1.To evaluate the clearance of cytokines by the technique of continuos filtration of blood in patients suffering from severe intoxicated infected burn
Journal of Practical Medicine 2003;469(12):35-37
71 patients with severe burns, who had had various organ dysfunction were divided randomly into 2 groups: one underwent continuous veno-venous hemofiltration (CVVH), and the other was control group. Results showed a significant decrease of plasma level of cytokine, including IL2, IL6, IL8 and TNF after CVVH (p<0.01). In addition, mortality rate of CVVH group accounted for 38.71%, considerably lower than those of the control group
blood
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Burns
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Cytokines
2.Importance of adipo-vascular axis: from basic research to the clinic.
Chinese Journal of Cardiology 2008;36(12):1138-1141
4.Research progress of platelet bacteriostatic effects.
Wenhua WANG ; Lili XING ; Ting MA ; Jiangcun YANG ; Xucang WEI
Chinese Journal of Cellular and Molecular Immunology 2023;39(9):857-862
Platelets not only have hemostatic function, but can also directly or indirectly recognize pathogenic microorganisms and the signals they produce to capture and destroy them through membrane receptors. They can collaborate with various components of the body's immune system by releasing of intraplatelet particulate matter, cytokines and chemokines to perform bactericidal functions. And it can also play a bactericidal role by swallowing pathogens, releasing antimicrobial proteins and chemokines and activating and enhancing other specialized anti-inflammatory cells bactericidal effect, such as leukocytes and so on. However, the bacteriostatic composition and bacteriostatic mechanism of platelets remain unclear, so attention should be paid to the immune mechanism and bacteriostatic effect of platelets.
Blood Platelets
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Anti-Bacterial Agents/pharmacology*
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Cytokines
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Leukocytes
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Particulate Matter
5.Effect of hyperthermia combined with trauma on serum nitric oxide and mean arterial pressure in rabbits.
Guang-zhong CHEN ; Bing-de LUO ; Hong-qin WANG ; Hui-min ZHAI ; Fei ZOU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2003;21(1):30-32
OBJECTIVETo study the early change of serum nitric oxide (NO) after acute heat exposure with trauma and the effect of NO on mean arterial pressure (MAP), thus to provide theoretical basis for studying the mechanism of NO effect in acute stress.
METHODSThe rabbit model of acute heat exposure combined with trauma was established. The animals were divided into four groups, including control, trauma, hyperthermia and hyperthermia combined with trauma. The levels of NO were measured at different time points: 0 h, 1 h, 2 h and MAP was monitored throughout the whole experiment.
RESULTSThe concentration of NO declined at first and then increased at 1 h or so after acute heat exposure and trauma. The levels of NO in hyperthermia with trauma group at 1 h, 2 h were (42.75 +/- 8.24), (59.54 +/- 9.05) micro mol/L respectively (P < 0.05), while those in control group were (56.63 +/- 3.79) and (55.22 +/- 7.15) micro mol/L, the difference at 1h between two groups was significant (P < 0.05). Under the circumstance of hyperthermia and trauma, the level of MAP declined to the lowest point at 60 - 70 min and then showed a transient rise, after that, the level declined rapidly.
CONCLUSIONSAt the early stage of acute heat exposure and trauma, the concentration of serum NO declined at first and then increased, and had certain relationship with the change of MAP.
Animals ; Blood Pressure ; Cytokines ; biosynthesis ; Hot Temperature ; Male ; Nitric Oxide ; blood ; Rabbits ; Wounds and Injuries ; blood ; physiopathology
6.Association of serum inflammatory cytokines and Resolvin D1 concentration with pathological stage of colon cancer.
Qiulin ZHUANG ; Qingyang MENG ; Qiulei XI ; Guohao WU
Chinese Journal of Gastrointestinal Surgery 2018;21(11):1285-1290
OBJECTIVE:
To investigate the levels of serum inflammatory cytokines and Resolvin D1 (RvD1) and their association with pathological staging of colon cancer.
METHODS:
Clinical data of 50 colon cancer patients (colon cancer group) admitted to the General Surgery Department of Zhongshan Hospital of Fudan University from January to December 2016 and 5 ml of whole blood specimen were collected at admission. During the same period, 50 healthy volunteers were enrolled (healthy volunteer group). Inclusion criteria for the colon cancer group: colon cancer diagnosed by preoperative colonoscopy and pathology; no recent enteral or parenteral nutrition support treatment or use of oral nutrition preparation; age ≤85 years; no surgical contraindications by preoperative evaluation; no history of taking fish oil-related preparations; no radiotherapy or chemotherapy before surgery. Healthy volunteer group enrollment criteria: no history of malignant tumors; no organ with organic lesions detected by the healthy examination center of our hospital; detection indicators in normal reference range; no administration of fish oil-related preparations; age ≤ 85 years. Serum inflammatory factors(IL-1β, IL-6, IL-10 and TNF-α) concentrations were detected by chemiluminescence immunoassay; serum RvD1 concentration was measured by enzyme-linked immunosorbent assay. The levels of inflammatory factors and RvD1 were compared between the two groups, and their associations with TNM staging of colon cancer patients were analyzed.
RESULTS:
There were no significant differences in age, gender and nutrition-related indicators between the two groups (all P>0.05). There were 31 males and 19 females in the healthy volunteer group with age of (61.8±11.6) years. There were 23 males and 27 females in the colon cancer group with age of (65.4±12.4) years. According to the 7th edition of the American Cancer Society TNM staging criteria, 10 cases were stage I, 13 cases stage II, 17 cases stage III, and 10 cases stage IV. Compared with healthy volunteer group, colon cancer group had higher serum IL-1β [(3.89±0.24)×10 μg/L vs.(1.55±0.37)×10 μg/L, t=37.52, P<0.01], higher IL-6 [(129.14±3.07)×10 μg/L vs.(51.46±3.14)×10 μg/L, t=125.08, P<0.01], higher IL-10 [(100.59±8.69)×103 μg/L vs.(27.57±4.77)×10 μg/L, t=52.09, P<0.01] and higher TNF-α [(114.31±4.43)×10 μg/L vs.(41.04±5.27)×10 μg/L, t=75.25, P<0.01], while lower RvD1 [(34.19±1.93)×10 μg/L vs.(77.76±1.02)×10 μg/L, t=140.56, P<0.01], all the differences were statistically significant. Subgroup analysis revealed that concentrations of IL-6, IL-1β, IL-10 and TNF-α gradually increased with the advancement of TNM staging (P<0.01). In stage III, concentrations of IL-6, IL-1β, and IL-10 were the highest, TNF-α concentration was the highest in stage IV. RvD1 concentration gradually decreased with the advancement of TNM staging(P<0.01).
CONCLUSIONS
Compared with healthy volunteers, the levels of serum inflammatory cytokines in colon cancer patients increase significantly while the level of RvD1 decreases significantly. Both are associated with higher TNM stage of colon cancer.
Aged
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Colonic Neoplasms
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blood
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immunology
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pathology
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Cytokines
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blood
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Docosahexaenoic Acids
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blood
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Female
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Humans
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Male
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Middle Aged
7.Recent progress in the diagnosis of neonatal septicemia.
Chinese Journal of Contemporary Pediatrics 2013;15(3):236-241
Neonatal septicemia is one of the major causes of morbidity and mortality worldwide during the neonatal period. It can be classified into two subtypes: early-onset sepsis (EOS) and late-onset sepsis (LOS) depending upon the time of onset. In the western developed countries, group B Streptococcal and Escherichia coli are leading pathogens for EOS, while the most frequent microorganism involved in LOS is coagulase negative Staphylococci, which are different from the domestic data. Clinical manifestations of neonatal septicemia are not specific, so that it is often misdiagnosed. This review describes the progress in diagnostic methods for neonatal septicemia, including blood culture, blood cell counts, cytokine profiles and umbilical cord blood examinations. It provides useful information for early diagnosis and treatment of neonatal septicemia.
Blood Cell Count
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C-Reactive Protein
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analysis
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Calcitonin
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blood
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Cytokines
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blood
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Humans
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Infant, Newborn
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Protein Precursors
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blood
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Sepsis
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blood
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diagnosis
8.Unique association of adiponectin isoforms with serum cytokines and redox molecules in patients with chronic obstructive pulmonary disease.
Hu LIU ; Jin-Sheng LIU ; Jin HUANG ; Liang-Wei ZHONG ; Jian-Ying XU
Chinese Medical Journal 2013;126(17):3383-3384
Adiponectin
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blood
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metabolism
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Cytokines
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blood
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Female
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Humans
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Male
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Oxidation-Reduction
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Protein Isoforms
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blood
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metabolism
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Pulmonary Disease, Chronic Obstructive
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blood
9.Relationship of serum 25-hydroxyvitamin D with obesity and inflammatory cytokines in children.
Ling FENG ; Jin-Rong LI ; Fan YANG
Chinese Journal of Contemporary Pediatrics 2013;15(10):875-879
OBJECTIVETo study the relationship of serum 25-hydroxyvitamin D [25-(OH)D3] level with obesity and inflammatory cytokines in children, and to provide a basis for clinical evaluation of the relationship between vitamin D nutritional status and obesity.
METHODSSeventy-eight children with obesity who visited the hospital between February and June 2012 were selected as subjects. According to baseline data, such as age and sex, 105 children who underwent physical examination in the same period were selected as controls. Fasting venous blood samples were taken to measure serum levels of 25-(OH)D3, interleukin-6 (IL-6), interleukin-8 (IL-8), interferon-γ (IFN-γ), and tumor necrosis factor α (TNF-α).
RESULTSSerum 25-(OH)D3 levels were significantly lower in the obesity group than in the control group (P<0.01). Serum 25-(OH)D3 levels were negatively correlated with BMI (r=-0.462, P<0.01). Patients were further divided, according to their serum 25-(OH)D levels, into vitamin D sufficiency, vitamin D insufficiency, vitamin D deficiency and severe vitamin D deficiency subgroups. There were significant differences in serum IFN-γ levels among the subgroups (P<0.05). There were no significant differences in serum IL-6, IL-8 and TNF-α levels between the subgroups, however (P>0.05).
CONCLUSIONSObese children have lower serum 25-(OH)D3 levels than normal children. Serum 25-(OH)D3 level is negatively correlated with BMI, but has little correlation with inflammatory cytokines levels.
Adolescent ; Body Mass Index ; Calcifediol ; blood ; Child ; Child, Preschool ; Cytokines ; blood ; Female ; Humans ; Inflammation ; blood ; Male ; Obesity ; blood
10.Measurement of the reactive oxygen species and cytokines in the seminal plasma of leukocytospermic patients.
Jing LIU ; Yi-Xing WANG ; Yu-Lun WU ; Xian-Ming QIAN ; Zu-Qiong XIANG
National Journal of Andrology 2003;9(2):103-105
OBJECTIVESTo detect the levels of reactive oxygen species (ROS), superoxide dismutase(SOD) and interleukin 8(IL-8) in seminal plasma of infertile patients, and evaluate the possible relationship between those levels.
METHODSSemen was collected from normal donors (15 cases), infertile men without infection (16 cases), and infertile men with infection (leukocytospermia, 11 cases). The routine analysis of semen was accomplished, and then the levels of IL-8, malondialdehyde (MDA), SOD, and white blood cell (WBC) were examined. The correlative analysis between the level of ROS and other parameters in these populations was made.
RESULTSIn leukocytospermic group, the levels of MDA, WBC, and IL-8 were higher than those in the other two groups (P < 0.001). Significantly positive correlation was observed between IL-8 and MDA (r = 0.852, P < 0.001) and between the levels of IL-8 and WBC.
CONCLUSIONSThese findings suggest that increased oxidative stress in patients with leukocytospermia may cause the increase of IL-8(r = 0.818, P < 0.01). The increased oxidative stress may be due to defect in ROS scavenging system.
Adult ; Cytokines ; blood ; Humans ; Infertility, Male ; blood ; complications ; Male ; Male Urogenital Diseases ; blood ; complications ; Reactive Oxygen Species ; blood ; Semen