Although there are many reports on the splenic (systemic) T cell response after Toxoplasma gondii infection, little information is available regarding the local T cell responses of peritoneal exudate cells (PEC) and gut intraepithelial lymphocytes (IEL) following peroral infection with bradyzoites. Mice were infected with 40 cysts of the 76K strain of T. gondii, and then sacrificed at days 0, 1, 4, 7 and 10 postinfection (PI). The cellular composition and T cell responses of PEC and IEL were analyzed. The total number of PEC and IEL per mouse increased after infection, but the ratio of increase was higher in IEL. Lymphocytes were the major component of both PEC and IEL. The relative percentages of PEC macrophages and neutrophils/eosinophils increased significantly at day 1 and 4 PI, whereas those of IEL did not change significantly. The percentage of PEC NK1.1 and gamma delta T cells peaked at day 4 PI (p < 0.0001), and CD4 and CD8 alpha T cells increased continuously after infection. The percentages of IEL CD8 alpha and gamma delta T cells decreased slightly at first, and then increased. CD4 and NK1.1 T cells of IEL did not change significantly after infection. IFN-gamma-producing PEC NK1.1 T cells increased significantly from day 1 PI, but the other T cell subsets produced IFN-gamma abundantly thereafter. The proportion of IEL IFN-gamma-producing CD8 alpha and gamma delta T cells increased significantly after infection, while IEL NK1.1 T cells had similar IFN-gamma production patterns. Taken together, CD4 T cells were the major phenotype and the important IFN-gamma-producing T cell subsets in PEC after oral infection with T. gondii, whereas CD8 alpha T cells had these roles in IEL. These results suggest that PEC and IEL comprise different cell differentials and T cell responses, and according to infection route these factors may contribute to the different cellular immune responses.
Acute Disease ; Animals ; Ascitic Fluid/cytology/*metabolism ; Female ; Interferon Type II/*biosynthesis ; Intestinal Mucosa/cytology ; Lymphocytes/*metabolism ; Mice ; Mice, Inbred C57BL ; Support, Non-U.S. Gov't ; T-Lymphocyte Subsets/*immunology ; Toxoplasmosis/*immunology

Prevalence of sparganum in the frog (Rana nigromaculata) was surveyed in Dae-jeon city and in suburbs of the city, Chungnam Do. 39 (3.9 per cent) out of 1,011 frogs were infected with the worm, and the highest prevalence rate of sparganum was found the frogs from Shintanjin area(11.3 per cent). Infection rates of sparganum were observed due to the body weight and sex of frogs. The highest infection rate of frogs, the body weight of 50 gms or more, showed 30.3 per cent(female: 41.7 per cent, male: none), on the other hand, no worm was found in young frogs(less than 10 gms). Distribution of sparganum in the frogs was recorded and 82.6 per cent of worms were distributed in the femoral intermuscular connective tissues of the hind legs. Among those infected frogs, number of worm burden was one to five worms, and 53.3 per cent of frogs were infected by only one worm.
parasitology-helminth-cestoda ; Spirometra sp. ; sparganum ; sparganosis ; epidemiology ; frog ; Rana nigromaculata

This study was performed to observe the therapeutic effects of interferon-gamma(IFN-gamma) and gamma-globulin(gamma-globulin) in experimental Pneumocystis carinii pneumonia of immune suppressed mice. After 9 weeks, trimethoprim-sulfamethoxazole(TMP-SMZ; 10-50 mg/mouse/day), mouse IFN-gamma(5 x 10(4) units/mouse/day) and mouse gamma-globulin(20 mg/mouse/day) were administered to the mice for 3 weeks by the experimental group. The therapeutic efficacy was evaluated by body weights, histopathologic and electron microscopic findings of the lungs, and number of P. carinii cysts by Gomori's methenamine silver stain. Body weights of the mice were significantly increased in the group of combination therapy of TMP-SMZ with IFN-gamma or gamma-globulin, and in the group of TMP-SMZ treatment (p < 0.05), however, little effect was found in the group of gamma-globulin alone. Histopathologic findings of P. carinii pneumonia were much improved in the group of combination therapy of TMP-SMZ with IFN-gamma. Treatment with either TMP-SMZ or IFN-gamma significantly reduced the number of cysts in the P. carinii pneumonia, but gamma-globulin alone was ineffective. In electron microscopic findings of P. carinii pneumonia, the number of trophozoites and cysts were reduced by treatment with either TMP-SMZ or IFN-gamma, and most of the cysts were empty or containing one or two intracystic bodies. The present results suggested, that combination therapy of TMP-SMZ with IFN-gamma had synergistic effects in treatment of P. carinii pneumonia in experimental mice.
Drug-Synergism ; Drug-Therapy,-Combination ; English-Abstract ; Gamma-Globulins-administration-and-dosage ; Interferon-Type-II-administration-and-dosage ; Mice- ; Trimethoprim-Sulfamethoxazole-Combination-administration-and-dosage ; *Gamma-Globulins-therapeutic-use ; *Interferon-Type-II-therapeutic-use ; *Pneumonia,-Pneumocystis-carinii-therapy ; Gamma-Globulins ; Trimethoprim-Sulfamethoxazole-Combination ; Interferon-Type-II

Primary hemangiopericytoma of chest wall is very rate and only a few cases have ever been reported. The tumor aries from the capillary pericyte of Zimmerman. It is a highly vascular slow growing tumor which can be calssified as both malignant and benign varieties. We report a case of a 66 year-old man in whom recurrent hemangiopericytoma was treated by complete surgical excision. In October 1993 he had received surgical excision of hemangiopericytoma on posterior chest wall. For more than 6 years after the operation he was in good condition until a recurrent mass was found on the chest X-ray. The patient was discharged 9 days after the operation and is receiving radiotherapy.
Aged ; Capillaries ; Hemangiopericytoma* ; Humans ; Pericytes ; Radiotherapy ; Thoracic Wall* ; Thorax*

It is the purpose of this study to determine the benificial effect of treatment with methylprednisolone on the enhancement of Na+, K+-ATPase activity and the increased ATP level during the 4 hours after a 400gm-cm injury to the spinal cord of cat. These results demonstrate that high dose (30mg/kg) of methylprednisolone can benificially enhance the activity of neuronal Na+, K+-ATPase during the first 4 hour after spinal cord injury. But the enhancement of this enzyme activity is not significantly increased with mega dose (60mg/kg) of methylprednisolone. Tissue level of ATP in the high dose-treated cat at 1 hour after trauma was significantly elevated, but those in high dose-treated cat at 4 hour and the 1 and 4 hours with mega dose-treated groups were not significantly increased. The protective effects of methylprednisolone in experimental spinal cord trauma are the enhancement of Na+, K+-ATPase, inhibition of the free-radical reaction and the lipid peroxidation, the increment of neuronal activity and ATP utilization. High dose(30mg/kg) of methylprednisolone is required in order to produce the acute effect in the early phase experimental spinal cord injury.
Adenosine Triphosphate ; Animals ; Cats ; Lipid Peroxidation ; Methylprednisolone* ; Neurons ; Spinal Cord Injuries* ; Spinal Cord*

No abstract available.

No abstract available.
Constriction, Pathologic* ; Neurologic Manifestations* ; Spinal Canal*

No abstract available.
Head* ; Magnetic Resonance Imaging* ; Necrosis*

To evaluate the role of nitric oxide (NO) in IFN-gamma production and apoptosis of splenocytes in genetically different strains of mice with toxoplasmosis, BALB/c (a toxoplasmosis resistant strain) and C57BL/6 (a toxoplasmosis susceptible strain) mice were infected with Toxoplasma gondii cysts orally and subsequently injected intraperitoneally with aminoguanidine, an iNOS inhibitor (AG; 35 mg/kg per mouse daily for 14 days). When BALB/c or C57BL/6 mice were infected with T. gondii without AG treatment, number of brain cysts, NO and IFN-gamma production by splenocytes, and percentages of apoptotic splenocytes were increased compared to uninfected control mice without AG treatment. AG treatment increased the number of brain cysts, and reduced NO and IFN-gamma production in T. gondii-infected C57BL/6 mice. In contrast, in T. gondii-infected BABL/c mice, the number of brain cysts, and NO and IFN-gamma production of splenocytes was not altered by treatment with AG. However, the percentages of apoptotic splenocytes in T. gondii-infected BALB/c or C57BL/6 mice were not affected by AG treatment. These results suggest that NO modulates IFN-gamma production in T. gondii-infected C57BL/6 mice, and that NO is involved in mediating a protective response in toxoplasmosis susceptible, but not resistant, mice strain during acute infection.
Animals ; Apoptosis/drug effects/*immunology ; Comparative Study ; Female ; Guanidines/*pharmacology ; Interferon Type II/*biosynthesis ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Nitric Oxide/*biosynthesis ; Nitric-Oxide Synthase/*antagonists & inhibitors ; Research Support, Non-U.S. Gov't ; Species Specificity ; Spleen/immunology ; Toxoplasmosis, Animal/*immunology

BACKGROUND: Congenital cystic diseases of the lung are uncommon but they share similar embryogenic and clinical characteristics. MATERIAL AND METHOD: From 1972 to 1999, 46 patients were operated on in the Dept. of Thoracic Surgery at the YUMC. And we analysed the patients' charts for clinical presentations, managements and pathologic findings with chart of patients. RESULT: There were 17 males and 29 females, and mean age was 16.2 years. The main symptoms were 11 fevers due to recurrent infection, 10 dyspneas, and chest pain and sputum production etc. Thirteen patients were asymptomatic. There were 13 pulmonary sequestrations(PS), 12 congenital cystic adenomatoid malformations(CCAM), 12 bronchogenic cysts(BC), 5 cystic bronchiectasis(CB) and 4 congenital lobar emphysemas(CLE). Simple excision(resection) was performed in 8 patients, segmentectomy in 6 patients, lobectomy (with 1 operative mortality) in 31 patients, and pneumonectomy in one patient. The compli-cations were 4 pneumonias, one persistent air leakage and one empyema. CONCLUSION: We experienced surgical treatment of congenital cystic lung disease and reported them with literature review.
Chest Pain ; Dyspnea ; Empyema ; Female ; Fever ; Humans ; Lung Diseases* ; Lung* ; Male ; Mastectomy, Segmental ; Pneumonectomy ; Pneumonia ; Sputum ; Thoracic Surgery