1.Abnormal semen liquefaction and seminal plasma lipoprotein (a).
Fu-Xin LIU ; Da-Lin SU ; Guo-Yong ZHU
National Journal of Andrology 2013;19(3):247-250
OBJECTIVETo explore the effect of seminal plasma lipoprotein (a) in abnormal semen liquefaction and its clinical significance.
METHODSAccording to The WHO Laboratory Manual for the Examination and Processing of Human Semen, we conducted semen routine analyses of 101 patients with abnormal semen liquefaction and 26 normal healthy controls. We added chymotrypsin to the semen for 30 minutes of incubation at 37 degrees C. When there were filaments, we centrifuged the semen and obtained the upper seminal plasma to determine the level of lipoprotein (a).
RESULTSThe level of lipoprotein (a) was significantly higher in the 101 patients ([526.2 +/- 243.5] mg/L) than in the 26 normal controls ([296.9 +/- 105.2] mg/L) (P < 0.01) .
CONCLUSIONLipoprotein (a) can inhibit fibrin dissolution, and delayed fibrin dissolution in semen liquefaction may be related to the increased level of seminal plasma lipoprotein (a). The seminal plasma lipoprotein (a) level should be taken into account in the clinical diagnosis of male infertility caused by abnormal semen liquefaction.
Adult ; Case-Control Studies ; Humans ; Infertility, Male ; metabolism ; physiopathology ; Lipoprotein(a) ; analysis ; Male ; Semen ; metabolism ; Seminal Plasma Proteins ; analysis ; Young Adult
2.GVHD Following Autologous Peripheral Blood Stem Cell Trasplantation Reduced Malignancy Relapse
Da-Si GUO ; Xiao-Lin LI ; Su ZHANG ; Jie FU ; Yang YANG ; Yue YIN
Journal of Experimental Hematology 2001;9(3):287-288
Administration of the immunosuppressive drug cyclosporine (CSA) after autologous peripheral blood stem cell transplantation (APBSCT) induces a systemic auto-immune syndrome resembling graft-versus-host disease (GVHD), this syndrome termed autologous GVHD has significant antitumor activity, it can reduce the incidence of tumor relapse after APBSCT. The antitumor effect of this auto-aggression syndrome can be enhanced by the administration of gamma-interferon (gamma-IFN). Five consecutive patients who received APBSCT received therapy inducing autologous GVHD. Intravenous administration of CSA [1 mg/(kg.d) for 28 days] was begin on the day of transplantation. gamma-interferon (0.025 mg/m(2) qod) was administered sub-cutaneously from days 7 throngh 28 after transplatation. Results showed that four of five occured autologous GVHD-skin demage, five in control didn't occur autologous GVHD. The relapse rate of the treated cases was 20% (1/5) versus 60% (3/5) of the control, and the median survival time of the treated cases was 20 (4 - 30) months versus 10 (2 - 20) months of the control. The data indicates that autologous GVHD results in low relapse rate of the patients rececving APBSCT.
3.Develop a statistics analysis software in population genetics using VBA language.
Ying CAI ; Ni ZHOU ; Ye-li XU ; Da-peng XIANG ; Jiang-hui SU ; Lin-tian ZHANG
Journal of Forensic Medicine 2006;22(6):417-420
OBJECTIVE:
To develop a statistics analysis software that can be used in STR population genetics for the purpose of promoting and fastening the basic research of STR population genetics.
METHODS:
Selecting the Microsoft VBA for Excel, which is simple and easy to use, as the program language and using its macro function to develop a statistics analysis software used in STR population genetics.
RESULTS:
The software "Easy STR Genetics" based on VBA language, by which the population genetic analysis of STR data can be made, were developed.
CONCLUSION
The developed software "Easy STR Genetics" based on VBA language, can be spread in the domain of STR population genetics research domestically and internationally, due to its feature of full function, good compatibility for different formats of input data, distinct and easy to understand outputs for statistics and calculation results.
Algorithms
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Electronic Data Processing
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Genetics, Population/statistics & numerical data*
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Humans
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Microsatellite Repeats
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Quality Control
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Software
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Software Design
4.Relationship between apolipoprotein E and apolipoprotein B polymorphisms in youths with coronary heart disease.
Sha LI ; Zhao-wen LEI ; Zili CHEN ; Da LIN ; Xi-song KE ; Yao-ming ZHONG ; Su-fen WU
Chinese Journal of Medical Genetics 2003;20(3):241-243
OBJECTIVETo investigate hereditary susceptibility to coronary heart disease (CHD) in apolipoprotein E(apo E) and apo B polymorphisms of youths.
METHODSPolymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to analyze apoE, apoB Xba I, apoB 3' variable number of tandem repeat (VNTR) genotypes for 244 healthy Han students (among them were 109 students with positive CHD family history).
RESULTSThe allele frequencies of apo e4, XbaI x(+), 3'VNTR-B(hypervariable element, HVE>38) in the positive group were obviously higher than those in the negative group(P<0.05), and were significantly correlated with the increase in TC, LDL-C, apoB100 levels (P<0.05).
CONCLUSIONThe alleles for apo e4, XbaI x(+), 3'VNTR-B may be the important genetic markers of Han CHD.
Adolescent ; Alleles ; Apolipoproteins B ; genetics ; Apolipoproteins E ; genetics ; Coronary Disease ; genetics ; Female ; Gene Frequency ; Humans ; Male ; Polymerase Chain Reaction ; Polymorphism, Genetic ; Young Adult
5.Early Osseointegration of Implants with Cortex-like TiO2 Coatings Formed by Micro-arc Oxidation: A Histomorphometric Study in Rabbits
LI YA-DA ; LIU LIN ; CHEN XIAO-DONG ; WANG WEI-QIANG ; MA GUO-WU ; SU YU-CHENG ; QI MIN
Journal of Huazhong University of Science and Technology (Medical Sciences) 2017;37(1):122-130
In our previous studies,a novel cortex-like TiO2 coating was prepared on Ti surface through micro-arc oxidation (MAO) by using sodium tetraborate as electrolyte,and the effects of the coating on cell attachment were testified.This study aimed to investigate the effects of this cortex-like MAO coating on osseointegration.A sand-blasting and acid-etching (SLA) coating that has been widely used in clinical practice served as control.Topographical and chemical characterizations were conducted by scanning electron microscopy,energy dispersive X-ray spectrometer,X-ray diffraction,contact angle meter,and step profiler.Results showed that the cortex-like coating had microslots and nanopores and it was superhydrophilic,whereas the SLA surface was hydrophobic.The roughness of MAO was similar to that of SLA.The MAO and SLA implants were implanted into the femoral condyles of New Zealand rabbits to evaluate their in-vivo performance through micro-CT,histological analysis,and fluorescent labeling at the bone-implant interface four weeks after surgery.The micro-CT showed that the bone volume ratio and mean trabecular thickness were similar between MAO and SLA groups four weeks after implantation.Histological analysis and fluorescent labeling showed no significant differences in the bone-implant contact between the MAO and SLA surfaces.It was suggested that with micro/nanostructure and superhydrophilicity,the cortex-like MAO coating causes excellent osseointegration,holding a promise of an application to implant modification.
6.GLI-1 is involved in EGF-regulated enhancement of the invasiveness of prostate cancer ARCaP(E) cells in vitro.
Guo-dong ZHU ; Jian-cheng ZHOU ; Jin ZENG ; Zhen-kun MA ; Bo-xing SU ; Xin-yang WANG ; Da-lin HE
National Journal of Andrology 2012;18(1):16-22
OBJECTIVETo investigate the role of the hedgehog (HH) signaling pathway transcription factor glioma-associated oncogene hoinolog 1 (GLI-1) in EGF-regulated enhancement of the invasiveness of the prostate cancer ARCaP(E) cell line in vitro.
METHODSThe expressions of EGFR and GLI-1 in prostate cancer ARCaP(E) cells were analyzed by immunofluorescence staining. ARCaP(E) cells were treated with EGF at 100 ng/ml, followed by detection of the changes in cell morphology and invasiveness, as well as in the expressions of p-ERK, ERK and GLI-1. Migration transwell assay was used to determine the effects of 100 ng/ml EGF and GLI-1 antagonist GANT61 on the invasiveness of the ARCaP(E) cells.
RESULTSBoth EGFR and GLI-1 were expressed in the ARCaP(E) cells. EGF induced morphological transition of epithelial-like ARCaP(E) cells to mesenchymal-like cells, increased their in vitro invasiveness, and significantly upregulated the expressions of p-ERK and GLI-1 in the ARCaP(E) cells (P<0.05). GANT61 significantly inhibited the in vitro invasiveness of the ARCaP(E) cells and reduced the enhancing effect of EGF on their invasiveness (P<0.05).
CONCLUSIONThe results from ARCaP(E) cells shed light on the cross-talk of the HH pathway with the EGF/ERK signaling pathway. GLI-1 might be responsible for EGF-regulated enhancement of the invasiveness of ARCaP(E) cells in vitro.
Cell Line, Tumor ; Epidermal Growth Factor ; metabolism ; Humans ; Male ; Prostatic Neoplasms ; metabolism ; pathology ; Signal Transduction ; Transcription Factors ; genetics ; metabolism ; Zinc Finger Protein GLI1
7.Pith decompression of the femoral head and allograft fibula grafting for treatment of avascular necrosis of femoral head.
Zhi-jiong LIN ; Pei-ji SU ; Zhong-qing WU ; Da-wei GAO ; Zhao-qing LI ; Jian-song YANG
China Journal of Orthopaedics and Traumatology 2009;22(8):628-630
OBJECTIVETo investigate the clinical efficacy of the pith decompression of the femoral head and fibular allograft transplantation in the treatment of early stage avascular necrosis.
METHODSFrom January 2004 to November 2008, 32 hips of 25 patients with avascular necrosis of femoral head of Ia-IIIb period were treated by the pith decompression of the femoral head and fibular allograft transplantation, hollow lag screw fixation, included 17 males and 8 females, aged from 20 to 55 years old (39.1 years on average). Preoperative pain was from 2 to 14 months (means 5.5 months). All patients were applied on conventional X-ray films, MRI examination, Harris score.
RESULTSThe patients were followed up for 12 to 48 months (means 36.4 months). X-ray film showed 21 hips of 18 cases improved,6 hips of 4 cases unchanged, no collapse of articular surface, 3 hips of 2 cases deterioration, 2 hips of 1 case failed. Preoperative Harris score was (77.0 +/- 8.0) and postoperative (90.6 +/- 2.5), there was a significant difference (t = 1.67, P < 0.05).
CONCLUSIONPith decompression of the femoral head and fibular allograft transplantation in the treatment of early stage avascular necrosis of femoral head has advantage of joint function in bed-ridden after a short time, quick recovery,clinical symptoms improved. Its short-term efficacy is certain but long-term efficacy is still need further observation.
Adult ; Decompression, Surgical ; methods ; Female ; Femur Head Necrosis ; surgery ; Fibula ; transplantation ; Humans ; Male ; Middle Aged ; Transplantation, Homologous
8.Identification of the metabolites of Sinisan extract in rat plasma, urine, feces and bile after intragastric administration.
Lin-ling LU ; Yan SHU ; Da-wei QIAN ; Shu-lan SU ; Jin-ao DUAN ; Ye-fei QIAN ; Cai-fu XUE
Acta Pharmaceutica Sinica 2011;46(11):1374-1379
Sinisan is a widely used traditional Chinese medicine (TCM) in treating various diseases; however, the in vivo metabolic profile of its multiple components remains unknown. In this paper, ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) was applied to identify the metabolites of Sinisan extract in rat plasma, urine, feces and bile after intragastric administration. Using MS(E) and mass defect filter techniques, 41 metabolites of 10 parent compounds (naringin, naringenin, hesperidin, neohesperidin, liquiritin, liquiritigenin, glycyrrhizic acid, glycyrrhetinic acid, saikosaponin a and saikosaponin d) were detected and tentatively identified. It was shown by our results that these compounds was metabolized to the forms of hydroxylation, glucuronidation, sulfation, glucuronidation with sulfation and glucuronidation with hydroxylation in vivo.
Administration, Oral
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Animals
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Chromatography, High Pressure Liquid
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Drugs, Chinese Herbal
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metabolism
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pharmacokinetics
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Flavanones
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analysis
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metabolism
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pharmacokinetics
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Glucosides
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analysis
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metabolism
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pharmacokinetics
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Glycyrrhizic Acid
;
analysis
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metabolism
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pharmacokinetics
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Hesperidin
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analogs & derivatives
;
analysis
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metabolism
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pharmacokinetics
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Hydroxylation
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Male
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Plants, Medicinal
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chemistry
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Random Allocation
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Rats
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Rats, Sprague-Dawley
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
9.Clinical observation of 100 patients with malignant lymphoma treating with different preconditioning regimens followed by autologous hematopoietic stem cell transplantation.
Lan-Lan SHAO ; Xiu-Bin XIAO ; Kai-Li ZHONG ; Yun LU ; Xi-Lin CHEN ; Yong DA ; Jing LIU ; Shi-Hua ZHAO ; Yi MA ; Qiu-Shi YANG ; Hang SU ; Wei-Jing ZHANG
Journal of Experimental Hematology 2012;20(3):598-602
This study was designed to compare the curative effect, prognosis and safety of different preconditioning regimens for patients who received autologous hematopoietic stem cell transplantation (AHSCT) for malignant lymphoma (ML). The clinical data of 100 ML patients (Sep 1992 to Aug 2010 in 307 Hospital) were retrospectively analyzed, and were divided into two groups by different preconditioning regimens: the high-dose chemotherapy preconditioning group and high-dose chemotherapy/radiotherapy preconditioning group. The overall survival (OS) rate, progress free survival (PFS) rate and adverse effect were analyzed. The results showed that until Feb 2011, the median follow-up was 33.5 months. All patients were engrafted and their hematopoiesis was reconstituted. The median time of WBC recovery up to > 1.0×1.0(9)/L in high-dose chemotherapy preconditioning group and high-dose chemotherapy/radiotherapy preconditioning group were (6.0 ± 0.4) d and (8.2 ± 0.4) d, platelet up to > 20.0×1.0(9)/L in two groups were (7.1 ± 0.8) d and (11.4 ± 2.5) d (P < 0.05). The 3-year OS rate of the two groups were 67.3% and 68.9%. 5-year OS rates of two groups were 62.8% and 60.6%, 10-year OS rates of two groups were 57.6% and 56.2% respectively; 3-year PFS of two group were 63.6% and 63.2%, 5-year of two group were 59.4% and 58.3%, 10-year of two group were 50.8% and 55.3% respectively (P > 0.05). Meanwhile, the incidence of fever, infection, bleeding, secondary cancer between two groups was not significant different (P > 0.05). It is concluded that the hematopoietic reconstitution of high-dose chemotherapy/radiotherapy preconditioning group is later than that of high-dose chemotherapy preconditioning group. However, there is no significant difference in curative effect and prognosis between the two groups.
Adolescent
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Adult
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Aged
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Child
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Combined Modality Therapy
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Female
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Hematopoietic Stem Cell Transplantation
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Humans
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Lymphoma
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surgery
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therapy
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Male
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Middle Aged
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Prognosis
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Retrospective Studies
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Transplantation Conditioning
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methods
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Transplantation, Autologous
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Young Adult
10.Relationship between the changes in immune cells and HBeAg loss following antiviral treatment in chronic hepatitis B patients.
Qi ZHENG ; Yue-yong ZHU ; Jing CHEN ; Yu-rui LIU ; Jia YOU ; Da-wu ZENG ; Su LIN ; Jia-ji JIANG
Chinese Journal of Hepatology 2012;20(11):801-806
OBJECTIVETo observe the changes in hepatitis B virus (HBV)-specific and non-specific cellular immunity that accompany viral load decline during adefovir dipivoxil (ADV) treatment in patients with hepatitis B e antigen (HBeAg)-positive chronic hepatitis B, and to explore the antiviral immunity mechanism underlying the treatment response.
METHODSSerial analysis of cellular immunological parameters was performed in HBeAg-positive patients (n = 20) throughout the 48-week course of ADV therapy (10 mg/d). HBV-specific T cell reactivity to HBV core antigen (HBcAg) was assessed by enzyme-linked immunosorbent spot assay and cell proliferation assay at pre-treatment (baseline) and post-treatment weeks 4, 12, 24, 36, and 48. Percentage of regulatory T cells (Tregs), as well as activated peripheral natural killer (NK) cells (expressing the NKG2D receptor), was measured by flow cytometry. Comparisons of means were performed by the two-tailed t-test or the Mann-Whitney rank sum test.
RESULTSAfter 48 weeks of ADV therapy, HBeAg loss was observed in six of the 20 (30%) patients and 14 patients remained HBeAg-positive. In the patients with HBeAg loss, the viral load reduction was accompanied by a significantly enhanced response rate of HBV-specific interferon (IFN)-gamma-producing CD4+ T cells [measured as (spot forming cells/peripheral blood mononuclear cells); baseline: (661.25+/-281.97) *10(-6) vs. week 48: (280.75+/-104.33) *10(-6), P = 0.045]. In contrast, patients without HBeAg loss showed no significant differences in T cell response rates. The patient groups with and without HBeAg loss showed similar proportions of peripheral blood Tregs during the treatment course, which included a trend of gradual decrease from baseline to week 4 with steady levels thereafter. In addition, both groups showed a similar increase in NKG2D expression that began at week 12 and peaked at week 48.
CONCLUSIONHBV-specific T cell reactivity temporally increases in some ADV-treated chronic hepatitis B patients, and this trend is strongly associated with HBeAg loss. Furthermore, recovery of HBV-specific T cell reactivity promotes viral clearance and HBeAg seroconversion.
Adult ; Antiviral Agents ; therapeutic use ; DNA, Viral ; blood ; Female ; Hepatitis B e Antigens ; blood ; Hepatitis B, Chronic ; blood ; drug therapy ; immunology ; Humans ; Killer Cells, Natural ; immunology ; Male ; NK Cell Lectin-Like Receptor Subfamily K ; metabolism ; T-Lymphocytes, Regulatory ; immunology ; Viral Load ; Young Adult