1.Abnormal mitochondrial function impairs calcium influx in diabetic mouse pancreatic beta cells.
Fei LI ; D Marshall PORTERFIELD ; Xi-Yan ZHENG ; Wen-Jun WANG ; Yue XU ; Zong-Ming ZHANG
Chinese Medical Journal 2012;125(3):502-510
BACKGROUNDAbnormal insulin secretion of pancreatic beta cells is now regarded as the more primary defect than the insulin function in the etiology of type 2 diabetes. Previous studies found impaired mitochondrial function and impaired Ca(2+) influx in beta cells in diabetic patients and animal models, suggesting a role for these processes in proper insulin secretion. The aim of this study was to investigate the detailed relationship of mitochondrial function, Ca(2+) influx, and defective insulin secretion.
METHODSWe investigated mitochondrial function and morphology in pancreatic beta cell of diabetic KK-Ay mice and C57BL/6J mice. Two types of Ca(2+) channel activities, L-type and store-operated Ca(2+) (SOC), were evaluated using whole-cell patch-clamp recording. The glucose induced Ca(2+) influx was measured by a non-invasive micro-test technique (NMT).
RESULTSMitochondria in KK-Ay mice pancreatic beta cells were swollen with disordered cristae, and mitochondrial function decreased compared with C57BL/6J mice. Ca(2+) channel activity was increased and glucose induced Ca(2+) influx was impaired, but could be recovered by genipin.
CONCLUSIONDefective mitochondrial function in diabetic mice pancreatic beta cells is a key cause of abnormal insulin secretion by altering Ca(2+) influx, but not via Ca(2+) channel activity.
Animals ; Calcium ; metabolism ; Diabetes Mellitus ; metabolism ; physiopathology ; Electrophysiology ; Insulin ; secretion ; Insulin-Secreting Cells ; metabolism ; Male ; Membrane Potential, Mitochondrial ; physiology ; Mice ; Mice, Inbred C57BL ; Mitochondria ; metabolism