BACKGROUND: Long term taking cyclosporin A(CsA)can inhibit osteoblastic differentiation and induce osteoporosis.Equol,which has greater binding affinity to estrogen receptors,can stimulate the proliferation and osteoblastic differentiation.OBJECTIVE: To investigate whether equol may protect against the proliferation and osteoblastic differentiation inhibited by CsA in mouse bone marrow mesenchymal stem cells(BMSCs)cultures and analyze signal pathway of protection.METHODS: Primary mouse BMSCs were cultured by using attachment method and assigned to five groups,which respectively treated with equol or/and CsA in the presence or absence of ICI182780,an estrogen receptor antagonist,and Nω-nitro-L-arginine methyl ester.Under an inverted microscope,morphological changes and mineralization ability of BMSCs were observed.The cell proliferation was measured by[3H]-thymidine incorporation.The osteoblastic differentiation and mineralization of extracellular matrix in BMSCs was assessed by measuring alkaline phosphatase activity and calcium deposition,respectively.Nitric oxide production in the conditioned media and cyclic guanosine monophosphata(cGMP)content in BMSCs were determined by using commercial nitric oxide and cGMP kit,respectively.RESULTS AND CONCLUSION: Equol reversed the decreased[3H]thymidine incorporation(P < 0.05),alkaline phosphatase activity(P < 0.05)and calcium deposition(P < 0.01)of CsA,which was accompanied with the changes of nitric oxide production(P < 0.01)and cGMP content(P < 0.01).The group by co-treatment with equol and CsA possessed higher cells growth density and small mineralized nodes than CsA group on day 12 under an inverted microscope.Moreover,the equol-reversed effect was abolished by ICI182780 and Nω-nitro-L-arginine methyl ester.These indicated that equol can reverse the inhibition of CsA on the proliferation and osteoblastic differentiation of mouse BMSCs through estrogen receptor/nitric oxide/cGMP signal pathway.

AIM:To establish the method of fingerprint analysis on Shuangbai Powder(Radix et Rhizoma rhei,Cacumen platycladi,Cortex phellodendri amurensis,Herba lycopi and Herba menthae)to distinguish the characteristic fingerprint.METHODS:HPLC with ZORBAX Eclipse XDB-C_(18) was used,acetonitrile-0.1% H_3PO_4 solution(gradient elution)as a mobile phase and detection wavelength at 254 nm,flow rate was 1 mL/min,and column temperature was 40℃.RESULTS:Thirty-seven common peaks were separated from 10 batches of Shuangbai Powder.The characteristic peaks were the summation,22 peaks were from Radix et Rhizoma Rhei,6 peaks were from Cortex phellodendri Amurensis,4 peaks were from Cacumen Platycladi,3 peaks were from Herba Lycopi,and 2 peaks were from Herba Menthae,there was one new characteristic peak.CONCLUSION:Ten peaks gathered from Shanghai Powder consist of rhein,emodin,chrysophanol,aloe-emodin,physcion,gallic acid,berberine hydrochloride,palmatine;quercitrosid,and linarin.

Objectives To study the psychological characteristics of children with attention deifcit hyperactivity disorder (ADHD). Methods Human ifgure drawings (HFD) as a projective technique was performed in 107 children with ADHD aged (8.69 ±1.48) years old (6.5~11.5 years), and 107 matched healthy children. Characteristic features of the HFDs in children with ADHD, and correlations between emotional indicators of the HFDs and factors of child behavior checklist (CBCL) were assessed and com-pared with the control group. Results Signiifcant differences in emotional index were found between ADHD and healthy chil-dren, including bad body composition, short arms, long arms, big ifgure, omission of hands and omission of feet (P<0.05). Nega-tive correlation was found between the HFDs emotional index and the factors of activity and social ability in the CBCL (P<0.05), but a positive correlation was found between the HFDs emotional index and behavioral problem (P<0.05). Conclusion Human ifgure drawings can relfect some emotional characteristics of children with ADHD, and when combined with CBCL, it can pro-vide evidence for diagnosis and intervention for ADHD.

Objective To establish a fingerprint analysis method for the medicinal slices of Radix et Rhizoma Rhei.Methods HPLC with ZORBAX Eclipse XDB-C18 was applied.The chromatographic conditions were as follows:acetonitrile-0.1 %H3PO4 solution(gradient alution) as a mobile phase and detection wavelength at 254 nm,flow rate at 1 mL?min-1,and column temperature being 40 ℃.Results Twenty-five common peaks were separated from 10 batches of medicinal slices of Radix et Rhizoma Rhei.Conclusion The method is reliable and accurate,and can be used as a quality control method for the medicinal material of Radix et Rhizoma Rhei.

Objective To establish a fingerprint analysis method for Ganzhixiao Decoction. Methods RP-HPLC was applied with Phenomenex Synergi 4?Hydro-RP 80A as the chromatic column,methanol-0.1 %H3PO4 solution (gradient elution) as the mobile phase,detection wavelength at 280 nm,flow rate being 1.0 mL/min,and column temperature at 30 ℃. Results Twenty common peaks were presented in 10 batches of Ganzhixiao Decoction and the positions of 20 characteristic peaks were identified,including 5-hydroxymethyl furfural,chlorogeni C acid,Salvianolic acid B. Conclusion The method is reliable and accurate,and can provide a reference for the research of Ganzhixiao Decoction.

AIM:To establish the method of fingerprint analysis on Shuangbai Powder(Radix et Rhizoma rhei,Cacumen platycladi,Cortex phellodendri amurensis,Herba lycopi and Herba menthae) to distinguish the characteristic fingerprint.METHODS:HPLC with ZORBAX Eclipse XDB-C18 was used,acetonitrile-0.1% H3PO4 solution(gradient elution) as a mobile phase and detection wavelength at 254 nm,flow rate was 1 mL/min,and column temperature was 40 ℃.RESULTS:Thirty-seven common peaks were separated from 10 batches of Shuangbai Powder.The characteristic peaks were the summation,22 peaks were from Radix et Rhizoma Rhei,6 peaks were from Cortex phellodendri Amurensis,4 peaks were from Cacumen Platycladi,3 peaks were from Herba Lycopi,and 2 peaks were from Herba Menthae,there was one new characteristic peak.CONCLUSION:Ten peaks gathered from Shangbai Powder consist of rhein,emodin,chrysophanol,aloe-emodin,physcion,gallic acid,berberine hydrochloride,palmatine;quercitrosid,and linarin.

AIM: To establish a method of fingerprint analysis on Cacumen Platycladi. METHODS: HPLC was adopted with ZORBAX Eclipse XDB-C18,acetonitrile-0. 1% H3PO4 solution (gradient alution) as a mobile phase and detection wavelength at 254 nm,flow rate was 1 mL/min,and colum temperature was at 40 ℃. RESULTS: Eleven common peaks were separated from 14 batches of Cacumen Platycladi. As compared with standard sample,quecitroside and quecitin were checked out. CONCLUSION: The method is reliable,accurate and can be used as a quality control method for Cacumen Platycladi.

Objective To study the clinicopathologic features and prognosis factor of Chinese lymphoblastic lymphoma.Methods 105 LBL cases were collected.Routine HE staining,immunostaining were used to investigate the clinicopathologic features,immunotype.Results The ratio of male to female was 1.76:1 (67:38),the medial age was 13 years old (0-73 years old).53 cases (53/105,50.48 ％) primarily showed lymph node involvement,including 34 cases (34/53,64.15 ％) showed jugular node involvement;mediastinum (12/52,23.08 ％) was the most frequent extranodal involvement site.69 cases (69/105,65.71％)showed bone marrow involvement with 15 cases as the primary involvement.The expression of TdT,CD99,CD3(E),CD20,CD79a,PAX5,MPO,CD34 and CD117 was 84.76 ％ (89/105),85.00 ％ (68/80),54.37 ％ (56/103),16.67 ％ (16/96),40.00 ％ (8/20),46.67 ％ (28/60),14.29 ％ (8/56),25.00 ％ (4/16) and 0.All cases were divided in to two groups,62 cases as T-LBL (59.05 ％,62/105) and 33 cases as B-LBL (31.43 ％,33/105),with 9 cases (8.57 ％,9/105) without the expression of T or B cell marker and 1 cases with the expression of both T and B cell marker.61 cases were detected the expression of myeloid markers and 8 cases were positive.All cases were followed up.The medial survival was 36 months.The survival at 1 year,2 year and 3 year was 66.67 ％ (70/105),48.81％ (41/84) and 20.69 ％ (12/58) respectively.Age was the prognosis associated factor.The children had better progonosis than adults.Immunotype,bone marrow involovement and transplantation didn' t show prognosis indicator (P ＞ 0.05).Conclusion Most of Chinese LBL occurs in child and younger male,multi-lymphadehypertrophy and bone marrow involvement are common.LBL is an aggressive tumor.Age is the prognosis associated factor.Children have a better prognosis than the adult.

Objective To study the process conditions of purifying the total flavonoids from Cacumen Platycladi by AB-8 resin. Methods Spectrophotometric method was used to detect the total flavonoids,and HPLC was used to determine quercitrin and analyze the characteristic peaks of Fingerprint. Results The purification effect was satisfactory when the concentration of original solution of the extract of Cacumen Platycladi was 0.20 g?mL-1,the loading amount was 0.375 g of Cacumen Platycladi per 1 mL of wet AB-8 resin,the adsorption velocity was 1 mL?min-1,the eluant was 70 %alcohol being 4 times as much as the resin volume,and the elution velocity was 2 mL?min-1. AB-8 resin could be used for 3 times repeatedly after being reproduced by 95 %of ethanol and 1 moL?L-1 of natrium hydroxydatum (NaOH). The remaining rate of the total flavonoids and quercitrin was over 70 %and 95 %respectively,and the remaining rate of peaks 1～3 was over 90 %. After being purified by the B-8 resin,the contents of the total flavonoids and quercitrin were raised 2.48 times and 3.29 times more than those before purification respectively. Conclusion AB-8 resin is fit for separating and purifying the total flavonoids from Cacumen Platycladi.

Objective To explore the strategies and effectiveness of perioperative fluid administration during pediatric kidney transplantation.Methods In our study,we evaluated the heart function and compliance of pediatric patients preoperatively,and then formulated perioperative fluid management strategy according to children’s circulation feature.The electrolytic balance was kept and complications were prevented.Results Twenty-nine out of all 48 children experienced electrolyte disturbance to different extents on day 1 postoperatively,which improved on day 2 after prompt management.There were five cases of oliguria,three cases of delayed graft function(DGF),one case of heart failure and multiple organ failure respectively.Conclusion Such nursing strategies like formulation of reasonable fluid infusion plan according to the physiological features of pediatric recipients,keeping the electrolytic balance and careful management of fluid at polyuria,oliguria and anuria,care critical for the children to live through the perioperative periods.