OBJECTIVE:To compare the different sample preparation and optimize the isolation of three kinds of alkaloids in Aconitum carmichaeli.METHODS:RP-HPLC was applied and performed on Zorbax extend-C18 column (150 mm?4.6 mm,5 ?m) column.The differences among the content of alkaloids prepared by preparation technologies were compared.RESULTS:The best preparation was aether ultrasonic extraction.The separation condition was as follows:mobile phase consisted of acetonitrile-0.1% ethylenediamine (gradient elution) with flow rate of 1 mL?min-1.The detection wavelength was set at 240 nm and column temperature was 30 ℃.CONCLUSION:Aether ultrasonic extraction is simple with ideal separation,extraction and re-covery to provide scientific basis for the quality control of A.carmichaeli.

OBJECTIVE: To research the content of alkaloids and polysaccharides in different varieties of Aconitum carmichaeli. METHODS: The contents of total alkaloids, diester diterpenoid alkaloids and polysaccharides were measured by acid dye colorimetry, HPLC and sulphuric acid-anthrone colorimetric method respectively. RESULTS: In different varieties, the differences of total alkaloids and diester diterpenoid alkaloids were significant. However, the difference of polysaccharides wasn’t significant. The content of total alkaloids and diester diterpenoid alkaloids in processed A. carmichaeli reduced significantly while the content of polysaccharides increased slightly. CONCLUSION: The results of study could provide a scientific basis for rational breeding of A. carmichaeli.