Gastrectomy ; methods ; Humans ; Pylorus ; surgery ; Stomach Neoplasms ; surgery

Objective Beta-amyloid (AB) plaque is one of the most important hallmarks of Alzhe-imer disease (AD). [N-methyl-11C]-[4'-methylaminophenyl]-6-hydroxybenzothiazole (11C-PIB) can have a strong binding potential (BP) of AB plaques in the brains of patients with dementia of Alzheimer type (DAT). This study was to investigate the value of rostral anterior cingnlate cortex (rACC) in diagnosing human AD with 11C-PIB PET imaging. Methods All the subjects were enrolled through ads by Washington University School of Medicine (WUSM) in USA. Clinical dementia rating (CDR) was the practical grading standard for AD. There were 129 cases of normal control (HC) with CDR =0 and 40 cases of AD patients with CDR >0 (but there were only 120 HC cases and 34 DAT patients with data of rACC PIB BP. All 169 cases had PIB BP data of other studied cerebral subregions). Brain subregions were localized with MRI. PET imaging was performed immediately after intravenous injection of11C-PIB. MRI and PET images were aligned and fused with the help of software. BP was calculated using the Logan graphical analysis and the cerebellar cortex as the reference tissue. The distribution characters of 11C-PIB BP in rACC were analyzed and compared with other brain subregions studied by other scholars with the same research cases. The corre-lation analysis, t-test or variance analysis were calculated with SPSS 11.5. Results rACC PIB BP of 120 HC and 34 AD patients was calculated, rACC PIB BP was statistically linear and positively correlated with CDR significantly (BP=0.2865±0.442,CDR=0.143±0.290,r=0.545,P<0.01). There was no sta-tistical difference among the rACC PIB BP of DAT patients (0.6719±0.1545 vs 0.8933±0.0880,inde-pendent-samples t-test in both groups of CDR =0.5and1, indicated t=-1.245,P>0.05), but signifi-cant difference was found between rACC PIB BPs of HC and DAT patients (0.1589±0.0219 vs 0.7370±0.1125, independent-samples t-test in both group of CDR=0 and>0,indicatedt=-7.998, P<0.01). The threshold of rACC PIB BP for differentiating DAT was 0.4592, greater than what could be thought as suffering from DAT with sensitivity 67.65 % (23/34) and specificity 88.33% (106/120). The BP values of both groups partially overlap each other. In all the studied cerebral subregions, the highest 3 subregions in mean PIB BP of DAT patients or in the difference of mean PIB BP between DAT patients and HC group were precuneus, rACC and prefrontal cortices. Conclusion rACC PIB BP can be used to differentiate DAT patients from normal persons; rACC, precuneus and prefrontal cortices are together the most sensitive brain subregions in the diagnosing DAT.

Objective To explore the clinical value of double collagenase injection in treating patients with lumbar disc herniation. Methods Thirty one patients with lumbar disc herniation to whom DCI had been applied were analyzed with 15 to 31 months follow-up. Results The therapeutic effects were excellent in 27, good in 3, fail in 1. The total effective rate was 96.7%.Conclusions DCI is a good method in treating lumbar disc herniation with high effectiveness, extensive indications, low complications and profitable clinical application prospectively.

Objective:To investigate the anti-tumor effect of C215gene transfected tumor vaccin e and monoclonal antibody-superantigen fusion protein C215Fab-SEA.Methods:B16 melonoma cells were transfected with the gene encoding a human colon adenoca rcinoma cell-surface glycoprotein C215 to produce a tumor vaccine,melanom a models were established in C57BL/6 mice to observe the antitumor effect of the tumor vaccine and the fusion protein C215Fab-SEA.Results:Tumor growth in mice treated with the vaccine tog e ther with the fusion protein C215Fab-SEA was significantly inhibited comp ared with the control group(P＜0.01),while the tumor growth in group treate d wi th the vaccine only and group treated with the fusion protein only were not obvi ously inhibited compared with the controls.Conclusion:The fusion protein C215Fab-SEA could enh ance the effect of C215-transfected tumor vaccine against the growth of C 215-negative tumors.

Objective:To investigate the anti tumor effect of C 215 gene transfected tumor vaccine and monoclonal antibody superantigen fusion protein C 215 Fab SEA.Methods:B16 melonoma cells were transfected with the gene encoding a human colon adenocarcinoma cell surface glycoprotein C 215 to produce a tumor vaccine,melanoma models were established in C57BL/6 mice to observe the antitumor effect of the tumor vaccine and the fusion protein C 215 Fab SEA.Results:Tumor growth in mice treated with the vaccine together with the fusion protein C 215 Fab SEA was significantly inhibited compared with the control group(P

Humans ; Saliva ; physiology ; Salivary Gland Diseases

Abortifacient Agents, Nonsteroidal ; pharmacology ; Animals ; Drugs, Chinese Herbal ; pharmacology ; Female ; Pinellia ; chemistry ; Plant Proteins ; pharmacology ; Pregnancy

Humans ; Methods ; Parotid Neoplasms ; surgery

By studying the varieties of peony and analyzing the prescription of Huangqin decoction, the authors explored the differences between Paeoniae Radix Alba and Radix Paeoniae Rubra in varieties, origin, processing method and clinical efficacy and compare their efficacies to define Paeoniae Radix Alba or Paeoniae Radix Rubra in Huangqin decoction recorded in Treatise on Febrile Diseases. In the study, the authors clarified the development and change of the variety in various historical periods according to the earliest ancient herbal book recording the variety and the development sequence of main herbs, and made clear the relations between Paeoniae Radix Alba and Paeoniae Radix Rubra. The modern application of Paeoniae Radix Alba started in the Song Dynasty. Although it was processed in different ways from Paeoniae radix Rubra, they shared the same original plant varieties. On the basis of the historical origin, botanical origin, producing place and processing method, the authors made clear the evolvement of peony varieties, discussed and analyzed the developments and changes for the combined to the separate administration of Paeoniae Radix Alba and Paeoniae Radix Rubra and defined the peony variety in Huangqin decoction. Through the textual research on ancient herbal books, the authors confirmed that more than 2 000 years ago, Paeoniae Radix Alba didn't appear when Treatise on Febrile Diseases was written. According to the records in Miscellaneous Records of Famous Physicians that "its roots were collected and dried in February and August", it was inferred that the use of Paeoniae Radix Rubra conformed to the historical facts.
China ; Drugs, Chinese Herbal ; chemistry ; history ; History, Ancient ; Medicine in Literature ; Plants, Medicinal ; chemistry

In the field of gene delivery, nonviral vectors are getting more attention due to the advantages which do not belong to viral vectors. But kinds of polypeptides are important to construct ideal nonviral vectors. The paper reviews the development in the research of polypeptides as components of nonviral vectors like as the backbone, the targeting moiety, the endosome membrane destabilizing agents and the nuclear localizing signals.