1.¹H NMR metabonomics study of pancreatic extracts from insulin-resistant rats induced by fructose feeding.
Linlin WANG ; Lingyun ZHENG ; Lei ZHANG ; Ali CHEN ; Cuihuan QIU ; Jingfen XU ; Yongxia YANG
Journal of Southern Medical University 2014;34(9):1301-1304
OBJECTIVETo study the metabolic changes of pancreatic extracts from insulin-resistant rats induced by fructose feeding using nuclear magnetic resonance ¹H spectroscopy (¹H NMR).
METHODSSixteen Wistar rats were divided equally into control group and model group and given water and 10% fructose water for 8 weeks, respectively. The pancreatic tissues were then obtained for H NMR spectra analysis and principal component analysis (PCA).
RESULTSCompared with the control rats, the rats in the model group showed significantly increased creatine, betaine/TMAO, taurine, glycine and myo-inositol and decreased levels of lipids, lactate, glutamate, choline and GPC/PC.
CONCLUSION¹H NMR and pattern recognition can define the metabolic characteristics of the pancreatic tissue extracts from insulin-resistant rats and provide reliable metabolic evidence for studying the mechanisms of insulin resistance at the molecular level.
Animals ; Fructose ; Insulin Resistance ; Metabolomics ; Pancreatic Extracts ; chemistry ; Principal Component Analysis ; Proton Magnetic Resonance Spectroscopy ; Rats ; Rats, Wistar
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