Background: The insulin-like growth factors, IGF-I and
Animals ; Calcimycin ; Calcium ; Egtazic Acid ; Extracellular Fluid ; Glucose ; Hepatocytes ; Insulin ; Insulin-Like Growth Factor Binding Protein 3 ; Insulin-Like Growth Factor Binding Proteins ; Insulin-Like Growth Factor I ; Liver ; Metabolism ; Parathyroid Hormone ; Perfusion ; Rats ; Somatomedins

Bone grafts are used in the repair of segmental bone loss caused by severe trauma, bone tumors and infection, and to enhance bone healing in ununited fractures. Autograft is the most frequently used and the most effective method, but because of inadquate supply and additional operative morbidity, allograft or heterograft could be used. Heterograft has been shown to be poorly tolerated by the host and ineffective in providing an osteogenic system. The objective of this study is to observe healing process of a segmental defect of long bone following to heterograft, and to compare the difference of histologic process between autograft and hetero graft. Twenty-four white rabbits weighing 1,000 to 2,000 grams were used for the experiment. A segment measuring three times the diameter of the ulna shaft (1.5 to 2cm) of rabbit was resected with its periosteum. Twenty-four white rabbit were divided into three groups (control, autograft, heterograft group) according to graft methods, each group comprising of eight rabbits. The animals were sacrificed at 2, 4, 8 and 12 weeks after the experimental procedures and were periodically evaluated by radiographs and histology. The obtained results were as follows: 1. The results of the radiological evaluation showed that no ungrafted ulnar defects (control group) healed. In the heterograft group, partial union was observed from 8 weeks and complete union was obtained on 12 weeks. In the autograft group, partial union was observed on 2 weeks and complete union was obtained on 4 weeks. 2. The results from histological examination showed that the ungrafted ulnae did not heal across the defect but some immature trabeculae were founded on the edges of the defect. In the heterograft group, immature trabeculae were appeared from 4 weeks and defects were substituted with mature trabeculae on 12 weeks. In autograft group, defects were substituted with immature trabeculae on 2 weeks and with mature trabeculae on 4 weeks. As seen in the results of the experiment, union could be obtained with the heterograft, but needed more long time than autograft. Though the autograft is the most effective graft method in bone defect or non-union, above results suggest the heterograft as the alternative method in the treatment of large bone defect, in a multioperated patient, or in the children or the elderly, combined with autograft or alone.
Aged ; Allografts ; Animals ; Autografts ; Child ; Fractures, Ununited ; Heterografts* ; Humans ; Periosteum ; Rabbits* ; Transplants ; Ulna

Pretibial myxedema is a condition in which there is loeal thickening of the skin by a mucin-like deposit; it is nearly always asosciated with ophthalmopathy and thyrotoxicosis, not infrequently becomes more pronounced after treatrnent of thyrotoxicosis. The precise cause of pretibial myxedema is not known, but it appears that IgG LATS represents an autoantibody against a thyroid antigen, retroorbital tiesue and tbe skin, so, pretibial myxedema is presumed to be the result of a local antigen-antibody tissue reaction. A 57-year-old man had the history of diabetes since 1964 and Graves disease since May 1980, he was treated with metimazole for 1 month, with improving thyrotoxicosis but developed the pretibial myxedema. The histologic findings showed considerable amount of mucin, especially hyaluronic acid with toluidin blue stain at PH 3.0. The lesions were improved by local application of 0.01 x fluocinolone acetonide ointment with occlusive dressing technique.
Fluocinolone Acetonide ; Graves Disease ; Humans ; Hyaluronic Acid ; Hydrogen-Ion Concentration ; Immunoglobulin G ; Long-Acting Thyroid Stimulator ; Middle Aged ; Mucins ; Myxedema* ; Occlusive Dressings ; Skin ; Thyroid Gland ; Thyrotoxicosis

Lipomas are common soft tissue that are usually located in the subcutaneous tissue. And intramuscular lipomas commonly arise in the upper and lower extremities, where they usually involve the large muscles. Intramuscular lipoma, also referred to as an infiltrating lipoma, is an unusual benign slow growing tumor composed of mature fat cell interdigitating with skeletal muscle. However, intramuscular lipomas are exceedingly rare in the face. We have been experienced a case intramuscular lipoma that located in the malar area. Because of the rarity of these tumors and their propensity to recur without adequate surgery, the case report is presented here. Achievement of surgical margin is essential as the recurrent rate may be as high as 15% to 62.5% without complete excision.
Adipocytes ; Lipoma* ; Lower Extremity ; Muscle, Skeletal ; Muscles ; Subcutaneous Tissue

PURPOSE: Nocturnal Enuresis, an involuntary discharge of urine after bladder control, is frequent in children. It brings patients and family many psychogenic problems. So we investigated its clinical characteristics and drug effects. METHODS: The study subjects enrolled were 36 children with nocturnal enuresis. We evaluated family history, accompanying urinary symptoms and clinical characteristics through questionnaries. We evaluated the effect of imipramine and desmopressin in 26 children among the 36 cases. RESULTS: The ratio of boys to girls was 1.6 to 1. The age range in 15 cases(41.7%) was 4-5 years, in 14 cases(38.9%) 6-7 years, in 4 cases(11.1%) 8-9 years, and in 3 cases(9.3%) it was above 10 years. Twenty four(67%) were primary enuresis and 12(33%) were secondary enuresis. In eighteen cases(50%), these was a family history of enuresis. Seven of these cases had a parental enuresis history. The accompanying urinary symptoms were urgency(47.2%), frequency (38.9%), and dysuria(13.9%). The responses to imipramine were as follows : an excellent response in 2 cases(20%), a good response in 4(40%), a transient response in 2(20%), and no response in 2(20%). The responses to desmopressin were as follows : an excellent response in 7(43.8%), a good response in 4(25%), a transient response in 3(18.7%), and no response in 2(12.5%). CONCLUSION: Enuresis is more frequent in boys and primary type. Half of 36 enuresis children had a family history of enuresis. We recommend continuous drug medication to control nocturnal enuresis along with encouragement.
Child* ; Deamino Arginine Vasopressin ; Enuresis ; Female ; Humans ; Imipramine ; Nocturnal Enuresis ; Parents ; Urinary Bladder

PURPOSE: Nocturnal Enuresis, an involuntary discharge of urine after bladder control, is frequent in children. It brings patients and family many psychogenic problems. So we investigated its clinical characteristics and drug effects. METHODS: The study subjects enrolled were 36 children with nocturnal enuresis. We evaluated family history, accompanying urinary symptoms and clinical characteristics through questionnaries. We evaluated the effect of imipramine and desmopressin in 26 children among the 36 cases. RESULTS: The ratio of boys to girls was 1.6 to 1. The age range in 15 cases(41.7%) was 4-5 years, in 14 cases(38.9%) 6-7 years, in 4 cases(11.1%) 8-9 years, and in 3 cases(9.3%) it was above 10 years. Twenty four(67%) were primary enuresis and 12(33%) were secondary enuresis. In eighteen cases(50%), these was a family history of enuresis. Seven of these cases had a parental enuresis history. The accompanying urinary symptoms were urgency(47.2%), frequency (38.9%), and dysuria(13.9%). The responses to imipramine were as follows : an excellent response in 2 cases(20%), a good response in 4(40%), a transient response in 2(20%), and no response in 2(20%). The responses to desmopressin were as follows : an excellent response in 7(43.8%), a good response in 4(25%), a transient response in 3(18.7%), and no response in 2(12.5%). CONCLUSION: Enuresis is more frequent in boys and primary type. Half of 36 enuresis children had a family history of enuresis. We recommend continuous drug medication to control nocturnal enuresis along with encouragement.
Child* ; Deamino Arginine Vasopressin ; Enuresis ; Female ; Humans ; Imipramine ; Nocturnal Enuresis ; Parents ; Urinary Bladder

PURPOSE: We investigated the effectiveness and safety of DA-3030 for prophylatic use in patients receiving chemotherapy for malignant disease. MATERIALS AND METHODS: Seventy cancer patients were randomized to receive chemotherapy alone (36 patients) or with DA-3030 administered (34 patients) after stratified block randomization according to chemotherapeutic regimen. DA-3030 was subcutaneously administered at the dose of 100 pg/m/day for 10 days from 24 hours after the completion of chemotherapy. RESULTS: Of the 70 enrolled patients, 62 patients were evaluable. The neutropenia (absolute neutrophil count [ANC] <1,000/mm) occurred in 9 of 32 (28.1%) of the DA-3030 group and 21 of 30 (90.0%) of the control group, giving relative risk for control group of 0.154 (95% confidence interval [CI], 0.05 to 0.45; p-0.0001). Severe neutropenia (ANC 500/mm') occurred in 4 of 32 (12.5%) of the DA-3030 group and in 20 of 30 (66.7%) of the control group (relative risk for control group of 0.316 [95% CI, 0,18 to 0.55]; p=0.0001). The mean duration of neutropenic period (+/-standard error) was 1.13+/-0.34 days in the DA-3030 group and 6.73+/-0.69 days in the control group respectively, and was significantly shorter in the DA-3030 group (p<0.0001). And, there was higher nadir ANC in the OA-3030 group than that in the control group (p=0.0001); the mean nadir ANC was 2,547+/- 343/mm and 442+/-120/mm, respectively. The DA-3030 group had significantly higher incidence of myalgia in comparison to the control group (43.8% compared with 3.3%; p=0.001). However, it was tolerable and was easily managed by conservative therapy CONCLUSION: The use of DA-3030 was effective in preventing chemotherapy-induced neutropenia.
Drug Therapy* ; Humans ; Incidence ; Myalgia ; Neutropenia* ; Neutrophils ; Random Allocation

No abstract available.
Bites, Human* ; Herpes Simplex* ; Humans ; Simplexvirus

Extraskeletal chondromas are relatively uncommon benign cartilaginous tumors of the soft tissue and well known to pose a considerable diagnostic problem because of histological variations including the immature appearance of their tumor cells. Recently, we have experienced a case of extraskeletal chondroma mimicking benign chondroblastoma. The patient was a 47-year-old woman who complained of a painful subcutaneous swelling on the radial aspect of 4th proximal interphalangeal (PIP) joint in the left hand for 6 months. Radiologic examination of the 4th finger revealed a 1cm-sized soft tissue mass. Histologically, the tumor was characterized by a lobulated mass which was composed of dense proliferation of chondroblast-like cells admixed with a few multinucleated giant cells of osteoclastic type. However, there were focal areas of typical chondroma which showed lace-like intense calcification around the differentiated chondrocytes.
Chondroblastoma ; Chondrocytes ; Chondroma* ; Female ; Fingers ; Giant Cells ; Hand ; Humans ; Joints ; Middle Aged ; Osteoclasts

OBJECTIVES: The myelodysplastic syndromes (MDS) are a group of acquired clonal hematopoietic disorders characterized by peripheral cytopenias and a hypercellular or normocellular dysplastic bone marrow. The mechanisms responsible for development of MDS are not known. We performed this study to evaluate the hematopoietic abnormalities and apoptosis in MDS. METHODS: Long-term bone marrow culture (LTBMC) was performed for colony assays, cobblestone area assay, stromal morphologic changes from 7 patients with MDS and 7 normal controls. In situ nick end labeling (ISNEL) method was performed for detection of apoptosis from LTBMC in 7 patients with MDS and 7 normal controls. ISNEL method also performed in bone marrow cell bloc samples in 36 patients with MI3S. RESULTS: Viability of nonadherent cells from LTBMC of patients with MDS was not decreased compared with normal controls at 1 week, but significantly decreased at 2 and 3 weeks compared with normal controls (P<0.0001). Formation of the cobblestone areas from patients with MDS was slightly decreased compared with normal controls at 1st week, but significantly decreased at 2nd and 3rd weeks compared with normal controls (P<0.0001). Slightly decreased compared with normal controls at 1 week, but significantly decreased at 2 and 3 weeks compared with normal controls (P<0.0001). Stromal layers produced in LTBMC of normal controls and 1 patient with MDS were detected at 1 week and were formed confluent stroma from 3 weeks, but another patients with MDS who did not form a confluent stroma. Patients with MDS had significantly lower colony forming unit granulocyte-macrophage (CFU-GM) compared with normal controls at 1 (P<0.01) and 2 weeks (P<0.001) of LTBMC. Two weeks of LTBMC resulted more profound inhibition of CFU-GM formation than 1 week (P<0.0001). Apoptotic cell death was absent in adherent and non adherent cells from normal controls at 1 and 2 weeks, but massive apoptotic cell death was found in adherent and non adherent cells from patients with MDS at 1 and 2 weeks and the degree of apoptosis was profound at 2 weeks compared with 1 week. Among the 36 patients, fifteen patients demonstrated varying degrees of apoptosis positive cells, 4 having low, 8 intermediates, and 3 high scores. Remaining 21 patients showed absent apoptosis or only occasional positive cells. CONCLUSION: Hematopoietic abnormalities such as a failure of differentiation are caused by the stromal defects and the biologic basis of the apparent paradox of peripheral cytopenias in the face of hypercellular (or normocellular) marrow is related by intramedullary apoptotic cell death of the stromal and hematopoietic cells.
Apoptosis* ; Bone Marrow Cells ; Bone Marrow* ; Cell Death ; Granulocyte-Macrophage Progenitor Cells ; Humans ; In Situ Nick-End Labeling* ; Myelodysplastic Syndromes* ; Stem Cells