No abstract available.
Culture Media, Conditioned* ; Thymocytes*

OBJECTIVETo study the in vitro embryo culture of Epimedium wushanense and provide scientific basis for large scale production of tissue culture.

METHODCullus and buds were induced from embryo of E. wushanense on a MS medium supplemented with different 2,4-D,6-BA, NAA, IBA.

RESULTThe optimal compositions of medium that induced callus and buds from embryo were the MS medium supplemented with 2,4-D 2 mg x L(-1), IBA 2 mg x L(-1) and NAA 0.5 mg x L(-1) and the MS medium supplemented with IBA 2 mg x L(-1) and 6-BA 0.5 mg x L(-1), respectively. The optimum medium for callus differentiation was MS + 6-BA 1 mg x L(-1) + NAA 0.5 mg x L(-1) + IBA 1 mg x L(-1), and MS +6-BA 1.0 mg x L(-1) + NAA 0.5 mg x L(-1) for shoots proliferation.

CONCLUSIONUsing embryo as explants, the method of induction and culture of E. wushanense was established by the callus and buds, and the embryo of E. wushanense can be quickly propagated.

The aim of the study was to obtain the secondary metabolites in the stem segment of noni and to establish genetic transformation system. The stem segments (no axillary buds) of noni were used as explants to induce the callus, and then to establish the cell suspension system. The factors affecting callus induction and cell suspension were studied. The results showed that the optimal culture medium for induction was MS with 1.0 mg/L 6-Benzylaminopurine (6-BA) and 0.1 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D), and the optimum culture medium for suspension was MS with 1.0 mg/L 6-BA and 0.1 mg/L 2,4-D, 3% sucrose and the pH of 5.85, with the initial inoculation amount of 37.5 g/L, and the speed of 110 r/min and 25±2 °C applying darkness culture. The suspension cells grew well and showed the maximum growth rate. The growth curve of the suspension cells from the stem segment of noni was in "S-typed" trend, and it should be transformed to the fresh medium between 12 and 20 d. During the culture, the pH of the culture medium decreased and then slowly increased, and the optimum pH for the suspension cells culture of callus from noni's stem segments was 4.5-5.0. In this study, the stable cell suspension system of the stem segment of noni was successfully established.
Cell Culture Techniques ; Culture Media ; Morinda ; Sucrose ; Suspensions

OBJECTIVETo study the characteristics of a bioflocculant named MBF7 produced by Penicillum strain HHE-P7 and the effects of cultivation conditions on bioflocculant production.

METHODSThe chemical group in the bioflocculant molecules was shown by Fourier transform infrared (FTIR) spectra, and the average molecular weight of MBF7 was estimated by gel permeation chromatography. The effects of medium components on bioflocculant production and flocculating activity were studied.

RESULTSPhospho-, amino-, hydroxyl, and carboxyl groups were the major fractions of MBF7, and the molecule weight was about 3.0x10(5) Da. In addition, the carbon and nitrogen sources favorable for the bioflocculant production were glucose and yeast extract respectively. When the initial pH of the medium was adjusted to 5.0, high flocculant efficiency could be achieved.

CONCLUSIONThe bioflocculant MBF7 is a new macromolecule with high flocculating efficiency for Kaolin suspension, and could be produced under appropriate culture conditions.

The rabbit keratocytes were cultured to evaluate the effects of L-ascorbic acid(AA), ascorbic acid 2-sulfate(AA-2S), and ascorbic acid 2-phosphate(AA-2P) by measuring cell numbers and 3H-thymidine incorporation. AA at a concentration of 0.05mM enhanced the proliferation of the cells progressively. Addition of 0.1, and 0.5mM AA stimulated the proliferation of cells in the 3rd and 7th day after culture and inhibited in the 15th and 20th day in a dose-dependent manner. AA-2S showed a similar pattern to those of the AA effect, although the inhibitory effect was milder than AA. All concentrations of AA-2P enhanced the proliferation of the cells from the early phase. The effect was prominent in the late phase in a dose-dependent manner. These results suggest that AA-2P is the most stable and the least cytotoxic in the aqueous solution state or culture media and, 0.1-0.5mM concentration of it is best in the promotion of the proliferation of the cultured keratocytes.
Ascorbic Acid* ; Cell Count ; Culture Media

The use of cotton plug as closure of a bacterial culture flask had been reported to have many disadvantages such as inhibitory nature of cotton to certain microbes, chances of contamination during handling and accumulation of used cotton as biological waste. To overcome the disadvantages of cotton plugs, we have developed a new method of capping bacterial culture flasks. In the present study, three sets of experiments were conducted, one was to find out the efficiency of bacterial growth in culture flasks closed by either glass caps or cotton plugs and the second set was to find out the chances of getting contamination of sterile broth closed by either glass caps or cotton plugs and the third set was to find out the evaporation of water in conical flasks closed by glass caps or cotton plug. The results showed that the bacterial cultures closed by glass caps showed better growth with less chance of contamination and evaporation of the culture media. By this method, the bacterial culture work is made very simpler than using cotton plug.
Culture Media ; Glass ; Handling (Psychology) ; Water

Authors are trying to prove the fact that Poly-L-lysine (PLL) might be mixed with alginate to enhance cell-to- matrix adhesion. Before that experiment, the proportion of preadipocytes in cells obtained from rat epididymal fat was detected, and PLL cytotoxicity on preadipocytes was measured. All cells harvested after third passage of culture were differentiated into adipocytes, and there was no decrease in the proliferation of preadipocytes in the culture media under the PLL concentration of 5 microgram/m4. These results suggest that all cells harvested from rat epididymal fat after 3rd passage of culture were preadipocytes and PLL has no cytotoxicity to preadipocytes of rats under the concentration of 5 microgram/mP.
Adipocytes ; Animals ; Culture Media ; Lysine* ; Rats*

One case of fungus infection of the cornea, which led to enucleation, is reported. The fungus of Fusarium species was demonstrated on culture media and microscopic examination. The necessary for awareness of mycotic infections of the cornea, especially in antibiotic or/and cortisone topical treated eyes, is stressed.
Cornea ; Cortisone ; Culture Media ; Fungi ; Fusarium*

OBJECTIVETo study the inhibitory effect of erythritol by contrast to xylitol on growth and acid production of Streptococcus mutans (S. mutans).

METHODSS. mutans were incubated respectively in 0.5%, 1%, 2%, 4%, 8%, 12%, 16% erythritol or xylitol culture medium under anaerobic conditions. The A and pH value of the mediums were measured at 0, 2, 4, 6, 8, 10, 12, 18, 24 hours, following the profile plots by SPSS.

RESULTSThe data of A were higher in 0.5%, 1%, 2% erythritol culture medium than in xylitol culture medium at the same concentration, while lower in 8%, 12%, 16% erythritol culture medium than in xylitol culture medium at the same concentration. The data of pH were lower in 0.5%, 1%, 2% erythritol culture medium than in xylitol culture medium at the same concentration, while higer in 8%, 12%, 16% erythritol culture medium than in xylitol culture medium at the same concentration. It indicated that the growth and acid production of S. mutans were higer in 0.5%, 1%, 2% erythritol culture medium than in xylitol culture medium at the same concentration, while lower in 8%, 12%, 16% erythritol culture medium than in xylitol culture medium at the same concentration.

CONCLUSIONCompared with xylitol, erythritol in low concentration has weaker effort on the growth and acid production of S. mutans, while having stronger effort in high concentration.

To know the effect of subconjunctival injection of liposome encapsulated cytarabine on proliferation of conjunctival fibroblasts, the conjunctiva was isolated at 180 degrees from the injection site 3 days after subconjunctival injection of the normal saline (control), cytarabine, liposome encapsulated cytarabine, and 1 day after injection of cytarabine, and then those were inoculated in the culture media of fibroblasts. In the case of 3 days after injection of cytarabine, there was 49% and 42% inhibition of proliferation of conjunctival fibroblasts compared with the control respectively. Therefore, the authors concluded that the liposome encapsulated cytarabine is effective on inhibition of proliferation of conjunctival fibroblasts and reduces the frequencies of subconjunctival injection compared with the cytarabine itself.
Conjunctiva ; Culture Media ; Cytarabine* ; Fibroblasts* ; Liposomes*