1.Influence of establishment of special nursing clinic on medical compliance behavior of diabetic patients
Cuizhu QIU ; Zefu YANG ; Chunping ZENG ; Shaozhuang LUO ; Qianhua ZHU
Chinese Journal of Practical Nursing 2009;25(24):7-9
Objective To discuss the influence of establishment of special nursing clinic on medical compliance behavior of diabetic patients. Methods We randomly selected outpatients from the endocrine department and special nursing clinic as the control group and the observation group with 100 cases in each group respectively. The condition of medical compliance behavior and blood glucose control was observed and compared in the two groups. Results The patients visiting special nursing clinic were better in medical compliance behavior and blood glucose control. Conclusions Establishment of special nursing clinic for diabetic outpatients can improve the medical compliance behavior, satisfying the requirement for disease knowledge, enhance the shaping of healthy behavior and facilitate the control of disease progress.
2.Astragalin induces autophagy of astrocytes in anterior cingulate cortex of inflammatory pain mice
Jiahong LIN ; Shuhan WANG ; Runheng ZHANG ; Cuizhu YANG ; Yaqi YANG ; Chang ZHOU ; Pei TANG ; Jing LIU ; Yuxin MA
Chinese Journal of Pathophysiology 2023;39(12):2158-2164
AIM:To investigate the effects of astragalin(AST)on activation status of astrocytes and the ex-pression level of autophagy-related proteins in the cortex of the anterior cingulate cortex of mice with a complete Freund's adjuvant(CFA)-induced inflammatory pain model.METHODS:Twenty-four 6-month-old male C57BL/6 mice were ran-domly divided into four groups:control group,saline group,CFA model group and CFA+60 mg/kg AST administration group,and six mice in each group.Mice in the AST administration group received 60 mg/kg AST by intraperitoneal injec-tion on a body weight basis for 21 d.The paw withdrawal threshold in each group of mice was evaluated by the von Frey test.The expression levels of autophagy-related factors LC3,p62,ATG12 and beclin-1,and astrocyte activation were de-tected by multiplex immunofluorescence staining in the anterior cingulate cortex of mice in each group.Western blot was used to measure the levels of autophagy-related proteins LC3,p62,ATG12 and beclin-1 in the anterior cingulate cortex of mice in each group.RESULTS:Behavioural tests showed that AST significantly increased mechanical pain thresholds in CFA mice(P<0.05).The results from multiple immunofluorescent staining showed that AST significantly increased the fluorescence intensity of LC3(P<0.01),ATG12(P<0.01)and beclin-1(P<0.05),attenuated the fluorescence intensi-ty of p62(P<0.05),and inhibited the activation of astrocytes in the anterior cingulate cortex of CFA mice.Western blot results further confirmed that AST significantly increased the expressions of LC3(P<0.01),ATG12(P<0.01),beclin-1(P<0.01),and decreased the expression of p62(P<0.05)in the anterior cingulate cortex of CFA mice.CONCLU-SION:AST relieves CFA-induced inflammatory pain of mice,and its analgesic mechanism may be related to the inhibi-tion of activation of cortical astrocytes in the anterior cingulate cortex and the promotion of autophagy in CFA mice.