Adult ; Brain ; anatomy & histology ; diagnostic imaging ; Female ; Humans ; Tomography, Emission-Computed, Single-Photon ; Tomography, X-Ray Computed

Objective To explore the feasibility and methodology of radiofrequency catheter ablation (RFCA) guided by 3D navigation system (Ensite-NavX) for right atrioventricular accessory pathway.Method Thirty-three cases of right accessory pathway atrioventricular reentrant tachycardia including 16 cases in right free wall,3 in right middle septum,14 in right posterior septum; 23 cases of dominant accessory pathway and 10 cases of concealed were treated by RFCA guided by NavX navigation.NavX navigation modeling method or spatial localization method was exploited to locate target positioning.Result All patients were successfully ablated without serious complications.Among them,25 cases were operated without exposure to X-ray,7 patients were exposed for several seconds to verify catheter position,1 case in right free wall was ablated under X-ray combined with Swartz sheath ablation.Conclusion Nonfluoroscopy or less fluoroscopy RFCA for right atrioventricular accessory pathway with Ensite-NavX is safe and feasible,modeling or spatial orientation method are helpful to locate the ablation target positioning.

This study was aimed to establish an optimized method to observe the synchronous changes of vascular tension and intracellular Casignal in the third-order branches of mesenteric arteries (sMA, diameter: 100-300 μm). The vascular tension and intracellular Casignal changes in response to potassium chloride (KCl), endothelin-1 (ET-1) and Gdwere detected using confocal wire myograph system and confocal laser scanning microscopy imaging technique, respectively. The experimental results were analyzed to explore the optimal experimental conditions. The results showed that KCl caused contraction in sMA significantly, and the intracellular Calevel of vascular smooth muscle cells (VSMCs) was also increased under 20× and 40× objective lens. Compared with those under the 40× objective lens, the Casignal change was larger and the fluorescence value was more stable under the 20× objective lens, whereas the Casignal change was not obvious under the 10× objective lens. ET-1 (1-10 nmol/L) caused concentration dependent contraction in sMA significantly, and the intracellular Casignal of VSMCs was also enhanced in a concentration dependent manner. Additionally, Gdsignificantly reduced the contraction of sMA and the intracellular Casignal of VSMCs caused by ET-1. The results suggest that the intracellular Casignal of VSMCs changes with vascular contraction or relaxation caused by the agonists or antagonists of Cachannels. We successfully recorded both changes synchronously using confocal wire myograph system and confocal laser scanning microscopy imaging technique at the same time. Based on the analysis of the experimental results, we concluded that 20× objective lens provides the best experimental condition. Compared to combination of vascular tone detection method and real-time cellular fluorescence imaging technique, the present synchronous method is convenient and helpful to reduce experimental error.