OBJECTIVE To explore and evaluate the efficacy of preventing the central venous catheter-related infection using catheter-sealing separately with gentamicin and heparin.METHODS One hundred and thirty six hemodialysis patients with temporary indwelling central venous catheters were enrolled in this study,and randomly assigned into 3 groups: Group A(catheter-sealing separately with gentamicin and heparin,n=46),Group B(catheter-sealing with gentamicin mixted heparin,n=45) and Group C(catheter-sealing with heparin,n=45).Complications such as infection were monitored.RESULTS Rate of catheter-related infection and intravenous catheter infection were without significant difference between Groups A and B,but were obviously lower than that in Group C(6.5% and 4.4% vs 22.2%,P