1.The Effects of Glutamate Receptor Antagonists on Cultured Cerebral Cortical Neurons of Neonatal Mouse Damaged by Oxidative Stress.
Dae Ho CHOI ; Yeon Kyun OH ; Seung Taek PARK
Journal of the Korean Pediatric Society 1999;42(8):1096-1103
PURPOSE: To evaluate neurotoxic effects induced by oxygen-radicals, which were generated by adding xanthine oxidase(XO) and hypoxanthine(HX), and protective effects of glutamate receptor antagonist such as MK-801 and 6-cyano-7-nitroquinoxaline(CNQX). METHODS: Dissociated cell cultures were prepared from cerebrum of neonatal mouse. Tissues were dissected and diced into small pieces in phosphate buffered saline and were incubated at 37degrees C. Isolated cells were resuspended in Eagle's minimum essential medium and plated poly-L-lysine coated plastic coverslips in 96 well multichambers at a cell density of 3x105 cells/well. Cells were grown in a 5% CO2/95% air atmosphere at 37degrees C. Cytotoxic effects were examined in cerebral cortical neurons cultured for 3 hours in media containing various concentration of XO and HX. The protective effects of glutamate receptor antagonist were also examined by MTT assay and neurofilament enzymeimmunoassay(EIA). Microscopic examinations were also done. RESULTS: Oxygen radicals markedly induced decrement of the cell viability of cultured mouse cerebral cortical neurons in a dose-dependent manner. Midpoint cytotoxicity value was 30mU/ml XO/0.1mM HX, when mouse cerebral cortical neurons were incubated for 3 hours with various concentrations of XO and HX. The number of cells and neurites was decreased when cerebral cortical neurons were cultured for 3 hours in a medium containing 30mU/ml XO/0.1mM HX. MK- 801 was very effective in blocking oxidant-induced neurotoxicity, while CNQX falied to show any protective effect in these cultures. CONCLUSION: It is suggested that oxygen radicals are neurotoxic, and selective N-methyl-D-aspartate antagonists such as MK-801 are very effective in protecting neurotoxicity induced by oxygen radicals in cultured cerebral cortical neurons of neonatal mouse.
6-Cyano-7-nitroquinoxaline-2,3-dione
;
Animals
;
Anoxia
;
Atmosphere
;
Cell Count
;
Cell Culture Techniques
;
Cell Survival
;
Cerebrum
;
Dizocilpine Maleate
;
Excitatory Amino Acid Antagonists*
;
Glutamic Acid*
;
Ischemia
;
Mice*
;
N-Methylaspartate
;
Neurites
;
Neurons*
;
Oxidative Stress*
;
Plastics
;
Reactive Oxygen Species
;
Receptors, Glutamate*
;
Xanthine
2.A morphological study on residual alveolar ridges of edentulous jaws .
Ho Young CHOI ; Yi Hyung WOO ; Dae Gyun CHOI
The Journal of Korean Academy of Prosthodontics 1991;29(1):73-89
No abstract available.
Jaw, Edentulous*
3.Comparative study of STIR image with SE image in diagnosis of the orbital disease.
Soo Nami CHOI ; Ho Kyu LEE ; Dae Chul SUH
Journal of the Korean Radiological Society 1993;29(6):1140-1145
To evaluate the efficacy of STIR (short tau inversio recovery) image in the diagnosis of orbital diseases, we compared the STIR images with conventional SE(spin-echo) images in 17 patients. The fat signal was reliably nulled at inversion time of approximately 160-170 msec in all cases. We analyzed all the cases in the aspects of the detection of lesion and the lesion-to-back ground contrast retrospectively. Regarding lesion, the detection rates of STIR, T1 and T2-weighted images were 89, 88 and 83% respectively. In the aspect of lesion-to-background contrast, the contrast ratios of STIR and T1, T2-weighted image were 87% and 64, 56%, respectively. In conclusion, STIR images were similar to T1-and T2-weighted images in the detection of lesion, but superior in the aspect of lesion-to-background contrast. So the STIR images may be a useful adjunct to conventional SE images for the diagnosis of orbital disease.
Diagnosis*
;
Humans
;
Orbit*
;
Orbital Diseases*
;
Retrospective Studies
4.Atrial myxoma (a report of 5 cases).
Yong Dae CHOI ; Min Ho KIM ; Kong Soo KIM
The Korean Journal of Thoracic and Cardiovascular Surgery 1992;25(7):756-762
No abstract available.
Myxoma*
5.The long term follow-up study for insall-burstein posterior stabiliazed knee.
Dae Kyung BAE ; Yong Girl RHEE ; Ho CHOI
The Journal of the Korean Orthopaedic Association 1991;26(3):863-871
No abstract available.
Follow-Up Studies*
;
Knee*
6.Results of total knee replacement in old infection sequale.
Dae Kyung BAE ; Ho CHOI ; Hyung Koo KIM
The Journal of the Korean Orthopaedic Association 1992;27(1):25-33
No abstract available.
Arthroplasty, Replacement, Knee*
7.Fractures of the tibial intercondylar eminence.
Jin Whan AHN ; Dae Kyung BAE ; Ho CHOI
The Journal of the Korean Orthopaedic Association 1991;26(4):1101-1106
No abstract available.
8.Erratum: Author Correction.
So Mi CHOI ; Min Youp CHOI ; Woo Dae KANG ; Ho Sun CHOI ; Seok Mo KIM
Obstetrics & Gynecology Science 2014;57(5):424-424
The author list should be corrected.
9.Endoprosthesis in the Hip Joint
Se Il SUK ; Dae Kyung BAE ; In Ho CHOI ; Myung Ho KIM
The Journal of the Korean Orthopaedic Association 1978;13(2):95-103
Endoprosthetic replacement was performed in 38 patients at the department of orthopedic surgery, Seoul National University Hospital in the past ten years from May, 1968, and following results were obtained. 1. Among 38 patients, fracture was in 33 patients, avascular necrosis in 3,and osteoarthritis in 2. 2. Austin-M oore prosthesis was used in 31 patients and Thompson prosthesis in 7. 3. The youngest age was 34 years old and the oldest 85, and the peak incidence was in 7th decade in male and 8th decade in female. 4. The most common type of femur neck fracture was transcervical (60.6%) followed with basal neck (21.1%) and subcapitel (18.2%) 5. Of 33 cases of femur neck fracture, 21 patients (63.6%) was admitted more than 3 weeks after injury. 6. Moore type Southern approach was used most frequently (55.2%). 7. In 36 patients (94.6%), early weight bearing was permitted within 3 weeks, and 25 patients (65.7%) were discharged within 2 weeks after surgery. 8. Minor complications were encountered in 12 patients (31.5%), and none were severe. 9. In follow up of more than 3 months, good to excellent results were obtained in 75%.
Female
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Femoral Neck Fractures
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Follow-Up Studies
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Hip Joint
;
Hip
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Humans
;
Incidence
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Male
;
Neck
;
Necrosis
;
Orthopedics
;
Osteoarthritis
;
Prostheses and Implants
;
Seoul
;
Weight-Bearing
10.A Case of Surgically Corrected-Combined form of Total Anomalous Pulmonary Venous Return.
Dae Sung HWANG ; Sun Hwan CHO ; Jong Bum CHOI ; Hyang Suk YOON ; Soon Ho CHOI
Korean Circulation Journal 1992;22(3):473-478
Total anomalous pulmonary venous return(connection) is a congenital heart disease. Especially, the reports of the patients with double connections of all pulmonary veins were rare. An infant who underwent operation for total anomalous pulmonary venous return had double drainge of all pulmonary veins to both left vertical vein and anomalous descending vein. This second lesion was not diagnosed preoperatively, but in operative field. Operative treatments were performed by ligation of left vertical vein and central pulmonary vein-to-left atrium anastomosis without ligation of the descending anomalous vein. For these less common variants fo total anomalous pulmonary venous return,a more detailed and precise understanding of morphology can facilitate accurate surgical repair. And also, early detection of the disease is an another issue for a good prognosis.
Heart Defects, Congenital
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Humans
;
Infant
;
Ligation
;
Prognosis
;
Pulmonary Veins
;
Scimitar Syndrome*
;
Veins