Objective To establish chronic obstructive pulmonary disease(COPD)rat models,and to study the intervention of the formula of nourishing Qi,activating blood circulation and dispersing phlegm recipe on the morphology.Method To establish rat COPD models by intratracheal instillation of lipopolysaccharide and exposure to cigarette smoke.To instill intervention drug daily either the formula of nourishing Qi,activating blood circulation and dispersing phlegm recipe or the roxithromythin,starting on the 20th,30th and 40th day of the experiment respectively(the groups was named h 20,h 30,h 40,r 20, r 30 and r 40 for short),and to observe the effect on the morphology by means of collagen staining and image analyzer.Results The pathological changes and lung function in the model group were accorded with the human COPD.In drug intervention groups,airway inflammation and epithelial proliferation were alleviated to different degree compared to the model group.In model group,the collagen deposition was increased predominant type I collagen compared to the health comtrol group,and the deposition in the drug intervention groups were decreased compared to the model group,according to the Sirius redpolarizing microscopy morphometry method.The thickness of the airway wall in the model group was significantly increased compared to the health control group(P

Objectives To study the psychological characteristics of children with attention deifcit hyperactivity disorder (ADHD). Methods Human ifgure drawings (HFD) as a projective technique was performed in 107 children with ADHD aged (8.69 ±1.48) years old (6.5~11.5 years), and 107 matched healthy children. Characteristic features of the HFDs in children with ADHD, and correlations between emotional indicators of the HFDs and factors of child behavior checklist (CBCL) were assessed and com-pared with the control group. Results Signiifcant differences in emotional index were found between ADHD and healthy chil-dren, including bad body composition, short arms, long arms, big ifgure, omission of hands and omission of feet (P<0.05). Nega-tive correlation was found between the HFDs emotional index and the factors of activity and social ability in the CBCL (P<0.05), but a positive correlation was found between the HFDs emotional index and behavioral problem (P<0.05). Conclusion Human ifgure drawings can relfect some emotional characteristics of children with ADHD, and when combined with CBCL, it can pro-vide evidence for diagnosis and intervention for ADHD.

Objective To study polymorphism of phosphatase and tensinhomologue (PTEN)-induced kinase 1(PINK1) gene of exon 5 in Parkinson patients who located in Guangxi ,and its relationship with Parkinson disease (PD) .Methods PCR ,single strand conformation polymorphism (SSCP) and sequencing analyze were conducted to analyze the PINK 1 gene′s exon 5 polymorphism in 28 cases of early-onset PD patients ,22 cases of late-onset PD patients(early-onset PD patients + late-onset PD patients = PD group) and 55 of control group .Results The intronic intervening sequence 5-5G-A (IVS5-5G-A ) polymorphism and G12164A polymer-phism which were located on PINK1 gene of exon 5 were chain relation .The G/A ,A /A genotype frequency was significantly higher in PD group (42 .0% ) than that in control group (23 .6% ) (χ2 = 4 .034 ,P= 0 .045) .The frequency was also significantly higher in late-onset PD patients (45 .5% )than that of 38 cases who were older than 50 years old in control group(21 .1% )(χ2 = 3 .951 ,P=0 .047) .There were no significant differences in alleles .Conclusion This research suggests that chain relation polymerphism at IVS5-5G-A and G12164A in PINK1 gene may be a susceptible factor for PD patients in Guangxi .

Objective To investigate the effect of acupoint massage on blood glucose in type 2 diabetic patients. Methods A total of 67 patients with type 2 diabetes mellitus patients, using the random number table method were divided into experimental group of 34 cases and 33 cases in the control group by random number table method. The experimental group was given five groups of acupoint massage and "five carriages" management to diabetes patients (control diet, exercise, blood sugar and lipid lowering drugs, diabetes knowledge propaganda and education, blood glucose monitoring). The control group was administered only the "five carriages" of diabetes. The two groups were observed for 2 months, and were compared the changes of biochemical indexes of glucose metabolism. Results After 2 months, the experimental group's fasting plasma glucose (FBG), 2 hours postprandial blood glucose (2 hBG), and glycated hemoglobin (HbA1c) decreased from the original (10.12±4.15) mmol/L, (15.32±5.65) mmol/L, and (9.36 ± 2.78)% to (6.04 ± 1.75) mmol/L, (8.42 ± 3.01) mmol/L, (6.35 ± 0.78) % , respectively, there were statistically significant differences within the group (t=5.28, 6.28, 6.08 respectively, all P<0.01). After 2 months, the FBG, 2 hBG, and HbA1c in the control group decreased from (11.23±6.02) mmol/L, (16.31± 6.23) mmol/L, and (9.67 ± 2.59) % to (7.12 ± 1.21) mmol/L, (9.58 ± 2.68) mmol/L, and (7.47 ± 1.96) % , respectively, and the differences within the group were statistically significant (t = 3.90, 5.79, and 3.95, respectively, all P<0.01). After 2 months, there was a significant difference between the FBG, 2 hBG, and HbA1c groups in the experimental group and the control group (t=2.96, 1.69, 3.09, P<0.05). There was no significant difference in the FBG and 2 hBG groups after 1 week of intervention in the experimental group (P>0.05). Conclusions To acupoint massage with basic intervention for type 2 diabetes patients can effectively consolidate the hypoglycemic effect, which is worthy of clinical reference and promotion.

Objective:To investigate the status of depressive symptoms of the elderly in Beijing community, and to explore its influencing factors.Methods:From October to December 2020, a cross-sectional survey was conducted among 224 senior citizens (aged 80 years old and over) who met the inclusion and exclusion criteria from 7 streets of Dongcheng District of Beijing by convenience sampling method. General demographic data sheet, the 6-item short form of University of California Los Angeles Loneliness Scale, Patient Health Questionnaire-9 (PHQ-9) , Quality of Life Questionnaire-6, Social Support Questionnaire and Activities of Daily Lives data sheet were used to collect information. Analysis of variance and statistical analysis were used χ 2 test was used to analyze the difference of depression degree among the elderly with different characteristics, and multiple linear regression was used to analyze the influencing factors of PHQ-9 score. Results:A total of 132 people were detected depression symptoms (PHQ-9≥5 points) , the detection rate was 58.9% (132/224) . Among them, 63 (28.1%) , 34 (15.2%) , 18 (8.0%) and 17 (7.6%) had mild, moderate, moderate and severe depression, respectively. Univariate analysis showed that the differences of depression degree of the elderly with different ages, marital status, living conditions, the occurrence of the hit events in recent two years, self-care ability, loneliness, quality of life and social support were statistically significant ( P<0.05) . Multiple linear regression analysis showed that age, loneliness, quality of life and social support were the influencing factors of the scores of PHQ-9 (ajusted R2=0.604, P<0.01) . Conclusions:The prevalence rate of depressive symptoms is high in the elderly aged 80 and above living in Beijing community. Age, loneliness, quality of life and social support are associated with depression of the elderly in the community.

Objective:To explore the effect of elastic band resistance training in the balance ability training of inpatients in Geriatric Department.Methods:From January to December 2019, 70 inpatients in the Geriatrics Department of a ClassⅡ general hospital in Beijing were selected and randomly divided into observation group and control group, with 35 cases in each group. Control group used routine exercise health education and supervised patients to complete the activity. On the basis of control group, observation group carried out balance training according to the elastic band resistance training method. Before and after the intervention, the differences in the grip strength, Short Physical Performance Battery (SPPB) , single-legged standing with eyes closed, and Timed up and Go (TUG) test of patients were compared between the two groups.Results:After 12 weeks of intervention, patients of observation group had grip strength (27.371±8.769) kg, total SPPB score (8.657±1.259) , time of single-legged standing with eyes close (5.151±1.551) s, and TUG test (14.031±3.666) s, higher than those of control group, the differences were statistically significant ( t=3.148, 5.505, 31.128, 2.269; P<0.05) . Conclusions:Elastic band resistance training can improve the limb strength and balance ability of elderly hospitalized patients, which can be further promoted.

BACKGROUNDIt has been known that vascular endothelial growth factor (VEGF) and its receptor (VEGFR2) play important roles in tumor angiogenesis. The aim of this study is to investigate whether an oral DNA vaccine against VEGFR2 has the inhibition effect on tumor growth and angiogenesis, and explore its mechanism in vivo.

METHODSC57BL/6 mice were respectively given the DNA vaccine encoding VEGFR2 (vaccine group), pcDNA3.1 (plasmid group) and saline (saline group). All the mice were then inoculated with Lewis lung carcinoma 3LL cells. Weight, size and microvessel density (MVD) of transplanted tumors were observed. The levels of CD3+ and CD8+ T cells in peripheral blood of mice were detected by flow cytometry.

RESULTSWeight of transplanted tumors in vaccine group was significantly smaller than those in plasmid and saline groups (P < 0.05), and MVD was significantly lower in vaccine group than that in plasmid and saline groups (P < 0.05). After inoculated with 3LL cells, CD3+ and CD8+ T cell levels of vaccine group were markedly higher than those of plasmid and saline groups (P < 0.05).

CONCLUSIONSThe oral DNA vaccine can significantly inhibit angiogenesis and growth of transplanted tumor in mice. It may act through killing endothelial cells of tumor.

Rift Valley fever (RVF) is an acute, febrile zoonotic disease that is caused by the RVF virus (RVFV). RVF is mainly prevalent on the Arabian Peninsula, the African continent, and several islands in the Indian Ocean near southeast Africa. RVFV has been classified by the World Organisation for Animal Health (OIE) as a category A pathogen. To avoid biological safety concerns associated with use of the pathogen in RVFV neutralization assays, the present study investigated and established an RVFV pseudovirus-based neutralization assay. This study used the human immunodeficiency virus (HIV) lentiviral packaging system and RVFV structural proteins to successfully construct RVFV pseudoviruses. Electron microscopy observation and western blotting indicated that the size, structure, and shape of the packaged pseudoviruses were notably similar to those of HIV lentiviral vectors. Infection inhibition assay results showed that an antibody against RVFV inhibited the infective ability of the RVFV pseudoviruses, and an antibody neutralization assay for RVFV detection was then established. This study has successfully established a neutralization assay based on RVFV pseudoviruses and demonstrated that this method can be used to effectively evaluate antibody neutralization.
Africa ; Animals ; Blotting, Western ; HIV ; Indian Ocean ; Islands ; Methods ; Microscopy, Electron ; Product Packaging ; Rift Valley fever virus ; Rift Valley Fever ; Zoonoses

Objective:To understand the current status of nurses' awareness and needs for the Employee Assistance Program (EAP), as to provide basis for the hospital to formulate EAP.Methods:Using the purpose sampling method, 352 ICU nurses from the Nanjing Drum Tower Hospital Affiliated of Nanjing University Medical School were selected as the research subjects from December 2019 to January 2020. Finally, 350 valid questionnaires were obtained. Use the EAP demand questionnaire to investigate the ICU nurses' awareness and demand for EAP.Results:Among the 350 nurses in the intensive unit, 214 cases (61.14%) had never understood EAP, only 8 (2.29%) had understood and experienced the EAP, and 332 cases (94.86%) believed that the hospital′s implementation of EAP was a benefit provided to nurses. The top two service requirements were: "Relief of work pressure" and "mental health consultation", and hope to receive mixed-mode EAP services; in ICU and CCU, nurses hope EAP can help resolve work-family conflicts; in EICU, they need to provide Specific job skills training services.Conclusions:ICU nurses have insufficient cognition of EAP and urgently need to obtain EAP implementation knowledge; at the same time, it is necessary to formulate an implementation plan that meets ICU nurses′ needs for EAP, that is, to relieve work pressure and carry out psychological counseling to ensure that EAP is launch successfully in ICU nurses.

Objective: To conduct the integrated management of hospital, community and family for patients with insulin injection at home, in order to explore the influence of this trinity health education model on the knowledge of medical waste and the standard disposal of discarded needles. Methods: The self-designed questionnaire was used to investigate the knowledge and disposal of medical waste in outpatients, and the causes were analyzed carefully after the problems were found. The hospital, community and family were timely communicated and fed back to the ward and community. After 1, 3 and 6 months of educational intervention, the disposal of insulin needles, the knowledge of medical waste and the recovery of sharp instrument boxes were observed. Results: After 1, 3 and 6 months of health education, the final rate of insulin needles mixed into domestic waste was 51.8% (144/278), 15.1% (42/278) and 4.7% (13/278), respectively. Compared with the first result of 99.6% (277/278), the difference was statistically significant (χ²=173.046, 406.136, 502.346, P<0.01); The first survey found that the correct rate of responding to medical waste knowledge items was low. After health education for 1, 3 and 6 months, the correct rate of medical waste related knowledge items was significantly higher than that of the first survey, and the difference was statistically significant (OR=3.016-3 548.810; 95%CI 2.108-917.869, 4.315-19 777.062; χ²=25.180-524.895; P<0.01) ; After 1, 3 and 6 months of health education, the qualified rate of medical waste knowledge was 82.4% (229/278),75.9% (211/278) and 97.1% (270/278), respectively, which was significantly higher than the first result of 6.5% (18/278). The difference was statistically significant (χ²=324.328, 273.712, 453.832, P<0.01). The sharp box recovery of hepatitis B patients was relatively good. Conclusion Hospital-community-family Trinity health education model can improve the breadth and depth of education objects, not only can improve the knowledge of medical waste related to insulin injection patients at home, but also can improve the standardized disposal ability of insulin waste needles. With the passage of time, the effect of health education continues to be good. This integrated health education model is worth popularizing in clinic.