Objective To observe the effect of glycyrrhizin (CG) on the proliferation and the expressions of interleukin (IL)-2, IL-4 in human keratinocytes cell line HaCaT cells, and to investigate the therapeutic mechanism of CG in the pa?tients with psoriasis. Methods The HaCaT cells were divided into four groups:blank group (without CG), low concentration group (50μL CG/mL cell supernatant), medium concentration group (100μL CG/mL cell supernatant) and high concentra?tion group (200μL CG/mL cell supernatant). Methyl thiazolyl tetrazolium (MTT) assay was performed to evaluate the prolifer?ation of HaCaT cells that were treated with CG at different concentrations respectively for 24 hours. The contents of IL-2 and IL-4 in HaCaT cells were examined by enzyme linked immunosorbent assay (ELISA). Results Compared with the blank group, there were obviously inhibitory effects in the proliferation of HaCaT cells treated by low,medium and high concentra?tions of CG for 24 hours. There was no significant difference in IL-2 level between low concentration CG group and blank group (ng/L:234.51±17.98 vs 225.31±16.23, P>0.05). After 24 hours of intervention with CG of medium and high concen?trations, the expression levels of IL-2 was significantly lower in HaCaT cells than that in blank group and low concentration group. The IL-2 level was significantly lower in high concentration group than that in medium concentration group (ng/L:188.99±19.22 vs 208.49±18.40, P<0.05). At the same condition, the secretion of IL-4 in HaCaT cells was significantly up-regulated in high concentration group than that in other three groups (ng/L:45.67±10.29 vs 37.62±3.90, 39.68±6.08, 43.85± 10.26, P<0.05). Conclusion Glycyrrhizin may suppress the proliferation of human skin keratinocytes by regulating secre?tion of cytokines IL-2 and IL-4.

After surgical removal of a primary tumor the minimal residual cancer cells (MRCC) and metastases derived thereof are the actual targets for all theraputic approaches. Due to the great sensitivity and PCR-based detection systems, the molecular characterization of MRCC can provide information about their metastatic potential, availability of drug targets, drug sensitivity and development of therapy resistance, which will close the analytical gap between primary disease and the detection of metastases by conventional methods such as imaging procedures, and this will help therapy selection, monitoring of the treatment effects and predicting of the prognosis. Patients will benefit from a individualized therapy in the end.

Objective To investigate the effect of optimizing rescue time for patients with acute st-elevation myocardial infarction (STEMI) in the hospital. Methods A retrospective analysis of the clinical data of 133 patients with ST-elevation myocardial infarction who were hospitalized in the first affiliated hospital of university of science and technology of china during July,2016 to June,2017 was performed. Timeline in the rescue, the result of coronary reperfusion and satisfaction degree of patients were analyzed. Results The rapid evaluation time (F=2.609, P=0.046),emergency handling time(F=7.581, P=0.032), login and logout time (F=5.667, P=0.017)and visit-ballon time (F=8.942, P=0.007) were shortened quarter by quarter . The average time of each project in the four quarters showed a statistically significant difference. The difference of TIMI classification of coronary flow reperfusion among the four quarters was statistically significant (H=8.402, P=0.038). The satisfaction degree of each quarter showed a statistically significant difference (the third quarter of 2016:94.68±2.38, the fourth quarter of 2016:96.72± 5.10, the first quarter of 2017:97.23 ± 7.64,the second quarter of 2017:98.36 ± 4.86;F=7.891,P=0.048). Conclusions Enhancing timeliness of emergency care can remarkably shorten rescue time, improve satisfaction degree of patients and help to improve the success rate of emergency treatment for patients with STEMI.