Objective To investigate the effects of uterine malformation on the pregnancy and delivery, and to accumulate experience of uterine malformations obstetric clinical diagnosis and treatment of perinatal. Methods The clinical data of 4 273 cases of pregnant women were retrospectively analyzed. Of them,45 patients with uterine malformation. The effects of uterine malformation on the pregnancy and delivery were evaluated. Results The ratio of development of premature delivery, premature rupture of membranes, abnormal fetal position, retained placenta and perinatal complications in uterine malformation pregnancy group was significantly higher than normal uterine pregnancy group(P＜0.05).The ratio of cesarean section in uterine malformation group also significantly higher than the yield of normal uterine pregnancy(82.2%vsl6.93% ,P＜0.05). Conclusion Pregnant women with uterine malformations had more obstetric complications, higher operative delivery rate, the uterine malformation was high risk factor for the pregnancy,clinical attention should be paid.

Objective:To investigate the differential expression of VEGF165b in the renal cell carcinoma (RCC) and normal renal tissues and its role in the development of RCC. Methods: S-P immunohistochemistry was used to detect the expression of VEGF165b protein in 30 specimens of paraffin-embedded RCC tissues and 29 specimens of paraffin-embedded normal renal tissues. RT-PCR was performed to detect the expression of VEGF165b mRNA in 32 specimens of fresh RCC tissues and 30 specimens of fresh normal renal tissues. Results: Among 29 of normal renal tissues,28 specimens had positive expression of VEGF165b protein,with the positive rate of 96.55%(28/29) that was significantly higher than 20.00%(6/30)in RCC tissues(P

Objective:To investigate the differential expression of VEGF165b in the transitional cell carcinoma of the bladder (TCCB) and normal bladder tissues and its role in the development of TCCB. Methods: S-P immunohistochemistry was used to detect the expression of VEGF165b protein in 38 specimens of paraffin-embedded TCCB tissues and 36 specimens of paraffin-embedded normal bladder tissues. RT-PCR was performed to detect the expression of VEGF165b mRNA in 55 specimens of fresh TCCB tissues and 43 specimens of fresh normal bladder tissues. Results: Among 36 of normal bladder tissues, 35 specimens had positive expression of VEGF165b protein, with the positive rate of 97.22% (35/36) that was significantly higher than 21.05% (8/38) (P

Objective To investigate the influence and treatment of hypertensive disorder complicating pregnancy(HDCP).Methods 178 HDCP cases are selected as observation group,and 178 normal patients as comparison group.Results Fetal growth restriction was 25 cases in disease group and 1 case in normal group(P＜0.01);20 cases are fetal growth restriction in 77 cases with severity pre-eclampsia,which had notability discrepancy compared with mild pre-eclampsia(P＜0.01).Premature delivery was 21 cases(25.33%)in severity pre-eclampsia,which had significant diffcrence.When compared with the rest three groops(P＜0.01).When fetal age is equal to or above 37 weeks,the livability reached to 100%(P＜0.01).Conclusion We should prevent transformation actively,avoid pre-eclampsia and complication to severe pre-eclampsia of mother and baby.

Objective To improve th current teaching situation,increase clinical teaching quality in order to ensure the nursing students to adapt to new nursing model and social development.Methods The main problems existing in clinical nursing teaching of our country was analyzed,furthermore countermeasures were put forward.Results At present clinical nursing teaching of our country lacked perfect management system,teacher resources were weak,teaching time was not reasonably arranged,training of comprehensive quality for nursing students was lack.Conclusions Through analysis of current situation of clinical nursing teaching,we propose the corresponding ponder,guarantee the clinical nursing teaching quality and do fully preparatory work for training of nursing reserve forces with high quality.

Objective:This paper is aimed at exploring the correlations between thinking style and mental health in university students from the perspective of culture.Methods:800 university students were tested by the questionnaires of the Thinking Style and SCL-90.Results:①The thinking style scores of students with high SCL-90 were significantly different from those of students with low SCL-90 in covariant thinking(t=3.934,P

AIM: To demonstrate the effect of mitochondrial ATP-sensitive potassium channel opener,Diazoxide,on glutamate uptake activity of astrocyte. METHODS: Primary astrocytic cultures were prepared from cerebral cortices of rats within 3 days old.Glutamate uptake activity of astrocytes was measured using isotopic techniques to detect intracellular -labeled D,L-glutamate concentration.RESULTS: Diazoxide increased -glutamate uptake in a concentration-dependent manner,and could significantly reverse MPP~+-induced glutamate uptake inhibition.The above effects of Diazoxide were blocked by 5-hydroxydecanoate,a selective mitochondrial ATP-sensitive potassium channel blocker.CONCLUSION: Diazoxide increase the activity of glutamate transporters in astrocytes by opening mitochondrial ATP-sensitive potassium channel.

Cyclin E is expressed starting from the middle G1 phase of the cell cycle,and is accumulated in the G1/S boundry.Cyclin E binds to and activates the cyclin-dependent kinase CDK2.Cyclin E-CDK2 complex initiates a cascade of events that leads to DNA replication by phosphorylating its substrates,such as Rb,CDC6,NPAT and P107,etc.Additionally,cyclin E plays an important role in the regulation of genomic stability,spindle-organizing structure and centrosome cycle.Cyclin E expression is trans-activated by members of the transcription factor E2F family and degrades via the ubiquitin-proteasome pathway.At the same time,it is also negatively regulated by the CIP/KIP proteins.Cyclin E highly expressed in the initiation and progression of different human cancers,such as breast cancers,lung cancers,leukemia,lymphomas and others.

The application of electron impact, chemical ionization , fast atom bombardment, electro-spray ionization, matrix-assisted laser desor ption ionization and tandem mass spectrometry in the structural analysis of sacc harides was reviewed, 53 papers were cited.