An electrogenerated chemiluminescence ( ECL) sensor was fabricated by employing ruthenium complex polymer as ECL signal and ionic liquid as binding reagent and a sensitive ECL method for the determination of heroin was developed. The ECL sensor was prepared by thoroughly mixing ruthenium complex polymer,graphite powder and ionic liquid,which shows good electrochemical and ECL behaviors. The detection limit for tripropylamine (TPA) was one order of magnitude lower than that observed at the paraffin modified carbon paste electrode. ECL intensity of the fabricated sensor was greatly enhanced in the presence of heroin and a sensitive ECL method was proposed for the determination of heroin based on the enhancement ECL. The ECL intensity was linear with the concentration of heroin in the range from 2. 0 ×10~(-9) mol/L to 2. 0×10~(-5) mol/L and the detection limit was 8×10~(-10) mol/L. The ECL sensor exhibited a long-term stability,fine reproducibility with relative standard deviation less than 5% for 5. 0×10~(-9) mol/L heroin in 60 continuous determinations. The developed method allows the detection of heroin in a serum sample with recovery in 94% -101%.

Objective To determine the value of detection of H ras oncogene mutation in urine exfoliated cells as clinical indicator of tumor presence, recurrence and stage.Methods Point mutation at codon 12 of H ras gene was assayed by polymerase chain reaction followed by analysis of single strand conformation polymorphism in urine exfoliated cells from 48 patients with transitional cell carcinoma before operation and 28 patients with non urothelial cancer or normal individuals. The mutation was further confirmed by dideoxy mediated chain termination method of DNA sequencing. Cytology analysis was carried out simultaneously. Bladder tumor specimens were obtained from 48 patients during operation, and histologically elevated for tumor content and grading.Results 48%(23 of 48) of the patients were detected by their aberrant band in SSCP. All aberrant bands displayed a mutant H ras sequence, where 15% (7 of 48) of the patients displayed, apositive cytological analysis. Analysis of abnormalities with tumor stage revealed that the greater detection of high pathological stage (Ⅲ Ⅳ) compared with low stage (Ⅰ Ⅱ) was related to the recurrence of transitional cell carcinoma.Conclusion Our results suggest that the detection of H ras mutations may be of clinical value in the detection of TCC.

Objective:To assess the validity of ultrasonic surveillance of percentage change in the levator ani hiatal antro posterior diameter (LHap) in the diagnosis of pelvic floor muscle dysfunction.Methods:Two hundred and forty seven women suspected to have pelvic floor disorder related symptoms from January to December 2017 were enrolled. Digital palpation of the puborectalis muscle using modified Oxford score grading system (MOS) was performed.Women with MOS point of 0, 1, 2, or 3 were defined as having low pubic floor muscle contraction (LPFMC), and those with MOS point of 4 or 5 were defined as having normal pubic floor muscle contraction (NPFMC). Then ultrasound measurement of LHap diameter at rest, at maximum contraction, and at the maximum Valsalva were performed in all women to calculate the percentage decrease on contraction (PDC%) and percentage increase on Valsalva (PIV%). Statistical analysis was performed to test the significance of differences in PDC% and PIV% between the LPFMC group and NPFMC group, and the ROC curve analysis was performed to evaluate the validity of using PDC% and PIV% for predicting LPFMC.Results:Compared with the NPFMC group, the PIV% of LPFMC group was significantly larger [(6.07±4.20)% vs (11.29±10.49)%, P<0.001], and the PDC% was significantly smaller [(31.36±3.34)% vs (17.66±10.82)%, P<0.001]. A cut-off of PIV%>5.19% predicted LPFMC with sensitivity 71.43%, specificity 57.89%, and the area under the ROC curve was 0.69. A cut-off of PDC%<25.37% predicted LPFMC with sensitivity 66.39%, specificity 97.37%, and the area under the ROC curve was 0.84. To diagnose LPFMC by the combination of PIV >5.19% and PDC%<25.37%, the sensitivity was 84.55%, the specificity was 55.00%, and area under ROC was 0.70. Conclusions:Ultrasonic measurement of percentage change in the LHap diameter is valuable for the diagnosis of pelvic floor muscle dysfunction.