were rare in occurrence. Results There was one patient with lymphoma of the middle ear, presenting otalgia, chronic secretory otitis media (conductive hearing loss) and facial nerve palsy as main symptoms. Seven patients of lymphoma of tonsils showed hypertrophy of the tonsil and foreign body sensation in pharynx as main symptomatology. Two patients of lymphoma in the nose and paranasal sinus complained of bloody rhinorrhea, odorous discharge, and inferior turbinate hypertrophy. The patients suffering from nasopharyx lymphoma always complained of headache and bloody rhinorrhea. Oropharyngeal lymphoma always presented symptoms of pharyngalgia, fever, soft palate ulcer, and ozostomia. Conclusion The keys to make a correct diagnosis of extra-nodal lymphoma of the ear, nose, or pharynx are to become familiar with their clinical characteristics and to perform a prompt biopsy.

OBJECTIVE Study the role of mycoplasma infection and expression of Ki67 protein in the pathogenesis, development and prognosis of laryngeal carcinoma. METHODS Immunohistochemistry method was used to study 145 specimens of laryngeal carcinoma tissues, 25 specimens of precarcinoma tissues, 31 specimens of vocal cord polyps and 15 specimens of normal tissues adjacent to laryngeal carcinoma. RESULTS ①The positive rates of PD4 and Ki67 were 45.52%(66/145) and 82.76 % (120/145) in laryngeal carcinoma tissue, 16.00 % (4/25) and 32.00 % (8/25) in precarcinoma tissue, 12.90 % (4/31) and 22.58 % (7/31) in vocal cords polyps, 6.67 % (1/15) and 0 (0/15) in normal tissues adjacent to laryngeal carcinoma. ②The positive rates of PD4 and Ki67 were higher in the advanced laryngeal carcinoma cases than that in the early laryngeal carcinoma cases. The positive rates of PD4 and Ki67 were higher in laryngeal carcinoma cases with cervical metastasis than that laryngeal carcinoma cases without cervical metastasis(P

OBJECTIVE: To investigate the expression of TGF-beta1 and HSP70 in human laryngeal squamous cell carcinoma. METHOD: The expression levels of TGF-beta1 and HSP70 in 53 specimens of human laryngeal squamous cell carcinoma and 48 specimens of para-carcinoma were detected by immuno- histochemistry and computer assisted image analysis. RESULT: The expression of TGF-beta1 was decreased in carcinoma tissues compared with para-carcinoma tissues ( P < 0.05), while the expression of HSP70 was increased ( P < 0.05). Both the expression of TGF-beta1 and HSP70 were significantly correlated with the differentiation of laryngeal squamous cell carcinoma (P < 0.01 or P < 0.05). However, there was a negative correlation between TGF-beta1 and HSP70 (r = -0.87, P < 0.01). CONCLUSION TGF-beta1 and HSP70 play an important role in malignant behaviors of human laryngeal carcinoma.
Adult ; Aged ; Carcinoma, Squamous Cell ; metabolism ; pathology ; Female ; HSP70 Heat-Shock Proteins ; metabolism ; Humans ; Immunohistochemistry ; Laryngeal Neoplasms ; metabolism ; pathology ; Male ; Middle Aged ; Neoplasm Staging ; Prognosis ; Transforming Growth Factor beta1 ; metabolism

OBJECTIVE: To explore the expression of mycoplasma monoclonal antibody PD4 in different laryngeal carcinoma tissues and their clinical significance. METHOD: Using immunohistochemistry methods to detect 256 cases of laryngeal tissues, including 105 cases of laryngeal carcinoma tissues, 31 cases of neck metastasis lymph node tissues, 18 cases of precarcinoma tissues, 53 cases of vocal cord polyps, 20 cases of normal tissues adjacent to laryngeal carcinoma and 29 cases of normal tissues around laryngeal carcinoma. RESULT: (1) The positive rate of PD4 was 44.76% in laryngeal carcinoma, which was much higher than that in other laryngeal tissues (P < 0.01 or P < 0.05). (2) The PD4 expression in laryngeal carcinoma was higher in the third and fourth stages than in the first and second stages, higher with cervical metastasis than those without cervical metastasis and higher in the third and second histological grade than in the first histological grade (P < 0.01 or P < 0.05). (3) To 3 and 5 years survival, PD4 positive cases had lower chance than PD4 negative cases (P < 0.01). CONCLUSION These results implicate that the occurrence, development and prognosis of laryngeal carcinoma may be closely related to mycoplasma infection.
Adult ; Aged ; Aged, 80 and over ; Antibodies, Monoclonal ; Apoptosis Regulatory Proteins ; metabolism ; Carcinoma, Squamous Cell ; mortality ; pathology ; Female ; Humans ; Laryngeal Neoplasms ; mortality ; pathology ; Lymphatic Metastasis ; Male ; Middle Aged ; Mycoplasma Infections ; Neoplasm Staging ; Prognosis ; RNA-Binding Proteins ; metabolism

OBJECTIVE: Study on the role of mycoplasma infection and expression of CD44v6, CD44v9 protein in the pathogenesis, development and prognosis of laryngeal carcinoma (LC). METHOD: Immunohistochemistry was used to study 137 cases of laryngeal carcinoma tissues, 26 cases of precarcinoma tissues, 34 cases of vocal cord polypus and 15 cases of normal tissues adjacent to laryngeal carcinoma. RESULT: The positive rate of PD4 and CD44v6, CD44v9 was 45.3% (62/137), 72.3% (99/137) and 56.2% (77/137) in laryngeal carcinoma, which are much higher than those in non-carcinoma tissues. (2) The positive rate of PD4 and CD44v6. CD44v9 in the third and fourth stages of LC were higher than those in the first and second stages of LC. Laryngeal carcinoma with cervical metastasis had higher expression than those without cervical metastasis (P < 0.01). To 3 and 5 years survival, PD4 and CD44v6, CD44v9 positive cases had lower chance than those negative cases (P < 0.01 [Chinese character: see text] 0.05). (3) The overall positive rate of PD4 and CD44v6, CD44v9 was 27.7% (38/137) in laryngeal carcinoma tissues and 5.3% (4/75) in non-carcinoma tissues. CONCLUSION These results implicates that the pathogenesis, development and prognosis of laryngeal carcinoma maybe closely related to the high expression of PD4 and CD44v6, CD44v9 protein.
Adult ; Aged ; Antibodies, Monoclonal ; biosynthesis ; Apoptosis Regulatory Proteins ; metabolism ; Carcinoma, Squamous Cell ; metabolism ; microbiology ; pathology ; Female ; Humans ; Hyaluronan Receptors ; metabolism ; Laryngeal Neoplasms ; metabolism ; microbiology ; pathology ; Male ; Middle Aged ; Mycoplasma ; immunology ; Neoplasm Staging ; Prognosis ; RNA-Binding Proteins ; metabolism