2.Efficacy and safety of mycophenolate mofetil versus cyclophosphamide in the treatment of Henoch-Schönlein purpura nephritis with nephrotic-range proteinuria in children: a prospective randomized controlled trial.
Hai-Yun GENG ; Chao-Ying CHEN ; Hua-Rong LI ; Juan TU ; Pei-Wei DU ; Hua XIA
Chinese Journal of Contemporary Pediatrics 2021;23(4):338-342
OBJECTIVE:
To study the efficacy and safety of mycophenolate mofetil (MMF) versus cyclophosphamide (CTX) in the treatment of children with Henoch-Schönlein purpura nephritis (HSPN) and nephrotic-range proteinuria.
METHODS:
A prospective clinical trial was conducted in 68 pediatric patients who were admitted to the Department of Nephrology, Children's Hospital Affiliated to Capital Institute of Pediatrics and who were diagnosed with HSPN and nephrotic-range proteinuria from August 2016 to November 2019. The patients were randomly divided into two groups:MMF treatment (
RESULTS:
At months 3, 6, and 12 of treatment, there was no significant difference in the complete remission rate and the response rate between the MMF treament and CTX treatment groups (
CONCLUSIONS
MMF and CTX have similar efficacy and safety in the treatment of HSPN children with nephrotic-range proteinuria.
Child
;
Cyclophosphamide/adverse effects*
;
Humans
;
Immunosuppressive Agents/adverse effects*
;
Mycophenolic Acid/adverse effects*
;
Nephritis/drug therapy*
;
Prospective Studies
;
Proteinuria/etiology*
;
Purpura, Schoenlein-Henoch/drug therapy*
;
Retrospective Studies
3.Therapeutic effect of mycophenolate mofetil or cyclophosphamide in children with Henoch-Schönlein purpura nephritis of different age groups.
Pei-Wei DU ; Yu-Bing WEN ; Chao-Ying CHEN ; Juan TU ; Hua-Rong LI
Chinese Journal of Contemporary Pediatrics 2023;25(11):1113-1117
OBJECTIVES:
To investigate the difference in the therapeutic effect of mycophenolate mofetil (MMF) or cyclophosphamide (CTX) in children with Henoch-Schönlein purpura nephritis (HSPN) of different age groups.
METHODS:
A retrospective analysis was conducted on the clinical data of 135 children with HSPN who were treated with MMF or CTX in the Department of Nephrology, Children's Hospital Affiliated to Capital Institute of Pediatrics, from October 2018 to October 2020. According to the immunosuppressant used, they were divided into two groups: MMF group and CTX group, and according to the age, each group was further divided into two subgroups: ≤12 years and >12 years, producing four groups, i.e, the ≤12 years MMF subgroup (n=30), the >12 years MMF subgroup (n=15), the ≤12 years CTX subgroup (n=71), and the >12 years CTX subgroup (n=19). All children were followed up for at least 12 months, and the above groups were compared in terms of clinical outcomes and the incidence rate of adverse reactions.
RESULTS:
There was no significant difference in the complete response rate between the MMF group and the CTX group after 3, 6, and 12 months of treatment (P>0.05). There were no significant difference in the complete response rate and the incidence rate of adverse reactions between the >12 years MMF subgroup and the ≤12 years MMF subgroup at 3, 6, and 12 months of treatment (P>0.05). The >12 years CTX subgroup had a significantly lower complete response rate than the ≤12 years CTX subgroup at 6 and 12 months of treatment (P<0.05). The >12 years CTX subgroup had a significantly higher incidence rate of adverse reactions than the >12 years MMF subgroup (P<0.05).
CONCLUSIONS
The efficacy and adverse reactions of MMF are not associated with age, but the efficacy of CTX is affected by age, with a higher incidence rate of adverse reactions. CTX should be selected with caution for children with HSPN aged >12 years.
Child
;
Humans
;
Mycophenolic Acid/adverse effects*
;
IgA Vasculitis/drug therapy*
;
Retrospective Studies
;
Cyclophosphamide/adverse effects*
;
Immunosuppressive Agents/adverse effects*
;
Vasculitis/drug therapy*
;
Nephritis/complications*
4.Therapeutic effects of bone marrow transplantation on ovarian injury in mice.
Li-xia LIANG ; Lan CHAO ; Xiao-hui DENG ; Hong-ling YU ; Wen-jun LIU ; Xu-ping WANG
Journal of Southern Medical University 2011;31(9):1534-1538
OBJECTIVETo evaluate the therapeutic effects of bone marrow transplantation (BMT) on ovarian injury induced by chemotherapy in mice.
METHODSForty-eight mice were randomized equally into normal control group (A), cyclophosphamide and BMT group (B), and cyclophosphamide group (C). The mice in groups B and C were treated with intraperitoneal injection of cyclophosphamide at the daily dose of 150 mg/kg for 3 consecutive days, and allogeneic bone marrow cell transplantation was performed in group B. The ovary coefficient and the amount of follicles were compared to evaluate the function of ovaries. For cell tracking, the bone marrow cells were labeled with Hoechst 33342 and detected through fluorescence microscope after transplantation.
RESULTSOn days 21 and 50 after cyclophosphamide treatment, the ovary coefficient and the amount of follicles were significantly lowered in groups B and C (P<0.05), but the reduction was obviously ameliorated in group B (P<0.05). Cell tracking showed the presence of the donor bone marrow cells in the ovaries of the recipients mice after BMT.
CONCLUSIONBMT can improve the ovarian function impaired by chemotherapy in mice.
Animals ; Bone Marrow Transplantation ; Cyclophosphamide ; adverse effects ; Female ; Mice ; Ovary ; pathology ; physiopathology
5.The application of SCGE-KIAS in monitoring of DNA damage in lymphocytes of tumor patients treated with cyclophosphamide.
Shao-Hui CHENG ; Xiao-Hui MA ; Li-Ming BU ; Ning LIU ; Dian-Jun SUN
Journal of Experimental Hematology 2003;11(5):534-537
Single cell gel electrophoresis assay (SCGE), also named as alkaline comet assay, was a simple, rapid and sensitive method to evaluate DNA damage. In this study SCGE technique was used to monitor DNA damage difference in tumor patients caused by chemotherapy, DNA damage distribution frequency and DNA damage characters were analyzed by komet image analysis system (KIAS). The results showed that cyclophosphamide greatly caused DNA damage in lymphocytes of tumor patients. There was significant difference of peripheral blood lymphocyte DNA damage between tumor patients and healthy controls. Tail length of lymphocytes were 33.69 +/- 7.56 micro m, and tail DNA% we re 31.51 +/- 5.4 6% in 10 cancer patients treated with cyclophosphamide, while Tail length were 1 6.2 +/- 1.5 micro m and tail DNA% were 7.46 +/- 1.15% in healthy controls. there was great significant difference on tail length and tail DNA% values between cancer patients and healthy controls (P < 0.01). In conclusion, the successful measurement of DNA damage caused by Cyclophosphamide treatment means that the alkaline comet assay as a valuable tool can be very useful in cancer epideminology study, and also be valuable to evaluate DNA damage status of patients in clinic.
Comet Assay
;
Cyclophosphamide
;
adverse effects
;
DNA Damage
;
Electrophoresis, Agar Gel
;
Humans
;
Lymphocytes
;
drug effects
;
ultrastructure
;
Neoplasms
;
drug therapy
;
genetics
6.Cyclophosphamide-induced rat ovarian damage and expression of gonadotropin-releasing hormone receptor in the damaged ovaries.
Lu LUO ; Dong-zi YANG ; Zhen WANG ; Ya-qin MO ; Qing-xue ZHANG ; Cheng-yu ZHENG
Journal of Southern Medical University 2007;27(11):1714-1717
OBJECTIVETo investigate ovarian follicular damage induced by chemotherapeutic agents and gonadotropin- releasing hormone receptor (GnRHR) expression in the damaged ovaries in rats.
METHODSTwo groups of adult SD rats were subjected to intraperitoneal injection of a single-dose cyclophosphamide and saline, respectively, and 8 weeks later, the ovaries were taken for observing the ovarian damages. The distribution of GnRHR was detected with immunohistochemistry, and RT-PCR was used to determine the expression of GnRHR mRNA in the rat ovaries.
RESULTSMassive primordial follicular loss occurred in the ovaries of rats exposed to cyclophosphamide with also evident stromal ovarian blood vessel damages and focal fibrosis. Both the protein and mRNA expressions of GnRHR were detected in normal rat ovaries, but in rats exposed to cyclophosphamide, the expressions were significantly lowered in the ovaries (P<0.05).
CONCLUSIONLow-level GnRHR expressions in the ovaries of rats with cyclophosphamide exposure suggest microenvironment disturbances in the damaged rat ovaries in advanced stage of chemotherapy.
Animals ; Cyclophosphamide ; adverse effects ; Female ; Humans ; Ovary ; drug effects ; metabolism ; pathology ; Rats ; Rats, Sprague-Dawley ; Receptors, LHRH ; metabolism
7.Effect of cyclophosphamide on murine bone marrow hematopoietic cells and its possible mechanism.
Jie TIAN ; Pei YU ; Wen-Xuan SUN ; Xiao-Yan LI ; Ke-Jing TANG ; Zheng TIAN ; Hai-Yan XING ; Qing RAO ; Min WANG ; Jian-Xiang WANG
Journal of Experimental Hematology 2012;20(6):1316-1321
This study was purposed to investigate the effect of chemotherapeutic drug cyclophosphamide (CTX) on normal murine bone marrow hematopoietic cells, especially on the self-renewal, proliferation and differentiation of bone marrow hematopoietic cells, and possible mechanisms. The CTX-treated mouse model was established by CTX 200 mg/kg, ip. The exact time of complete recovery of hematopoiesis was determined by monitoring the recovery level of differential blood counts and the proportion of LKS(+) cells in bone marrow cells. The function of bone marrow hematopoietic cells such as self-renewal, proliferation and differentiation were assessed by non-competitive and competitive bone marrow transplantation. The potential effect of CTX on senescence of bone marrow hematopoietic cells was analyzed by detecting p16(Ink4a) mRNA relative expression and SA-β-galactosidase (gal) staining. The results showed that the CTX could induce long-term but latent damage to bone marrow hematopoietic cell function and lead to the decrease in competency of bone marrow hematopoietic cells to reconstitute while seemingly permitting a complete recovery. Furthermore, the serial-transplantation model showed that these mice received transplantation of bone marrow hematopoietic cells from CTX-treated mice exhibited a high expression of p16(Ink4a) mRNA and SA-β-gal staining. It is concluded that CTX-induced bone marrow cellular senescence may play an important role in CTX-induced long-term injury to bone marrow hematopoietic cells.
Animals
;
Bone Marrow Cells
;
cytology
;
drug effects
;
Cell Differentiation
;
drug effects
;
Cellular Senescence
;
drug effects
;
Cyclophosphamide
;
adverse effects
;
Hematopoiesis
;
drug effects
;
Mice
;
Mice, Inbred C57BL
8.Oral combination chemotherapy of 5'-deoxy-5-fluorouridine (furtulon) and cyclophosphamide for advanced breast cancer.
Bing-he XU ; Ying-juan ZHANG ; Xiao ZHENG ; Qing WU ; Xiao-ting WU
Chinese Journal of Oncology 2003;25(3):282-284
OBJECTIVETo evaluate the efficacy and safety of the oral combination chemotherapy of furtulon (FTL) and cyclophosphamide (CTX) for advanced breast cancer (ABC).
METHODSFrom Jun 2000 to Jun 2002, eighty-three patients with ABC were treated with the two oral drugs as combined chemotherapy. The mean number of cycles was 5, median number of cycles was 6 (range 2-6).
RESULTSThe overall response rate was 45.8%, The median time to progression was 6 months. The treatment was well tolerated, with related Grade 3 clinical adverse effect being only nausea and vomiting in 2 patients (2.4%) and mild hematologic toxicities.
CONCLUSIONOral combined chemotherapy of FTL and CTX, being convenient and safe, is effective for patients with advanced breast cancer.
Administration, Oral ; Adult ; Aged ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Breast Neoplasms ; drug therapy ; Cyclophosphamide ; administration & dosage ; adverse effects ; Female ; Floxuridine ; administration & dosage ; adverse effects ; Humans ; Middle Aged
9.Clinical analysis of five cases of necrosis of femoral head after acute paraquat poisoning.
Ying-Ping TIAN ; Han-Wen SHI ; Na MENG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2010;28(10):790-791
Adolescent
;
Adult
;
Cyclophosphamide
;
adverse effects
;
Female
;
Femur Head Necrosis
;
etiology
;
Humans
;
Immunosuppressive Agents
;
adverse effects
;
Male
;
Middle Aged
;
Paraquat
;
poisoning
;
Young Adult
10.Cyclophosphamide: Induced lung toxicity in a patient with Wegener's granulomatosis.
Kyoung Ai MA ; Young Il CHOI ; Cheol EOM ; Jin Ho LEE ; Young In CHOI ; Myong Ho HAN ; Kyung Ju PARK ; Hyunee YIM ; Gyu Tae SHIN ; Heung Soo KIM ; Do Hun KIM
Korean Journal of Medicine 2001;61(4):439-443
Lung toxicity associated with cyclophosphamide use is a rare but serious side effect, that may result in a fatal course. However no such cases have been reported in Korea, so clinicians would not be alert to this adverse effect. We recently experienced a woman with Wegener's granulomatosis and idiopathic pulmonary fibrosis. This patient had been administered 12 grams of cyclophosphamide for 4 months. At that time of admission, She felt aggravating dyspnea on exertion for 2 weeks. Her chest x-ray and high resolution CT revealed aggravated reticular opacities and ground glass appearances. Dyspnea was improved and ground glass appearances on HRCT was disappeared after discontinuation of cyclophosphamide. We diagnosed this case as cyclophosphamide-induced pneumonitis and report it with a brief review of the literature.
Cyclophosphamide*
;
Drug-Related Side Effects and Adverse Reactions
;
Dyspnea
;
Female
;
Glass
;
Humans
;
Idiopathic Pulmonary Fibrosis
;
Korea
;
Lung*
;
Pneumonia
;
Thorax
;
Wegener Granulomatosis*