Objective:To explore the influence ofDan Shou Tang to fetal loss induced by APA.Methods:One hundred of10-week pregnant SD rats were divided randomly into two groups, every group were divided randomly into nourishing kidney and promoting blood flow group A, nourishing kidney group B, promoting blood flow group C, group D-APS model group and group E--blank control group.From the first day ofpregnancy, the rats in group A were given Dan Shou Tang through intragastric administration;the rats in group B were given formula to nourish kidney and rats in group C were given formula to promote blood flow, while group D and group E, as the control were given the corresponding physiological brine through intragastric administration.Then on the eighth and the twelfth day ofpregnancy, all rats ofgroup A, B, C and D were given multi-site subcutaneous injecting ofpurified ACA-IgG or LA-IgG with a dosage of15mg/ml, fats ofgroup E were injected the corresponding physiological brine.On the 15th day ofpregnancy, the rats were killed for samples.Results:Compared with APS model group, fetal absorptivity, ACA and APTT level were dramatically decreased in group A, B and C(P

Objective To investigate the evaluation value of serum microRNA-19b-3p(miR-19b-3p)and microRNA-933(miR-933)levels on cardiac function and prognosis in elderly patients with chronic heart failure.Methods A total of 108 elderly patients with chronic heart failure were selected as study group.According to the New York Heart Society(NYHA)classification,35 pa-tients of the study group were classified into grade Ⅱ,40 patients were classified into grade Ⅲ,and 33 patients were classified into grade Ⅳ.A total of 90 healthy people who underwent physical exami-nation during the same period were selected as control group.Serum miR-19b-3p and miR-933 levels were detected after admission.Cardiac function parameters[left ventricular ejection fraction(LVEF),left ventricular end-diastolic diameter(LVEDD)]were measured by echocardiography.The relationship between serum miR-19b-3p,miR-933 and cardiac function was analyzed by Pearson correlation method.Elderly patients with chronic heart failure were divided into occurrence group(n=34)and non-occurrence group(n=74)according to whether endpoint events occurred within one year of admission.The predictive value of serum miR-1 9 b-3 p and miR-9 3 3 in elderly patients with chronic heart failure was evaluated by receiver operating characteristic(ROC)curve.Multiva-riate Cox regression analysis was used to analyze the prognostic factors of elderly patients with chron-ic heart failure.Results Serum levels of miR-19b-3p and miR-933 in the study group were signifi-cantly lower than those in the control group(P＜0.05).The levels of serum miR-19b-3p,miR-933 and LVEF in patients with cardiac function grade Ⅳ were significantly lower than those in patients with cardiac function grade Ⅲ,grade Ⅱ and healthy population,and LVEDD was larger than that in patients with cardiac function grade Ⅲ,patients with cardiac function grade Ⅱ and healthy popu-lation(P＜0.05).Serum miR-19b-3p level was positively correlated with LVEF and negatively cor-related with LVEDD in elderly patients with chronic heart failure(r=0.554,-0.368,P＜0.001).Serum miR-933 level was positively correlated with LVEF and negatively correlated with LVEDD(r=0.505,-0.410,P＜0.001).The levels of serum miR-19b-3p and miR-933 in the occurrence group were significantly lower than those in the non-occurrence group(P＜0.05).The area under the curve(AUC)of serum miR-19b-3p and miR-933 to predict the prognosis of chronic heart failure in elderly patients were 0.835(95％CI,0.785 to0.885)and 0.843(95％CI,0.790 to 0.890),and the AUC of the combined prediction was 0.901(95％CI,0.851 to 0.951).Serum miR-19b-3p(HR=3.762,95％CI,1.854 to 7.634),miR-933(HR=3.480,95％CI,1.903 to 6.364)and National Institutes of Health Stroke Scale(NIHSS)score(HR=3.047,95％CI,1.837 to 5.051)were the prognostic factors of chronic heart failure in the elderly(P＜0.05).Conclusion Serum miR-19b-3p and miR-933 levels are low in elderly patients with chronic heart failure.The changes of miR-19b-3p and miR-933 levels are closely related to cardiac function and prognosis,which can be used as effective indicators to predict poor prognosis in elderly patients with chronic heart failure.

Objective To investigate the evaluation value of serum microRNA-19b-3p(miR-19b-3p)and microRNA-933(miR-933)levels on cardiac function and prognosis in elderly patients with chronic heart failure.Methods A total of 108 elderly patients with chronic heart failure were selected as study group.According to the New York Heart Society(NYHA)classification,35 pa-tients of the study group were classified into grade Ⅱ,40 patients were classified into grade Ⅲ,and 33 patients were classified into grade Ⅳ.A total of 90 healthy people who underwent physical exami-nation during the same period were selected as control group.Serum miR-19b-3p and miR-933 levels were detected after admission.Cardiac function parameters[left ventricular ejection fraction(LVEF),left ventricular end-diastolic diameter(LVEDD)]were measured by echocardiography.The relationship between serum miR-19b-3p,miR-933 and cardiac function was analyzed by Pearson correlation method.Elderly patients with chronic heart failure were divided into occurrence group(n=34)and non-occurrence group(n=74)according to whether endpoint events occurred within one year of admission.The predictive value of serum miR-1 9 b-3 p and miR-9 3 3 in elderly patients with chronic heart failure was evaluated by receiver operating characteristic(ROC)curve.Multiva-riate Cox regression analysis was used to analyze the prognostic factors of elderly patients with chron-ic heart failure.Results Serum levels of miR-19b-3p and miR-933 in the study group were signifi-cantly lower than those in the control group(P＜0.05).The levels of serum miR-19b-3p,miR-933 and LVEF in patients with cardiac function grade Ⅳ were significantly lower than those in patients with cardiac function grade Ⅲ,grade Ⅱ and healthy population,and LVEDD was larger than that in patients with cardiac function grade Ⅲ,patients with cardiac function grade Ⅱ and healthy popu-lation(P＜0.05).Serum miR-19b-3p level was positively correlated with LVEF and negatively cor-related with LVEDD in elderly patients with chronic heart failure(r=0.554,-0.368,P＜0.001).Serum miR-933 level was positively correlated with LVEF and negatively correlated with LVEDD(r=0.505,-0.410,P＜0.001).The levels of serum miR-19b-3p and miR-933 in the occurrence group were significantly lower than those in the non-occurrence group(P＜0.05).The area under the curve(AUC)of serum miR-19b-3p and miR-933 to predict the prognosis of chronic heart failure in elderly patients were 0.835(95％CI,0.785 to0.885)and 0.843(95％CI,0.790 to 0.890),and the AUC of the combined prediction was 0.901(95％CI,0.851 to 0.951).Serum miR-19b-3p(HR=3.762,95％CI,1.854 to 7.634),miR-933(HR=3.480,95％CI,1.903 to 6.364)and National Institutes of Health Stroke Scale(NIHSS)score(HR=3.047,95％CI,1.837 to 5.051)were the prognostic factors of chronic heart failure in the elderly(P＜0.05).Conclusion Serum miR-19b-3p and miR-933 levels are low in elderly patients with chronic heart failure.The changes of miR-19b-3p and miR-933 levels are closely related to cardiac function and prognosis,which can be used as effective indicators to predict poor prognosis in elderly patients with chronic heart failure.

Objective To establish a new model of indocyanine green (ICG) clearance test combined with total bilirubin actual resident rate (TBARR) for predicting the short-term prognosis of patients with hepatitis B virus-related acute-on-chronic liver failure (HBV-ACLF) treated with artificial liver support system (ALSS) therapy. Methods A retrospective analysis was performed for the clinical data of 136 patients with HBV-ACLF who underwent ALSS therapy in Department of Infectious Diseases, The Affiliated Hospital of Southwest Medical University, from June 2017 to July 2021, and according to the prognosis at 3-month follow-up, they were divided into survival group with 92 patients and death group with 44 patients. Related indicators were measured at the time of the confirmed diagnosis of ACLF, including biochemical parameters, coagulation, indocyanine green retention rate at 15 minutes (ICGR 15 ), and effective hepatic blood flow (EHBF), and related indices were calculated, including Model for End-Stage Liver Disease (MELD) score, MELD difference (ΔMELD), Child-Turcotte-Pugh (CTP) score, total bilirubin clearance rate (TBCR), total bilirubin rebound rate (TBRR), and TBARR. The Mann-Whitney U test was used for comparison of continuous data with skewed distribution between two groups; the chi-square test was used for comparison of categorical data between groups. A binary logistic regression analysis was used to establish a combined predictive model for the prognosis of HBV-ACLF after ALSS therapy. The area under the ROC curve (AUC) was used to compare the accuracy of various models in judging the short-term prognosis of patients with HBV-ACLF after ALSS therapy, and the Z test was used for comparison of AUC. Results There were significant differences between the death group and the survival group in MELD score, ΔMELD, CTP score, ICGR 15 , EHBF, TBRR, TBARR, neutrophil count, percentage of neutrophils, lymphocyte count, platelet count, alkaline phosphatase, gamma-glutamyl transpeptidase, total bilirubin, albumin, prothrombin time, international normalized ratio, prothrombin time activity, prealbumin, fibrinogen, serum sodium, age, and the incidence rate of hepatic encephalopathy (all P < 0.05). The multivariate logistic regression analysis showed that age (odds ratio [ OR ]=1.096, 95% confidence interval [ CI ]: 1.056-1.137, P < 0.001), neutrophil count ( OR =1.214, 95% CI : 1.044-1.411, P =0.012), TBRR ( OR =0.989, 95% CI : 0.982-0.996, P =0.001), TBARR ( OR =1.073, 95% CI : 1.049-1.098, P < 0.001), ΔMELD ( OR =1.480, 95% CI : 1.288-1.701, P < 0.001), CTP score ( OR =2.081, 95% CI : 1.585-2.732, P < 0.001), and ICGR 15 ( OR =1.116, 95% CI : 1.067-1.168, P < 0.001) were independent influencing factors for short-term mortality in patients with HBV-ACLF after ALSS therapy. The binary logistic regression analysis was used to establish four combined predictive models for predicting the prognosis of HBV-ACLF after ALSS therapy, i.e., TBRR-ICGR 15 , TBARR-ICGR 15 , TBARR-ICGR 15 -ΔMELD, and TBARR-ICGR 15 -ΔMELD-age, with an AUC of 0.830, 0.867, 0.900, and 0.917, respectively, and the combined predictive models had a larger AUC than each index alone (age, neutrophil count, TBRR, TBARR, ΔMELD, MELD score, CTP score, and ICGR 15 ), among which the TBARR-ICGR 15 -ΔMELD-age model had the largest AUC. The combined models TBARR-ICGR 15 -ΔMELD and TBARR-ICGR 15 -ΔMELD-age had sensitivities and specificities of > 80%. Conclusion The combined predictive model established by ICGR 15 and TBARR has a good value for in predicting the short-term prognosis of patients with HBV-ACLF after ALSS therapy, and the combined predictive model has a better accuracy than the single model in judging prognosis.