1.Ethanol extract of Angelica gigas inhibits croton oil-induced inflammation by suppressing the cyclooxygenase - prostaglandin pathway.
Sunhee SHIN ; Seong Soo JOO ; Dongsun PARK ; Jeong Hee JEON ; Tae Kyun KIM ; Jeong Seon KIM ; Sung Kyeong PARK ; Bang Yeon HWANG ; Yun Bae KIM
Journal of Veterinary Science 2010;11(1):43-50
The anti-inflammatory effects of an ethanol extract of Angelica gigas (EAG) were investigated in vitro and in vivo using croton oil-induced inflammation models. Croton oil (20 microgram/mL) up-regulated mRNA expression of cyclooxygenase (COX)-I and COX-II in the macrophage cell line, RAW 264.7, resulting in the release of high concentrations of prostaglandin E2 (PGE2). EAG (1~10 microgram/mL) markedly suppressed croton oil-induced COX-II mRNA expression and PGE2 production. Application of croton oil (5% in acetone) to mouse ears caused severe local erythema, edema and vascular leakage, which were significantly attenuated by oral pre-treatment with EAG (50~500 mg/kg). Croton oil dramatically increased blood levels of interleukin (IL)-6 and PGE2 without affecting tumor-necrosis factor (TNF)-alpha and nitric oxide (NO) levels. EAG pre-treatment remarkably lowered IL-6 and PGE2, but did not alter TNF-alpha or NO concentrations. These results indicate that EAG attenuates inflammatory responses in part by blocking the COX-PGE2 pathway. Therefore, EAG could be a promising candidate for the treatment of inflammatory diseases.
Angelica/*immunology
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Animals
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Cell Line
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Cyclooxygenase 1/genetics/*immunology
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Cyclooxygenase 2/genetics/*immunology
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Dinoprostone/genetics/immunology
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Inflammation/drug therapy/enzymology/*immunology
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Interleukin-6/blood
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Macrophages
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Male
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Mice
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Mice, Inbred ICR
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Nitric Oxide/blood
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Phytotherapy/*methods
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Plant Extracts/*pharmacology/therapeutic use
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Plant Roots/immunology
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RNA, Messenger/chemistry/genetics
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Reverse Transcriptase Polymerase Chain Reaction
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Tumor Necrosis Factor-alpha/blood