1.Cryopreservation of Cord Blood Mononuclear Cells in using Programmed Freezer.
Korean Journal of Pediatric Hematology-Oncology 1997;4(2):342-349
No abstract available.
Cryopreservation*
;
Fetal Blood*
2.Effect of peripheral blood cell counts during remission induction and maintenance therapy on the prognosis and therapy of childhood acute lymphoblastic leukemia.
Jun Hee KIM ; Dong Hoon KO ; Dae Keun MOON ; Hoon KOOK ; Tai Ju HWANG
Korean Journal of Hematology 1993;28(1):81-88
No abstract available.
Blood Cell Count*
;
Blood Cells*
;
Precursor Cell Lymphoblastic Leukemia-Lymphoma*
;
Prognosis*
;
Remission Induction*
3.Changes of sexual behavior after radical hysterectomy.
Yong Hoon CHEE ; Won LEE ; Jae Keun CHUNG ; Dae Hoon KIM ; Do Kang KIM ; Sung Eun NAMKOONG ; Seung Jo KIM
Korean Journal of Obstetrics and Gynecology 1991;34(1):53-62
No abstract available.
Hysterectomy*
;
Sexual Behavior*
4.A comparison of ritodrine hydrochloride(Yutopar@) and nifedipine in the treatment of preterm labor.
Jeong Seung LEE ; Jang Heub KIM ; Do Kang KIM ; Dae Hoon KIM ; Hun Young LEE
Korean Journal of Obstetrics and Gynecology 1991;34(3):340-347
No abstract available.
Female
;
Nifedipine*
;
Obstetric Labor, Premature*
;
Pregnancy
;
Ritodrine*
5.A Case of Adenoid Basal Carcinoma in Uterine Cervix.
Dong Choon PARK ; Jae Hoon KIM ; Hyung Gun LEE ; Dae Young CHUNG ; Jin Woo KIM ; Young Ok LEW ; Dae Hoon KIM ; Sung Eun NAMKOONG
Korean Journal of Obstetrics and Gynecology 1999;42(8):1823-1826
We report a rare case of adenoid basal carcinoma in uterine cervix. The patient was a 43-year-old Korean female. She received neoadjuvant chemotherapy with Quick Cis-VP16 (cisplatin and VP16 with 7-10 days interval), three times and radical hysterectomy with pelvic lymph nodes dissection under the diagnosis of cervical cancer stage IIa. After the neoadjuvant chemotherapy, the mass size was 3 x 2.5 Cm and which was protruded in exocervical region. Microscopically, scattered small nests of uniformed small cells with dark nuclei and scant cytoplasm were observed. Peripheral palisading as well as the formation of gland-like or acinar structures were noted. There were also foci of squamous differentiation in same portion of the small nests. The epithelial surface in other portions showed squamous cell carcinoma, large cell non-keratinizing cell type. Distribution between adenoid basal carcinoma of the cervix and other disease, such as adenoid cystic carcinoma and squamous cell carcinoma with basaloid features, is important for clinical management because the clinical behavior of adenoid basal carcinoma is less malignant than adenoid cystic carcinoma.
Adenoids*
;
Adult
;
Carcinoma, Adenoid Cystic
;
Carcinoma, Squamous Cell
;
Cervix Uteri*
;
Cytoplasm
;
Diagnosis
;
Drug Therapy
;
Etoposide
;
Female
;
Humans
;
Hysterectomy
;
Lymph Nodes
;
Uterine Cervical Neoplasms
6.Experimental animal model for preeclampsia by the infusion of nitric oxide synthase inhibitor and reverse of preeclampsia by high dose L-arginine infusion.
Hyung Gun LEE ; Jong Kun LEE ; Young Ok LEW ; Dae Young CHUNG ; Dong Choon PARK ; Jae Hoon KIM ; Jong Chul SHIN ; Dae Hoon KIM ; Soo Pyung KIM
Korean Journal of Obstetrics and Gynecology 1999;42(12):2768-2775
OBJECTIVES: To learn which inhibition of nitric oxide synthase with L-nitro arginine methylester(L-NAME) induces a preeclampsia-like syndrome in pregnant rabbits and high dose of L-arginine reverse the adverse changes induced by nitric oxide synthesis inhibition in pregnant rabbits. MTERIAL AND METHOD: Twenty Newzealand rabbits with 22-days of gestation were injected subcutaneously with 400mg of L-NAME for 7days and 100mg/kg L-arginine was also given intravenously 10 of 20 L-NAME injected pregnant rabbits. They are compared with the control group in which same volume of saline was subcutaneously injected to 5 rabbits with same condition. They were anesthesized by ketamine 50 mg/kg and roupum 2 mg/kg intramuscularly. Cutdown of femoral artery was performed and 22 gauge angioneedle was inserted. On manometer,three way catheter was connected, zeroed with saline, and blood pressure was read. Blood samples were taken from the vein of ear and checked for count of blood cells and bood chemistry (BUN/Cr, GOT/GPT, LDH, Uric acid). Urine protein was measured with nelaton catheterized urine. We injected drugs for 7 days begining on 22 days after mating and performed cesarian section to deliver fetus. To observe their effects on organs, lung, liver, placenta and kidney were taken and fixed with formalin. The sliced kidney tissue in thickness of 1 mm, was fixed with glutaraldehyde for electron microscopy and stored at 4degree C. Special staining was done for closed observation of pattern changes. For statistical analysis, mean+/-SEM was used. The control and experimental groups were compared by unpaired t-test and the differences were significant if probability level is less than 0.05(<0.05). RESULT: Mean blood pressure(MAP) in the experimental group I was significantly high compared to the control group(P<0.05). There was no significant differences in MAP between experimental group II and control group. Urine Protein, BUN, Cr, GOT/GPT, LDH, platelet count in the experimental group I was significantly high(p<0.05) compared to the control group. There was no significant difference between experimental group II and control group. In light microscopic examination, lung, liver, kidney, placenta showed specific finding in experimental group I. Misconstructive of glomerulus in the experimental kidney was well preserved under EM examination. Interstitium of kidney was widened by increase of mesangial matrix. Mild effacement of foot process and cytoplasm of proximal tubule containing electron dense myelin figure like structure were observed. CONCLUSION: Long term injection of L-arginine analogue produced preeclampsia like syndrome and pathologic changes of organ system in pregnant rabbits. Concurrent high dose of L-arginine reversed such chages.
Arginine*
;
Blood Cells
;
Blood Pressure
;
Catheters
;
Chemistry
;
Cytoplasm
;
Ear
;
Femoral Artery
;
Fetus
;
Foot
;
Formaldehyde
;
Glutaral
;
Ketamine
;
Kidney
;
Liver
;
Lung
;
Microscopy, Electron
;
Models, Animal*
;
Myelin Sheath
;
NG-Nitroarginine Methyl Ester
;
Nitric Oxide Synthase*
;
Nitric Oxide*
;
Placenta
;
Platelet Count
;
Pre-Eclampsia*
;
Pregnancy
;
Rabbits
;
Veins
7.A Case of Ant Sting by Crematogaster Matsumurai Vagala.
Jung Dae KANG ; So Jin KIM ; Nyoung Hoon YOUN ; Boung Jin KIM ; Seok Don PARK
Annals of Dermatology 2004;16(2):87-89
Reactions from stinging insects are significant to dermatologists because of the wide variety of clinical presentations from mild local reaction to severe anaphylactic reaction. Although ant sting commonly occurs, it has been rarely reported in the literatures. We experienced a unique case with allergic reactions by the ant of the Cremawgaster iw6w urai vagala, subfamily Myrmicinae, family Formicidea, order Hymenoptera that has not been previously reported.
Anaphylaxis
;
Ants*
;
Bites and Stings*
;
Humans
;
Hymenoptera
;
Hypersensitivity
;
Insects
8.Determination of antigenic domain in GST fused major surface protein (Nc-p43) of Neospora caninum.
Eui Sun SON ; Hye Jin AHN ; Jae Hoon KIM ; Dae Yong KIM ; Ho Woo NAM
The Korean Journal of Parasitology 2001;39(3):241-246
The antigenic domain of the major surface protein (Nc-p43) of Neospora caninum was examined by polymerase chain reaction of its gene fragments and recombinant expression as GST fusion proteins. The fragments of Nc-p43 were as follow: a total open reading frame (OFR), T; OFR without signal sequence and C-terminal hydrophobic sequence, S; N-terminal 2/3 parts of S, A; C-terminal 2/3 parts, P; N-terminal 1/3 part, X; middle 1/3 part, Y; and C-terminal 1/3 part, Z, respectively. The DNA fragments were cloned into pGEX-4T vector. Recombinant plasmids transformed into Escherichia coli of BL21 pLysS (DE3) strain were induced to express GST or GST fused fragments of Nc-p43 such as 69 kDa protein for T, 66 kDa for S, 52 kDa for A, 53 kDa for P, and 40 kDa proteins for X, Y, and Z, respectively in SDS-PAGE. The Nc-p43 fragments of T, S, and P reacted with a bovine serum of neosporosis while those of A, X, Y, and Z together with GST did not in the western blot. These findings suggest that the antigenic domain of Nc-p43 of N. caninum may be localized in the C-terminal 2/3 parts. Together with A19 clone in SAG1 of Toxoplasma gondii (Nam et al., 1996), the P fragment of Nc-p43 could be used as efficient antigens to diagnose and differentiate those infections with both species.
Amino Acid Sequence
;
Animals
;
Base Sequence
;
Biological Markers/analysis
;
Blotting, Western
;
Cells, Cultured
;
Cercopithecus aethiops
;
Coccidiosis/diagnosis
;
Neospora/*immunology
;
Protozoan Proteins/*analysis/genetics/immunology
;
Reverse Transcriptase Polymerase Chain Reaction
;
Support, Non-U.S. Gov't
;
Vero Cells/parasitology
9.Experimental microarterial grafts: glutaraldehyde-tanned microvascular heterografts versus autografts.
Dong Hoon WOO ; Dae Sup KIM ; Chin Ho YOON ; Han Joong KIM ; Hong Mee YOUCK
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1992;19(3):359-364
No abstract available.
Autografts*
;
Heterografts*
;
Transplants*
10.Usefulness of Treadmill Exercise Test on Diabetes Mellitus.
Tae Hoon LEE ; Yoon Ho LEE ; Seung Tae CHUNG ; Eung Jin KIM ; Dae Ha KIM
Korean Circulation Journal 1985;15(2):215-223
We studied 193 Diabetics without any symptoms of coronary artery disease and 39 Normal healthy subjects as Control group, using treadmill exercise test and obtained following results. 1) The positive rate was significantly higher in Diabetics(19.4% in Normal ECG groups and 25.4% in total Diabetics)than Control groups(5.1%)(0.01
0.05). 5) There was no difference in HbAIC, Total cholesterol and Triglyceride between positive and negative groups(P>0.05). In summary, with exercise test, the positive rate was higher in Diabetics than in Normal control groups and it seemed to be treadmill exercise test is a useful screening method for evaluation of coronary artery disease as well as for assesment of treatment and follow up care.
Blood Pressure
;
Cholesterol
;
Coronary Artery Disease
;
Diabetes Mellitus*
;
Electrocardiography
;
Equidae
;
Exercise Test*
;
Heart Rate
;
Mass Screening
;
Triglycerides