Extramedullary plasmacytoma (EMP) is a rare tumor characterized by proliferation of monoclonal plasma cells,often occurs in the head and neck,followed by gastrointestinal and skin.Diagnosis is based on biopsy,which is the only accurate and reliable method.EMP is needed to discriminate with similar diseases.Radiotherapy is the preferred treatment method of EMP,because it has a higher radiation sensitivity.Sometime we can choose comprehensive treatment because of illness need.Hematopoietic stem cell transplantation is not as the preferred treatment option because of transplant rejection.

Objective To investigate the role of human estrogen receptor-related receptor(ERR) ?,a submember of orphan receptors,in the tumorigenesis of endometrial cancer.Methods Plasmid of pSG-ERR? was transfected into endometrial cancer cell lines HEC-1A,HEC-1B,and Ishikawa.Real-time quantitative RT-PCR and western blot were used to analyze the mRNA and protein expression of ERR? in endometrial cancer cell.Flow cytometry was used to analyze the cellular growth.Results Expressions of the ERR? were significantly increased in the endometrial cancer cells transfected with pSG-ERR? plasmid; expression of the ERR? mRNA in HEC-1A cell was 9644.4 copies/ng,HEC-1B:9835.3 copies/ng,and Ishikawa:8008.6 copies/ng(P

Humans ; Moxibustion ; Depression ; Acupuncture Therapy ; Gastroesophageal Reflux ; Esophagitis, Peptic

Obejective To explore mechanism and inhibition of thalido-mide ( Tha ) combined with epirubicine ( Epi ) on the growth of trans-planted H22 hepatocellular carcinoma in mice.Methods Forty male Kunming mice were transplanted by right armpit injection of 200 μL (2.0 ×106 cells) H22 tumor cells.Then these mice were randomly di-vided into four groups, ten mice in each group.Saline group, Tha group ( oral 200 mg · kg -1 · d-1 for 10 consecutive days ); Epi group ( intra-peritoneall injection, 50 mg · m-2 , once ) , Tha combined with Epi group ( oral Tha 200 mg · kg -1 · d-1 , for 10 consecutive days; Epi intraperitoneall injection, 50 mg· m-2 , once).The tumor diameter and short diameter was measured every 7 d, and twenty -eighth days were sacrificed by dislocation , tumor tissue was obtained.The expression of B-cell lymphoma-2 ( Bcl -2 ) , Bcl -2 associated X protein ( Bax ) , and vascular endothelial growth factor ( VEGF ) mRNA was measured by RT -PCR.The expression of Bax , Bcl -2 protein was measured by Western blot.The expression of VEGF protein was measured by immunohistochemical.Results After 21 days, compared with saline group (0.90 ±0.01), Epi group (0.67 ±0.01), Tha group (0.62 ±0.02) and combined group (0.43 ±0.01) transplanted tumor volume was decreased (P<0.05).Compared with Epi group and Tha group, combined group transplanted tumor volume was decreased (P<0.05).Compared with saline group, the expression of Bcl-2 and VEGF mRNA and protein was down -regulated ( P<0.05 ) , the expression of Bax mRNA and protein was up-regulated in Epi group, Tha group and combined group (all P<0.05).The expression of Bax, Bcl -2 and VEGF mRNA and protein had significant difference in combined group compared with Tha and Epi group (P<0.05). Conclusion Tha and Epi could inhibit the growth of transplanted H22 hepatocellular carcinoma, related to lower VEGF and Bcl-2 expression as well as higher Bax expression , and Tha combined with Epi might have synergistic effect.

Previous studies have found that 40% of patients with acute ST-segment elevation myocardial infarction(STEMI) combine with multiple vascular lesions(MVD), which is known as one of the main risks for poor prognosis in patients with myocardial infarction.However,the management of non-IRA remains controversial, and there is no consensus on the management of non-IRA in STEMI. FFR could be a new criteria in non-IRA PCI,since it could evaluate coronary flow reserve more intuitively than coronary angiography alone.

Long-segment lichen sclerosus (LS) urethral stricture is a challenge for urologists. Limited data are available for surgeons to make a surgical decision between Kulkarni and Asopa urethroplasty. In this retrospective study, we investigated the outcomes of these two procedures in patients with LS urethral stricture. Between January 2015 and December 2020, 77 patients with LS urethral stricture underwent Kulkarni and Asopa procedures for urethroplasty in the Department of Urology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine (Shanghai, China). Of the 77 patients, 42 (54.5%) underwent the Asopa procedure and 35 (45.5%) underwent the Kulkarni procedure. The overall complication rate was 34.2% in the Kulkarni group and 19.0% in the Asopa group, and no difference was observed ( P = 0.105). Among the complications, no statistical difference was observed in the incidence of urethral stricture recurrence ( P = 0.724) or glans dehiscence ( P = 0.246) except for postoperative meatus stenosis ( P = 0.020). However, the recurrence-free survival rate between the two procedures was significantly different ( P = 0.016). Cox survival analysis showed that antiplatelet/anticoagulant therapy use ( P = 0.020), diabetes ( P = 0.003), current/former smoking ( P = 0.019), coronary heart disease ( P < 0.001), and stricture length ( P = 0.028) may lead to a higher hazard ratio of complications. Even so, these two techniques can still provide acceptable results with their own advantages in the surgical treatment of LS urethral strictures. The surgical alternative should be considered comprehensively according to the patient characteristics and surgeon preferences. Moreover, our results showed that antiplatelet/anticoagulant therapy use, diabetes, coronary heart disease, current/former smoking, and stricture length may be contributing factors of complications. Therefore, patients with LS are advised to undergo early interventions for better therapeutic effects.
Male ; Humans ; Urethral Stricture/etiology* ; Retrospective Studies ; Constriction, Pathologic/surgery* ; Lichen Sclerosus et Atrophicus/surgery* ; Treatment Outcome ; Urologic Surgical Procedures, Male/methods* ; China ; Urethra/surgery* ; Postoperative Complications/etiology* ; Mouth Mucosa ; Diabetes Mellitus/etiology* ; Anticoagulants ; Coronary Disease

Migration of adventitial fibroblasts (AF) is involved in the neointimal formation which is one of the common pathological processes in several vascular diseases. The observation of whether the migratory response of AF from hypertensive animal is different from that of controls may provide an explanation of vascular remodeling in hypertension. We examined whether there is any difference between the migratory activity of AF derived from spontaneously hypertensive rat (SHR) and that from their normotensive counterpart Wistar-Kyoto rats (WKY). In addition, the role of osteopontin (OPN) in cell migration was also examined. Primary cultures of aortic adventitial fibroblasts were derived from SHR and age-matched WKY. Migration of fibroblasts was determined with the Transwell method. The mRNA expression level of OPN was measured by a real-time quantitative PCR. When compared with WKY-derived cells, migration of adventitial fibroblasts from SHR exhibited an increased response when stimulated by 10% serum (cell number per field 35.20+/-5.26 vs 22.2+/-3.27, p<0.05). Chemotaxis induced by 10 ng/ml bFGF showed a similar difference (cell number per field 30.23+/-4.54 vs 19.20+/-4.47, p<0.05). We also found that SHR-derived fibroblasts expressed a higher level of OPN mRNA than the cells from WKY (1863.23+/-43.91 vs 326.24+/-68.29, p<0.01). To verify if OPN is associated with the enhanced migratory ability in AF from SHR, we designed the antisense oligonucleotide of OPN. The results showed that the antisense OPN oligonucleotide significantly inhibited AF migration (cell number per field 38.60+/-5.98 vs 26.61+/-3.84, p<0.05), while sense and mismatch OPN oligonucleotide had no effect on cell migration. Therefore, the migration of adventitial fibroblasts appeared to be enhanced in cultures derived from SHR. OPN might be involved in the difference observed.
Animals ; Aorta ; cytology ; Cell Movement ; drug effects ; Cells, Cultured ; Fibroblasts ; cytology ; Hypertension ; physiopathology ; Male ; Osteopontin ; Phosphoproteins ; pharmacology ; Rats ; Rats, Inbred SHR ; Rats, Inbred WKY ; Sialoglycoproteins ; pharmacology

Abstract: Our previous work revealed berberine can significantly enhance the susceptibility of fluconazole against fluconazole-resistant Candida albicans, which suggested that berberine has synergistic antifungal activity with fluconazole. Preliminary SAR of berberine needs to be studied for the possibility of investigating its target and SAR, improving its drug-likeness, and exploring new scaffold. In this work, 13-substitutited benzyl berberine derivatives and N-benzyl isoquinoline analogues were synthesized and characterized by 1H NMR and MS. Their synergetic activity with fluconazole against fluconazole-resistant Candida albicans was evaluated in vitro. The 13-substitutited benzyl berberine derivatives 1a-1e exhibited comparable activity to berberine, which suggested that the introduction of functional groups to C-13 can maintain its activity. The N-benzyl isoquinolines, which were designed as analogues of berberine with its D ring opened, exhibited lower activity than berberine. However, compound 2b, 2c, and 4b showed moderate activity, which indicated that berberine may be deconstructed to new scaffold with synergistic antifungal activity with fluconazole. The results of our research may be helpful to the SAR studies on its other biological activities.
Antifungal Agents ; pharmacology ; Berberine ; pharmacology ; Candida albicans ; drug effects ; Drug Resistance, Fungal ; Drug Synergism ; Fluconazole ; pharmacology ; Isoquinolines ; pharmacology ; Microbial Sensitivity Tests

OBJECTIVETo evaluate the exploratory development of bone graft by titanium mesh with bone allograft in treatment of cervical spinal tuberculosis.

METHODSThirty two cases of cervical spinal tuberculosis treated with anterior radical debridement, decompression and inter fixation from January 2002 to January 2007 were included (at least two years follow-up). 18 male and 14 female, age from 18 to 72, mean 41.3 years old. 0.5 - 15.0 months before visit, mean 6.9 months. There were 13 cases in initial treatment group and 19 cases in retreatment group. All cases were divided into two groups (group A and group B) by resource of bone graft. Group A, titanium mesh with bone allograft, 17 cases. Group B, autograft with ilium, 15 cases. Operation time, blood loss, curing conditions, cervical curvature (absolute rotation angle, ARA), function of spinal cord and the rate for bone graft fusions in two groups were compared. The mean follow-up was 3.5 years (range 2 - 5 years).

RESULTSThe primary healing rate of incisions was 93.8% (30/32), and total healing rate was 96.9% (31/32). There were no significant differences in operation time or in blood loss between two groups (P > 0.05). Operation time and blood loss, 72 min/121 ml in group A and 90 min/198 ml in group B, there were significant differences between two groups (P < 0.05). In each group, there were significant differences in the function of spinal cord between preoperative and immediately post operative, between preoperative and follow-up, and between immediately post operative and follow-up (P < 0.05), and there were significant differences in ARA between preoperative and immediately post operative, and between preoperative and follow-up (P < 0.05), and there were no significant differences between immediately post operative and follow-up (P > 0.05). On preoperative, immediately post operative and follow-up, there were no significant differences in the function of spinal cord or in ARA between two groups (P > 0.05).

CONCLUSIONFor cervical spinal tuberculosis followed by effective individual chemotherapy, a good effect can be obtained by treated with radical debridement and bone allograft with titanium mesh.

Adolescent ; Adult ; Aged ; Bone Transplantation ; methods ; Cervical Vertebrae ; Female ; Follow-Up Studies ; Humans ; Male ; Middle Aged ; Retrospective Studies ; Surgical Mesh ; Titanium ; Transplantation, Homologous ; Treatment Outcome ; Tuberculosis, Spinal ; surgery ; Young Adult

OBJECTIVETo study the influence of thermochemotherapy on the activity of cytotoxic T lymphocyte (CTL) in peripheral blood of patients with oral maxillofacial cancer.

METHODSTwenty-one subjects with oral maxillofacial cancer were treated by thermochemotherapy, and the activity of CTL in peripheral blood was analyzed.

RESULTSThermochemotherapy can obviously enhance the activity of CTL (P<0.01).

CONCLUSIONThermochemotherapy can enhance the activity of CTL, thus enhance the patient's immune function. Therefore, it can enhance the antitumor response in whole body.

Humans ; Hyperthermia, Induced ; Mouth Neoplasms ; drug therapy ; T-Lymphocytes, Cytotoxic