Phytoestrogens (PEs) and estrogen receptorβ(ERβ) have close relationship with breast cancer. PEs preferentially binds to ERβ rather than ERa. Although there are still some arguments, it provides a new though for researching the mechanism of Phytoestrogen-containing herbs on breast cancer and exploring the essence of TCM theory.

Objective To evaluate the immunogenicity of a recombinant adenovirus containing the porin IB (PIB) gene and Neisseria surface protein A (NspA) gene of N.gonorrhoeae in BALB/c mice.Methods A recombinant adenovirus containing the PIB gene and NspA gene of N.gonorrhoeae (pAdEasy-1-PN) was constructed in previous studies.Thirty BALB/c mice were randomly and equally divided into 5 groups:low-,medium-and high-dose experiment group intramuscularly immunized with 104,106,and 108 50％ tissue culture infective dose (TCID50) of the recombinant adenovirus pAdEasy-1-PN,respectively,pAdEasy-1 control group immunized with 106 TCID50 of pAdEasy-1,blank control group immunized with sodium chloride physiological solution.Immunization was carried out twice at a 4-week interval.Serum samples were collected at 0,3 and 5 weeks after the first immunization,and spleens were removed at 5 weeks followed by the isolation of spleen lymphocytes.Enzyme linked immunosorbent assay (ELISA) was performed to determine the serum levels of PIB-specific and NspA-specific antibodies,methyl thiazolyl tetrazolium (MTT) assay to evaluate the proliferaton activity of spleen lymphocytes after stimulation by the recombinant PIB and NspA antigens.A slide agglutination test was used to estimate the anti-bacterial activity of murine serum.The complement-dependent bacteriolytic activity of murine serum was also evaluated.Statistical analysis was carried out by t test.Results Both humoral and cellular immune response specific to PIB and NspA were elicited by the recombinant adenovirus in mice.At 3 and 5 weeks after the immunization,significant differences were observed in the serum levels of PIB-specific (F =285.72,564.83,respectively,both P ＜ 0.01) and NspA-specific (F =521.57,542.61,respectively,both P ＜ 0.01)antibodies.Also,the proliferation index was statistically different among these groups in spleen lymphocytes stimulated with PIB and NspA (F =171.61,233.96,respectively,both P ＜ 0.01).The vaccination efficiency was positively correlated with the inoculation dose of recombinant pAdEasy-1-PN,and 108 TCID50 per dose proved to be the optimal dose for immunization.The sera from mice immunized with pAdEasy-1-PN could agglutinate N.gonorrhoeae and kill it in the presence of complement.Conclusions The recombinant adenovirus pAdEasy-1-PN containing PIB and NspA genes could induce specific humoral and cellular immune response in mice,and may be a potential vaccine against N.gonorrhoeae.

Objective To study genital toxicity and didymus lipid peroxidation level on rats after sub-acute exposure to di-2-ethylhexyl phthalate(DEHP).Methods The weaning Wistar andro-rats were dividied into four group randomly,and there were 10 rats in each group.Compared with the corn oil control,the experiment groups were gavaged consecutively for 30 days with the DEHP dose of 10,100,1 000 mg/(kg.d),The change of body weight was observed dynamically.All rats were executed by decapitation four weeks later and the didymium was removed immediately,at the same time viscera coefficient were measured.The level of MDA were detected by TBA method,meanwhile GSH level and GSH-Px activity were analyzed by modified DTNB method,the activity of SOD were measured by xanthine oxidase method.Results With the increasement of DEHP concentration,the body weight growth of rats in 1 000 mg/(kg.d) DEHP group is restricted compared with the control(P

Objective To research the protective effects of alkaline electrolytic water on lipid peroxidation in blood,liver,kidney and brain of mice. Methods Total forty-five Kunming mice were randomly divided into three groups,control group:tap water;peroxidation model group:100 mg/kg D-galactose subcutaneous injection (once a day) + tap water;experimental group:100 mg/kg D-galactose subcutaneous injection (once a day) + alkaline electrolytic water. After 18 weeks of exposure,the mice were sacrificed,and the blood,liver,kidney and brain of mice were collected for further study. The MDA levels were measured by TBA method; The GSH levels and GSH-Px activities were measured by modified DNTB method; The SOD activities were measured by Xanthine oxidase method; The lipofuscin levels were detrmined by Sohal method. Results Compared with the control group,MDA and lipofuscin level increased,GSH content decreased,the activities of GSH-Px and SOD decreased in all detected organs and blood in the peroxidation model group,and compared with the peroxidation model group,MDA and lipofuscin level decreased,GSH content increased,the activities of GSH-Px and SOD increased significantly in D-galactose +alkaline electrolytic water group. Conclusion Alkaline electrolytic water has some antagonistic effects on the lipid peroxidation induced by D-galactose.

Objective: To establish a microbial limit test method for compound benzocaine gel.Methods: According to the general principles of Chinese Pharmacopoeia (2015 edition), method applicable experiments were performed respectively for the routine method, neutralization method and dilution & neutralization method.Using the recovery ratio of test bacteria as the index, the medium of dilution & neutralization method was adopted in the total aerobic microbial count and total combined yeasts and molds count, and the neutralization method was used for the control bacteria detection.The neutralizing agents were polysorbate 80 and lecithin.Results: The method of dilution & neutralization could eliminate the bacteriostasis of the drug, and the recovery ratio of each test bacteria was within the range of 0.5-2.0, which was in line with the requirements of Chinese Pharmacopoeia.Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus could be detected out in the positive control bacterial test,and bacteria were not detected out in the negative test.Conclusion: The method can be used for the microbial limit test for compound benzocaine gel.

We studied the genetic characteristics of Spermophilus in Shaanxi Province,China.The COI,Cyt-b gene were sequenced and the results were compared with those of dauricus from Inner Mongolia Keyouzhong Banner and Zhengxiangbai Banner,and S.alaschanicus from Haiyuan County of Ningxia.And genetic distance was analyzed and Neighbor-Joining tree was built.Results showed that the genetic distance of COI gene sequences between Spermophilus from Dingbian County in Shaanxi and S.alaschanicus in Ningxia was ≤0.5％,and the genetic distance was ranged from 7.9％ to 9.3％ with Citellus dauricus from Inner Mongolia.The genetic distance of Cyt-b gene between Spermophilus from Dingbian County in Shaanxi and S.alaschanicus in Ningxia was ≤2.2％,and ranged from 8.9％ to 11.2％ with Citellus dauricus from Inner Mongolia.The Neighbor-Joining tree of COI,Cyt-b gene showed two major clusters.One of them were clustered by Spermophilus from Dingbian County in Shaanxi and S.alaschanicus in Ningxia,and another one was Citellus dauricus from Inner Mongolia.The Neighbor-Joining tree of COI gene showed that all samples from Shaanxi Province clustered in a group.In conclusion,the Spermophilus in Shaanxi Province were S.alaschanicus.

Objective Observing the effect of Shuganyishen formula on hormone-dependant metastatic breast cancer and the mechanisms of anti-resistance and endocrine therapy. Methods 226 ITT cases were divided into two cohorts by treatment. 126 cases were treated with the combination of Shuganyishen formula and standard western medicine including endocrine therapy, and 100 cases were treated with western medicine alone. The end points were time to progress (TTP) and overall survival and quality of life (QOF). The effect of Shuganyishen formula on growth of TAM-resistance cell line in vitro was observed. Results There were statistical significances of median TTP between combination and western medicine in Luminal A (ER+ /PR+, HER2-) as well as Luminal B (ER+/PR+, HER2+), but no significances of OS; The extent and incidence of hot flush, sweat and syndromes related to bone were decreased significantly in combination treated group. Shuganyishen formula could reverse resistance to TAM. Conclusion Patients with LuminalA and LuminalB metastatic breast cancer benefit from Shuganyishen formula, which maybe related with its reverse resistance to endocrine therapy.

Objective:To evaluate the diagnostic value of HPV detection in squamous cell carcinoma of the cervical lymph node metastasis from an unknown primary site.Methods:Clinical data of 6 patients who were initially diagnosed with squamous cell carcinoma of the cervical lymph node metastasis from an unknown primary site and eventually diagnosed with HPV-related oropharyngeal squamous cell carcinoma were collected, and the process of diagnosis was analyzed.Results:Upon the initial admission, all patients were diagnosed with squamous cell carcinoma of the cervical lymph node metastasis with positive p16 expression, positive HPV-16 subtype and negative EBER expression. No obvious primary lesion was found after comprehensive examination. Subsequently, four of them underwent ipsilateral tonsollar blind biopsy ( n=2) and ipsilateral tonsillectomy ( n=2). All these four patients were pathologically diagnosed with tonsillar squamous cell carcinoma. For the other two cases, MRI detected the thickening complicated with enhancement of ipsilateral wall of oropharynx and tongue root after follow-up for D149 and D545 , respectively. Biopsy confirmed the diagnosis of squamous cell carcinoma of the tonsil and tongue root, respectively. Conclusion:For patients with HPV-positive squamous cell carcinoma of the cervical lymph node metastasis from an unknown primary site, the possibility that the primary lesion originates from the oropharyngeal site, especially the tonsil and tongue root, should be highly suspected.

Objective:To explore the application of 4C teaching combined with case simulation teaching in the practice teaching of respiratory and critical care medicine nursing students.Methods:Eighty nursing practice students from 2019 to 2021 in the Department of Respiratory and Critical Care Medicine of Beijing Chaoyang Hospital Affiliated to Capital Medical University were selected as the research objects, and they were randomized into control group ( n=40) and observation group ( n=40). The control group adopted traditional teaching, and the observation group adopted 4C teaching combined with case simulation teaching. The nursing comprehensive ability and self-directed learning ability of the two groups of nursing students were compared, and the evaluation of teaching methods between the two groups was assessed. SPSS 22.0 was used for Chi-square test and t-test. Results:The scores of theoretical knowledge, ward management, prevention and control of hospital infection, basic nursing, standardized writing of nursing documents, and critical care of nursing students in the observation group were higher than those in the control group ( P<0.05); the scores and total scores of learning awareness, learning behavior, learning strategies, interpersonal relationships, and learning evaluation in the observation group were higher than those in the control group ( P<0.05). The observation group believed that their teaching method was better than the control group in terms of mobilizing learning initiative, improving basic nursing practices, promoting clinical communication skills, improving the ability to analyze and solve problems, enhancing the concept of humanistic care, helping to understand and master knowledge, and improving the overall quality ( P<0.05). Conclusion:The 4C teaching combined with case simulation teaching can improve the comprehensive nursing ability and self-directed learning ability of nursing students in department of respiratory and critical care medicine, and the nursing students have a high opinion of the teaching method.