Objective To compare the clinical and pathological differences between IgM nephropathy (IgMN) and IgA nephropathy (IgAN) in children. Methods Clinical manifestations, laboratory examination results, and renal patholog-ical data from 38 children with IgMN and 40 children with IgAN were compared. Results The mean age of onset in IgMN group was younger than that in IgAN group (P<0.05), the mean course before renal biopsy of IgMN group was longer than that of IgAN group (P<0.05), but the incidence of gross hematuria, the level of urinary IgG and albumin, and the incidence of severe glomerular injury were all higher in IgAN group than those in IgMN group (P<0.05). In IgMN group, the level of serum albumin was lower and the urine albumin was higher in the cases with severe glomerular injury than those in the cases without severe glomerular injury (P<0.05);there were more males in those cases with sever glomerular injuries;the incidences of gross hematuria, the level of urine albumin and NAG, and the abnormal basement membrane thickness was higher in the cases with severe tubular injury than those in the cases without severe tubular injury (P<0.05). However, the incidence of severe glomerular injury had no signiifcant difference between the cases with severe and without severe renal tubular injury (P>0.05). In IgAN group, the incidence of proteinuria, RBC casts in tubular, C3 and ifbrinogen deposition, and foot process effacement were higher in the cases with severe glomerular injury than those in the cases without severe glomerular injury (P<0.05); the degree of impairment of renal function, the incidence of severe mesangial cell proliferation, and glomerular sclerosis were more serious in the cases with severe tubular injury than those in the cases without severe tubular injury (P<0.05). Conclusions The clinical and pathological features are different between IgMN and IgAN in children. The renal damage is less in IgMN than that in IgAN children. Different from IgAN children, there is no parallel relationship between tubular and glomerular injury in IgMN children.

Objective To provide an automatical method for quality assurance (QA) of magnetic resonance imaging. Methods Digital imaging and communications in medicine (DICOM) protocol was used to control the process of QA calculation and storage;the region of interest was auto delineated by process. Results The parameters of stability calculated by process reflected the equipment states in a certain extent and avoided the subjectivity which referring to the region of interest. Conclusion Automatical QA method brings the convenience to QA work on aspects of acquiring, analysis and data saving.

ObjectiveTo investigate the anti-tumorigenesis function of rhDCN on the leukemia K562 cells in vitro and analyze the possible mechanism.Methods Exponential phase of K562 cells were transfected with pcDNA3.1(+)-DCN,and PBS,liposome alone,and pcDNA3.1(+) vector were as control groups.Morphology change of K562 cells was detected by Wright stain,and cell proliferation activity was detected by MTT. Cell cycle and apoptosis of K562 cells were assessed by FCM. The expression of apoptosis-related protein,including bcl-xl,Mcl-1 and Bax were detected by Western blot.ResultsWright stain showed that typical apoptotic morphology of K562 cells were observed in DCN transfected group.There were no morphological changes of apoptosis in other groups. MTT method results showed that proliferation inhibition rate of the transfected cells [24 h (16.14±1.08) ％,48 h (14.07±1.01) ％,72 h (20.29±1.19) ％]was higher than that of the other control groups (P ＜ 0.05).FCM results showed that the apoptosis index (20.15±1.31) ％of the DCN transfected group was higher than that of the other groups (P ＜ 0.05),and most of cells arrested in the G0/G1 phase (51.15±0.57) ％ (P ＜ 0.05).Western blot results showed the expression levels of Bax were increased while bcl-xl and Mcl-1 were decreased in pcDNA3.1(+)-DCN/K562 group.ConclusionrhDCN can inhibit the growth of K562 cells and induce the apoptosis.The effect of DCN on bcl-xl,Mcl-1,Bax may play a role in its mechanism.

Our purpose is to introduce and analyze the data quality assurance (DQA) protocol of functional magnetic resonance imaging (fMRI). A water phantom was scanned to get DQA indexes. An fMRI sequence was used to get signal noise ratio (SNR) and Drift, which was calculated from maximum difference ratio of the average signal intensity in the region of interest (ROI) of image serials. The long period application of this method demonstrated that this DQA protocol can reflect imaging performance and the state of stability of the MRI scanner. Some application experience and discussion involved in DQA were also presented here.
Algorithms ; Artifacts ; Artificial Intelligence ; Humans ; Image Processing, Computer-Assisted ; methods ; Magnetic Resonance Imaging ; methods ; Phantoms, Imaging ; standards ; Quality Control

Objective To construct the recombinant plasmid of coxsackievirus group A type 16 (CA16)VP1,express in E.coli and identify expressed product. Methods The CA16 VP1 gene was amplified by PCR,The constructed recombi-nant plasmid pET21b-CA16 VP1 was transformed into E.coli BL21(DE3) for expession and purification,expressed and purified product was identified by SDS-PAGE,Indirect ELISA tested Antigenicity and validity of recombinant proteins CA16 VP1. Results The coxsackievirus group A type 16 VP1 protein was expressed in peokaryotic cells and purified, which showed specific reaction with mice antiserum against CA16 virus,high reactivity with part serums of the elderly population in Taiyuan city. Conclusion CA16 VP1 protein was successfully expressed, had good Antigenicity and va-lidity,which will lay a foundation for study on the CA16 diagnostic kits.