OBJECTIVE: To investigate the expression of vascular endothelial growth factor in the nasal mucosa of chronic rhinosinusitis with nasal polys patients, and explored the regulation of clarithromycin on VEGF. METHOD: RT-PCR was used to detect the expression of VEGF in nasal mucosa from healthy control and CRSwNP. Nasal mucosal tissue explant culture measure and ELISA were used to explore the effect of clarithromycin on VEGF expression. RESULT: (1) VEGF mRNA expression level was significantly increased in CRSwNP compared with control and showed a statistic difference (P < 0.01). (2) There was a significant decrease in CRSwNP group undergo clarithromycin treatment on protein expression level of VEGF and showed a statistic difference (P < 0.05). CONCLUSION VEGF were overexpressed in CRSwNP group, which presume that play an important role in the pathogenesis of nasal polyps. Clarithromycin may play a therapeutical role on chronic rhinosinusitis through down-regulated the expression of VEGF.
Adult ; Chronic Disease ; Clarithromycin ; pharmacology ; Female ; Humans ; Male ; Nasal Mucosa ; metabolism ; Nasal Polyps ; complications ; Rhinitis ; complications ; metabolism ; Sinusitis ; complications ; metabolism ; Vascular Endothelial Growth Factor A ; metabolism ; Young Adult

Objective To sum up the experience of position nursing of patients with ankylosing spondylitis and severe kyphosis. Methods Eleven patients with ankylosing spondylitis and severe kyphosis underwent surgery. The patients′spine and head were kept in a line during anesthesia to avoid spinal cord injury. The eye mask was used to make sure the eyelid close, so the bulbar conjunctiva and corneal did become dry. The angle of operation bed in time during operation was adjusted. The crushed area was observed and the position was changed to avoid pressure sores and edema. After surgery, they were keep inactive to make sure the spine stable and relieve the pressure. Results The 11 patients′surgery were successful. There were no pressure ulcer, fracture, spinal cord injury or eye injury occurs. The operation time was 9.3 ± 2.9 h. Conclusion Position nursing is very important to improve the success rate of surgery and reduce complications.

Objective To explore the influencing factors on short -term efficacy of intravenous thrombolysis with rt -PA.Methods The clinical data of the 95 acute ischemic stroke(AIS)patients who received thrombolytic therapy were analyze.Multivariate logistic regression analysis was used to determine the possible influencing factors. Results Fifty -six(58.95%)patients had favourable outcomes after thrombolytic therapy for 24 hours.Multivariate logistic regression analysis indicated that diabetes(OR =3.933,95% CI 1.199 ~12.897)and TOAST classification (OR =1.448,95% CI 1.032 ~2.032 )were the independent predictors of short -term outcome.Conclusion Diabetes and TOAST classification are the major influencing factors of short -term efficacy after intravenous thrombolysis with rt -PA.It should pay attention screening patients for intravenous thrombolysis therapy and predicting the efficacy of thrombolysis.

Objective: To evaluate the sensitivity and specificity of exhaled nitric oxide in the diagnosis and therapy of bronchial asthma. Methods: From January 2016 to December 2017, 87 patients diagnosed with bronchial asthma in Luoyang Central Hospital Affiliated to Zhengzhou University were selected as asthma group , and 40 healthy people were selected as healthy control group at the same term.The levels of FeNO, FEV₁/pred, peripheral blood EOS count, ACT score, percentage of sputum EOS were measured. Results: The positive rate of FeNO was 56.3%(49/87) in the asthma group, which in the healthy control group was 5.0%(2/47). The fractional exhaled nitric oxide level of reexamined patients before treatment [(70.9±53.6)ppb] was higher than that after treatment[(12.2±8.7)ppb], and the difference was statistically significant(t=4.323, P=0.001). The fractional exhaled nitric oxide level in the asthma group [(42.4±42.5)ppb] was higher than that in the healthy control group [(9.4±5.8)ppb], and the difference between the two groups was statistically significant(t=2.871, P=0.001). The fractional exhaled nitric oxide level was positively correlated with peripheral blood EOS count (r=0.376, P=0.000) and ACT score (r=0.361, P=0.001), but had no correlation with FEV₁/pred (r=-0.111, P=0.306) and sputum EOS (r=-0.036, P=0.805). Conclusion The measurement of fractional exhaled nitric oxide levels has an important clinical significance in the diagnosis of bronchial asthma.And it has a guiding significance for the evaluation of the therapeutic effect of bronchial asthma.

Objective:To analyze and explore the independent risk factors of 28-day mortality in patients with septic myocardial injury.Methods:A retrospective cohort study was conducted to collect clinical data of 505 patients diagnosed with sepsis related myocardial injury admitted to the intensive care unit (ICU) of the Affiliated Hospital of Jining Medical University from January 2015 to December 2020. According to the 28-day survival status of patients, they were divided into survival group and death group. COX multivariate regression analysis was used to analyze the influencing factors of the 28-day mortality rate of sepsis related myocardial injury patients, and receiver operating characteristic (ROC) curves were drawn to evaluate the effectiveness of independent risk factors in predicting the 28-day mortality rate of sepsis related myocardial injury patients.Results:A total of 505 patients with sepsis myocardial injury were included, of which 282 survived on 28 days and 223 died, with a mortality rate of 44.16%. COX multivariate regression analysis showed that Sequential Organ Failure Assessment (SOFA) score, Acute Physiology and Chronic Health Evaluation Ⅱ (APACHE Ⅱ) score, blood lactate (LAC), oxygenation index (PaO 2/FiO 2), admission heart rate, and albumin were independent risk factors for sepsis associated myocardial injury mortality at 28 days (all P<0.05). ROC curve analysis showed that the area under the ROC curve (AUC) of SOFA score was 0.766 2, and the 95% confidence interval (95% CI) was 0.724 5-0.807 9; The predictive value of 28-day mortality in sepsis associated myocardial injury patients was superior to APACHE Ⅱ score, LAC, PaO 2/FiO 2, admission heart rate, and albumin [The AUC values were 0.754 1(0.711 5-0.796 7), 0.752 6(0.710 1-0.795 1), 0.697 0(0.649 7-0.744 2), 0.623 2(0.573 7-0.672 7), and 0.620 3(0.570 8-0.669 7), respectively]. Conclusions:SOFA score, APACHE Ⅱ score, LAC, PaO 2/FiO 2, admission heart rate, and albumin are independent risk factors for the 28-day mortality rate of sepsis related myocardial injury. Clinical practice should identify these factors early, intervene early, and improve patient prognosis.

Multiple sclerosis(MS)is a systemic inflammatory illness of the central nervous system that involves demyelinating lesions in the myelin-rich white matter and pathology in the grey matter.Despite signifi-cant advancements in drug research for MS,the dis-ease's complex pathophysiology makes it difficult to treat the progressive forms of the disease.In this study,we identified a natural flavonoid compound icariin(ICA)as a potent effective agent for MS in ameliorating the deterioration of symptoms including the neurological defi-cit score and the body weight in a murine experimental autoimmune encephalomyelitis(EAE)model.These improvements were associated with decreased demyelin-ation in the corpus callosum and neuron loss in the hippo-campus and cortex confirmed by immunohistochemistry analysis.Meanwhile,it was observed that the activation of microglia in cerebral cortex and hippocampus were inhibited followed by the neuroinflammatory cytokines downregulation such as IL-1β,IL-6 and TNF-α after ICA treatment,which was probably attributable to the sup-pression of microglial NLRP3 inflammasome activation.Additionally,molecular docking also revealed the binding force of ICA to NLRP3 inflammasome protein complexes in vitro.Taken together,our findings have demonstrated that ICA,as pleiotropic agent,prevents EAE-induced MS by improving demyelination and neuron loss,which inter-feres with the neuroinflammation via microglial NLRP3 inflammasome activation.

Objective:To investigate the effect of berberine on immune regulation and phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) signal pathway in lung cancer rats.Methods:The lung cancer rat model was established by perfusing a carcinogenic lipiodol solution. The 36 male Wistar rats were randomly divided into the model group ( n = 12), the berberine group ( n = 12), and the normal group ( n = 12). The rats in the berberine group were ig berberine 15 mg/kg, once daily. The rats in the model group and the normal group were ig the same dose of normal saline, once daily. The intervention was conducted continuously for 16 weeks for each group. The spleen index and lung index, tumor inhibition rate, T lymphocyte subgroup level, PI3K, and Akt protein expression of rats in each group were compared. Results:The spleen index of the model group and berberine group was lower than that of the normal group, while the lung index was higher than that of the normal group (all P < 0.05). The spleen index of the berberine group was higher than that of the model group, while the lung index was lower than that of the model group (all P < 0.05). The tumor weight of the berberine group was lower than that of the model group ( P < 0.05). The tumor inhibition rate of the berberine group was 43.12%. The CD3 +, CD4 +, and CD4 +/CD8 + levels of the model group and berberine group were lower than those of the normal group, CD8 + level was higher than that of the normal group (all P < 0.05), and the CD3 +, CD4 + and CD4 +/CD8 + levels of the berberine group higher than those of the model group, while CD8 + level was opposite (all P < 0.05). The gray values of PI3K and Akt protein of the model group and berberine group were higher than those of the normal group (all P < 0.05), and this value of the berberine group was lower than that of the model group (all P < 0.05). Conclusions:Berberine can effectively inhibit tumor growth in lung cancer rats, promote spleen development and differentiation, regulate immune function, and downregulate the expression of PI3K/Akt signaling pathway.

Objective To explore the effect and mechanism of microRNA (miR)-17-5p on the invasion and metastasis of hepatocellular carcinoma (HCC) cells. Methods Expression of miR-17-5p in the normal human L-02 hepatocyte and QGY-7703 HCC cells was detected by RT-PCR. QGY-7703 HCC cells were transfected by miR-17-5p mimic and mimic control respectively. Influence of miR-17-5p on the invasion and metastasis ability of HCC cells was detected using Transwell assay and scratch test. Target gene of miR-17-5p was confirmed by bioinformatic analysis, and its expression in HCC cells was detected by Western blot. After siRNA silenced by target gene, the invasion and metastasis ability of HCC cells were observed. Comparison of microRNA in the two kinds of cells was conducted by t test. Results Expression level of miR-17-5p in HCC cells was 0.16±0.04, significantly lower than 1.01±0.19 in normal L-02 hepatocytes (t=-9.67, P<0.05). Number of trans-membrane cells and metastasis rate of HCC cells transfected by miR-17-5p mimic were respectively 36±4 and (5.37±0.15) mm/d, significantly lower than 62±7 and (7.50±0.01) mm/d of control group (t=-15.40, -32.00; P<0.05). Bioinformatic analysis showed that AKT3 was the key target gene of miR-17-5p, and the expression of AKT3 in HCC cells was obviously higher than that of normal hepatocyte. Number of trans-membrane cells and metastasis rate of HCC cells transfected by siRNA-AKT3 were respectively 13±3 and (4.13±0.15) mm/d, significantly lower than 58±3 and (7.23±0.25) mm/d of control group (t=-17.88, -53.69; P<0.05). Conclusion miR-17-5p inhibits the invasion and metastasis ability of HCC cells through targeting effect on AKT3.

Objective:This article conducted a metrology analysis of the literatures about orthopaedic rehabilitation, and to understand the research status, hot spot and advanced trend of orthopedic rehabilitation.Methods:Literatures related to orthopedic rehabilitation were retrieved from Web of Science core collection database from January 1, 2017 to March 30, 2022. CiteSpace 5.8. R3 software was used to analyze the countries, institutions, keyword co-occurrence, keyword clustering, keyword emergence and so on.Results:A total of 918 literatures were included, and the number of publications was relatively stable in the past 5 years. The United States was the country with the largest number of publications, Monash University was the research institution with the largest number of publications, and the journal with the highest citation frequency was J Bone Joint Surg Am. Burst keywords "questionnaire" and "predictor" would become the development trend and research focus in this field. Conclusions:The literatures related to orthopedic rehabilitation cover a wide range, but the depth of the research is insufficient, the published journals are scattered, and the cooperation between the state and institutions needs to be strengthened.

Objective To explore the effects of sound operation on nursing quality in nursing work at spine surgery.Methods Totally 531 cases of patients at spine surgery in our hospital from July 2015 to October 2015 were randomly divided into the control group ( n=264) and the intervention group ( n=267) . Patients in two groups were given routine nursing, and patients in the intervention group also were given sound operation. The incidence of nursing adverse events, the writing quality of nursing documents, the situation of health education, the rate of patient complaints and the satisfaction degree toward nursing were collected and analyzed. Results Nursing adverse events in the intervention group was 1 case ( 0. 4%) which were less than 11 cases (4.2%) in the control group (P<0.05). There were 2 cases (0.7%) of nonstandard nursing documents and 3 cases (1.1%) of lipography in nursing documents in the intervention group, which were less than 11 cases (4.2%) and 16 cases (6.1%) in the control group (P<0.05). There was no case of patient complaints in the intervention group which were less than 4 cases (1.5%) in the control group (P<0.05). The situation of health education in the intervention group was 1 case (0.4%) which was less than 8 cases (3.0%) in the control group (P<0.05). The satisfaction degree toward nursing in the intervention group was 97.75%(261/267)and was 92.80%(245/264) in the control group ( P<0. 05). Conclusions Sound operation in nursing work can effectively reduce nursing adverse events at spine surgery, and it also can improve the quality of nursing.