1.A reliable method to induce neural stem cells directed differentiating into neurons in vitro
Acta Anatomica Sinica 2009;40(4):675-679
Objective To introduce a reliable method to induce neural stem cells directed differentiating into neurons in vitro. Methods The rat neural stem cells were cultured in selected serum-free medium. After cultured for 2, 3 passages,the neurospheres or single-cells isolated from neurospheres were cultured in conditioned medium to induce directed differentiating into neurons. Besides morphological observation of those cells under inverted microscope, NSE immunocytochemistry was carried out to detect the differentiating ratio of those cells from neural stem cells into neurons. Results It was shown that the conditioned medium could induce neural stem cells differentiating into neurons effectively in both neurospheres culture mode or single-cells culture mode. Furthermore, compared with the neurospheres culture mode, the single-cells culture mode showed more synchronous in the differentiation process and higher in the percentage of NSE-positive cells. Conclusion This method can induce neural stem cells directed differentiating into neurons effectively and stablely.
2.Mediating effects of self feeling of inadequacy between resilience and alexithymia in depressive undergraduates
Fenglian FENG ; Ming LI ; Ruofan LI ; Bingzhang LI ; Cuili CAO
Chinese Journal of Behavioral Medicine and Brain Science 2016;25(1):70-75
Objective To study the relationship among resilience,self feeling of inadequacy and alexithymia in depressive undergraduates,and to provide a theoretical basis for the prevention and intervention of depression among undergraduates.Methods 500 undergraduates of two universities in Hebei Province were collected by cluster sampling method.Beck depression scale,adolescent resilience scale,Toronto alexithymia scale and self feeling of inadequacy scale were applied to undergraduates.The survey results were analyzed statistically.Results The detection rate of depression was 28%(119/426) in undergraduates.Total score of Alexithymia Scale(69.99±9.43) was higher than the norm(65.70±7.98),and the difference was statistical significant (P<0.05).The score of self feeling of inadequacy had a significant difference between male (149.88±28.00) and female(138.58±28.79) (P<0.01).Neither gender nor grade had significant difference in depression,alexithymia and resilience(P>0.05).Self feeling of inadequacy was positively correlated with alexithymia (P<0.05).Resilience was negatively correlated with self feeling of inadequacy and alexithymia(P<0.05).For the depressive undergraduates,self feeling of inadequacy served as a full mediator between the resilience and alexithymia,whereas for the non depression undergraduates it was a partial mediator.Conclusion Depression undergraduates have serious alexithymia,serious self feeling of inadequacy and poor resilience.Self feeling of inadequacy serves as a mediator between the resilience and alexithymia in depressive and non-depressive undergraduates.
3.Effect of adenovirus-mediated IL-24 gene on the topoisomeraseⅡα and Caspase-3 expression in glioma cell line U251
Shuo HAN ; Huixian CUI ; Wenling LI ; Junxia ZHAO ; Cuili CAO ; Yunli YAN
Acta Anatomica Sinica 2010;41(1):48-52
Objective The present study is to investigate IL-24 gene(Ad5F35-hIL-24) effect on the topoisommeraseⅡα(topoⅡα) and Caspase-3 expression in glioma cell line U251. Methods After transfected the U251 glioma cells with the Ad5F35-hIL-24, the methyl thiazolyl tetrazolium (MTT) was used to analyse the inhibition rate of Ad5F35-hIL-24 on the cells. Hoechst 33258 fluorescent staining and flow cytometric assay were used to detect apoptosis. The immunohistochemistry assay was used to detect topoⅡα expression, and Western blotting was applied to detect the protein expression of topoⅡα and caspase-3. Transwell experiment was used to test the invasiveness of the cells. Results It was found that the Ad5F35-hIL-24 could inhibit U251 cell proliferation and induce apoptosis in a dose dependent manner compared with the control groups. It showed that Ad5F35-hIL-24 could inhibit topoⅡα expression reveled by immunohistochemistry and Westeren blotting, while it increased caspase-3 protein expression. The Transwell experiment showed that the Ad5F35-hIL-24 could reduce the invasiveness of the U251 glioma cells.Conclusion The exogenous IL-24 gene can inhibit the cell proliferation and induce apoptosis of U251 glioma cells. The topoⅡα and Caspase-3 are the important molecular targets of the IL-24 gene. These results may give support for the IL-24 gene usage in clinical treatment for glioma patients.
4.Preventive Effect of Caffeine on Alzheimer's Disease
Qianqian HOU ; Xuejiao CAO ; Jiabao WANG ; Wenjing GUO ; Xudong HOU ; Cuili ZHANG
Progress in Modern Biomedicine 2017;17(23):4452-4455,4571
Objective:To explore the effects of caffeine on the prevention of Alzheimer's disease (AD).Methods:Use Ethanol as a solvent to extract the caffeine in tea and then injecting 5% D-galactose saline solution 1ml/d/kg to establish aging model mice.Divide mice randomly into experimental group (high-dose/low-dosecaffeine),positive control group,negative control group,and normal con-trol group (NS) and injecting appropriate drugs for consecutive four weeks.Test superoxyde dismutase (SOD) and malondialdehvde (MDA) periodically.Take mice's hippocampus and use Western blotting to detect the expression of brain derived neurotrophic factor (BDNF) and extracellular signal-regulated kinasesl/2 (p-ERK1/2).Results:The expression of BDNF and p-ERK1/2,negative control group is less than low-dose experimental group and positive control group (P<0.01);The p-ERK1/2 expression of injecting D-galactose mice was significantly lower than normal group,negative control group compared weth the normal group,the differencd was significant (P<0.05).The level of SOD in model group was significantly lower than that in normal control group,high,low dose caffeine group and positive control group (P<0.01),but the level of MDA is opposite.Conclusions:Caffeine can delay aging process by increasing the level of SOD in aging mice,and enhancing the expression of BDNF and P-ERK1/2.Caffeine does a lot to prevent AD.
5.The effect of moderate running wheel exercise on the learning and memory ability and hippocampal neurogenesis in young mice
Yang SHI ; Bingzhang LI ; Cuili CAO
Chinese Journal of Behavioral Medicine and Brain Science 2019;28(4):322-325
Objective To observe the effect of moderate running wheel exercise on the learning and memory ability and hippocampal neurogenesis in young mice.Methods Twenty male 1 month old Kunming mice were randomly divided into control group and exercise group.After 8 weeks of running wheel exercise in the exercise group,the Morris water maze test was used to detect the spatial learning and memory ability of the two groups of mice.Immunohistochemical technique was used to detect the expression of Sox2,Ki67 and DCX in the dentate gyrus of mice in two groups,and those specific protein can reflected the hippocampal neurogenesis.Results In the place navigation test of Morris water maze,the latency of the exercise group ((29.00± 1.32) s) was lower than that of the control group ((36.30±0.69) s),and the difference was statistically significant (t=5.154,P<0.05).In the spatial probe test,the number of times of crossing platforms in the exercise group ((3.73±1.51) times) was more than that of the control group ((1.89±t 1.63) times),and the difference was significant (t=3.583,P<0.05).Immunohistochemical results showed that the number of Sox2,Ki67 and DCX immunoreactive cells in the dentate gyrus region of the exercise group were ((284.40± 31.50),(54.50± 10.75),(77.80=t± 11.60) respectively) more than those in the control group ((241.40± 10.57),(37.00± 7.81),(48.20±t 11.86) respectively),and the difference was statistically significant (t =4.129,5.789,7.971,all P<0.01).Conclusion Moderate running wheel exercise can significantly improve the learning and memory ability of young mice,which may be related to the promotion of neurogenesis in the dentate gyrus of the hippocampus.