1.Heteroplasmy Pattern Within D-loop Region of Mitochondrial DNA in Koreans.
Chong Min CHOUNG ; Byoung Su JANG ; Zhe LI ; Aihua ZHANG ; Yoon Seong LEE ; Jung Bin LEE ; Soong Deok LEE
Korean Journal of Legal Medicine 2005;29(2):189-195
Mitochondrial DNA (mtDNA) sequencing has been validated as a useful tool in forensics. However, there are several aspects that need to be considered in order for it to be used as firm evidence in a courtroom. Heteroplasmy is one of those. Heteroplasmy means the status that different mtDNA populations exist in a single individual, tissue, cell or even mitochondrion. As the underlying phenomenon of heteroplasmy and polymorphism is similar, care must be taken in analyzing the data from the samples with heteroplasmy. Heteroplasmy can reinforce the use of mtDNA however if we know a lot about it. For this much has to be revealed. In this we screened 494 unrelated Korean using DGGE for the D-loop to determine how frequent heteroplasmy is, and the heteroplasmy pattern was confirmed through sequencing. This paper discussed the results of a comparison with other reports along with several aspects of heteroplasmy.
DNA, Mitochondrial*
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Mitochondria
2.Three New Monotypic Genera of the Caloplacoid Lichens (Teloschistaceae, Lichen-Forming Ascomycetes).
Sergii Y KONDRATYUK ; Laszlo LOKOS ; Jung A KIM ; Anna S KONDRATIUK ; Min Hye JEONG ; Seol Hwa JANG ; Soon Ok OH ; Jae Seoun HUR
Mycobiology 2015;43(3):195-202
Three monophyletic branches are strongly supported in a phylogenetic analysis of the Teloschistaceae based on combined data sets of internal transcribed spacer and large subunit nrDNA and 12S small subunit mtDNA sequences. These are described as new monotypic genera: Jasonhuria S. Y. Kondr., L. Lokos et S. -O. Oh, Loekoesia S. Y. Kondr., S. -O. Oh et J. -S. Hur and Olegblumia S. Y. Kondr., L. Lokos et J. -S. Hur. Three new combinations for the type species of these genera are proposed.
Dataset
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DNA, Mitochondrial
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Lichens*
3.The Author Reply: Mitochondrial Ophthalmoplegia Is Not Only due to mtDNA Deletions
Yonsei Medical Journal 2019;60(2):232-233
No abstract available.
DNA, Mitochondrial
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Ophthalmoplegia
4.Mitochondrial Ophthalmoplegia Is Not Only due to mtDNA Deletions
Yonsei Medical Journal 2019;60(2):230-231
No abstract available.
DNA, Mitochondrial
;
Ophthalmoplegia
5.Analysis of Nuclear Mitochondrial DNA Segments of Nine Plant Species: Size, Distribution, and Insertion Loci.
Genomics & Informatics 2016;14(3):90-95
Nuclear mitochondrial DNA segment (Numt) insertion describes a well-known phenomenon of mitochondrial DNA transfer into a eukaryotic nuclear genome. However, it has not been well understood, especially in plants. Numt insertion patterns vary from species to species in different kingdoms. In this study, the patterns were surveyed in nine plant species, and we found some tip-offs. First, when the mitochondrial genome size is relatively large, the portion of the longer Numt is also larger than the short one. Second, the whole genome duplication event increases the ratio of the shorter Numt portion in the size distribution. Third, Numt insertions are enriched in exon regions. This analysis may be helpful for understanding plant evolution.
DNA, Mitochondrial*
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Exons
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Genome
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Genome, Mitochondrial
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Plants*
7.Analysis of Mitochondrial DNA in Patients with Essential Tremor.
Uhn LEE ; Young Mi YOO ; Chan Jong YOO
Journal of Korean Neurosurgical Society 2000;29(2):188-195
No abstract available.
DNA, Mitochondrial*
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Essential Tremor*
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Humans
8.Searching for Appropriate Statistical Parameters for Validation of Mitochondrial DNA Database.
Chong Min CHOUNG ; Ji Hyun LEE ; Sohee CHO ; Soong Deok LEE
Korean Journal of Legal Medicine 2014;38(2):59-65
Recently, studies on mitochondrial DNA (mtDNA) have increased rapidly. Conventional parameters, such as diversity index, pairwise comparison, are used to interpret and validate data on autosomal DNA; however, the use of these parameters to validate data from mitochondrial DNA databases (mtDNA DBs) needs to be verified because of the different transmission patterns of mtDNA. This study was done to verify the use of these conventional parameters and to test the "coverage concept" for a new parameter. The mtDNA DB is not very big; however, it is necessary to check how the change in parameters corresponds to the DB size. For this, we artificially rearranged a Korean DB into several small sub-DBs of variable sizes. The results show that the diversity in nucleotide variations and the different haplotype numbers do not vary as the size of DB increases. However, the "coverage" changed a lot. The coverage increased from 0.113 in a DB of 100 people to 0.260 in a DB of 653 people. Additionally, using the "coverage concept", we predicted how the total number of haplotypes changed with variations in the sub-DB size and compared the predicted result with final result. In conclusion, "coverage", in addition to conventional statistical parameters, can be used to check the usability of an mtDNA DB. Finally, we tried to predict the size of the whole mtDNA number in Korea using "saturation concept".
DNA
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DNA, Mitochondrial*
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Haplotypes
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Korea
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Phylogeny
9.Statistical Evaluation of Lineage Markers in Individual Identification.
Hyo Jung LEE ; Soong Deok LEE ; Seung Hwan LEE ; Su Jeong PARK ; Su Jin JEONG ; Jae Won LEE
Korean Journal of Legal Medicine 2014;38(2):39-47
Mitochondrial DNA (mt DNA) and the non-recombining region of the Y chromosome are passed down, unaltered, from generation to generation, matrilineally and patrilineally, respectively. Therefore, the Y-chromosome DNA and mtDNA are known as lineage markers, and they play important roles in studies based on human migration and evolutionary history. Y-chromosome DNA is used in forensic analysis to identify individuals involved in cases of sexual assault. In this paper, we review the methods of statistical evaluation of lineage markers used in forensic identification. We also review the combined approach of autosomal and lineage marker evidence.
DNA
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DNA, Mitochondrial
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Human Migration
;
Y Chromosome