1.Detection of male-specific DNA by polymerase chain reaction.
Korean Journal of Perinatology 1993;4(3):391-400
No abstract available.
DNA*
;
Polymerase Chain Reaction*
2.Optimixation of per for amplification of D1S80 (PMCT118) allelles by using vent DNA polymerase.
Journal of Medical Research 2001;16(3):20-23
D1S80 locus is highly polymorphic and has been used worldwide as an important marker for forensic, medical analysis and paternity tests. Vent DNA Polymerase is a high-fidelity thermophilic DNA polymerase. The fidelity of Vent DNA polymerase is 5 to 15 folds higher than that observed for Tag DNA polymerase. This high fidelity derives in part from an integral 3’-5’ proofreading exonuclease activity in Vent DNA polymerase. Greater than 90% of the polymerase activity remains after one hour incubation at 95oC. Because of these advantages, we want to apply Vent DNA polymerase for the amplification of D1S80 alleles by PCR. Our study showed that PCR condition for Vent DNA polymerase are rather different than for Tag DNA polymerase. The amplification of D1S80 alleles with Vent DNA polymerase is optimal with the following parameters: 1. A final concentration of MgSO4 in PCR mixture of 4 mM. 2. An annealing temperature for the specific D1S80 primers 68oC. 3. A final concentration of dNTPs of 200mcM.
DNA polymerase
;
Alleles
3.Detection of human immunodeficiency virus-1 and -2 DNA in seropositive Koreans by two-step polymerase chain reaction and hybridization with digoxigenin-probes.
Tai Gyu KIM ; Hoon HAN ; Gum Ryong KIM ; Chun KANG ; Yung Oh SHIN
Journal of the Korean Society of Virology 1992;22(1):81-90
No abstract available.
DNA*
;
Humans*
;
Polymerase Chain Reaction*
4.DNA Diagnosis Using Polymerase Chain Reaction.
Yeungnam University Journal of Medicine 1991;8(2):13-23
No abstract available.
Diagnosis*
;
DNA*
;
Polymerase Chain Reaction*
5.Detection of mutations of hemophilia A by PCR-RFLP with Vent DNA polymerase
Journal of Medical Research 2005;38(5):10-14
Study on blood samples of 29 patients with hemophilia A and 25 normal people to determine the mutation of intron 18 and 19 of factor VIII by PCR-RFLP using Vent DNA polymerase assay. PCR and RFLP methods were used. Results: 16/29 (55%) of haemophilia A patients has the mutation in intron 18 of factor VIII gene and none of them has the mutation in intron 19. This PCR-RFLP assay can be applied for detection of mutations and these methods can be used for carrier analysis and prenatal diagnosis of hemophilia A.
Hemophilia A
;
Polymerase Chain Reaction
;
DNA
6.Observation on the DNA polymorphism by polymerase chain reaction for carrier testing of hemophilia A.
Kyung Soon SONG ; Baek Soo KIM ; Samuel Y LEE
Korean Journal of Clinical Pathology 1991;11(2):381-386
No abstract available.
DNA*
;
Hemophilia A*
;
Polymerase Chain Reaction*
7.Properties of Hepatitis B Virus Associated DNA Polymerase.
Yonsei Medical Journal 1985;26(2):175-183
The nature of hepatitis B virus (HBV) particle associated DNA polymerase was studied in relation to various enzyme inhibitors including antiviral agents. HBV DNA polymerase required high concentration of MgCl2(> 30 mM) and neutral pH for its full activity. p-chloromercuribenzoate was a strong inhibitor (85% inhibition at 1 mM) but N-ethylmaleimide had much less inhibitory effect (20% inhibition at 10 mM). Phosphonoformic acid showed the greatest inhibitory effect on HBV-DNA polymerase (almost complete inhibition at 100 microM) among phosphocompounds tested. Adenine arabinoside triphosphate (ara-ATP) and cytosine arabinoside triphosphate (ara-CTP) were competitive inhibitors with respect to their respective deoxyribonucleoside triphosphate (dATP and dCTP). Ara-CPT was a stronger inhibitor of HBV-DNA polymerase compared to ara-ATP. Ki values for ara-ATP and ara-CTP were 15.0 microM and 11.7 microM , respectively. HBV-DNA polymerase is characteristic in its ionic requirements and susceptibilities to certain inhibitors.
DNA-Directed DNA Polymerase/antagonists & inhibitors
;
DNA-Directed DNA Polymerase/metabolism*
;
Hepatitis B Virus/enzymology*
;
Human
8.Detection of cytomegalovirus DNA by polymerase chain reaction in renal tissues from various glomerulonephritis.
Jae Hoon SONG ; Won Suk YANG ; Soon Bae KIM ; Bin YOO ; Yoo Kyum KIM ; Chang Ki HONG ; Jung Sik PARK
Korean Journal of Infectious Diseases 1993;25(2):151-157
No abstract available.
Cytomegalovirus*
;
DNA*
;
Glomerulonephritis*
;
Polymerase Chain Reaction*
9.Comparison of DNA preparation methods from mycobacterium tuberculosis in clinical specimens for polymerase chain reaction.
Kyeong Han YOON ; Tae Yoon LEE ; Sang Nae CHO ; Dook Soon KIM ; Dong Hyun CHUNG ; Joo Deuk KIM
Journal of the Korean Society for Microbiology 1991;26(2):159-166
No abstract available.
DNA*
;
Mycobacterium tuberculosis*
;
Mycobacterium*
;
Polymerase Chain Reaction*
10.Evaluation of Nine Non-CODIS MiniSTR Loci to aid Analysis of Degraded DNA.
Ukhee CHUNG ; Hwan Young LEE ; Myung Jin PARK ; Woo Ick YANG ; Sang Ho CHO ; Kyoung Jin SHIN
Korean Journal of Legal Medicine 2006;30(2):160-167
For highly degraded DNA samples of forensic casework, new miniSTR PCR systems have been developed to supplement the current CODIS STRs. In the present study, we established the three miniplexes for nine miniSTRs (NC01 : D10S1248, D14S1434 and D22S1045; NC02 : D1S1677, D2S441 and D4S2364; and NC03 : D3S3053, D6S474 and D20S482) which had been previously suggested by Butler group (NIST, Gaitherburg, MD, USA). To evaluate the usefulness of the nine miniSTRs in analysis of degraded DNA, the sensitivity and efficacy of the three miniplexes were determined and then compared with those of the BigMini STR system which consists of six CODIS miniSTRs (TH01, CSF1PO, FGA, TPOX, D7S820, and D21S11). The three miniplexes gave better results in both the sensitivity test and efficiency test in comparison with BigMini. In the sensitivity test using serially diluted standard DNA, most loci in the three miniplexes showed reliable results for samples containing 50 pg of DNA and some even showed good sensitivity for samples containing 30 pg of DNA. Additionally, the three miniplexes generated useful profiles for both enzymatically degraded DNA and 50-year old skeletal remain samples. Among the nine miniSTRs, D4S2364, D3S3053, D14S1434, and D1S1677 produced the most successful DNA profiles for old skeletal remains. These results suggest that new miniSTRs could be useful supplements to the 13 CODIS STRs for forensic analysis of degraded DNA.
DNA*
;
Humans
;
Middle Aged
;
Polymerase Chain Reaction