Objective:To investigate the protective effect of all-trans retinoic acid(ATRA)on the function of NK cells dam-aged by oxidative stress in liver fibrosis.Methods:Mouse model with liver injury was established by carbon tetrachloride(CCl4),biochemical and pathological assays were used to evaluate the degree of liver injury and fibrosis in mice,and changes of NK cells and oxidative stress injury in liver tissue of mice were observed.In vitro,NK92 cell lines damaged by H2O2 oxidative stress were evaluated the protective effect of ATRA.Results:Liver inflammation and fibrosis were suppressed and liver function was improved in CCl4 model mice by ATRA treatment.ATRA could increase SOD activity and GSH content in liver tissue,which promoted the proportion and num-ber of hepatic NK cells.ATRA could protect NK cells from oxidative damage and apoptosis induced by H2O2 in vitro.Conclusion:ATRA can ameliorate liver injury and fibrosis induced by CCl4,which may be related to the inhibition of oxidative stress on NK cells.

Objective To investigate the mechanism of Fuzheng Huayu(FZHY) decoction in the treatment of liver cancer based on network pharmacology. Methods TCMSP, BATMAN, and Drugbank databases were searched for the main chemical components and corresponding targets of FZHY, and STRING database was used to perform a PPI network analysis. Cytoscape software was used to establish a drug-disease network model and perform a network analysis, and R language was used to perform GO and KEGG enrichment analysis of targets. Results A total of 192 intersection genes between FZHY and liver cancer and 95 potential compounds were screened out, among which quercetin and luteolin were the active components with an important regulatory role. INS, IL-6, and EGFR were the key targets for the potential effect of FZHY. The GO enrichment analysis showed the involvement in various biological processes such as response to drug and response to oxygen level, and the KEGG enrichment analysis showed the involvement in the signaling pathways including apoptosis and tumor necrosis factor signaling pathways. Conclusion Based on the method of network pharmacology, this study reveals the mechanism of action of multiple targets and targets of FZHY in the treatment of liver cancer, which provides a theoretical basis for clinical and basic scientific research.