microRNA (miRNA) is a kind of non-coding small RNA that regulate gene expression at a post-transcriptional level by inhibiting the translation or direct degradation of specific target mRNA.Lots of studies have shown that deregulation of miRNA expression is associated with multisystem tumor.Studies on multiple myeloma (MM) have found that aberrant expression of miRNA regulate MM-cellular processes including cell proliferation,differentiation,apoptosis and drug-resistance.And they can act as diagnostic marker for MM.Some of the miRNA may even function as effective therapeutic targets.This paper reviewed the research progress of the aberrant expression of MM related miRNA in the diagnosis,pathogenesis,drug resistance of MM and its potential application as therapeutic targets.

BACKGROUND: After loss of teeth, the dynamic equilibrium between osteoblast and osteoclast in alveolar bone is destroyed because of systematic and local factors, and residual ridge resorption and atrophy occur irreversibly, which result in the loss of massive alveolar bone. Bone morphogenetic protein-7 (BMP-7) exerts an important effect in the development and traumatic repair of bone and tooth.OBJECTIVE: To investigate the effect of human recombinant bone morphogenetic protein-7 (rhBMP-7) on new bone formation of alveolar ridge and absorption of alveolar ridge.DESIGN: Control experiment.SETTING: Department of Orthodontics, Jinan Stomatological Hospital;Shangdong Academy of Medical Sciences.MATERIALS: This study was conducted at the Shangdong Academy of Medical Sciences from June 2003 to December 2004. Totally 28 New Zealand white rabbits, of either gender, weighing 2 kg, were used in this study.METHODS: Animal extracted wound models were created on the rabbits.The mandibular left and right central incisor of the rabbits were removed.Two carriers containing BMP7 complex (40 μg/each) were implanted into the mandibular right central incisor (experimental group), two empty carriers containing only phosphate buffer solution(PBS) (40 μg/each) were implanted into the mandibular left central incisor (control group). The animals were sacrificed at postoperative 2, 4, 8 and 12 weeks. The specimen from the operated regions were harvested and observed by scanning electron microscope, and calcium content and alkaline phosphatase (ALP) activity were also measured.MAIN OUTCOME MEASURES: ① Result of scanning electron microscope (SEM). ② ALP activity and calcium content.RESULTS: ① The bone wound healing was 4-6 weeks earlier in the experimental group than in the control group . ② The ALP activity was significantly higher in the experimental group than in the control group [week 2:(38.191±5.384, (19.821±2.084) μkat/g;week 4: (160.815±9.669), (126.709±1.634) μkat/g;week 8: (378.892±13.086),(225.212±1.884) μkat/g,P ＜ 0.01]. ③ The calcium was significantly higher in the experimental group than in the control group [week 2:(5.592±0.110), (0.913±0.064) mg/g;week 4: (8.654±0.177), ( 1.702±0.071 ) mg/g;week 8: (25.326±0.287), (5.980±0.145) mg/g,P ＜ 0.01]CONCLUSION: BMP-7, which is expressed in the prokaryocyte, possesses good role in augmenting alveolar ridge and promoting the healing of alveolar wound.

Objective:To observe the effect of moxibusting on heat-sensitive points on pressure sores.Methods:In the treatment group moxibustion on heat-sensitive points was used,and the treatment lasted until the moxa sensation disappeared,one time a day.In the control group,infrared radiation was used for 40 minutes,without avoiding the heat sensitive acupoints,and overlaying the pressure sores with the Mupirocin ointment,covered with sterile gauze once a day.In two groups specific nursing intervention was strengthened diet and moxibustion nursing,and the data was analyzed after one course treatment,which included the cases cured within 7 days.Results:Compared with the 72.4% effective rate in the control group,the effective rate of treatment group was 100%(P

Objectives To analysis clinical pathology of organ speciifc IgA vasculitis (IgA nephropathy) and systemic IgA vasculitis (allergic purpura) of purpura nephritis in children. Methods Clinical and pathological data of hospitalized pediatric patients of IgA nephropathy and purpura nephritis were retrospectively analyzed from June 1993 to November 2014. Results There were 405 patients of IgA nephropathy (256 males and 149 females). The ratio of male to female was 1.7:1. The average age was 10.2±2.8 years. The nephrotic syndrome (31.6%) was the most common clinical type, followed by hematuria and proteinuria (27.9%). There were 548 patients of purpura nephritis, 329 males and 219 females. The ratio of male to female was 1.5:1. The average age was 10.2±3.1 years. The hematuria and proteinuria (61.6%) was the most common clinical type, followed by nephrotic syndrome (21.4%). None of the IgA nephropathy progressed to systemic vasculitis (allergic purpura). Conclusions The causes, onset ages and clinical manifestations of IgA nephropathy and allergic purpura may be consistent or overlap, but none of IgA nephropathy (organ speciifcity IgA vasculitis) progressed to allergic purpura (systemic IgA vasculi-tis). IgA nephropathy might have more renal immune disorder mechanisms involved in its pathogenesis.

Objective: To explore the mechanism of abnormal expression of microRNA155 (miR155) in myeloma drug-resistance to probe the possibility of inhibiting miR155 expression to restore chemotherapy sensitivity and its molecular mechanism in drug-resistant myeloma cells. Methods: Drug-resistant myeloma cell-line RPMI8226/DOX was established by culturing RPMI8226 cells with continuous low concentration and intermittent gradually increasing concentration of doxorubicin in vitro; The levels of miR155 mRNA were measured by qRT-PCR, and both proteins FOXO3a and BCL-2 expressions were detected by Western blot in cell-lines RPMI8226/S and RPMI8226/Dox. RPMI8226/DOX cells were transfected by miR155 inhibitor and mimic using gene transfer method, and then CCK-8 was used to measure proliferation and inhibition ratio, the changes of miR155 expression were detected by RT-PCR. Proteins FOXO3a and BCL-2 were detected by Western blot. Results: Comparing with RPMI8226 cells, the level of miR155 mRNA was obviously up-regulated with the relative expression of 26.860±2.340, together with increased expression of Bcl-2 protein but decreased expression of FOXO3a in RPMI8226/DOX cells. After 72 h treatment with miR155 inhibitor, the inhibition rate of transfection was 64.57%, miR155 expression decreased sharply, the level of FOXO3a expression was upregulated while BCL-2 expression decreased, chemotherapy sensitivity was restored on cell-line RPMI8226/DOX with reversed drug-resistance ratio of 2.518. Conclusions The abnormal expression of miR155 was closely associated with myeloma drug-resistance, targeting inhibition of miR155 expression could restore chemotherapy sensitivity by increasing FOXO3a expression in drug-resistant myeloma cells.

OBJECTIVETo understand the epidemiological characteristics of head injuries in children.

METHODSData was derived from the Chinese National Injury Surveillance System (NISS) in 2014. Method on descriptive analysis was applied to depict general information, injuries events and clinical characteristics of head injuries among children under 18 years of age.

RESULTSA total number of 47 690 cases with child head injuries in 2014 were collected, including 32 542 males and 15 148 females. 43.47% of them were under 1-4 years of age. In October, 06:00 PM appeared the peak time for the injuries to happen. The three leading causes responsible for child head injuries were falls (69.57%), hit by blunt force (14.23%) or road traffic (11.01%). Main locations responsible for the head injuries to happen were:at home (44.98%), at public places (19.65%) or on roads/streets (15.81%). Recreation activates (77.88%), driving (7.32%), sports (5.72%) were the three major activities causing the injuries to take place. Majority of the cases happened unintentionally (95.35%), with bruise (71.69%) or mild injuries (85.27%) and went back home after treatment (90.25%).

CONCLUSIONSIn 2014, child head injuries were seen more in males than in females and mostly occured at home. The leading causes for head injuries would include falls, hit by blunt stuff or road traffic .

Accidental Falls ; statistics & numerical data ; Accidents, Traffic ; statistics & numerical data ; Asian Continental Ancestry Group ; statistics & numerical data ; Automobile Driving ; Child ; China ; epidemiology ; Craniocerebral Trauma ; epidemiology ; Environment ; Female ; Humans ; Male ; Population Surveillance ; Recreation ; Sports ; Transportation ; Wounds and Injuries ; epidemiology

Objective: To analyze and compare the disease burden of falls in Chinese population aged 0-19, in 1990 and 2017. Methods: Indicators including number of deaths, mortality rates, years of life lost due to premature mortality (YLL), years lived with disability (YLD) and disability-adjusted of life years (DALY), on falls, were gathered from the Global Burden of Disease (GBD) 2017 and used to describe the disease burden and corresponding parameters on falls, between 1990 and 2017, in China. Results: In 2017, number of death, YLLs, YLDs, DALYs caused by falls were 5 321, 0.43 million person years, 0.14 million person years and 0.57 million person years respectively, among aged 0-19 group. Rates on standardized mortality, YLLs, YLDs and DALYs on falls were 1.76/100 000, 141.49/100 000, 46.99/100 000, and 188.48/100 000, respectively. The burden of falls decreased with the increase of age. Compared with 1990, disease burden of falls decreased in all age groups, both in male and female, with more seen in the lower age groups. Compared with 1990, the number of deaths, rates on YLLs, YLDs and DALYs caused by falls decreased by 65.08%, 46.63%, 47.38% and 36.33% respectively, in 2017. However, the YLDs rate increased by 73.31% between 1990 and 2017. The ratio of YLLs to DALYs decreased from 90.84% in 1990 to 75.07% in 2017, with a proportion as 17.36%. Conclusion Compared with 1990, although the disease burden of falls among aged 0-19 group showed a decreasing trend, falls still caused serious disease burden for the aged 0-19 group. Research that targeting prevention on falls, should be continued.

Objective: To understand the current situation of road traffic injuries among primary and secondary school students in Mengzi city. Methods: Using the cluster random sampling method, more than 9 000 primary and secondary school students from 20 primary and secondary schools in Mengzi city, were randomly sampled. A questionnaire survey was conducted on the occurrence of road traffic injuries in school students. Results: A total of 9 197 students were surveyed, including 150 from road traffic injuries. The incidence rates of road traffic injuries was 1.63% among students in Mengzi city and 2.85% among junior high school students, with the high incidence in May. The leading places of injuries were village roads (38.00%), city roads (33.33%) and district roads (12.67%), respectively. 26.67% of the traffic injuries occurred on the way to or from schools, with riding on electric bicycle (52.00%) as the major cause and contusion/abrasion (75.33%) appeared as the major signs. Both lower and upper limbs plus multiple parts were accounting for 45.51%, 22.16% and 16.17%, respectively of all the injuries. 70.67% of all the cases with road traffic injuries in children, recovered after treatment. Children with road traffic injuries would stay in the hospital for up to 90 days but spent less than 30 000 Yuan. Conclusion The incidence of road traffic injuries among students in Mengzi city seemed relatively high, with junior high school students reached the highest. Riding electric bicycle appeared as the leading cause for traffic injuries in children in Mengzi city of Yunnan province.

Objective To analyze the burden and changes caused by burns among Chinese population,from 1990 to 2013.Methods Using the G20 data and the China national and subnational data from GBD2013,this study deemed to comprehensively show the burden caused by burns and the change from 1990 to 2013,using the indicators of death and DALY.Results In 2013,the standardized mortality of burns in Chinese was 0.88/100 000,with the standardized DALY rate of burn as 30.58/100 000.The ratio of DALY between males and females was 1.85 ∶ 1.Among the G20 countries,China ranked 16 for the standardized DALY rate,according to the top down order.The highest death rate was seen in the age group of 70 and over,followed by age group of 5,which had the highest DALY rate and followed by the 70 or older age group.In different provinces,Guizhou presented the highest DALY rate (50.24/100 000),with Aomen area the lowest (6.16/100 000).From 1990 to 2013,the burden of burn reduced generally.Both the standardized rates on death and DALY reduced by 68.10％ and 76.95％ respectively,more in females than in males.Standardized rates on death and DALY reduced among all the age groups,with the rates of reduction increasing by age.From 1990 to 2013,the DALY rate decreased in all the provinces.Conclusions The burden of burn decreased in Chinese population during 1990-2013.However,the burden of burn among children,elderly and males were still relatively high that called for closer attention.

Objective To explore the disease burden of road injuries in China.Methods The results of Global Burden of Disease 2013 including death rate,disability-adjusted of life years (DALY),years of life lost due to premature mortality (YLL),years lived with disability (YLD),were used to describe the burden caused by road injuries in 2013 and the trends from 1990 to 2013,in China.Results In 2013,there were 313 676 deaths caused by traffic accidents in China.Death rate,rates on DALY,YLL and YLD were 22.52 per 100 000,1 076.54 per 100 000,971.21 per 100 000 and 105.34 per 100 000,respectively.Rates on deaths,YLL and YLD appeared higher in males,pedestrians than in females and other types of road travelers.Burden of injuries caused by traffic accidents was seen higher in those aged 15 to 49-year-old.From 1990 to 2013,the overall death rate on road injuries increased by 0.54 per 100 000 in China,with an increase of 2.34 per 100 000 and 0.81 per 100 000,respectively in males and pedestrians.The rates on DALY,YLL and YLD decreased by 164.21 per 100 000,115.06 per 100 000 and 49.06 per 100 000,respectively.Conclusions During the past 20 years,achievements had been made on road injury prevention and control,with the decrease of disease burden caused by road accidents.Males,young adults and pedestrians should be called for more attention to prevent road injuries.