No abstract available.

The antigenic domain of the major surface protein (Nc-p43) of Neospora caninum was examined by polymerase chain reaction of its gene fragments and recombinant expression as GST fusion proteins. The fragments of Nc-p43 were as follow: a total open reading frame (OFR), T; OFR without signal sequence and C-terminal hydrophobic sequence, S; N-terminal 2/3 parts of S, A; C-terminal 2/3 parts, P; N-terminal 1/3 part, X; middle 1/3 part, Y; and C-terminal 1/3 part, Z, respectively. The DNA fragments were cloned into pGEX-4T vector. Recombinant plasmids transformed into Escherichia coli of BL21 pLysS (DE3) strain were induced to express GST or GST fused fragments of Nc-p43 such as 69 kDa protein for T, 66 kDa for S, 52 kDa for A, 53 kDa for P, and 40 kDa proteins for X, Y, and Z, respectively in SDS-PAGE. The Nc-p43 fragments of T, S, and P reacted with a bovine serum of neosporosis while those of A, X, Y, and Z together with GST did not in the western blot. These findings suggest that the antigenic domain of Nc-p43 of N. caninum may be localized in the C-terminal 2/3 parts. Together with A19 clone in SAG1 of Toxoplasma gondii (Nam et al., 1996), the P fragment of Nc-p43 could be used as efficient antigens to diagnose and differentiate those infections with both species.
Amino Acid Sequence ; Animals ; Base Sequence ; Biological Markers/analysis ; Blotting, Western ; Cells, Cultured ; Cercopithecus aethiops ; Coccidiosis/diagnosis ; Neospora/*immunology ; Protozoan Proteins/*analysis/genetics/immunology ; Reverse Transcriptase Polymerase Chain Reaction ; Support, Non-U.S. Gov't ; Vero Cells/parasitology

No abstract available.
Arthroplasty* ; Knee*

No abstract available.
Osteoporosis* ; Radionuclide Imaging*

OBJECTIVE: The cyclin-dependent kinase inhibitor p27kip1 protein is a negative regulator of the cell cycle, and its degradation is required for entry into the S phase. Loss of p27kip1 expression has been reported to be associated with aggressive behavior in a variety of tumors of epithelial and lymphoid origin. However, its association with various astrocytic tumors has not been clearly demonstrated. We studied to investigate the relationship of p27kip1 expression with the biological behavior of astrocytic tumors in addition to study on the role of p27kip1 in the tumorigenesis of these tumors. PATIENTS AND METHODS:From 1990 to 1998, a total of 29 astrocytic tumor of all grades obtained by operative resection were included for evaluation. We studied the expression of p27kip1 protein immunohistochemical assay in astrocytic tumors and compared the findings with the clinicopathologic parameters. Immunohistochemical staining was performed on formalin-fixed paraffin-embedded sections by the avidin-biotin-peroxidase complex method. According to WHO classification, all cases were divided into astrocytomas(4 cases), anaplastic astrocytomas(9 cases), and glioblastomas(16 cases) by 3 pathologists. Clinical information was obtained from medical records, and others such as location and size of tumors from imaging studies. RESULTS: Mean p27kip1 protein labeling indexes(LI, mean+/-standard deviation) of astrocytomas, anaplastic astrocytomas, and glioblastomas were 80.6+/-9.1, 63.6+/-21.0, and 28.9+/-18.7, respectively, and were inversely correlated with grade of glial tumors(p<0.0001). Mean p27kip1 protein LI in the recurrent group was lower than that in the non-recurrent group, but there was no significant difference statistically(p=0.464). Additionally, p27kip1 protein expression did not show any significant relationship to other prognostic factors such as age(p=0.1643), tumor size(p=0.8), or location(p=0.8). CONCLUSION: These results suggested that reduced expression of p27kip1 protein may play a important role in the malignant transformation process of astrocytic tumor cells.
Astrocytoma ; Carcinogenesis ; Cell Cycle ; Classification ; Cyclin-Dependent Kinase Inhibitor p27* ; Glioblastoma ; Humans ; Medical Records ; Phosphotransferases ; S Phase

PURPOSE: We report the first case in Korea of rapid bone formation on a subperiosteal orbital hematoma after trauma. CASE SUMMARY: A 10-year-old boy who was in the intensive care unit after trauma showed proptosis and ocular movement limitation of the right eye associated with subperiosteal hematoma. On ocular examination, 3 mm of proptosis and limitation of right eye movement were observed; however, visual acuity was not decreased. At 1 month after the trauma, orbital computed tomography (CT) showed new bone formation at the margin of the hematoma border although the size of the hematoma decreased. The patient underwent hematoma and bony tissue removal using anterior orbitotomy approach. A new bone was formed between the orbital border and hematoma from the anterior orbital margin to the orbital apex. During pathological examination, woven bone tissue with fibrotic tissue was observed in the hematoma wall. One year after surgery, the patient's proptosis and limitation of ocular movement disappeared without any evidence of new bone formation. CONCLUSIONS: Waiting for spontaneous absorption of orbital subperiosteal hematoma is usually recommended unless there is significant functional impairment. However, as in our case, new bone formation could occur during a short period of less than 1 month; imaging follow-up is necessary in patients having intensive care or showing delayed absorption of a hematoma.
Absorption ; Bone and Bones ; Child* ; Exophthalmos ; Eye Movements ; Follow-Up Studies ; Hematoma* ; Humans ; Critical Care ; Intensive Care Units ; Korea ; Male* ; Orbit* ; Osteogenesis ; Visual Acuity

We report a patient with idiopathic hypertrophic cranial pachymeningitis (IHCP) who presented with headache, tinnitus, hearing loss, dizziness and tongue atrophy. She initially was misdiagnosed as having an en plaque meningioma involving the posterior fossa and foramen magnum on the basis of brain MRI findings. A meningeal biopsy excluded meningioma and a diagnosis of IHCP was made. After immune modulating treatment, the patient's symptoms gradually improved. Our case shows IHCP should be included in the differential diagnosis of meningeal mass.
Atrophy ; Biopsy ; Brain ; Cranial Nerve Diseases* ; Cranial Nerves* ; Diagnosis ; Diagnosis, Differential ; Dizziness ; Foramen Magnum ; Headache ; Hearing Loss ; Humans ; Magnetic Resonance Imaging ; Meningioma* ; Meningitis* ; Tinnitus ; Tongue

No abstract available.
Stents* ; Ureter*

A 47-year-old man presented with recurrent transient right hemiparesthesia and right homonymous hemianopsia. The transient ischemic attacks (TIAs) may be related to hemodynamic disturbance caused by acute occlusion of the internal carotid artery with severe stenosis of the ipsilateral posterior cerebral artery. After urgent endovascular revascularization of the internal carotid artery, there were no further attacks. Our case suggests that repetitive sensory TIAs can be induced by a hemodynamic disturbance caused by a large artery stenosis, which can be prevented with urgent endovascular treatment.
Arteries ; Carotid Artery, Internal ; Cerebral Revascularization ; Constriction, Pathologic ; Hemianopsia ; Hemodynamics ; Humans ; Ischemic Attack, Transient* ; Middle Aged ; Posterior Cerebral Artery ; Stents

A lymphatic cyst of the stomach is rare benign tumor which is composed of thin-walled lymphatic space lined by benign-appearing endothelial cells. It rarely manifests clinical symptoms, so it is found incidentally in most cases. Recently, a case of submucosal lymphatic cyst associated with early gastric cancer was experienced. An endoscopy revealed a slightly elevated, edematous lesion adjacent to the malignant ulcer. This case is herein reported with a brief review of related literature.
Endoscopy ; Endothelial Cells ; Lymphocele* ; Stomach ; Stomach Neoplasms* ; Ulcer