1.Effects on the expression of lipopolysaccharide-induced inflammatory cytokines mediated by bovine bactericidal/permeability-increasing protein.
Nan YAO ; Jie BAI ; Xuemei ZHANG ; Ning ZHANG ; Weidong WU ; Wenrong LI
Chinese Journal of Biotechnology 2015;31(2):195-205
Bactericidal/permeability-increasing protein (BPI) can bind to and specifically neutralize lipopolysaccharide (LPS) from the outer membrane of Gram-negative bacteria. In order to evaluate potent LPS-neutralizing activity of bovine BPI, the full-length coding sequence (1 449bp) or 714 bp N-terminal coding sequence (BPI714) of bovine BPI was transfected into mHEK293 cells and the expression of LPS-induced inflammatory cytokines was studied. First, we constructed the lentiviral expression vectors and generated mHEK293 cells stably expressing recombinant bovine BPI or BPI714. Then, we detected the expression of IL-8, IL-1β, TNF-α, NF-κB-1 and NF-κB-2 genes by real-time PCR at 0, 1, 3, 6, 12, 24, 36 and 48 h post of LPS induction in cells with or without recombinant bovine BPI or BPI714 ectopic expression, respectively. In response to LPS, the robust abundance of inflammatory cytokines including IL-8, IL-1β, TNF-α and NF-κB-2 was observed in wild type mHEK293 cells at eachtime point. On the contrary, mRNA abundance of IL-8, TNF-α and NF-κB-2 in transfected mHEK293 cells showed no significant changes at each indicated time point. Our results demonstrated that recombinant bovine full length BPI or BPI714 down-regulated the expression of inflammatory cytokines and revealed that either of bovine BPI or BPI714 was able to inhibit the immune respond stimulated by LPS. This study provides evidence for further investigating the mechanisms and application of BPI/LPS-neutralizing activity and also documents a reliable approach for analysis of the efficacy of antibacterial proteins.
Animals
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Antimicrobial Cationic Peptides
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chemistry
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Blood Proteins
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chemistry
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Cattle
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Cytokines
;
biosynthesis
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HEK293 Cells
;
Humans
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Interleukins
;
biosynthesis
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Lipopolysaccharides
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chemistry
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NF-kappa B
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biosynthesis
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Transfection
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Tumor Necrosis Factor-alpha
;
biosynthesis
2.Effects of Malassezia isolates on cytokines production associated with melanogenesis by keratinocytes.
Fan CUI ; Xiao-Dong SHE ; Xiao-Fang LI ; Yong-Nian SHEN ; Gui-Xia LÜ ; Wei-Da LIU
Acta Academiae Medicinae Sinicae 2007;29(2):196-200
OBJECTIVETo investigate the co-culture of keratinocytes with Malassezia isolates which cause the pityriasis versicolor with different color and to analyze the changes of cytokines associated with melanogenesis.
METHODSThe effects of Malassezia species with different proportions on the growth rate of keratinocytes was assessed with 5 g/L methyl thiazolyl tetrazolium (MTT). Co-culture of keratinocytes and Malassezia species were performed with isolates from hyer- and hypo-pigmentation areas of pityriasis versicolor. The supernatants were collected at different time points, and the changes of basic fibroblast growth factor (b-FGF), endothelin-1 (ET-1), nerve growth factor-beta (NGF-beta), interleukin-1alpha (IL-1alpha), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), stem cell factor (SCF) were recorded. Three control groups were established accordingly.
RESULTSWhen the ratio between keratinocytes and Malassezia species was lower than 1: 10, the growth rate of keratinocytes was not affected by Malassezia (P > 0.05). When the ratio was increased above 1:20, the growth rate of keratinocytes was significantly inhibited by Malassezia (P < 0.01). The secretions of IL-1alpha, IL-6, TNF-alpha, and ET-1 was significantly increased after the co-culture of keratinocytes and Malassezia (P < 0.01), while those of b-FGF, NGF-beta, and SCF had no significant changes (P > 0.05). Compared with the isolates from the hypo-pigmentation area, ET-1 induced by isolate from hyperpigmentation area significantly increased (P < 0.01).
CONCLUSIONWhen Malassezia isolates are co-cultured with keratinocytes, the secretions of cytokines associated with melanogenesis may differ from each other. ET-1 may play certain role in the hyper-pigmentation of pityriasis versicolor.
Cell Proliferation ; Cells, Cultured ; Cytokines ; biosynthesis ; Humans ; Keratinocytes ; cytology ; metabolism ; microbiology ; Malassezia ; isolation & purification ; physiology ; Melanins ; biosynthesis ; Tinea Versicolor ; microbiology
3.Suppressed Production of Pro-inflammatory Cytokines by LPS-Activated Macrophages after Treatment with Toxoplasma gondii Lysate.
Eun Jung LEE ; Yoo Mi HEO ; Jong Hak CHOI ; Hyun Ouk SONG ; Jae Sook RYU ; Myoung Hee AHN
The Korean Journal of Parasitology 2008;46(3):145-151
During Toxoplasma gondii infection, macrophages, dendritic cells, and neutrophils are important sources of pro-inflammatory cytokines from the host. To counteract the pro-inflammatory activities, T. gondii is known to have several mechanisms inducing down-regulation of the host immunity. In the present study, we analyzed the production of proand anti-inflammatory cytokines from a human myelomonocytic cell line, THP-1 cells, in response to treatment with T. gondii lysate or lipopolysaccharide (LPS). Treatment of THP-1 cells with LPS induced production of IL-12, TNF-alpha, IL-8, and IL-10. Co-treatment of THP-1 cells with T. gondii lysate inhibited the LPS-induced IL-12, IL-8 and TNF-alpha expression, but increased the level of IL-10 synergistically. IL-12 and IL-10 production was down-regulated by anti-human toll-like receptor (TLR)-2 and TLR4 antibodies. T. gondii lysate triggered nuclear factor (NF)-kappaB-dependent IL-8 expression in HEK293 cells transfected with TLR2. It is suggested that immunosuppression induced by T. gondii lysate treatment might occur via TLR2-mediated NF-kappaB activation.
Animals
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Cell Line
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Cytokines/*biosynthesis
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Humans
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Inflammation/metabolism
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Lipopolysaccharides/*pharmacology
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Macrophages/*drug effects/*metabolism
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Toxoplasma
4.Effect of hyperthermia combined with trauma on serum nitric oxide and mean arterial pressure in rabbits.
Guang-zhong CHEN ; Bing-de LUO ; Hong-qin WANG ; Hui-min ZHAI ; Fei ZOU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2003;21(1):30-32
OBJECTIVETo study the early change of serum nitric oxide (NO) after acute heat exposure with trauma and the effect of NO on mean arterial pressure (MAP), thus to provide theoretical basis for studying the mechanism of NO effect in acute stress.
METHODSThe rabbit model of acute heat exposure combined with trauma was established. The animals were divided into four groups, including control, trauma, hyperthermia and hyperthermia combined with trauma. The levels of NO were measured at different time points: 0 h, 1 h, 2 h and MAP was monitored throughout the whole experiment.
RESULTSThe concentration of NO declined at first and then increased at 1 h or so after acute heat exposure and trauma. The levels of NO in hyperthermia with trauma group at 1 h, 2 h were (42.75 +/- 8.24), (59.54 +/- 9.05) micro mol/L respectively (P < 0.05), while those in control group were (56.63 +/- 3.79) and (55.22 +/- 7.15) micro mol/L, the difference at 1h between two groups was significant (P < 0.05). Under the circumstance of hyperthermia and trauma, the level of MAP declined to the lowest point at 60 - 70 min and then showed a transient rise, after that, the level declined rapidly.
CONCLUSIONSAt the early stage of acute heat exposure and trauma, the concentration of serum NO declined at first and then increased, and had certain relationship with the change of MAP.
Animals ; Blood Pressure ; Cytokines ; biosynthesis ; Hot Temperature ; Male ; Nitric Oxide ; blood ; Rabbits ; Wounds and Injuries ; blood ; physiopathology
5.Mesenchymal stem cells in hematopoietic regulation--review.
Journal of Experimental Hematology 2006;14(1):187-190
As the progenitor of most cell components in the hematopoietic microenvironment, mesenchymal stem cells (MSC) exhibit self-renewal and multilineage differentiation capacity. Through direct interaction with hematopoietic cells, secreting extracellular matrix and factors, MSC maintain the integrity of hematopoietic microenvironment and regulate hematopoiesis accurately. This review summarized the function of MSC in hematopoietic regulation, such as secretion of cytokines supporting hematopoiesis, MSC expression and adhesion molecules interacting with hematopoietic cells, and supportive effects of transplantation combining MSC with HSC on hematopoietic reconstruction, and its clinical perspectives.
Cell Communication
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Cytokines
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biosynthesis
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Hematopoiesis
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physiology
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Hematopoietic Stem Cells
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physiology
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Mesenchymal Stromal Cells
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physiology
6.Negative regulatory approaches to the attenuation of Toll-like receptor signaling.
Muhammad Ayaz ANWAR ; Shaherin BASITH ; Sangdun CHOI
Experimental & Molecular Medicine 2013;45(2):e11-
Toll-like receptors (TLRs) are pivotal components of the innate immune response, which is responsible for eradicating invading microorganisms through the induction of inflammatory molecules. These receptors are also involved in responding to harmful endogenous molecules and have crucial roles in the activation of the innate immune system and shaping the adaptive immune response. However, TLR signaling pathways must be tightly regulated because undue TLR stimulation may disrupt the fine balance between pro- and anti-inflammatory responses. Such disruptions may harm the host through the development of autoimmune and inflammatory diseases, such as rheumatoid arthritis and systemic lupus erythematosus. Several studies have investigated the regulatory pathways of TLRs that are essential for modulating proinflammatory responses. These studies reported several pathways and molecules that act individually or in combination to regulate immune responses. In this review, we have summarized recent advancements in the elucidation of the negative regulation of TLR signaling. Moreover, this review covers the modulation of TLR signaling at multiple levels, including adaptor complex destabilization, phosphorylation and ubiquitin-mediated degradation of signal proteins, manipulation of other receptors, and transcriptional regulation. Lastly, synthetic inhibitors have also been briefly discussed to highlight negative regulatory approaches in the treatment of inflammatory diseases.
Animals
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Cytokines/biosynthesis
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Humans
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Ligands
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Models, Immunological
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Signal Transduction/*immunology
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Toll-Like Receptors/antagonists & inhibitors/*metabolism
7.Expression and bioactivity of OSF-1 in Pichia pastoris.
Donghong KANG ; Yan WANG ; Hongmei ZHANG ; Xiaoyu FENG ; Wei CAO ; Ping WANG
Chinese Journal of Biotechnology 2014;30(2):274-283
In order to research the biologic activity of osteoblast-stimulating factor 1 (OSF-1), the pPIC9K/osf-1 yeast expression vector was constructed to express and purify OSF-1. Firstly, the osf-1 gene sequence was obtained by artificial synthesis and cloned into Pichia pastoris expression vector pPIC9K to generate pPIC9K/osf-1. The recombinant plasmid was linearized by Sac I and transformed into P. pastoris GS115 by electroporation. Recombinant P. pastoris GS115/ pPIC9K/osf-1 was screened by MD and G418-YPD plates and further identified by PCR. The positive P. pastoris was induced with 1% methanol at 25 degrees C for 96 h. The target protein was analyzed by SDS-PAGE showing a special band about 18 kDa. The target protein was successfully purified from the supernatant of the broth using ion exchange chromatography of SP-Sephadex C-50. The purity of target protein was above 98%. Western blotting appeared a good antigenicity of the purified protein. Bioassay results show that the recombinant protein OSF-1 can promote the differentiation and proliferation of osteoblasts MC3T3-E1. We successfully expressed OSF-1 by recombinant P. pastoris for further development of anti-osteoporosis of research and industrial production of OSF-1.
Blotting, Western
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Carrier Proteins
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biosynthesis
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Chromatography, Affinity
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Cytokines
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biosynthesis
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Electrophoresis, Polyacrylamide Gel
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Electroporation
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Pichia
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metabolism
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Polymerase Chain Reaction
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Recombinant Proteins
;
biosynthesis
8.Expression of Toll-Like Receptors in Verruca and Molluscum Contagiosum.
Ja Kyung KU ; Hyun Jo KWON ; Mi Yeon KIM ; Hoon KANG ; Peter I SONG ; Cheryl A ARMSTRONG ; John C ANSEL ; Hyung Ok KIM ; Young Min PARK
Journal of Korean Medical Science 2008;23(2):307-314
Recent studies indicate that several Toll-like receptors (TLRs) are implicated in recognizing viral structures and instigating immune responses against viral infections. The aim of this study is to examine the expression of TLRs and proinflammatory cytokines in viral skin diseases such as verruca vulgaris (VV) and molluscum contagiosum (MC). Reverse transcription-polymerase chain reaction and immunostaining of skin samples were performed to determine the expression of specific antiviral and proinflammatory cytokines as well as 5 TLRs (TLR2, 3, 4, 7, and 9). In normal human skin, TLR2, 4, and 7 mRNA was constitutively expressed, whereas little TLR3 and 9 mRNA was detected. Compared to normal skin (NS), TLR3 and 9 mRNA was clearly expressed in VV and MC specimens. Likewise, immunohistochemistry indicated that keratinocytes in NS constitutively expressed TLR2, 4, and 7; however, TLR3 was rarely detected and TLR9 was only weakly expressed, whereas 5 TLRs were all strongly expressed on the epidermal keratinocytes of VV and MC lesions. In addition, the mRNA expression of IFN-beta and TNF-alpha was upregulated in the VV and MC samples. Immunohistochemistry indicated that IFN-beta and TNF-alpha were predominately localized in the granular layer in the VV lesions and adjacent to the MC bodies. Our results indicated that VV and MC skin lesions expressed TLR3 and 9 in addition to IFN-beta and TNF-alpha. These viral-induced proinflammatory cytokines may play a pivotal role in cutaneous innate immune responses.
Cytokines/metabolism
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*Gene Expression Regulation
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Humans
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Immunohistochemistry/methods
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Inflammation
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Interferon-beta/biosynthesis
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Keratinocytes/cytology
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Models, Biological
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Molluscum Contagiosum/*metabolism
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Toll-Like Receptor 3/biosynthesis
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Toll-Like Receptor 9/biosynthesis
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Toll-Like Receptors/*biosynthesis
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Tumor Necrosis Factor-alpha/biosynthesis
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Warts/*metabolism
9.The effects of nuclear factor-kappa B p65 antisense oligonucleotides on expression of proinflammatory cytokines in lamina propria mononuclear cells from patients with ulcerative colitis.
Huatian GAN ; Qin OUYANG ; Youqin CHEN ; Feng LIANG
Journal of Biomedical Engineering 2003;20(2):268-272
To investigate if nuclear factor-kappa B (NF-kappa B) p65 antisense oligonucleotides might affect the expression of NF-kappa B p65 and cytokines in lamina propria mononuclear cells(LPMC) from patients with ulcerative colitis (UC). LPMC were isolated from intestinal mucosal biopsy specimens from 3 patients with UC, and cultured with or without NF-kappa B p65 antisense oligonucleotides (5'-GGAACAGTTCGTCCTATGG-3'), missense oligonucleotides (5'-GGAACAGTTCGTCTATGG-3') and dexamethasone. NF-kappa B p65 expression was determined by western blot analysis. The expression of cytokine mRNA was studied by reversal transcription-polymerase chain reaction (RT-PCR). The cytokine levels were measured by enzyme linked immunosorbent assay. The results showed that NF-kappa B p65 antisense oligonucleotides resulted in down-regulation of NF-kappa B p65 expression, blocked the expression of IL-1 beta mRNA and IL-8 mRNA, and strikingly reduced the production of IL-1 beta and IL-8, and these effects were greater than those of dexamethasone in cultured LPMC from patients with UC(P < 0.05). Therefore, the application of NF-kappa B p65 antisense oligonucleotides may serve as a novel molecular approach for the treatment of patients with UC.
Cells, Cultured
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Colitis, Ulcerative
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drug therapy
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pathology
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Cytokines
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biosynthesis
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genetics
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Humans
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Interleukin-1
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biosynthesis
;
genetics
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Interleukin-8
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biosynthesis
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genetics
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Intestinal Mucosa
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cytology
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Monocytes
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drug effects
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metabolism
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NF-kappa B
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biosynthesis
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genetics
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Oligonucleotides, Antisense
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pharmacology
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RNA, Messenger
;
biosynthesis
10.A preliminary study on the changes of expression of PDGF-beta, PDGFR-beta, TGF-beta 1, TGFR, bFGF and its relationship with the wound age in wound healing.
Hui Jun WANG ; Hai Gen RUAN ; Guang Zhao HUANG
Journal of Forensic Medicine 2001;17(4):198-204
OBJECTIVE:
To explore the relationship between the expression change of cytokines and the wound age during the healing process of rats skin wound.
METHODS:
Immunohistochemical and image-analysis methods were performed on vital skin wounds(after incision 0.5-168 h am) and postmortem damage(after incision 0.5-6 h pm).
RESULTS:
The expression of the cytokines PDGF-beta, PDGFR-beta, TGF-beta 1, and bFGF in the epithelial cells was already enhanced since 0.5 h am after damage and their strongest expression reaction was seen at 24-96 h am. In addition, the expression of PDGF-beta, PDGFR-beta, TGF-beta 1 and bFGF was also found in the macrophages and the fibroblasts of the granulation tissue, and the expression changes in the postmortem damage group showed that the skin tissue within 0.5-3 h after incision showed immunohistochemical changes but weakly expression and 3 h thereafter no any change was found.
CONCLUSION
The expression characteristics of the above mentioned cytokines in wound repair should be related to the wound age and it reminds therefore that they may be used as immunohistochemical criteria for accurate determining the wound age.
Animals
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Cytokines/biosynthesis*
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Female
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Fibroblast Growth Factor 2/biosynthesis*
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Male
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Platelet-Derived Growth Factor/biosynthesis*
;
Rats
;
Rats, Wistar
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Receptor, Platelet-Derived Growth Factor beta/biosynthesis*
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Skin/metabolism*
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Time Factors
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Transforming Growth Factor beta/biosynthesis*
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Transforming Growth Factor beta1
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Wound Healing