1.Stimulation of Chondrogenic Differentiation of Mesenchymal Stem Cells.
Da Ae YU ; Jin HAN ; Byung Soo KIM
International Journal of Stem Cells 2012;5(1):16-22
The methods for cartilage repair have been studied so far, yet many of them seem to have limitations due to the low regenerative capacity of articular cartilage. Mesenchymal stem cell (MSC) has been suggested as an alternative solution to remedy this challenging problem. MSCs, which have extensive differentiation capacity, can be induced to differentiate into chondrocytes under specific conditions. Particularly, this review focused on the effects of growth factors, cell-to-cell interactions and biomaterials in chondrogenesis of MSCs. Appropriate stimulations through these factors are crucial in differentiation and proliferation of MSCs. However, use of MSCs for cartilage repair has some drawbacks and risks, such as expression of hypertrophy-related genes in MSCs-derived chondrocytes and consequent calcification or cell death. Nevertheless, the clinical application of MSCs is expected in the future with advanced technology.
Biocompatible Materials
;
Cartilage
;
Cartilage, Articular
;
Cell Communication
;
Cell Death
;
Chondrocytes
;
Chondrogenesis
;
Intercellular Signaling Peptides and Proteins
;
Mesenchymal Stromal Cells
2.Lichen Striatus: Clinical Features and Treatment Outcomes of Topical Agent Use in 60 Pediatric Patients
Da-Ae YU ; Jungyoon OHN ; Ohsang KWON ; Kyu Han KIM
Korean Journal of Dermatology 2020;58(5):318-323
Background:
Lichen striatus (LS) is a linear, inflammatory dermatosis that mainly affects children. To date, there are limited data comparing treatment outcomes between topical agents for pediatric LS.
Objective:
To investigate the clinical features and treatment outcomes associated with the use of topical agents for pediatric LS.
Methods:
We retrospectively reviewed the medical records and clinical photographs of 60 pediatric patients classified into three groups according to the topical agent used. Treatment response was evaluated by comparing subjective symptoms and clinical photographs obtained at each visit.
Results:
A good response (>75% clinical improvement from baseline) was achieved in 55%, 46.2%, and 42.9% of patients from the calcipotriol, corticosteroid, and calcineurin inhibitor groups, respectively. There were no statistically significant differences in treatment duration among groups. However, when compared to other drugs, calcipotriol showed a trend towards shortening the disease course duration.
Conclusion
Topical use of calcipotriol, as well as corticosteroids and calcineurin inhibitors, may represent useful treatment options for pediatric LS, although further studies are required to confirm their efficacy and long-term safety.
3.Hypomelanosis of Ito with Multiple Congenital Anomalies
Da Ae YU ; Ohsang KWON ; Kyu Han KIM
Annals of Dermatology 2019;31(5):576-580
Hypomelanosis of Ito (HI) is a neurocutaneous disorder, also known as incontinentia pigmenti achromians. HI has been associated with chromosomal abnormalities, especially mosaicism. Herein, we report a case of HI with multiple congenital anomalies. A 2-month-old girl presented with multiple linear and whorling hypopigmentation on the face, trunk, and both extremities and patch alopecia on the scalp. Moreover, she had conical teeth, aniridia of the both eyes, and multiple musculoskeletal problems, including syndactyly and coccyx deviation. Cytogenetic analysis on peripheral blood was normal 46, XX, and no mutation was found in IKBKG gene test.
Alopecia
;
Aniridia
;
Chromosome Aberrations
;
Coccyx
;
Cytogenetic Analysis
;
Extremities
;
Female
;
Humans
;
Hypopigmentation
;
Infant
;
Karyotype
;
Mosaicism
;
Neurocutaneous Syndromes
;
Pigmentation Disorders
;
Scalp
;
Syndactyly
;
Tooth
4.A Case of Localized Trichorrhexis Nodosa Caused by Rubbing.
Da Ae YU ; Ji Young CHOI ; Min Woo KIM ; Ohsang KWON ; Hyun Sun YOON ; Soyun CHO ; Hyun Sun PARK
Korean Journal of Dermatology 2018;56(4):289-290
No abstract available.
Dermoscopy
;
Hair Diseases
;
Microscopy, Electron, Scanning
;
Pruritus
5.A Study on Vitamin C Content of Nutrition Emphasized Products.
Da Un JEONG ; Heon Ok LEE ; Young Kyoung KIM ; Ae Son OM
Korean Journal of Community Nutrition 2016;21(6):574-579
OBJECTIVES: Vitamin C has various functions such as antioxidative effect and supporting absorption of iron (Fe). Aim of this present study was to provide vitamin C nutrition information and to briefly evaluate absorption interaction of vitamin C and Fe content of vitamin C emphasized products. METHODS: Vitamin C emphasized foods including beverages, cereal, snacks, chocolate products, other cocoa products, and sugary products were examined by HPLC. Fe contents in samples after dry-ashing were examined by ICP. RESULTS: Vitamin C content ranges in various products tested were the following: beverages (n=11) 20.15 ± 0.08~845.41 ± 6.07 mg, cereal (n=11) 52.50 ± 0.23~262.50 ± 0.07 mg, snacks (n=1) 50.00 ± 0.25 mg, chocolate products (n=1) 311.73 ± 2.44 mg, other cocoa products (n=1) 311.73 ± 2.44 mg, other sugary products (n=2) 52.50 ± 0.23~262.50 ± 0.07 mg. Vitamin C (n=27) analysis values ranged from 82 to 450% of the labeled value. Vitamin C content in vitamin C emphasized food (n=6) was estimated 7.7 times~56.6 times more than Fe content. CONCLUSIONS: Analyzed samples ranged more than 80% of the labeled value in vitamin C emphasized products, which complied with food labeling regulation. But, beverages (n=3), cereal (n=4), chocolate products (n=1) were 2 times more than the labeled value. To provide accurate nutrition information, food manufactures should supervise nutrition labeling and understand the interactions between nutrients. Also, consumer should decide about the adequate amount of nutrient intake by thoroughly checking nutrition labeling.
Absorption
;
Ascorbic Acid*
;
Beverages
;
Cacao
;
Chromatography, High Pressure Liquid
;
Edible Grain
;
Food Labeling
;
Iron
;
Snacks
;
Vitamins*
6.Good Clinical Responders to Topical Timolol in Patients with Infantile Hemangiomas: A 7-Year Retrospective Study of 328 Korean Patients
Da-Ae YU ; Se Hee MIN ; Jaeryong SONG ; Jong Seo PARK ; Hanjae LEE ; Jungyoon OHN ; Kyu Han KIM
Annals of Dermatology 2022;34(5):360-369
Background:
Topical timolol is widely used for treatment of superficial infantile hemangioma (IH). However, little is known about factors that affect the response to topical timolol treatment.
Objective:
This study aimed to investigate the efficacy, safety, and predictive value for good response to topical timolol for IH.
Methods:
A retrospective review of medical records and clinical photos of 328 patients with IH treated with topical timolol 0.5% solution was conducted. Serial clinical photographs were compared with those at the initial visit using a 100-mm visual analogue scale (VAS). Treatment response was defined as an improvement of at least 75% from baseline in IH lesions within 12 months of treatment.
Results:
Overall, IH patients treated with topical timolol showed significant improvement from baseline, showing that the final VAS score within 12 months of treatment was 69.7±20.4. The multivariable logistic regression analysis showed age at initiation of treatment (p=0.007), length of gestation and fetal growth (p=0.03), depth (p=0.01), and flexural area (p=0.007) were significantly associated with treatment response. Only four patients (1.1%) reported local irritation.
Conclusion
This study demonstrated that topical timolol treatment was an effective and well-tolerated treatment for IHs. Physicians are encouraged to consider several patient- or lesional factors that might affect treatment response to achieve better clinical outcomes.
7.Analysis of Microbial Contamination and Antibacterial Effect Associated with Toothbrushes.
Ji Hyang KIM ; Da Ae KIM ; Hee Soo KIM ; Ji Yeon BAIK ; So Hee JU ; Seol Hee KIM
Journal of Dental Hygiene Science 2018;18(5):296-304
The purpose of this study was to propose a method for the effective management of toothbrush contamination. Toothbrush microbial contamination was analyzed according to the duration of toothbrush use, frequency of toothbrush use per day, and toothbrush storage location. We also analyzed the microbial reduction effect of vinegar, antimicrobial mouth rinse, bamboo salt, and baking soda, which are sterilization materials that can be easily used every day. We collected 45 toothbrushes from university dormitories from May to June 2018. To determine the degree of microbiological contamination with general bacteria, coliform bacteria, and Staphylococcus aureus, bristle samples were cultured at 36oC for 24 hours using 3M™ Petrifilm plates and then measured based on Petrifilm evaluation criteria. Toothbrush microorganisms were analyzed according to the duration of use, frequency of use per day, storage location, and effect of each sterilization material. General bacteria, coliforms, and S. aureus contamination increased with frequency and duration of use (p <0.05). In particular, S. aureus showed a statistically significant increase to 36.15 CFU/ml after 1 month, 504.23 CFU/ml after 2 months, and 2,386.67 CFU/ml after 3 months (p <0.05). We found that 1% vinegar was the most effective substance for reducing general bacteria, coliforms, and S. aureus. In addition, 1% antimicrobial mouth rinse solution applied for 5 minutes was the most effective in reducing S. aureus. It is crucial to recognize the importance of toothbrush care and store toothbrushes in a dry place and replace them periodically. We recommend use of vinegar and antimicrobial mouth rinse solution to disinfect toothbrushes. These should be applied as a 1% solution for at least 1 minute. Proper care of toothbrushes is important in maintaining oral health as well as overall health. Instructions on toothbrush care should be given when teaching children or adults how to brush teeth.
Acetic Acid
;
Adult
;
Bacteria
;
Child
;
Humans
;
Methods
;
Mouth
;
Oral Health
;
Oral Hygiene
;
Sodium Bicarbonate
;
Staphylococcus aureus
;
Sterilization
;
Tooth
;
Toothbrushing
8.Association between Thyroid Cancer and Advanced Colorectal Neoplasia.
Da Min KIM ; Jung Ha KIM ; Jun Young PARK ; Byung Woo JUN ; Lim Hwa SONG ; Hyun Ae JUNG ; Young Ho KIM
Intestinal Research 2011;9(3):206-210
BACKGROUND/AIMS: Colorectal cancer and thyroid cancer are common diseases with relatively higher survival rates compared with other cancers. The number of patients identified with colorectal cancer or thyroid cancer who develop multiple primary malignancy during long-term follow-up is increasing with advances in diagnostic techniques and treatment modalities. However, the association between colorectal cancer and thyroid cancer is uncertain, and few data have been reported in Korea. This study examined the association between thyroid cancer and colorectal neoplasm. METHODS: We retrospectively investigated 363 patients who underwent a colonoscopy, among patients diagnosed with thyroid cancer between January 2004 and December 2008 at Samsung Medical Center. The control group was comprised of 2,494 patients who underwent screening colonoscopy for the first time within the study period at the center for health promotion at Samsung Medical Center between March 2004 and December 2005. RESULTS: The detection rates in patients with thyroid cancer were 4.7% (17/363) for advanced adenomas and 2.8% (10/363) for colorectal cancer. In the control group, it was 3.2% (79/2,494) for advanced adenomas and 0.3% (7/2,494) for colorectal cancer. A multivariate analysis revealed that the presence of thyroid cancer had an odds ratio of 1.893 (95% confidence interval, 0.868-4.128, P=0.109) in favor of finding at least one advanced colorectal neoplasm. CONCLUSIONS: The results indicate that thyroid cancer is not associated with advanced colorectal neoplasm. Survivors of thyroid and colorectal cancer live longer and hence are at risk for second primary cancers. Therefore, further studies that prospectively evaluate the association between thyroid cancer and colorectal advanced neoplasm are needed.
Adenoma
;
Colonoscopy
;
Colorectal Neoplasms
;
Health Promotion
;
Humans
;
Korea
;
Mass Screening
;
Multivariate Analysis
;
Neoplasms, Second Primary
;
Odds Ratio
;
Retrospective Studies
;
Survival Rate
;
Survivors
;
Thyroid Gland
;
Thyroid Neoplasms
9.Effect of red vinegar drink on the surface of sound enamel.
Da Eun KIM ; Kyung Hee KIM ; Ae Ok KIM ; Seong Soog JEONG ; Choong Ho CHOI ; Suk Jin HONG ; Hyo Suck HONG
Journal of Korean Academy of Oral Health 2014;38(3):184-190
OBJECTIVES: The aim of this study was to evaluate the effect of red vinegar drink on sound enamel surface. METHODS: Commercially available red vinegar drink was used for the study. Firstly, pH values were measured in commercially available red vinegar drinks. Secondly, four groups; mineral water as the control group and red vinegar drink, red vinegar drink + mineral water (mixing ratio, 1:3), red vinegar drink + milk (mixing ratio,1:4) as the experimental group were selected. Forty specimens of bovine teeth were made and then divided into the four groups and treated with the test drinks for 1, 15, 30 and 60 minutes. The surface microhardness (vickers hardness number, VHN) was measured using the microhardness tester before and after the treatments. The surface of specimens was observed with Scanning Electron Microscopy (SEM). RESULTS: The average pH of red vinegar drinks was 2.91+/-0.02. The change values (before treatment - after treatment of surface microhardness of enamel surface) were significantly difference among groups (P<0.05). There was no significant difference between control and red vinegar drink + milk (1:4) and there was the significant difference between control group and red vinegar drink groups, and control and red vinegar drink + mineral water (1:3) groups. In SEM, damage of enamel surface was observed in Red vinegar and Red vinegar+Mineral water group. CONCLUSIONS: The results showed that the all experimental red vinegar drinks, except red vinegar drink + milk (1:4) can reduce the surface microhardness of sound enamel. Thus, it is suggested that the red vinegar drink with milk could be recommended the preventive eating method for reducing the risk of dental erosion on the red vinegar drink diet.
Acetic Acid*
;
Dental Enamel*
;
Diet
;
Eating
;
Hardness
;
Hydrogen-Ion Concentration
;
Microscopy, Electron, Scanning
;
Milk
;
Mineral Waters
;
Tooth
;
Water
10.The erosive effect of commercial red ginseng beverages on bovine enamel surfaces.
Da Eun KIM ; Kyung Hee KIM ; Ae Ok KIM ; Seong Soog JEONG ; Choong Ho CHOI ; Suk Jin HONG
Journal of Korean Academy of Oral Health 2016;40(3):198-205
OBJECTIVES: This study was conducted to investigate the influence of several commercial red ginseng beverages on the surface of healthy teeth and to confirm the anti-erosive effect of added calcium. METHODS: For the experimental group selection, the pH of red ginseng beverages on the market were measured and the mean pH was calculated. Beverages with the lowest pH (Dong Wha Hongsam Gold; red ginseng beverage group with pH 2.98), mid-level pH (Kwangdong Jin Hongsam Gold; red ginseng beverage group with pH 3.61), and the highest pH (Hongsam Han Ppuri; red ginseng beverage group with pH 5.34) were selected as the experimental groups. In order to confirm the anti-erosive effect of added calcium, we added 1% calcium to the product with the lowest pH (red ginseng beverage group with pH 2.98+1% Ca) and included the product in the experimental group. Jeju Samdasoo and Coca Cola were used as the negative and positive control groups, respectively. We soaked healthy bovine teeth samples in the selected six beverages for 1, 3, 5, 10, 15, and 30 minutes. The surface microhardness (VHN, Vickers hardness number) and the surface roughness (center line average roughness, Ra) of each sample were measured, and the surface features were observed with a scanning electron microscope (SEM). RESULTS: The change in the surface micro-hardness (△VHN) of teeth after 30 minutes of soaking in each beverage was the highest in the positive control group (60.99±8.99), followed by A (41.63±8.96), B (30.64±8.21), and the negative control group (―4.48±7.29) (P<0.05). No significant difference was observed in group C (―18.79±10.11) or D (―16.40±7.89). Surface roughness (Ra) exhibited significant differences between each group (P<0.05). Surface roughness (Ra) was high in A (102.88±26.34) and B (67.76±39.89), as well as in the positive control group (101.21±39.59). In contrast, C (30.80±28.49) and D (25.05±10.79) showed low surface roughness values similar to the negative control group (23.77±22.48). Following SEM examination, severe cracks were observed between the crystals in groups A and B; such characteristics were similar to those of the positive control group. CONCLUSIONS: Red ginseng beverages with low pH were shown to erode the surface of the teeth. When calcium was added to the red ginseng beverages, a decrease in tooth erosion was observed. Therefore, the possibility of tooth erosion should be considered when drinking red ginseng beverages. Furthermore, the addition of calcium to red ginseng beverages can be an alternative solution to suppress tooth erosion.
Beverages*
;
Calcium
;
Coca
;
Cola
;
Cytochrome P-450 CYP1A1
;
Dental Enamel*
;
Drinking
;
Hardness
;
Hydrogen-Ion Concentration
;
Panax*
;
Tooth
;
Tooth Erosion