Objective To investigate the irfluence of PET/CT positioning on the effect of late-course accelerated hyperfractionated and three-dimensional conformal radiotherapy for stage Ⅲ lung squamous cell carcinoma.Methods 79 stage Ⅲ lung squarnous cell carcinoma patients were enrolled and divided into PET/CT group(n =37 cases,PET/CT positioning) and general CT group (n =42 cases,general CT positioning) according to the way of positioning.The conventional fractionated 3 DCRT( about 40 Gy) was used in previous 2/3 time and hyperfractionated( about 1.5 Gy) was used in later 1/3 time.Both groups used chemotherapy as adjuvant therapy.Full course of the treatment was a month or so.And the clinical efficacy and a variety of adverse reactions of the two groups was observed.Results The total rate of remission in PET/CT group were 73.0％,and 71.4％ in general CT group,and there was no significant difference in the two groups (x2 =1.347,P ＞ 0.05 ).The major radiation injury were radioactive pulmonary injury and tracheal damage,incidence rates were 56.8％and 57.1％ in acute stage,and there was no significant difference in the two groups( x2 =2.178,P ＞ 0.05 ) ; but in late stage 69.0％ in general CT group was significantly higher than 62.2％ in PET/CT group(x2 =4.142,P ＜0.05).Ater followed-up 1 year,the recurrence rate of hillar and mediastinal lymph node in PET/CT group was 43.7％,lower than that of the general CT group( 59.4％ ) ( x2 =4.732,P ＜ 0.05 ).Conclusion PET/CT positioning in late-course accelerated hyperfractionated and three-dimensional conformal radiotherapy for stage Ⅲ lung squamous cell carcinoma could reduce late stage radioactive pulmonary injury and tracheal damage,lower the recurrence rate of hillar and mediastinal lymph node.

Objective To explore the effects of silencing HERC4 on the proliferation,apoptosis,and migration of cervical cancer cell line Hela and the possible molecular mechanisms.Methods Three HERC4-specific small interfering RNAs (siRNAs) were transfected into Hela cells,and HERC4 expression in the cells was examined with Western blotting.CCK-8 assay,annexin V-FITC/PI assay,and wound healing assay were used to assess the effect of HERC4 silencing on the proliferation,apoptosis and migration ability of Hela cells.The expression levels of cyclin D1 and Bcl-2 in the cells were detected using Western blotting.Results Transfection of siRNA-3 resulted in significantly decreased HERC4 protein expression (P＜0.01).HERC4 silencing by siRNA-3 markedly suppressed the proliferation and migration of Hela cells,increased the apoptosis rate (P＜0.01) and reduced the expression levels of cyclin D1 and Bcl-2 (P＜0.01).Conclusion Silencing of HERC4 efficiently inhibits the proliferation,migration,and invasion of Hela cells in vitro,and the underlying mechanisms may involve the down-regulation of cyclin D1 and Bcl-2.

Objective To explore the effects of silencing HERC4 on the proliferation,apoptosis,and migration of cervical cancer cell line Hela and the possible molecular mechanisms.Methods Three HERC4-specific small interfering RNAs (siRNAs) were transfected into Hela cells,and HERC4 expression in the cells was examined with Western blotting.CCK-8 assay,annexin V-FITC/PI assay,and wound healing assay were used to assess the effect of HERC4 silencing on the proliferation,apoptosis and migration ability of Hela cells.The expression levels of cyclin D1 and Bcl-2 in the cells were detected using Western blotting.Results Transfection of siRNA-3 resulted in significantly decreased HERC4 protein expression (P＜0.01).HERC4 silencing by siRNA-3 markedly suppressed the proliferation and migration of Hela cells,increased the apoptosis rate (P＜0.01) and reduced the expression levels of cyclin D1 and Bcl-2 (P＜0.01).Conclusion Silencing of HERC4 efficiently inhibits the proliferation,migration,and invasion of Hela cells in vitro,and the underlying mechanisms may involve the down-regulation of cyclin D1 and Bcl-2.