1.Application of DNA labeling technology in forensic botany.
Xian ZNANG ; Jing-Lin LI ; Xiang-Yu ZHANG
Journal of Forensic Medicine 2008;24(6):457-460
Forensic botany is a study of judicial plant evidence. Recently, researches on DNA labeling technology have been a mainstream of forensic botany. The article systematically reviews various types of DNA labeling techniques in forensic botany with enumerated practical cases, as well as the potential forensic application of each individual technique. The advantages of the DNA labeling technology over traditional morphological taxonomic methods are also summarized.
Amplified Fragment Length Polymorphism Analysis
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Botany/methods*
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DNA Fingerprinting/methods*
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DNA, Plant/analysis*
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Forensic Genetics
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Minisatellite Repeats
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Random Amplified Polymorphic DNA Technique
2.Establishment of fingerprint database for drug-resistant strain of Acinetobacter baumannii by AFLP method.
Nong HU ; Ting CAI ; Shun ZHANG
Journal of Zhejiang University. Medical sciences 2007;36(6):537-542
OBJECTIVETo establish fingerprint database of drug-resistant strain of Acinetobacter baumannii with AFLP genotype.
METHODSTwenty-seven strains of A. baumannii were clinically isolated. The microbial sensitivity to 13 antibiotics was analyzed according to NCCLS 2004. The genotype was analyzed by amplified fragment length polymorphism (AFLP) method.
RESULTSThe results were consistent with those with AFLP database.
CONCLUSIONThe database of AFLP fingerprint may have practical value for identification of drug-resistant Acinetobacter baumannii.
Acinetobacter baumannii ; genetics ; Amplified Fragment Length Polymorphism Analysis ; DNA Fingerprinting ; DNA, Bacterial ; genetics ; Databases, Nucleic Acid ; Drug Resistance, Bacterial ; genetics ; Genotype ; Microbial Sensitivity Tests ; Polymorphism, Genetic
3.An Analysis of the Victim's Identification Results from Commercial Aircraft Accidents in Guam.
Korean Journal of Aerospace and Environmental Medicine 1998;8(3):262-275
No abstract available.
Aircraft*
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DNA Fingerprinting
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Guam*
4.DNA typing of human papillomavirus in laryngeal papilloma.
Ju Young KIM ; Mun Su JANG ; Mun Sik YOO ; Jang Yuorl YOO ; Chan Il PARK
Korean Journal of Otolaryngology - Head and Neck Surgery 1993;36(3):355-360
No abstract available.
DNA Fingerprinting*
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DNA*
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Humans*
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Papilloma*
6.Comparative Study for DNA Extraction Methods from femurs in case of naming unidentified bodies.
Eung Soo KIM ; Dong Sub LEE ; Jin Hee KIM ; Moon Hee PARK ; Hee Jung AHN ; Young Joo KIM ; Dae Yeol KIM ; Sung Jin CHO ; Byung Ha CHOI ; Young Shik CHOI
Korean Journal of Legal Medicine 2007;31(1):41-44
In historical cases, missing persons' identification, mass disasters, and ancient DNA investigations, bone samples are often the only and always the best biological materials available for DNA typing. There are many protocols for the extraction of DNA from bone samples. In our experiment, we tested three DNA extraction protocols: Ethanol down method, Microcon(R) YM-100 and DNA IQ(TM) system. We have found that ethanol down method is not suitable to extract DNA from bone samples and Microcon(R) YM-100 is the best among these three mothods. DNA IQ(TM) System did not show better result but needed less time and labor than Microcon(R) YM-100. So, we expect using DNA IQ(TM) System is useful in case of bone samples which are not severely contaminated .
Disasters
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DNA Fingerprinting
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DNA*
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Ethanol
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Femur*
7.Korean Guidelines for DNA Typing in Forensic Field.
Seung Bum SEO ; Soong Deok LEE
Korean Journal of Legal Medicine 2009;33(1):89-90
The field of forensic DNA requires not only genetic typing but also social systems. The systems include the establishment of Korean guidelines for DNA typing. There were some publications relating DNA typing guidelines in 2001 and 2002, but the works could not be sustained. Although KOLAS and KIGTE are operating now, we can hardly say that these are optimal for forensic purposes. There also have been foreign publications about the DNA typing guidelines. Considering various differences among nationality, however, we need to establish suitable guidelines for Korean. Therefore, expert members in forensic society should be concern about Korean guidelines for DNA typing and participate in this work.
DNA
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DNA Fingerprinting
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Ethnic Groups
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Humans
8.DNA Profiling via Short Tandem Repeat Analysis by Using Serum Samples.
Ji Hyun LEE ; Hye Young LEE ; Sohee CHO ; Joo Youn CHO ; In Jin JANG ; Soong Deok LEE
Korean Journal of Legal Medicine 2013;37(4):220-223
Serum is free of cellular components. Because DNA is located in the nuclei or mitochondria of cells, serum could be assumed DNA free. Few previously published case reports to date have used serum for DNA typing. Here, we report on human genotyping via short tandem repeat (STR) analysis using serum as a sample, and discuss problems involved in the process.
DNA Fingerprinting*
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DNA*
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Humans
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Microsatellite Repeats*
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Mitochondria
10.Analysis and Discussion on Calculating Likelihood Ratio of DNA Mixture.
Ying LIU ; He REN ; Lin-lin GAO ; Yan SHI ; Chong CHEN ; Ya-cheng LIU
Journal of Forensic Medicine 2015;31(6):441-444
OBJECTIVE:
To analyze and discuss four methods of calculating likelihood ratio of DNA mixture.
METHODS:
In the case with CNAS-T0757 proficiency testing in 2013, the likelihood ratios were calculated and compared among four methods, including unrestricted combinatorial method, Clayton's method, p2 principle method, and recommendations from ISFG.
RESULTS:
The likelihood ratios were maximum by Clayton's method and recommendations from ISFO, followed by result of the unrestricted combinational method. The minimum likelihood ratio was obtained by p2 principle.
CONCLUSION
The unrestricted combinational method could give fUrthest consideration to both information preservation and appraiser protection.
DNA/genetics*
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DNA Fingerprinting
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Humans
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Likelihood Functions