Objective To investigate the distribution and antimicrobial resistance of clinical isolates of Acinetobacter baumannii isolated from our hospitalized patients from 2008 to 2013 , and analyze the correlation be-tween the medication frequency and the drug resistance .Methods The distribution and the drug susceptibility testing for Acinetobacter baumannii isolated from clinical specimens were retrospectively analyzed .The using trend of antibiotics was analyzed by calculation the DDDs .The relation-ship between the drug resistance rate and defined daily dose ( DDDs) was statistically analyzed.Results Acinetobacter baumannii varied in drug resistance,the drug resistance rates to penicillins ,cephalosporins,quino-lones,and carbapenems were high.In 2013,no antibacterial drugs is sen-sitive to Acinetobacter baumannii rate of more than 50%.The resistance rate of Acinetobacter baumannii was negatively correlated with the dosage of piperacillin/tazobactam.Conclusion The resistance rate of Acineto-bacter baumannii is high.And the isolates are multidrug resistant .

OBJECTIVETo compare the contents of chromones in the different isolation and purification procedures from Saposhnikovia divaricata.

METHODThe separation of six chromones was determined simultaneously by HPLC on a Kromasil C18 column at 35 degrees C with the methanol-0.1% phosphoric acid in gradient elution as the mobile phrase. The detection wavelength was set at 254 nm and the flow rate was 1.0 mL x min(-1).

RESULTAll the obtained correlation coefficient of the six components was over 0.9995 and the average recoveries were not less than 97% (RSD <5%), respectively.

CONCLUSIONThe method is simple, accurate with good reproducibility. Results showed the better preparation procedure is water-decoction and ethanol-precipitation with resin separation and silica gel purification.

Apiaceae ; chemistry ; Chromones ; analysis ; isolation & purification ; Plants, Medicinal ; chemistry

OBJECTIVETo establish HPLC fingerprint for the identification of Cassia obtusifolia and compare chemical constituents of the raw and roasted seeds of C. obtusifolia.

METHODChromatographic fingerprint of C. obtusifolia was investigated by RP-HPLC and the gradient elution mode was applied to chromatographic separation. Data were analyzed by fingerprint similarity evaluation software to compare the similarity of the samples for identifying the main chromatographic peaks furthermore.

RESULTHaving compared the HPLC fingerprint of the raw and roasted seeds of C. obtusifolia were compared preliminarily, and 12 main chromatographic peaks were identified.

CONCLUSIONHPLC method can be used in quality control and identification of the raw and roasted seeds of C. obtusifolia.

Cassia ; chemistry ; Chromatography, High Pressure Liquid ; Cooking ; Drugs, Chinese Herbal ; chemistry ; Hot Temperature ; Seeds ; chemistry

OBJECTIVETo compare the contents of diterpenoid pigments in the different processed products of Gardenia jasminoides.

METHODThe separation of Crocin 1, Crocin 2, Crocin 3, Crocetin were determined simultaneously by HPLC on a kromasil C18 column at 35 degrees C with the m methanol-acetonitrile-0.3% formic acid anhydrous in gradient elution as the mobile phrase. The detection wavelength was set at 440 nm and the flow rate was 1.0 mL x min(-1).

RESULTThe obtained linearity of the four components was better over 0.9995 and the average recoveries were 97.77%, 100.05% , 98.40%, 101.02%, respectively.

CONCLUSIONThe method is simple, accurate with good reproducibility. The results showed that the remarkable variation regulations appear among the different processed products.

Carotenoids ; chemistry ; Chromatography, High Pressure Liquid ; Diterpenes ; chemistry ; Drugs, Chinese Herbal ; chemistry ; Gardenia ; chemistry ; Reproducibility of Results

OBJECTIVETo establish methods of RP-HPLC respectively for content-determination of two types of constituents in the Cassia obtusifolia respectively, and tp determine the constituents between the raw and roasted seeds of C. obtusifolia in order to distinguish the difference of those seeds.

METHODHPLC systems consisted of Alltima C18 (4.6 mm x 150 mm, 5 microm), column temperature at 30 degrees C, flow rate of 1.0 mL x min(-1). Two different mobile phases and detection wavelengths: I , ACN-THF-0.1% H3 PO4 (17 : 3:80), 278 nm; II , MeOH-0.1% H3PO4 (79:21), 254 nm. Analyzed and compared the content of the two types of constituents between the raw and roasted seeds of C. obtusifolia.

RESULTThe calibrate curves of 5 constituents were linear (r > 0.999 7). The precision and repeatability were perfect (RSD < 1.6%, 1.8%). The samples were stabile during 24 h.

CONCLUSIONThe study provide a better universal reference for evaluating and controlling the quality of C. obtusifolia pieces.

Cassia ; chemistry ; Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; analysis ; Hot Temperature ; Seeds ; chemistry

OBJECTIVETo explore an effective method for treatment of senile habitual constipation.

METHODSSixty-four cases were randomly divided into a treatment group treated with moving cupping at Hechelu plus Shenque (CV 8) Bazhenxue moxibustion, and a control group with oral administration of Tab phenolphthaleinum, 32 cases in each group. Improvement of constipation in the patients were observed and compared.

RESULTSThe short-term (14 days) clinical cured-markedly effective rate was 84.4% in the treatment group, which was significantly better than 59.4% of the control group (P < 0.05), and there was a significant difference between the two groups in the long-term (2 months) therapeutic effect (P < 0. 01).

CONCLUSIONMoving cupping at the Hechelu combined with Shenque (CV 8) Bazhenxue moxibustion is an effective method for senile habitual constipation.

Acupuncture Points ; Administration, Oral ; Constipation ; Humans ; Moxibustion

OBJECTIVETo explore an effective method for treatment of tennis elbow.

METHODSOne hundred and twenty-eight cases were randomly divided into a treatment group and a control group, 64 cases in each group. The treatment group were treated by electroacupuncture at Hegu (LI 4) and Taichong (LR 3), combined with cake-seperated moxibustion at Ashi points and Shousanli (LI 10) in the affected elbow, and the control group by routine block treatment of 1 mL lidocane and 25 mg prednisone.

RESULTSThe cured rate and the effective rate were 40.6% and 93.7% in the treatment group, and 25.0% and 78.1% in the control group, respectively, with a significant difference between the two groups (P < 0.05).

CONCLUSIONElectroacupuncture combined with cake-separated moxibustion is an effective method for tennis elbow.

Acupuncture Points ; Elbow Joint ; Electroacupuncture ; Humans ; Moxibustion ; Tennis Elbow

OBJECTIVETo establish the HPLC fingerprint method and compare the changes of chemical compositions of the processed products from Sinapis alba.

METHODThe procedure of HPLC analysis was performed on a agilent TC-C18 (2) column at 35 degrees C with the acetonitrile -0.1% phosphoric acid in gradient elution as the mobile phase. The detection wavelength was set at 254 nm, and the flow rate was 1.0 mL x min(-1). The similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (Version 2004 A) was applied to analyze the similarity.

RESULTThe average similarities among the processed products were over 0.96, the standard HPLC fingerprint and five main chromatographic peaks with the isolated compounds was obtained and identified.

CONCLUSIONThe established methods with two solvents are suitable for the HPLC fingerprints determination which elementary elucidate the scientific intensions of breaking the enzyme for glycosides.

Chemistry, Pharmaceutical ; Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; analysis ; Sinapis ; chemistry

OBJECTIVETo compare the contents of iridoid in the different processed products from Gardenia jasminoides.

METHODContents of geniposide and genipin gentiobioside in products were determined simultaneously by HPLC. A kromasil C18 column at 35 degrees C was used with the acetonitrile-0.3% formic acid anhydrous (12:88) as the mobile phrase. The detection wavelength was set at 238 nm and the flow rate was 1.0 mL x min(-1).

RESULTThe obtained linearity of the two components was good with that the relative coefficients (r) were over 0.9994 and the average recoveries were 101.8%, 99.1%, respectively.

CONCLUSIONThe method is simple, accurate with good reproducibility. The results showed contents of two components are difference in the different processed products.

Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; chemistry ; Gardenia ; chemistry ; Iridoids ; chemistry

OBJECTIVETo investigate the chemical constituents of the roasted seeds of Cassia obtusifolia to illuminate the change of its effective components before and after roasted.

METHODCompounds were separated by silica gel chromatography, and their structures were evaluated by spectral analysis and chemical evidence.

RESULTSeven compounds were isolated from the ethanol extract. Their structures were identified as chrysophanol (1), physcion (2), 8-methoxylchrysophanol (3), beta-sitosterol (4), emodin (5), obtusin (6) and obtusifolin-2-O-beta-D-(6'-O-acetyl) glucopyranside (7).

CONCLUSIONCompounds 1-7 were isolated from the roasted seeds of C. obtusifolia for the first time, and compound 7 was a new compound.

Cassia ; chemistry ; Cooking ; Hot Temperature ; Organic Chemicals ; analysis ; isolation & purification ; Seeds ; chemistry