1.HID-1 is a peripheral membrane protein primarily associated with the medial- and trans- Golgi apparatus.
Lifen WANG ; Yi ZHAN ; Eli SONG ; Yong YU ; Yaming JIU ; Wen DU ; Jingze LU ; Pingsheng LIU ; Pingyong XU ; Tao XU
Protein & Cell 2011;2(1):74-85
Caenorhabditis elegans hid-1 gene was first identified in a screen for mutants with a high-temperature-induced dauer formation (Hid) phenotype. Despite the fact that the hid-1 gene encodes a novel protein (HID-1) which is highly conserved from Caenorhabditis elegans to mammals, the domain structure, subcellular localization, and exact function of HID-1 remain unknown. Previous studies and various bioinformatic softwares predicted that HID-1 contained many transmembrane domains but no known functional domain. In this study, we revealed that mammalian HID-1 localized to the medial- and trans- Golgi apparatus as well as the cytosol, and the localization was sensitive to brefeldin A treatment. Next, we demonstrated that HID-1 was a peripheral membrane protein and dynamically shuttled between the Golgi apparatus and the cytosol. Finally, we verified that a conserved N-terminal myristoylation site was required for HID-1 binding to the Golgi apparatus. We propose that HID-1 is probably involved in the intracellular trafficking within the Golgi region.
Animals
;
Brefeldin A
;
pharmacology
;
Cell Line, Tumor
;
Cytosol
;
drug effects
;
metabolism
;
Humans
;
Intracellular Space
;
drug effects
;
metabolism
;
Membrane Proteins
;
metabolism
;
Protein Transport
;
drug effects
;
Rats
;
Vesicular Transport Proteins
;
metabolism
;
trans-Golgi Network
;
drug effects
;
metabolism
2.Effects of tetrandrine on cytosolic free calcium concentration in corpus cavernosum smooth muscle cells of rabbits.
Ji-Hong LIU ; Jun CHEN ; Tao WANG ; Bo LIU ; Jun YANG ; Xiao-Wen CHEN ; Shao-Gang WANG ; Chun-Ping YIN ; Zhang-Qun YE
Asian Journal of Andrology 2006;8(4):405-409
AIMTo study the relaxation mechanisms of tetrandrine (Tet) on the corpus cavernosum smooth muscle.
METHODSThe corpus cavernosum smooth muscle cells from New Zealand white rabbits were cultured in vitro. [Ca(2+)](i) was measured by Fluorescence Ion Digital Imaging System, using Fluo-2/AM as a Ca(2+)-sensitive fluorescent indicator.
RESULTSTet (1, 10 and 100 micromol/L) had no effect on the resting [Ca(2+)](i) (P>0.05). In the presence of extracellular Ca(2+) (2.5 mmol/L), Tet (1, 10 and 100 micromol/L) inhibited [Ca(2+)](i) elevation induced by high K(+) and phenylephrine (PE) in a concentration-dependent manner (P>0.05). In calcium free solution containing egtaic acid, Tet (1 and 10 micromol/L) had no inhibitory effects on [Ca(2+)](i) elevation induced by PE (P>0.05). However, Tet (100 micromol/L) inhibited [Ca(2+)](i) elevation induced by PE (P>0.05).
CONCLUSIONTet inhibited the Ca(2+) influx from the extracellular site via voltage-activated Ca(2+) channel and alpha(2)-adrenoceptor-operated Ca(2+) channel. At a high concentration, Tet might inhibit the cytosolic calcium pool release in cultured corpus cavernosum smooth muscle cells. This inhibitory action on [Ca(2+)](i) might be one of the relaxation mechanisms of Tet on the corpus cavernosum smooth muscle.
Alkaloids ; pharmacology ; Animals ; Benzylisoquinolines ; pharmacology ; Calcium ; metabolism ; Cytosol ; drug effects ; metabolism ; Male ; Muscle Relaxation ; Muscle, Smooth, Vascular ; cytology ; metabolism ; Penis ; cytology ; metabolism ; Phenylephrine ; pharmacology ; Potassium Chloride ; pharmacology ; Rabbits
3.Effect of staurosporine induced apoptosis of MCF7/GFP-Bax stable cell line on Bax translocation from cytosol into mitochondria.
Acta Pharmaceutica Sinica 2008;43(4):378-382
To investigate Bax translocation from cytosol into mitochondria induced by staurosporine (STS) in GFP-Bax-tagged MCF7 stable cell line, the viability was measured by MTT method. Bax translocation from cytosol into mitochondria was investigated under the fluorescence microscope. The dose-effect and time-course relationships were also observed and the percentage of GFP-Bax punctuate cells were calculated. Immunofluoresence method was used to observe Bax translocation to mitochondria, Cyt-c release from mitochondria and Annexin V label. The TMRE assay was used to investigate membrane pertential (Deltapsim) and function of mitochondria. Western blotting was used to observe the mechanism of apoptosis induced by STS. The results showed that STS can induce Bax translocation from cytoplasm to mitochondria, Cyt-c release from mitochondria and Annexin V label. The Western blotting analysis presented the inhibitory effect on apoptosis induced by STS of SP600125 which is a specific JNK inhibitor. The study revealed the mechanism of STS induced apoptosis associated with JNK activated pathway.
Anthracenes
;
pharmacology
;
Apoptosis
;
drug effects
;
Cell Line, Tumor
;
Cytochromes c
;
metabolism
;
Cytosol
;
metabolism
;
Humans
;
MAP Kinase Kinase 4
;
antagonists & inhibitors
;
Membrane Potentials
;
drug effects
;
Mitochondria
;
metabolism
;
Protein Transport
;
drug effects
;
Staurosporine
;
pharmacology
;
bcl-2-Associated X Protein
;
metabolism
4.Effects of Buyang Huanwu Decoction on antioxidant and drug-metabolizing enzymes in rat liver.
Xing-Hua FAN ; Wei-Zhou SHI ; Yun-Xiang CHENG ; Xiu-Fen YANG
Chinese Journal of Natural Medicines (English Ed.) 2014;12(6):449-454
AIM:
To study the effect of Buyang Huanwu Decoction (BYHWD) on the antioxidant enzymes and drug-metabolizing enzymes in rat liver.
METHOD:
Following treatment of rats with BYHWD at 6.42, 12.83, or 25.66 g·kg(-1) per day for 15 days, microsomes and cytosols isolated from the liver tissues were prepared by differential centrifugation according to standard procedures. The activities of the antioxidant enzymes and cytochrome b5, NADPH-cytochrome P450 reductase, CYP3A, CYP2E1, UGT, and GST of the rat livers were determined by UV-Vis spectrophotometer.
RESULTS:
The activities of ALT, AST, antioxidant enzymes, and the Hepatosomatic Index in serum were not significantly affected. In cytosols, the activity of CAT was significantly increased at the dosage of 12.83 g·kg(-1), and all the other antioxidant activities and MDA levels were not affected by this treatment. BYHWD had no effect on cytochrome b5, NADPH-cytochrome P450 reductase, CYP3A, and UGT. At the highest dose (25.66 g·kg(-1)), the activity of CYP2E1 was significantly inhibited, and the activities of GST and the level of GSH were increased.
CONCLUSION
BYHWD is safe for the liver, and has the functions of detoxification and antioxidant. Patients should be cautioned about the herb-drug interaction of BYHWD and CYP2E1 substrates.
Animals
;
Antioxidants
;
metabolism
;
pharmacology
;
Catalase
;
metabolism
;
Cytochrome P-450 CYP2E1
;
metabolism
;
Cytosol
;
Drugs, Chinese Herbal
;
pharmacology
;
Glutathione
;
metabolism
;
Glutathione Transferase
;
metabolism
;
Herb-Drug Interactions
;
Inactivation, Metabolic
;
drug effects
;
Liver
;
drug effects
;
enzymology
;
Male
;
Microsomes
;
Rats, Sprague-Dawley
5.Dual effect of oxidative stress on NF-kappaB activation in HeLa cells.
Mi Sun BYUN ; Kye Im JEON ; Jae Won CHOI ; Jae Yong SHIM ; Dae Myung JUE
Experimental & Molecular Medicine 2002;34(5):332-339
Reactive oxygen species (ROS) has been implicated as an inducer of NF-kappaB activity in numbers of cell types where exposure of cells to ROS such as H2O2 leads to NF-kappaB activation. In contrast, exposure to oxidative stress in certain cell types induced reduction of tumor necrosis factor (TNF)-induced NF-kappaB activation. And various thiol-modifying agents including gold compounds and cyclopentenone prostaglandins inhibit NF-kappaB activation by blocking IkappaB kinase (IKK). To understand such conflicting effect of oxidative stress on NF-kappaB activation, HeLa cells were incubated with H2O2 or diamide and TNF-induced expression of NF-kappaB reporter gene was measured. NF-kappaB activation was significantly blocked by these oxidizing agents, and the inhibition was accompanied with reduced nuclear NF-kappaB and inappropriate cytosolic IkappaB degradation. H2O2 and diamide also inhibited IKK activation in HeLa and RAW 264.7 cells stimulated with TNF and lipopolysaccharide, respectively, and directly blocked IKK activity in vitro. In cells treated with H2O2 alone, nuclear NF-kappaB was induced after 2 h without detectible degradation of cytosolic IkBa or activation of IKK. Our results suggest that ROS has a dual effect on NF-kappaB activation in the same HeLa cells: it inhibits acute IKK-mediated NF-kappaB activation induced by inflammatory signals, while longer-term exposure to ROS induces NF-kappaB activity through an IKK-independent pathway.
Cell Nucleus/drug effects/metabolism
;
Cytosol/drug effects/metabolism
;
Diamide/pharmacology
;
Hela Cells/drug effects/metabolism
;
Human
;
Hydrogen Peroxide/pharmacology
;
I-kappa B/drug effects/metabolism
;
NF-kappa B/drug effects/genetics/*metabolism
;
Oxidants/pharmacology
;
*Oxidative Stress
;
Protein-Serine-Threonine Kinases/metabolism
;
Signal Transduction/drug effects
;
Time Factors
;
Transcription, Genetic
;
Tumor Necrosis Factor/pharmacology
6.Effect of ginkgolide B on L-type calcium current and cytosolic Ca2+i in guinea pig ischemic ventricular myocytes.
Zhi-Xiong ZHANG ; Xiao-Yan QI ; You-Qiu XU
Acta Physiologica Sinica 2003;55(1):24-28
With whole-cell variant patch-clamp and laser scanning confocal microscope technique, we examined the effect of ginkgolide B (GB) from ginkgo leaves on L-type calcium current and cytosolic [Ca(2+)](i) in guinea pig ischemic ventricular myocytes. The results showed that under normal conditions, at a test voltage of 0 mV, GB had no significant effect on I(Ca,L); and during ischemia, the peak Ca(2+) current reduced by 37.71%, and the I-V curve of I(Ca,L) was shifted upward. 1 micromol/L GB reversed the change induced by ischemia, a result being significantly different from those of the ishemia group (P<0.05).Under control condition, 0.1,1,10 micromol/L GB decreased intracellular calcium concentration by 10.58%, 17.27% and 16.35% (n=12, 12, 10, P<0.01-0.001), respectively. With perfusion of ischemic solution for 12 min, intracellular calcium concentration increased by 20.15%. After a 12 min-perfusion of ischemic solution containing 1 micromol/L nifedipine or 5 mmol/L NiCl2, intracellular calcium concentration increased by 18.18% (P>0.05 vs ischemia) and 11% (P<0.05 vs ischemia), respectively. After 12 min of perfusion with ischemic solution containing 1 micromol/L GB, intracellular calcium concentration increased by 9.6% (P<0.05 vs ischemia). It is shown that GB could reverse the decrease of I(Ca,L) and partially inhibit calcium overload during ischemia.
Animals
;
Calcium
;
metabolism
;
Calcium Channels, L-Type
;
drug effects
;
Cell Hypoxia
;
Cytosol
;
metabolism
;
Ginkgolides
;
pharmacology
;
Guinea Pigs
;
Heart Ventricles
;
cytology
;
Lactones
;
pharmacology
;
Male
;
Myocardial Ischemia
;
metabolism
;
physiopathology
;
Myocytes, Cardiac
;
drug effects
;
metabolism
;
Patch-Clamp Techniques
;
Plant Extracts
;
pharmacology
7.Effect of Bax translocation on TNFalpha induced apoptosis of human breast cancer MCF7 cells stably expressed hGFP-Bax.
Acta Pharmaceutica Sinica 2007;42(12):1254-1258
Established human breast cancer MCF7 cell line stably expressed GFP-Bax, which was used to investigate Bax translocation from cytosol into mitochondria induced by TNFalpha. Bax translocation from cytosol into mitochondria and DNA fragmentation were investigated under fluorescence microscope. The viability of cells treated with TNFalpha was measured by MTT method. Immunofluoresence method was used to investigate Bax translocation to mitochondria and Cyt-c release from mitochondria. The Dose-effect and time-course relationship were also observed and calculated by the percentage of GFP-Bax punctate cells. The TMRE assay was used to investigate the function of mitochondria. The results showed that Bax translocation from cytosol into mitochondria was associated with apoptosis induced by TNFalpha in hGFP-Bax-tagged MCF7 stable cell line.
Apoptosis
;
drug effects
;
physiology
;
Breast Neoplasms
;
metabolism
;
pathology
;
Cell Line, Tumor
;
Cytosol
;
metabolism
;
Dose-Response Relationship, Drug
;
Green Fluorescent Proteins
;
metabolism
;
Humans
;
Mitochondria
;
metabolism
;
Protein Transport
;
Time
;
Tumor Necrosis Factor-alpha
;
administration & dosage
;
pharmacology
;
bcl-2-Associated X Protein
;
metabolism
8.Recombinant adenovirus-mediated human cytosolic glutathione peroxidase gene transfection protects vascular endothelial cells from oxidative damage.
He-li XIANG ; Wu-jun XUE ; Jun HOU ; Pu-xun TIAN ; Yan TENG ; Xiao-ming PAN ; Xiao-ming DING ; Xin-shun FENG
Journal of Southern Medical University 2006;26(10):1417-1420
OBJECTIVETo study the protective effect of recombinant adenovirus-mediated human cytosolic glutathione peroxidase (hCGPx) gene transfection on vascular endothelial cells ECV304 from oxidative damage.
METHODSpGEM-T Easy Vector containing hCGPx cDNA and recombinant adenovirus shuttle plasmid pACCMV-pLpA were used to construct the shuttle plasmid pACCMV-hCGPx for cotransfection of 293 cells with pJM17, thereby to obtain the recombinant adenovirus AdCMV-hCGPx. Cultured ECV304 cells were transfected with AdCMV-hCGPx for 24, 48 and 72 h, respectively, with the cells transfected with the empty vector serving as control, and hCGPx gene expression was then examined in the transfected cells. The transfected cell viability and apoptotic cell ratio were evaluated after treatment of the cells with H(2)O(2).
RESULTSThe expression ratio of hCGPx gene was significantly higher in the AdCMV-hCGPx-transfected cells than in those with empty vector transfection (P<0.01). The hCGPx gene-transfected cells showed significantly higher viability and significantly lower apoptotic ratio than the control cells following challenge with H(2)O(2)-induced oxidative damage.
CONCLUSIONhCGPx gene transfer mediated by recombinant adenovirus protects the vascular endothelial cells from oxidative damage in vitro, possibly due to the antioxidative and apoptosis-inhibiting effect of hCGPx.
Adenoviridae ; genetics ; Apoptosis ; drug effects ; Cell Line ; Cell Survival ; drug effects ; Cytosol ; enzymology ; Endothelial Cells ; cytology ; drug effects ; metabolism ; Flow Cytometry ; Genetic Vectors ; Glutathione Peroxidase ; biosynthesis ; genetics ; Humans ; Hydrogen Peroxide ; pharmacology ; Oxidative Stress ; Plasmids ; genetics ; Time Factors ; Transfection
9.Molecular mechanism of anti-apoptotic action of survivin in NCI-H446 lung cancer cells.
Yu- Qing CHEN ; Wei LI ; Ji-Hong ZHOU ; Dian-Ming LI ; Xue-Mei XIA ; Li-Nian HUANG ; Bai-Qing LI
Chinese Journal of Oncology 2006;28(6):413-417
OBJECTIVETo investigate cell apoptosis induced by survivin ASODN and clarify the precise mechanism of anti-apoptotic action of survivin.
METHODSCells of lung cancer cell line NCI-H446 were treated with survivin ASODN at different concentrations. The changes of survivin mRNA and protein expression were assessed by RT-PCR and Western blot assay. The apoptosis index (AI) and proliferation index (PI) were determined by flow cytometry (FCM). After 500 mmol/L survivin ASODN treatment, cells were stained with Rh123 to detect changes of mitochondrial membrane potential (deltapsim) by FCM. The concentration of cytoplasmic cytochrome c (cyt-c) was continuously determined by ELISA. Relative activities of caspase-9 and caspase-3 were assessed by colorimetric assay. The expression of caspase-8 protein was measured by Western blot assay. The apoptotic rates of lung cancer cells induced by survivin ASODN with or without mitochondrial permeability transition pole (MPTP) inhibitor CsA treatment were assessed by FCM.
RESULTSDown-regulated survivin mRNA was shown to be in dose-dependent and time-dependent manners. Its maximal effect was achieved at a concentration of 500 nmol/L for 72 h, at which mRNA was down-regulated by 62.7%, the expression of survivin protein in NCI-H446 cells was also obviously decreased. After treatment with survivin ASODN at concentration of 500 mmol/L for 72 h, AI was 48.35%, higher than that of control, lipofectin, NSODN, survivin ASODN 100 mmol/L and 300 mmol/L groups (3.75%, 3.41%, 4.69%, 19.85% and 34.39%, respectively). PI was 24.38%, lower than that of control, lipofectin, NSODN, survivin ASODN100 and 300 mmol/L groups (75.74%, 73.12%, 71.76%, 51.03% and 38.94%, respectively). Deltapsim was decreased in 9.54% of NCI-H446 cells treated with survivin ASODN for 3 h and 97.06% for 24 h. Following it, release of cyt-c from mitochondria to cytosol and activation of caspase-9 and caspase-3 increased significantly. The above mentioned indicators changed with a time-dependent and time diversity relationship. In the presence of CsA, the apoptotic rate of lung cancer cells induced by survivin ASODN was decreased significantly. No up-regrulation and activation in caspase-8 protein was observed.
CONCLUSIONSurvivin inhibits apoptosis via regulation of mitochondrial-dependent pathway. survivin ASODN can not only induce apoptosis but also inhibit cell proliferation through blocking the expression of survivin mRNA and protein.
Apoptosis ; drug effects ; genetics ; physiology ; Caspase 9 ; metabolism ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Cyclosporine ; pharmacology ; Cytochromes c ; metabolism ; Cytosol ; drug effects ; enzymology ; metabolism ; Down-Regulation ; Humans ; Immunosuppressive Agents ; pharmacology ; Inhibitor of Apoptosis Proteins ; Lung Neoplasms ; genetics ; metabolism ; pathology ; Membrane Potential, Mitochondrial ; drug effects ; Microtubule-Associated Proteins ; genetics ; metabolism ; Neoplasm Proteins ; genetics ; metabolism ; Oligodeoxyribonucleotides, Antisense ; genetics ; RNA, Messenger ; biosynthesis ; genetics ; Transfection
10.Rapid increase of cytosolic content of acetyl-CoA carboxylase isoforms in H9c2 cells by short-term treatment with insulin and okadaic acid.
Chang Eun PARK ; Sun Min KO ; Jung Mok KIM ; Moon Young YOON ; Ja Young KIM ; In Sug KANG ; Sung Soo KIM ; Joo Hun HA
Experimental & Molecular Medicine 1998;30(2):73-79
Mammalian acetyl-CoA carboxylase (ACC) is present in two isoforms, alpha and beta, both of which catalyze formation of malonyl-CoA by fixing CO2 into acetyl-CoA. ACC-alpha is highly expressed in lipogenic tissues whereas ACC-beta is a predominant form in heart and skeletal muscle tissues. Even though the tissue-specific expression pattern of two ACC isoforms suggests that each form may have a distinct function, existence of two isoforms catalyzing the identical reaction in a same cell has been a puzzling question. As a first step to answer this question and to identify the possible role of ACC isoforms in myogenic differentiation, we have investigated in the present study whether the expression and the subcellular distribution of ACC isoforms in H9c2 cardiac myocyte change so that malonyl-CoA produced by each form may modulate fatty acid oxidation. We have observed that the expression levels of both ACC forms were correlated to the extent of myogenic differentiation and that they were present not only in cytoplasm but also in other subcellular compartment. Among the various tested compounds, short-term treatment of H9c2 myotubes with insulin or okadaic acid rapidly increased the cytosolic content of both ACC isoforms up to 2 folds without affecting the total cellular ACC content. Taken together, these observations suggest that both ACC isoforms may play a pivotal role in muscle differentiation and that they may translocate between cytoplasm and other subcellular compartment to achieve its specific goal under the various physiological conditions.
Acetyl-CoA Carboxylase/metabolism*
;
Acetyl-CoA Carboxylase/drug effects
;
Animal
;
Cell Differentiation/drug effects
;
Cell Line
;
Cell Membrane Permeability
;
Chromones/pharmacology
;
Cytosol/enzymology*
;
Cytosol/drug effects
;
Digitonin/pharmacology
;
Immunoblotting
;
Insulin/pharmacology*
;
Isoenzymes
;
Morpholines/pharmacology
;
Myocardium/cytology
;
Okadaic Acid/pharmacology*
;
Phosphorylation
;
Rats