Cyclosporin A (CsA) does not only exert a toxic effect on kidney parenchymal cells, but also protects them against necrotic cell death by inhibiting opening of mitochondrial permeability transition pore. However, whether CsA plays a role in hydrogen peroxide-induced kidney proximal tubular cell death is currently unclear. In the present study, treatment with CsA further increased apoptosis and necrosis in HK-2 human kidney proximal tubule epithelial cells during exposure to hydrogen peroxide. In addition, hydrogen peroxide-induced p53 activation and BH3 interacting-domain death agonist (BID) expression were higher in CsA-treated cells than those in non-treated cells, whereas hydrogen peroxide-induced activation of mitogen-activated protein kinases including p38, c-Jun N-terminal kinase, and extracellular signal-regulated kinase and activation of protein kinase B were not significantly altered by treatment with CsA. In oxidant-antioxidant system, reactive oxygen species (ROS) production induced by hydrogen peroxide was further enhanced by treatment with CsA. However, expression levels of antioxidant enzymes including manganese superoxide dismutase, copper/zinc superoxide dismutase, and catalase were not altered by treatment with hydrogen peroxide or CsA. Treatment with CsA further enhanced mitochondrial membrane potential induced by exposure to hydrogen peroxide, although it did not alter endoplasmic reticulum stress based on expression of glucose-regulated protein 78 and 94. Taken together, these data suggest that CsA can aggravate hydrogen peroxide-induced cell death through p53 activation, BID expression, and ROS production.
Apoptosis ; Catalase ; Cell Death ; Cyclosporine ; Endoplasmic Reticulum Stress ; Epithelial Cells ; Humans ; Hydrogen Peroxide ; Hydrogen ; JNK Mitogen-Activated Protein Kinases ; Kidney ; Membrane Potential, Mitochondrial ; Mitogen-Activated Protein Kinases ; Necrosis ; Permeability ; Phosphotransferases ; Proto-Oncogene Proteins c-akt ; Reactive Oxygen Species ; Superoxide Dismutase

Purpose: The purpose of this study was to translate Duke University Religion Index (DUREL) and the Daily Spiritual Experience Scale (DSES) into Korean and test the validity and reliability of the instruments. Methods: Korean version of DUREL and DSES were translated and back-translated by 6 bilingual experts and group discussions. Data analyses were performed using SPSS 26.0 and AMOS 22.0. Both exploratory and confirmatory factor analyses were conducted with two data sets of secondary analysis study to examine the factor structure and construct validity. Correlations with other variables were used to test validity and reliability. Results: For K-DUREL, the final evaluation yielded two factors with 5 items: intrinsic religiosity and institutional religious activity. K-DSES had two factors with 16 items: transpersonal spiritual experience, and interpersonal & environmental spiritual experience. Cronbach’s ⍺ of K-DUREL and K-DSES were .67 and .96, respectively. Both instruments showed good reliability and validity for the translated Korean versions. Conclusion Applying K-DUREL and K-DSES in theory-based research may contribute to knowledge about the religious activity and spiritual experiences in the health and well-being of a Korean. Also, two instruments have the potential to measure changes in religion and spirituality after spiritual or holistic nursing interventions.

Purpose: The study aimed to translate the Couple Satisfaction Index (CSI 32) into Korean, to evaluate the reliability and validity of CSI 32 and short-form (CSI 16, 4) in the Korean context, and to determine a cut-off score for Korean couples. Methods: Korean Versions of the Couple Satisfaction Index (K-CSI) 32 was translated, back-translated, and reviewed by five bilingual experts. Confirmatory factor analysis (CFA) was conducted with data from a sample of 218 couples (N = 436) to test construct validity. Validity and reliability were evaluated. The receiver’s operating characteristics curve analysis was used to obtain the cut-off score. Results: The construct validities of K-CSI 32, 16, and 4 were verified using one-factor structures. The results of CFA showed a slightly better fit for K-CSI 16 and 4 than for K-CSI 32. Convergent validity was supported by significant positive correlations of K-CSI with Kansas Marital Satisfaction Scale, Dyadic Adjustment Scale, and Family Relationship Assessment Scale. Moreover, the significant differences in K-CSI between normal and depressive group demonstrated known-group validity. Cut-off scores of 105.5 on K-CSI 32, 50.25 on K-CSI 16, and 13.25 on K-CSI 4 were validated to identify distressed couple relationships. Conclusion For clinical practice, the reliable and valid K-CSI 32 has the potential to measure changes in couple satisfaction after couple therapy or interventions. Applying K-CSI 32 may facilitate research on couple and family relationships in nursing and contribute to the discussion on the role of couple satisfaction in mental health.

Purpose: The purpose of this study was to translate Duke University Religion Index (DUREL) and the Daily Spiritual Experience Scale (DSES) into Korean and test the validity and reliability of the instruments. Methods: Korean version of DUREL and DSES were translated and back-translated by 6 bilingual experts and group discussions. Data analyses were performed using SPSS 26.0 and AMOS 22.0. Both exploratory and confirmatory factor analyses were conducted with two data sets of secondary analysis study to examine the factor structure and construct validity. Correlations with other variables were used to test validity and reliability. Results: For K-DUREL, the final evaluation yielded two factors with 5 items: intrinsic religiosity and institutional religious activity. K-DSES had two factors with 16 items: transpersonal spiritual experience, and interpersonal & environmental spiritual experience. Cronbach’s ⍺ of K-DUREL and K-DSES were .67 and .96, respectively. Both instruments showed good reliability and validity for the translated Korean versions. Conclusion Applying K-DUREL and K-DSES in theory-based research may contribute to knowledge about the religious activity and spiritual experiences in the health and well-being of a Korean. Also, two instruments have the potential to measure changes in religion and spirituality after spiritual or holistic nursing interventions.

Purpose: This quasi-experimental single group study aimed to confirm the effects of discharge education using a systematic discharge education program on anxiety and parenting confidence in mothers of premature babies. Methods: This study conducted discharge education for 3 to 5 days prior to the discharge of 29 mothers of premature babies born in the neonatal intensive care unit. Data were collected between April 1, 2021, to June 30, 2021, and were examined. The hypotheses were analyzed using the Wilcoxon signed-rank test. Results: Discharge education using a systematic discharge education program was effective in increasing the parenting confidence of mothers with premature babies (z=-3.839, p<0.001). However, it was not effective in reducing anxiety (z=-1.712, p=0.087). Conclusion The effects of the systematic discharge education program development and discharge education systematized discharge nursing education, reduced mothers’ anxiety in raising premature babies at home after discharge, and contributed to improving parenting confidence.