1. Segmental absorption of helicid in rat everted intestinal sac model: A preliminary study
Ping WANG ; Nan GUO ; Haitang XIE ; Cuijiao ZHAN ; Changmao WANG ; Yuanwei JIA ; Jie SHEN ; Bin YANG ; Yuanwei JIA ; Jiru CHU ; Dandan ZHENG ; Rongfeng HU
Chinese Journal of Clinical Pharmacology and Therapeutics 2021;26(3):258-263
AIM: To investigate the absorption of helicid in different segments of intestine based on rat everted intestine sac model. METHODS: To establish a high-performance liquid chromatography method for simultaneous determination of helicid and its metabolite. Krebs-ringer solution containing helicid was added to everted intestine sacs of different segments (duodenum, Jejunum, ileum and colon). Drug concentration in sacs was determined at different time points (5, 10, 15, 30, 45, 60, 75, 90 min). Adsorptions of helicid in four intestinal segments were compared. RESULTS: This high-performance liquid chromatography was successfully applied to the simultaneous determination of helicid and its metabolite. Absorption of helicid was rapid and time-dependent. The absorption and metabolism of helicid in duodenum segment were higher than these in other segments. CONCLUSION: The duodenum segment is the main site of segmental absorption and metabolism of helicid. This is the first report on intestinal segment metabolism of helicid.
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