Objective To investigate the expression of CA242 and CD_(44v6) protein in gastric cancer (GC) and its clinical significance. Methods Immunohistochemical staining was used to detect the expression of CA242 and CD_(44v6) protein in 120 cases of GC, 30 cases of gastric epithelial dysplasia and 20 cases of normal gastric mucosa. The pathological feature of GC and clinical follow-up data was also analysed. Results The positive rate of CA242 and CD_(44v6) was 79.2 % and 81.7 %, respectively in primary GC. The positive rate of CA242 and CD_(44v6) in GC was higher than that in epithelial dysplasia and normal gastric mucosa (P <0.05). The expression of CA242 and CD_(44v6) was closely related to infiltration of serosa, metastasis of lymph node and prognosis of patients (P <0.05). Conclusion These data suggest that the expression of CA242 and CD_(44v6)is closely related to carcinogenesis, metastasis and survival rate in GC. CA242 and CD_(44v6) may be prognostic indicators of GC.

Objective To investigate the relationship between the expression of tumor suppressor gene PTEN and p16 protein and the biologcal behaviors of cholangiocarcinomas.Methods The expression of PTEN and p16 protein in 43 of cholangiocarcinoma tissues and 10 chronic cholangitis tissues were investigated by immunohisto-chemical technique.The relationship between expression of PTEN and p16 protein and the clinicopathological parameters of cholangiocarcinoma was analyzed. Results The positive expression rate of PTEN and p16 protein in cholangiocarcinoma was 39.5% and 44.2%,respectively.The expression of PTEN and p16 protein were positively relatad with the TNM staging,differentiation degree and metastasis of cholangiocarcinoma (P

Objective:To evaluate pathogenesis of nasal polypsis.Method:To detect HPV in 26 pathological samples of 13 cases by polymerase chain reaction(PCR).Ten cases of normal nasal mucous were used as control.Result:HPV-DNA was positive 12 cases(92.3%) in 13 cases of the first surgical operation by PCR.HPV-DNA was positive 5 cases(38.5%)in 13 cases of the second surgical operation by PCR.The most HPV type was HPV6.HPV-DNA was negative in 10 cases of control.Conclusion:The results showed that HPV plays an etiologic role in the development of nasal polypsis.

AIM: To investigate the clinical effect and side-effects of paroxetine in the treatment of generalized anxiety (GA). 　METHODS: Ninety patients who met Chinese classification and diagnostic criteria of mental disorders, 2nd Rev Ed (CCMD-2-R) criteria for GA were randomly divided into paroxetine group of 30 patients (M 12, F 18; age 40 a±s 13 a) which was treated with paroxetine 20-40 mg, po, qd or bid, benzodiazepines group of 30 patients (M 13, F 17; age 37 a±13 a) among them, 16 patients were treated with alprazolam 0.4-0.8 mg, po, bid or tid, 14 patients were treated with clonazepam 1-2 mg, po, bid or tid and placebo group of 30 patients (M 11, F 19; age 37 a±13 a) which was treated with placebo 1 tablet, tid for 12 wk. Effects were evaluated with Hamailton anxiety scale (HAMA), self-rating anxiety scale (SAS), clinical global impression scales (CGI) and treatment emergent symptoms scale (TESS), before and after the wk 2,4,8,12 of treatment.　RESULTS: The excellent response rates of paroxetine group and benzodiazepines group were 90 % and 50 % (P<0.01). The side-effects of paroxetine group were dry mouth, constipation, nausea, but those were less than that of benzodiazepines group. 　CONCLUSION: The study suggests that paroxetine is an effective drug for the long treatment of GA and the side-effect is less.

Objective To investigate the clinical effect and the safety of paroxetine in the treatment of elderly patients with chronic primary insomnia. Methods Ninety elderly patients who met Chinese Classification and Diagnostic Criteria of Mental Disorders, 2nd Rev Ed, (CCMD 2 R) for elderly patients with chronic primary insomnia were randomly divided into paroxetine group (30 cases), alprazolam group (30 cases), and placebo group (30 cases) for 12 weeks as a course. Effects were evaluated with Pittsburgh Sleep Quality Index (PSQI) and treatment emergence symptoms scale (TESS) before and after the 4th, 8th, 12th week of treatment. Results The excellent response rate of paroxetine group and alprazolam group were 83% and 53% respectively( P

AIM: To compare the effects between topiramate and slow-release sodium valproate in treating refractory epilepsy. 　METHODS: Topiramate group of 39 patients (M 21, F 18; age 28 a± s 20 a) was compared with sodium valproate group of 41 patients (M 22, F 19; age 27 a±17 a) in antiepileptic effect of refractory epilepsy. Adult's and children's dosages of topiramate were increased gradually about 200 mg*d-1 and 4 mg*kg*d-1 respectively during about 2 mo, po, bid, for 6 mo as a course. Adult's dosage of slow-release sodium valproate was 0.5-1 g*d-1, and children's was increased gradually to total dosage: 15-30 mg*kg*d-1, po, qd or bid (morning or morning and noon), for 6 mo as a course. Effects were analysed between these two drugs after treatment 4 and 6 mo.　RESULTS： Simple and complex partial seizures with or without secondary generalized seizure, in topiramate group were much more improved than these in sodium valproate group 6 mo after treatment. Four patients of topiramate group appeared temporary adverse reactions of central nervous system, such as tiredness, sleepiness and distraction, but one patient of sodium valproate group had severer decreased function of bone marrow.　CONCLUSION: Topiramate is one of effective antiepileptic drugs and superior to slow-release sodium valproate. There are the apparent absence of any effects of topiramate on the bone marrow and on indexes of liver and kidney.

Objective To investigate the effects of telmisartan on the blood glucose, blood lipid, blood insulin, and insulin resistance in the hypertensive patients with dyslipidemia, and also its effect on controlling blood pressure. Patients and Methods A total of 96hypertensive patients (34 females, 62 males) with dyslipidemia were included (mean age 51.2±9.6, range 42-65 years). Patients were randomized to receive either telmisartan 80 mg/day (n=46) or enalapril 10 mg/day (n=50) for 6 months. The levels of blood pressure (BP), heart rate (HR), and biochemical data were measured before therapy and at the end of the 3-month treatment and 6-month treatment, respectively. Meanwhile, insulin resistance was evaluated by using a homeostasis model assessment of insulin resistance (HOMA-IR) and insulin sensitivity (HOMA-IS). Results In the telmisartan group, the mean blood pressure was obviously lower than that of pre-therapy (P＜0.05), and the levels of triglyceride (TG), HOMA-IR, and HOMA-IS were all obviously lower than those of pre-therapy and of the enalapril group at the end of the 3-month-treatment period (P＜0.05). After 6 months of treatment, the levels of TG, HOMA-IR, and HOMA-IS in the telmisartan group were significantly lower in comparison with those of pre-therapy, the enalapril group (P＜0.01), and 3-month-treatment (P＜0.05). Post-prandial12 hour blood glucose (P2HBG) in the telmisartan group decreased significantly after 6-month treatment compared with that of pre-therapy and the enalapril group (P＜0.05). The level of high density lipoprotein (HDL) cholesterol was significantly higher after 6-month treatment in the telmisartan group than with pre-therapy and the enalapril group(P＜0.05). Conclusions Telmisartan could not only control blood pressure steadily and effectively, but also decrease blood TG, increase HDL cholesterol and insulin sensitivity, and lower insulin resistance.

Objective To evaluate factors influencing long-term survival of biliary atresia patients receiving Kasai procedure and the prevention and treatment of surgical complications.Methods Among 97 biliary atresia patients undergoing Kasai or Suruga operation from Mar 1989 to Mar 1999, 35 cases have survived over five years.These 35 cases were classified intraoperatively into type Ⅱ (4 cases) and type Ⅲ (31 cases).Procedures included hepaticojejunostomy (12 cases), Suruga operation (hepaticojejunostomy with a jejunal fistula in 21 cases), hepaticocystomy (2 cases).Six patients underwent second hepaticojejunostomy because of porta hepatis obstruction.Results (1) Eighteen patients suffered from early cholangiolitis, and four cases developed late cholangiolitis, 5-8 years after closing the ascending stoma.(2) Five cases presented upper digestive tract hemorrhage caused by portal hypertension.(3) All patients were followed up from 5 to 15 years with 2 deaths.Conclusion Conventional surgical procedures still take important part in treating biliary atresia before liver transplantation is popularized.Factors influencing long term survival of patients receiving Kasai′s operation include age at operation, pathological classification of extrahepatic bile duct, with or without cholangitis after operation, and portal hypertension.

Objective To conduct comparative study on the analgesic and anti-inflammatory effects as well as the acute toxicity of yunaconitine and 8-deacetyl-yunaconitine isolated from the processed products of Aconiti Knsnezoffii Radix.Methods The methods of hot plate test and writhing test were used to evaluate the analgesic effect. Anti-inflammation action was observed by the models of auricle swelling caused by dimethylbenzene. LD50 was determined by the method of Bliss.Results Yunaconitine and 8-deacetyl-yunaconitine have analgesia effect on the pain caused by hot-plate, but there were no statistically significant difference. The pain caused by acetic acid had obvious analgesic action. High and low dose of yunaconitine could significantly reduce the number of mice body torsion and extend the incubation period of pain in mice. The effect of 8-deacetyl-yunaconitine was remarkable only in the high dose. Compared with solvent group, there were little differences in inhibiting effect of auricle swelling caused by dimethylbenzene, and anti-inflammatory action was not exact. The poisonousness of yunaconitine was nearly 20 times of 8-deacetyl-yunaconitine.Conclusion Yunaconitine and 8-deacetyl-yunaconitine may be the analgesic medicine for peripheral analgesic effect. The poisonousness of 8-deacetyl-yunaconitine is less than yunaconitine, the effect is remarkable to the pain caused by acetic acid, and the security is high.

Objective To evaluate the effectiveness of pulsative flushing and continuous flushing for peripheral venous catheter.Methods By retrieving Cochrane Library,ProQuest Research Library,PubMed,Web of science,Ovid,Embase and CINAHL complete(EESCO),China National Knowledge Infrastructure(CNKI),Wanfang,Weipu,and China Biology Medicine disc (CBMdisc) to find the randomized controlled trials (RCT) and clinical controlled trials(CCT) that been applied to compare pulsative flushing and continuous flushing for peripheral venous catheter,and using Revman5.3 soft for a Meta-analysis.Results There were thirteen studies which had involved 1 679 patients.The Meta-analysis' results as follows:The pulsative flushing was superior than continuous flushing indecreasing intravenous catheter's blockage (P ＜ 0.01),and had prolonged more time than continuous flushing in retaining catheter (P ＜ 0.01).The two flushing methods were similar in reducing phlebitis (P＞ 0.05).Conclusions The pulsative flushing was superior to the continuous one in reducing the intravenous catheter's blockage and prolonging the catheter's times.There were similar in decreasing phlebitis.The pulsative flushing method was used with caution in chronic diseases such as angiocardiopathy.