1.The efficacy of psychological intervention on dental anxiety
Chinese Journal of Behavioral Medicine and Brain Science 2001;10(3):182-183
Objective To explore the effect of the psychological intervention on the treatment of dental anxiety (DA).Methods Measured with Corah's dental anxiety scale , 316DA patients were selected from department of dentistry and were divided into two groups: the intervention group and the control group . The psychological intervention as well as regular dental therapeutics were applied to the patients in the intervention group , whereas only regular dental therapeutics were used to those in the control group.Results The patients had a lower clinical dental anxiety in the intervention group , significantly different from that of the control group ( P< 0.01). After the treatment , more patients in the intervention group had a slower pulse than those in the control group and fewer had a faster pulse .The results also showed that the psychological intervention had a better curative effect on male patients than female ( P<0.05). Conclusion The psychological intervention was efficient to patients with dental anxiety.
2.Application of SM22α promoter and enhancer against angiostenosis following coronary artery bypass grafting
Chinese Journal of Tissue Engineering Research 2010;14(5):919-922
BACKGROUND:Great saphenous vein is the most common vascular material in coronary artery bypass grafting, however, the graft neointimal hyperplasia and subsequent atherosclerosis result in angiostegnosis, which affect the long-term efficacy. OBJECTIVE: To analyze the pathophysiology and mechanism of the angiostenosis, additionally, to construct smooth muscle cell specific SM22a promoter and SMHC enhancer. METHODS: The database of PubMed, HighWire and CNKI were retrieved with key words of "SM22α promoter, SMHC enhancer, stenosis, Pi3k and RNAi/RNA interference". The language was confined to English and Chinese. The literatures correlated with angiostenosis, VSMC-specific SM22α promoter and enhancer were selected. The reviews and overlapped researches were excluded. In vascular smooth muscle cell proliferation and apoptosis of graft neointimal hyperplasia, as well as the extent of graft thrombosis were considered as evaluation index. RESULTS AND CONCLUSION: More than 8 730 literatures were searched by the computer. According to inclusion and exclusion criteria, the SM22α promoter and angiostenosis were analyzed. The neointimal hyperplasia and thrombosis had a strong impact on prospective efficacy after coronary artery bypass grafting. The PI3K-Akt-mTOR pathway was a crucial signal passage which regulated cell proliferation and migration. RNA interference targeting Pik3cb can efficiently suppress the neointimal hyperplasia through down regulating the PI3K-Akt-mTOR pathway. The shRNA eukaryon expression plasmid vectors targeting rat Pik3cb were constructed using VSMC-specific SM22α promoter. On the one hand, it can suppress the neointimal hyperplasia, and on the other, it can prevent the occurrence of thrombosis.SM22a is a specific gene in vascular smooth muscle cell. The studies that applied VSMC-specific SM22α promoter/enhancer to vascular grafting provide a new strategy to prevent angiostenosis and thrombosis.
3.Repair finger with the second digit lateral shank flap through free transplantation of lateral defect
Chinese Journal of Postgraduates of Medicine 2015;38(10):730-733
Objective To introduce an ideal method of repairing of finger volar skin defect. Methods Thirty-five patients whose finger was repaired by second digit lateral shank flap through free transplantation of lateral defect were selected. Twenty-four cases were male and 11 cases were female, with age of 21 to 45 years old. Cutting injury was in 7 cases, crush injury was in 16 cases, degloving injury was in 9 cases, and firearm injury was in 3 cases. Skin defect area was 2.0 cm × 1.5 cm-4.0 cm ×2.5 cm. The course was 1 h to 5 d. Donor area was repaired by thick skin flap. Results The postoperative wound of 35 cases all healed in stage Ⅰ, and skin flap transplantation and the donor skin grafts survived. Twenty-nine cases received follow-up for 5-26 (14.5±5.0) months. The skin flap had good appearance and good texture, with two points discrimination of 5-8 (6.7±1.1) mm. According to the Chinese medical association upper part of the function evaluation trial standard: excellent was in 19 fingers, good was in 8 fingers, and the excellent and good rate was 93.1% (27/29). Walk, run, jump functions of donor foot were normal. Conclusions Repairing finger with the second digit lateral shank flap through free transplantation of lateral defect has small area damage, and damage to the area around the organization is small. The effect of repairing is good, and can achieve the aim of the palm side repairing.
4.Internet Addiction Disorder of Vocational College Freshmen and Its Relationship to Childhood Psychological Abuse and Neglect
Chinese Journal of Clinical Psychology 1993;0(01):-
Objective:To survey the prevalence of internet addiction disorder(IAD)of vocational college freshmen and analyze its relationship to their childhood abuse and neglect.Methods:IAT,SCL-90 and CPANS were used to investigate 740 freshmen of Yongzhou Technical Vocational College.Results:The rate of IAD among 740 freshmen is 3.2%.The factor scores of IAD students were significantly higher than those of normal students(P
5.Effects of geldanamycin on expression of HSP,mutant p53 and CDK4 in human breast cancer cells
Journal of Third Military Medical University 2003;0(13):-
Objective To investigate the effect of geldanamycin(GA) on the expression of HSP(HSP90,HSP70,HSP27) and oncoprotein(mutant p53,CDK4) in human breast cancer cell line MDA-MB-435s.Methods Proliferation of MDA-MB-435s cells was measured with MTT assay.The mRNA expression levels of HSP90,HSP70 and HSP27 were detected by RT-PCR.The protein expression levels of HSP90,HSP70,HSP27,mutant p53 and CDK4 were detected by Western blot.Results GA inhibited the proliferation of MDA-MB-435s cells in a time-dose dependent manner.The expression level of mRNA and protein of HSP90,HSP70 and HSP27 were increased,and the increasing of HSP70 was the most significant,while the protein expression level of mutant p53 and CDK4 in MDA-MB-435s cells were reduced obviously after 48-hour treatment with 400 nmol/L GA as compared with that of control cells.Conclusion GA inhibits the proliferation of MDA-MB-435s cells by down-regulating the level of mutant p53 and CDK4 protein,and GA involves in the protection of cell stress through increasing the expression of HSP90,HSP70 and HSP27.
6.Acalypha decoction in prevention and treatment of trinitrobenzene sulfonic acid induced ulcerative colitis in rats
Academic Journal of Second Military Medical University 1985;0(05):-
Objective:To study the preventive and therapeutic effects of Acalypha decoction on 2,4,6-trinitrobenzene sulfonic acid induced ulcerative colitis(UC) in rats.Methods: Totally 70 male SD rats were equally randomized into 7 groups, and UC was induced by intrarectal administration of TNBS (125 mg/kg in 50% ethanol). Six hours later the rats were given 3 different doses of Acapha decoction (2.5, 6.5, 9.5 g crude drug/kg body weight) daily for 10 d.Some rats were pretreated with Acalypha decoction (6.5 g crude drug/kg body weight) 3 d before the colitis was induced. Sulfasalazine was used as the standard drug for comparison. The efficacy of Acalypha decoction was assessed through the following parameters: bodyweight loss, diarrhea and bloody stool, macroscopic score,histological changes,and MPO activity in colon tissue. Results: Acalypha decoction treatment(9.5 g/kg, 6.5 g/kg) and prevention administration (6.5 g/kg) significantly inhibited the bodyweight loss and occurrence of diarrhea and rectal bleeding in the rats(P
7.Research progress in chemical induced animal models of inflammatory bowel disease
Chinese Pharmacological Bulletin 1986;0(05):-
In inflammatory bowel disease, the experimental model s have been proven to be important tools to investigate the potential therapeuti c agent and to study the mechanism of pathogenesis. Literatures available were r eviewed on the methods of induction, characteristic and availability of all kind s of chemically induced IBD animal models. It would provide some information for selection of the experimental model in pharmacology and pathogenesis study.
8.Prevalence and risk factors of diabetic retinopathy in type 2 diabetic inpatients
International Eye Science 2015;(2):337-339
AlM: To investigate the risk factors of diabetic retinopathy ( DR) in type 2 diabetes patients.METHODS:A total of 380 cases of type 2 diabetes were selected from January 2014 to June 2014, divided into DR group (126 cases) and non-diabetic retinopathy ( NDR) as control group (254 cases), all patients were underwent medical history, physical examinations, laboratory and assistance examinations. The prevalence and single factor and multiple factors of DR were analysed by the logistic regression model.RESULTS: The univariate logistic regression analysis showed that DR was associated with diabetes duration, systolic blood pressure, triglyceride, total cholesterol, low - density lipoprotein, urine protein, intraocular pressure, carotid intima - media thickness, peripheral neuropathy disease was the related risk factors for DR. ln multiple logistic regression analysis, only duration was the related risk factors for DR.CONCLUSlON:The occurrence of DR is the outcome of combined action of multiple factors, duration is an independent risk factor for occurrence of DR.
9.Practice of Bilingual Teaching of Human Parasitology Course
Chinese Journal of Medical Education Research 2006;0(08):-
Promoting bilingual teaching is an important part of reforms in higher education and also a need for training high-quality medical talents.This article discusses practice of bilingual teaching of human parasitology and analyzes the problems in teaching.
10.The Status of Hospice Care Policy in Our Country
Chinese Medical Ethics 2015;(3):402-404
This paper discussed the development of hospice care in our country form five respects , detailed e-laborated our country the main manifestations of lacking hospice medical service policy : relevant policies render fragments;relevant policy interoperability is not strong;Relevant policy lack of financial support;the lack of pub-licity related policy .On this basis , put forward the hospice career development needs of related policies , inclu-ding:set up complete medical security system;establish perfect hospice service classification management mecha-nism;establish and perfect the government funds allocated for hospice care medical services .