Objective To observe the clinical effects of trabeculectomy and implantation of amnion on closed-angle glaucoma. Methods 48 cases(55 eyes) were treated with trabeculectomy. Implant the anmion in scleral valva intraoperative, the sight,intraocular, procurvation and filtering bubble postoperative between 6 to 18 months were observed. Results The preoperative intraocular was (32. 43±5.76) mm Hg and the postoperative intraocular was ( 16. 63±4. 07) mm Hg. there were significant differences between the two intraoculars. The rate of functional filtering bubble formation was 89% with no rejection and no serious complication. Conclusion Trabeculectomy and implantation of amnion could reduce the formation of filtering bubble cicatrices. It also could improve the operations success rate.

Objective To evaluate the effect of refractive correction on the dynamic variation of diopter in children with hyperopia ametropic amblyopia. Methods Optometry with 1 % atropine oculentum was used to examine 105 patients(210 eyes) with ametropic amblyopia aged 3～6 years old. The diopter of all the cases was compound hyperopic astigmatism. The prescriptions of spectacles were made according to that the diopter of retinoscopy subtracted different degree hyperopia. Thus,all the patients were divided into group 1(subtract 20% off the diopter of retinoscopy,30 cases),group 2(subtract 25% off the diopter of retinoscopy,40 cases),group 3(subtract 30% off the diopter of retinoscopy,35 cases). During three years' treating periods, their dynamic variation of hyperopia diopter were observed time yearly. Results The hyperopia diopter of each group reduced year after year. But the average reduction of hyperopia diopter of group was significantly lower than that of other two groups(P0.05).Conclusion Refractive corrections of different degree have effect on emmetropization of children with hyperopia ametropic amblyopia. Moderate undercorrection is useful on emmetropization of children with hyperopic ametropic amblyopia.

AIM: To investigate the genes differential expression in cortex during rat focal cerebral ischemia. METHODS: cDNA microarray chips containing numerous cDNAs were used to investigate the gene expression pattern between samples of focal cerebral ischemia and sham-control operation rats. RESULTS: Two hundred and eleven genes differentially expressed were screened out, among these genes, up-and down-regulated genes were 199 and 12, respectively. CONCLUSIONS: The analysis of gene expression pattern of focal cerebral ischemia based on cDNA microarray can realize high-throughput screening of the genes associated with the focal cerebral ischemia. The differential expression of genes may be related to the pathogenesis of focal cerebral ischemic diseases.

Objective To investigate the effects of enteral immunonutrition supplemented with omega-3 polyunsaturated fatty acid (ω-3 PUFA) on inflammatory response,intestinal mucosal barrier function and the prognosis in patients with severe traumatic brain injury (sTBI).Methods 122 patients of sTBI hospitalized between January 2015 and December 2016 were randomly divided into experimental group (ω-3 PUFA,n=61) and control group (n =61).The serum levels of tumor necrosis factor-α (TNF-α),interleukin (IL)-6 and neuron specific enolase (NSE) were tested with enzyme linked immunosorbent assay.Meanwhile,D-lactate acid and intestinal fat acid binding protein (I-FABP) were evaluated by enzymology spectrophotometer method.After 14 days of treatment,the Glasgow Coma Scale (GCS) scores,Acute Physiology and Chronic Health Evaluation (APACHE) Ⅱ scores and prognoses of both groups were compared.Results The serum levels of inflammatory factors (TNF-α and IL-6),intestinal mucosal barrier function indicators (D-lactate acid and I-FABP) and NSE proteins significantly increased after sTBI (P =0.01).Compared with the control group,the experimental group on day 3 had significantly lower serum levels of inflammatory factors [TNF-α:(107.77± 19.79) μg/Lvs.(151.76±21.65) μg/L,P=0.01;IL-6:(76.85±7.15) μg/Lvs.(105.27±10.12) μg/L,P=0.01] and intestinal mucosal barrier function indicators [D-lactate:(69.81 ±6.32) μg/L vs.(89.80± 8.75) μg/L,P=0.03;I-FABP:(40.81±6.73) μg/Lvs.(56.60±8.58) μg/L,P=0.01].On day 7,the experimental group had significantly lower expression of NSE proteins than the control group [(13.63± 2.53) μg/L vs.(19.12±3.00) μg/L,P=0.02].The experimental group received better prognosis compared to the control group on day 14 [GCS scores:(9.74±0.76) vs.(8.44±0.53),P=0.04;APACHE Ⅱ scores:(14.67±1.37) vs.(17.53±1.47),P=0.03].The experimental group also had fewer days in hospitalization [(19.37±2.27) d vs.(25.42±2.61) d,P=0.01].Conclusion Enteral immunonutrition supplemented with ω-3 PUFA can effectively regulate the inflammatory response,and reduce impairment to the intestinal mucosal barrier function and damage to neurons in patients with sTBI.

The standard of speech disability used in Chinese Second Disability Sampling Survey is introduced in the paper,including classification,screening,survey instrument,diagnose,cause analysis and rehabilitation advice.