OBJECTIVETo investigate the cardiotonic effect of active compoud from raw and processing aconite roots used rats with acute heart failure (AHF) as the experimental animals.

METHODIsolated toad cordis was employed to observe the cardiotonic effect and the AHF models were reproduced in rats by pentobarbital sodium to observe the hemodynamics marker acute heart failure, and elucidate cardiotonic effect.

RESULTThe DDA(s) and MDA-P improved contractile force obviously on isolated toad cordis (P < 0.05). And the most amplification of contractile force was 91.107% +/- 87.663% (P < 0.01). But the effect on isolated heart rates was not significant. The change of LVSP, +/- dp/dt(max), LVEDP were significant (P < 0.01, P < 0.001), and the HR increased evidently by adminstrate processing aconite roots active compounds after the agent in 5 min.

CONCLUSIONThere are cardiotonic effect on isolated toad cordis in rats with active compounds from raw and processing aconite roots.

Aconitum ; chemistry ; Animals ; Anura ; Cardiotonic Agents ; pharmacology ; Disease Models, Animal ; Drugs, Chinese Herbal ; pharmacology ; Heart ; drug effects ; physiopathology ; Heart Failure ; drug therapy ; physiopathology ; Heart Rate ; drug effects ; Humans ; In Vitro Techniques ; Plant Roots ; chemistry ; Rats ; Rats, Sprague-Dawley