1.Case of Intraneural Neurofibroma.
Byoung Dae KIM ; You Chan KIM ; Seung Hun LEE
Annals of Dermatology 2004;16(4):169-172
No abstract available.
Neurofibroma*
2.A Case of Microscopic Polyangiitis with Acute Myocardial Infarction.
Kyeong Han YOON ; Dae Hwan KIM ; Kyeong Han YOON ; Eun So LEE ; You Chan KIM
Annals of Dermatology 2002;14(3):181-185
Microscopic polyangiitis is a systemic small-vessel vasculitis, which may involve multiple organs, but cardiac involvement is relatively rare. We report a case of microscopic polyangiitis with multiple organ involvement, in which myocardial infarction was the early manifestation of the disease. A 53-year-old man presented with sudden papulovesicular eruptions and swellings on the face, posterior neck, dorsa of both hands and fingers, and with diffuse erythematous patches on the back. He had suffered from renal dysfunction, arthralgia, and hypertension for more than 8 years. He had been admitted to the department of cardiology for acute myocardial infarction and had suffered from recurrent gastrointestinal bleeding, renal failure, acute pancreatitis and sepsis during the admission. Histopathologically, small-vessel leukocytoclastic vasculitis with out granuloma was seen. Direct immunofluorescence showed no immune deposite. A high serum level of P-ANCA was detected by ELISA.
Acute Kidney Injury
;
Antibodies, Antineutrophil Cytoplasmic
;
Arthralgia
;
Cardiology
;
Enzyme-Linked Immunosorbent Assay
;
Fingers
;
Fluorescent Antibody Technique, Direct
;
Granuloma
;
Hand
;
Hemorrhage
;
Humans
;
Hypertension
;
Microscopic Polyangiitis*
;
Middle Aged
;
Myocardial Infarction*
;
Neck
;
Pancreatitis
;
Sepsis
;
Vasculitis
3.Effects of Extracts of Natural Products on Alkaline Phosphatase Activity of MC3T3-E1 Cells.
Sang Kee PARK ; Dae Kyum KIM ; Seung Han YOU ; Hyun A KIM ; Myoung Dong KIM ; Hyung Keun YOU ; Hyung Shik SHIN
The Journal of the Korean Academy of Periodontology 2001;31(1):123-134
Several growth factors and polypeptides were studied for the regeneration of periodontal supporting tissues which had been lost due to periodontal disease. But these are not commonly used for regenerators of bone tissue or alveolar bone, because of the insufficiency of studies on their side effects, genetic engineering for mass production and stability for clinical application. Recently, many natural products, which have advantage of less side effects and possibility of long-term use, have been studied for their capacity and effects of anti-bacterial, anti-inflammatory and regenerative potential of periodontal tissues. Cnidii Rhizoma, Rhinocerotis Cornu and Drynariae Rhizoma have been traditionally used as a drug for treatment of bone disease in oriental medicine. The purpose of this study was to examine the ability of alkaline phosphatase synthesis of MC3T3-E1 cells when above medicines were supplimented. MC3T3-E1 cells were cultured with alpha-MEM(negative control), dexamethasone(positive control), and each natural products for 3 and 5 days. And then ALP synthesis was measured by spectrophotometer for enzyme activity and by naphthol AS-BI staining for morphometry. Except Cnidii Rhizoma, all of the natural products of this study induced higher activity of ALP synthesis than controls. Among them Drynariae Rhizoma induced the highest activity. In the aspects of culturing time, all medicines did not showed the difference between 3 and 5 days, but 10-7g/ml group of Rhinocerotis Cornu showed significant increase at 3 days than at 5 days. These results indicate that several natural products have a inducing ability of ALP synthesis on osteoblasts.
Alkaline Phosphatase*
;
Biological Products*
;
Bone and Bones
;
Bone Diseases
;
Genetic Engineering
;
Intercellular Signaling Peptides and Proteins
;
Medicine, East Asian Traditional
;
Osteoblasts
;
Peptides
;
Periodontal Diseases
;
Polypodiaceae
;
Regeneration
4.A Case of Rothmund-Thomson Syndrome.
Byoung Dae KIM ; You Chan KIM ; Soo Chan KIM
Korean Journal of Dermatology 2003;41(9):1206-1209
Rothmund-Thomson syndrome is a rare autosomal recessive genodermatosis, which is characterized by poikiloderma and photosensitivity with variable features including alopecia, sparse hair, short stature, skeletal abnormalities, juvenile cataracts, and an increased risk of developing skin and bone malignancies. A 19-year-old man presented with poikilodermatous skin change on the whole body since three months old, and accompanied by photosensitivity, alopecia, and brachydactyly. As far as we know, this is the first case report of Rothmund-Thomson syndrome in Korea.
Alopecia
;
Brachydactyly
;
Cataract
;
Hair
;
Humans
;
Korea
;
Rothmund-Thomson Syndrome*
;
Skin
;
Young Adult
5.Effects of Cyclosporin A on the Cell Cycle Regulation of Human Gingival Fibroblasts.
Sung Hee PI ; Dae Kyum KIM ; Tak KIM ; Yong Ouk YOU ; Hyung Keun YOU ; Hyung Shik SHIN
The Journal of the Korean Academy of Periodontology 2001;31(3):611-623
Cyclosporin A is a cyclic polypeptide produced by the metabolism of fungi. It is widely used at present as immunosuppressive treatment following organ transplants. It is also used to deal with autoimmune diseases such as rheumatoid arthritis or type II diabetes. Gingival hyperplasia is one of the most frequent side-effects associated with the prescription of Cyclosporin A. The mechanisms involved in Cyclosporin A induced gingival hyperplasia are not yet clear. In vitro Cyclosporin A promotes proliferation of gingival fibroblasts, that Cyclosporin A act as a mitogen. Its action is based on mitosis of gingival fibroblasts regulated by cell cycle regulatory proteins. It was the purpose of the present study to examine the effects of Cyclosporin A on human gingival fibroblasts by means of biological and biochemical criteria. In this present study, we examined change of cell proliferation, cell activity, cell viability and cell cycle progression after application of Cyclosporin A. We also examined expression of cell cycle regulatory proteins by western blot analysis. Human gingival fibroblasts were cultured for 48 hours with application of Cyclosporin A at concentrations of 0.01, 0.1, 1, and 10 ng/ml. Cyclosporin A(1 ng/ml) significantly increased the cell activity of gingival fibroblast. Proliferation and viability of gingival fibroblasts were also increased in group treated with 1 ng/ml of Cyclosporin A compared to control group. In the cell cycle analysis, S phase was increased and G1 phase was decreased in the group treated with 1 ng/ml of Cyclosporin A. Cyclosporin A increased the expression of cdk4 and inhibited the expression of pRB and p21. These results suggest that 1 ng/ml of Cyclosporin A may increase the cell cycle progression of human gingival fibroblasts, and its mechanisms may increase the expression of cdk4 and decrease the expression of pRB and p21.
Arthritis, Rheumatoid
;
Autoimmune Diseases
;
Blotting, Western
;
Cell Cycle Proteins
;
Cell Cycle*
;
Cell Proliferation
;
Cell Survival
;
Cyclosporine*
;
Fibroblasts*
;
Fungi
;
G1 Phase
;
Gingival Hyperplasia
;
Humans*
;
Metabolism
;
Mitosis
;
Prescriptions
;
S Phase
;
Transplants
6.The Relationship between ST-T Electrical Alternans on EKG and Ventricular Arrhythmia.
You Hong KIM ; Seong Yun O ; Dal Soo LIM ; Dae Seong HYUN ; Sung Gug CHANG ; Young Hoon YOU ; Young Jo KIM ; Bong Sup SHIM ; Hyun Woo LEE
Korean Circulation Journal 1990;20(3):305-314
In order to observe the development of ventricular arrhythmia during regional myocardial ischemia and reperfusion, especially under the presence or absence of ST-T electrical alternans on epicardial EKG. The proximal left descending coronary artery(LAD) was ligated for 20 minutes and then reperfused suddenly in twenty-three cats. Standard lead EKG(Lead??, chest lead EKG and epicardial lead EKG were recorded simultaneously during the occlusion and reperfusion respectively. During the ligation of LAD, STEA was observed in thirteen cats(56.5%). In occlusion period, the incidence of ventricular tachycardia in STEA positive group was significantly higher than in the negative group(p<0.01) and arrhythmic score was significantly higher(p<0.005) also In the reperfusion period the incidence of vefntricular fibrillation in STEA positive group was significantly higher than in the negative group(p<0.025). But there was the tendency to be higher in arrhythmic score of STEA positive group. There were no differences in heart rate, systolic left ventricular pressure, ST elevation and ST width in both groups. Most forms of ST-T of sinus rhythm before and after development of ventricular premature beat was low form(L). It was concluded that at the presence of STEA on EKG, the incidence of ventricular arrhythmia was more prevalent. So, STEA can be available as a marker of ventricular arrhythmia and prognostic factor.
Animals
;
Arrhythmias, Cardiac*
;
Cardiac Complexes, Premature
;
Cats
;
Electrocardiography*
;
Heart Rate
;
Incidence
;
Ligation
;
Myocardial Ischemia
;
Reperfusion
;
Tachycardia, Ventricular
;
Thorax
;
Ventricular Pressure
7.Effects of Replicative Senescence on the Cell Cycle Regulation in Human Gingival Fibroblasts.
Young Chae PARK ; Dae Seung YANG ; Jae Ho KIM ; Hyun A KIM ; Yong Ouk YOU ; Hyung Keun YOU ; Hyung Shik SHIN
The Journal of the Korean Academy of Periodontology 2001;31(1):135-147
Gingival fibroblasts are major cellular component of gingiva. However, the molecular mechanisms of senescence of human gingival fibroblasts are unknown. Human fibroblasts undergo replicative senescence in vitro after a limited number of population doublings. A reduced rate of proliferation is a prominent phenomenon observed in senescent fibroblasts. This phenomenon is happened with cell cycle arrest that was controled by cell cycle regulatory proteins. The purpose of present study was to investigate the effect of replicative senescence on cell cycle progression and to find out its molecular mechanisms in human gingival fibroblasts. Replicative senescence of gingival fibroblasts were induced by subsequent cultures that were repeated up to 18 passage. In the present study, I examined change of cell proliferation, cell activity, cell viability and cell cycle progression during the replicative process. Also, I examined expression of cell cycle regulatory proteins which was estimated by western blot analysis. Cell proliferation, cell activity and cell viability of gingival fibroblasts were notably decreased with increase of population doubling level(PDL). S phase was decreased and G1 phase was increased with increase of PDL. Western blot analysis showed that levels of p16, p21 and p53 of senescent gingival fibroblasts(PDL41, PDL58) were higher than young fibroblasts(PDL27) and cdk4 were lower than young fibroblasts(PDL27). In conclusion, these results suggest that proliferative function of human gingival fibroblasts may be decreased by replicative senescence and its molecular mechanisms may be activatied with p16, p21, p53 and pRB, and repressed wtih cdk4.
Aging
;
Blotting, Western
;
Cell Aging*
;
Cell Cycle Checkpoints
;
Cell Cycle Proteins
;
Cell Cycle*
;
Cell Proliferation
;
Cell Survival
;
Fibroblasts*
;
G1 Phase
;
Gingiva
;
Humans*
;
S Phase
8.Effects of Ginseng Radix on the Cell Cycle Regulation in Human Fetal Osteoblast.
Dae Gyeom KIM ; Yong Bae LEE ; Sang Kee PARK ; Hyung Keun YOU ; Kyung Tae YOU ; Yun Chul KIM ; Hyung Shik SHIN
The Journal of the Korean Academy of Periodontology 2003;33(3):415-437
Ginseng Radix(GR) had been used widely from oriental medicine and the effects of it have been investigated by many researchers. The purpose of present study was to investigate the effects of GR on the cell cycle progression and its molecular mechanism in human fetal osteoblast. The results were as follows. Increased cell proliferation was observed in cells exposed to 100 ng/ml, 10 ng/ml of GR-1 at 12 hours and 24 hours, 1 microgram/ml of GR-1 at 48 hours, and 100 microgram/ml, 10 microgram/ml of GR-2 at 12 hours, all treatment groups of GR-2 at 24 hours(p<0.05). S phase and G1 phase was increased in the group of treated with 100 ng/ml of GR-1, with 10 microgram/ml and 1 microgram/ml of GR-2, with 100 microgram/ml and 10 microgram/ml of GR-3 in the cell cycle analysis. The cell cycle regulation protein levels of Cyclin D1, Cyclin E, CDK 2, CDK 4 and CDK 6 were increased in the group of treated with 1 microgram/ml and 100 ng/ml of GR-1, with 10 microgram/ml and 1 microgram/ml of GR-2, with 100 microgram/ml and 10 microgram/ml of GR-3. On the other hand, p21 was decreased in the treatment group with 1 microgram/ml and 100 ng/ml of GR-1, with 10 microgram/ml and 1 microgram/ml of GR-2, 10 microgram/ml of GR-3, and p53 and p16 was decreased in the treatment group with 100 ng/ml of GR-1, 100 microgram/ml GR-3 and pRb was decreased in the all treatment groups except 1 microgram/ml of GR-1. These results suggested that GR increases the cell proliferation and the cell cycle progression in human fetal osteoblast, which is linked to increased cell cycle regulation protein levels of Cyclin D1, Cyclin E, CDK 2, CDK 4, CDK 6 and decreased cell cycle regulation protein levels of p21, pRb.
Humans
9.Surgical Treatment of Intracranial Arachnoid Cyst by Excision.
Hwa Seung PARK ; Dong Youl RHEE ; Sang Yong KIM ; Sang Nam PARK ; Dae Noung KIM ; Man Su YOU
Journal of Korean Neurosurgical Society 1991;20(10-11):847-853
Arachnoid cysts are benign developmental cysts that occur between aachnoid layers, which represent approximately 1 percent of all intracranial space-occupying lesions, their most common location being the middle cranial fossa. Current surgical treatment options for intracranial arachnoid cyst are either craniotomy and excision of cyst or shunting of cyst contents extracranially. We treated 10 cases of intracranial arachnoid cyst by excision during last two years. For the best surgical curability, we have, not only removed the cyst wall and adhesive gliotic tissue as possible, but also made the opening to basal cistern for communication. During average 8.4 months follow up, we observed, not specific morbidity and mortality, but good clinical improvement and brain expansion. As above result, if haven't specific problem, the direct surgical removal may be the primary choice for intracranial arachnoid cyst, especially in children with noncommunicating type in sylvian.
Adhesives
;
Arachnoid Cysts*
;
Brain
;
Child
;
Cranial Fossa, Middle
;
Craniotomy
;
Follow-Up Studies
;
Humans
;
Mortality
10.The detection of anti-ENA antibodies in systemic rheumatic diseases.
Sang Cheol BAE ; Gwan Gyu SONG ; In Hong LEE ; Dae Hyun YOO ; Seong Yoon KIM ; Think You KIM
Korean Journal of Medicine 1993;45(4):422-436
No abstract available.
Antibodies*
;
Rheumatic Diseases*